Acute Deficiency Research Articles

Combined effects of salinity and intermittent hypoxia on mitochondrial capacity and reactive oxygen species efflux in the Pacific oyster, Crassostrea gigas.

Coastal environments commonly experience fluctuations in salinity and hypoxia-reoxygenation (H/R) stress that can negatively affect mitochondrial functions of marine organisms. Although intertidal bivalves are adapted to these conditions, the mechanisms that sustain mitochondrial integrity and function are not well understood. We determined the rates of respiration and reactive oxygen species (ROS) efflux in the mitochondria of oysters, Crassostrea gigas, acclimated to high (33 psu) or low (15 psu) salinity, and exposed to either normoxic conditions (control; 21% O2) or short-term hypoxia (24 h at <0.01% O2) and subsequent reoxygenation (1.5 h at 21% O2). Further, we exposed isolated mitochondria to anoxia in vitro to assess their ability to recover from acute (∼10 min) oxygen deficiency (<0.01% O2). Our results showed that mitochondria of oysters acclimated to high or low salinity did not show severe damage and dysfunction during H/R stress, consistent with the hypoxia tolerance of C. gigas. However, acclimation to low salinity led to improved mitochondrial performance and plasticity, indicating that 15psu might be closer to the metabolic optimum of C. gigas than 33psu. Thus, acclimation to low salinity increased mitochondrial oxidative phosphorylation rate and coupling efficiency and stimulated mitochondrial respiration after acute H/R stress. However, elevated ROS efflux in the mitochondria of low-salinity-acclimated oysters after acute H/R stress indicates a possible trade-off of higher respiration. The high plasticity and stress tolerance of C. gigas mitochondria may contribute to the success of this invasive species and facilitate its further expansion into brackish regions such as the Baltic Sea.

Formulation Of Herbal Cream to Treat Dry Skin

The main aim of the present article is to reduce the adverse effect associated with synthetic dry skin cream by formulating an herbal dry skin cream for treatment of dry skin. Abnormally dry skin Can be occurring due to a dry weather, winter weather, acute deficiency of vitamin A, systemic illness, over exposure to sunlight, or medication. The skin loses moisture. It may crack and peel. Or it may become irritated, inflamed, and itch. Bathing repeatedly, especially with soaps, can contribute to dry skin. This research mainly based on the treatment of dry skin by using various herbs and making poly herbal formulation. Herbs such as cucumis sativus (main fruit juice) used as repair dry skin and damaged skin, moisturizer, azadirachta indica (neem leaves) used as skin moisturizer, rose water(rose water) used as cooling agent, flavouring agent, emollient, tulasi (tulasi leaves) used as antioxidant and skin texture improve, antimicrobial agent, amla (amla fruit) used as antioxidant and vitamin C help to brighten your skin, vitamin E used as moisturizer skin, lotus flower(lotus petals) used as anti-inflammatory and which help balance sebum production, improve the texture and elasticity of the skin, cool. Natural herbal extract in combination effectively utilized for the treatment of dry skin. Use of such product far better than the use of synthetic cream for the control of dry skin.

Applying the theory of planned behavior for prediction of oocyte donation intention among Iranian women

BackgroundOocyte donation is a complex and multifaceted behavior in women. Due to the acute deficiency of donated oocytes, it is necessary to identify the factors affecting the desire to donate. Planned behavior theory is a suitable model for explaining and predicting behavior in many behavioral domains. The aim of this study was to predict oocyte donation intention using the theory of planned behavior.MethodsThis cross-sectional study was performed on 556 women who were covered by the comprehensive health service centers in Gonabad and Neyshabour cities in 2020. Sampling was performed by the two-step method in Gonabad city and by the convenience sampling method in Neyshabour city. Data were collected online using researcher-made questionnaires included oocyte donation awareness questionnaire and oocyte donation intention questionnaire (based on the TPB constructs). The validity of the questionnaires was confirmed by face and content validity and its reliability was confirmed by Cronbach's alpha coefficient. Data were analyzed in SPSS software (version 16) using hierarchical linear regression. The statistical significance was considered as P < 0.05.ResultsThe results showed that there was a significant positive correlation between the oocyte donation intention with all constructs of planned behavioral theory (P < 0.050). Planned behavior theory constructs explained 47% of the variance of oocyte donation intention. In addition, constructs of perceived power (β = 0.461), control beliefs (β = 0.154) and normative beliefs (β = 0.125) were the strongest predictors of oocyte donation intention, respectively.ConclusionPlanning and implementing educational courses according to the constructs of the theory of planned behavior can be a great step toward the elimination of oocyte deficiency and infertility problems. Also performing of intervention or training strategies in the field of oocyte donation requires the enhancement of social norms and perceived behavioral control in the population under study.

Descriptive spectrum of thiamine deficiency in pregnancy: Apotentially preventable condition.

Pregnancy, a nutritionally demanding situation in terms of macro- and micronutrient supply owing to heightened maternal, placental, and fetal needs, significantly affects thiamine reserves. Thiamine deficiency during pregnancy and the postpartum period, presenting with varied manifestations and outcomes, is a relatively common condition in our population. The study aimed to understand the various manifestations and outcomes of acute thiamine deficiency in pregnant and postpartum women, emphasizing the significance of early recognition and thiamine therapy to prevent serious complications during pregnancy and after childbirth. This prospective study conducted in a tertiary care center in North India enrolled consecutive pregnant and postpartum women presenting with clinical features consistent with thiamine deficiency disorders, such as thiamine deficiency-related neuropathy, high-output heart failure, heart failure with reduced ejection fraction, Wernicke's encephalopathy, gastric beriberi, and thiamine-responsive acute pulmonary hypertension. In addition to capturing medical history including drug intake, dietary consumption, and comorbidities, women underwent brief relevant clinical examinations and laboratory assessments, including whole-blood thiamine levels. Response to intravenous thiamine supplementation was also monitored. Data of 31 women (12 pregnant, 19 postpartum) with a diagnosis of acute thiamine deficiency and a mean age of 28.88 ± 2.69 years were analyzed. The mean thiamine level was 1.28 ± 0.44 μg/dL with mean blood lactate of 3.46 ± 3.33. The most common presentation was gastric beriberi (n = 10), followed by paraparesis (n = 6), high-output heart failure (n = 6), acute pulmonary hypertension, heart failure with reduced ejection fraction (n = 3 each), and an acute confusional state (n = 2). All patients responded to thiamine challenge. In the context of borderline thiamine status, particularly in our population with endemic thiamine deficiency and heightened demand for thiamine during pregnancy and the peripartum period, the deficiency can have varied and serious manifestations of dry and wet beriberi. Early recognition of the clinical features and thiamine therapy can be life-saving. There is a need for validated clinical criteria owing to the non-availability of thiamine testing in resource-limited settings.

Identification of hypoxia-related genes and exploration of their relationship with immune cells in ischemic stroke

Ischemic stroke (IS) is a major threat to human health, and it is the second leading cause of long-term disability and death in the world. Impaired cerebral perfusion leads to acute hypoxia and glucose deficiency, which in turn induces a stroke cascade response that ultimately leads to cell death. Screening and identifying hypoxia-related genes (HRGs) and therapeutic targets is important for neuroprotection before and during brain recanalization to protect against injury and extend the time window to further improve functional outcomes before pharmacological and mechanical thrombolysis. First, we downloaded the GSE16561 and GSE58294 datasets from the NCBI GEO database. Bioinformatics analysis of the GSE16561 dataset using the limma package identified differentially expressed genes (DEGs) in ischemic stroke using adj. p. values < 0.05 and a fold change of 0.5 as thresholds. The Molecular Signature database and Genecards database were pooled to obtain hypoxia-related genes. 19 HRGs associated with ischemic stroke were obtained after taking the intersection. LASSO regression and multivariate logistic regression were applied to identify critical biomarkers with independent diagnostic values. ROC curves were constructed to validate their diagnostic efficacy. We used CIBERSORT to analyze the differences in the immune microenvironment between IS patients and controls. Finally, we investigated the correlation between HRGs and infiltrating immune cells to understand molecular immune mechanisms better. Our study analyzed the role of HRGs in ischemic stroke. Nineteen hypoxia-related genes were obtained. Enrichment analysis showed that 19 HRGs were involved in response to hypoxia, HIF-1 signaling pathway, autophagy, autophagy of mitochondrion, and AMPK signaling pathway. Because of the good diagnostic properties of SLC2A3, we further investigated the function of SLC2A3 and found that it is closely related to immunity. We have also explored the relevance of other critical genes to immune cells. Our findings suggest that hypoxia-related genes play a crucial role in the diversity and complexity of the IS immune microenvironment. Exploring the association between hypoxia-related critical genes and immune cells provides innovative insights into the therapeutic targets for ischemic stroke.

Acute Thiamine Deficiency Detected by Video Head Impulse Test in a Patient with Gastric Mucosa-Associated Lymphoid Tissue (MALT) Lymphoma

Thiamine deficiency may cause Wernicke encephalopathy (WE) characterized by ataxia, ophthalmoplegia, and confusion. Aside from the triad of cardinal symptoms, selective or predominant impairments of bilateral horizontal canals may be observed in WE. Here, we report a patient with gastric mucosa-associated lymphoid tissue (MALT)-lymphoma complicated by an early stage of WE due to thiamine deficiency manifesting bilateral vestibular dysfunctions. A 78-year-old man recently diagnosed gastric MALT-lymphoma presented with dizziness and disequilibrium for several weeks. He showed mild imbalance while tandem walking and abnormal catch-up saccades bilaterally during bedside head impulse test (HIT). Video HIT revealed decreased vestibulo-ocular reflex gains with catch-up saccades for the bilateral horizontal and several vertical canals. Diagnostic work-up demonstrated decreased serum thiamine and lesions in the mammillary body and periaqueductal gray matter on brain magnetic resonance images. Furthermore, with thiamine replacement, his clinical symptoms were markedly improved. Also, vestibular symptoms and signs may be an early manifestation of WE, and video HIT could be a useful diagnostic tool to aid early detections.

Discussion of Acute Deficiency Syndrome Theory and its Clinical Application

Discussion of Acute Deficiency Syndrome Theory and its Clinical Application

Genetic studies on iron and zinc concentrations in common bean (Phaseolus vulgaris L.) in Ghana

Iron and zinc deficiencies cause high health risk to young children and expectant mothers in sub Saharan Africa. The development of biofortified common bean (Phaseolus vulgaris L.) varieties could address the acute micronutrient deficiencies with associated improvement in the nutrition and health of women, children and adults. The objective of this study was to determine the mode of gene action and genetic advance in iron and zinc levels in common bean. Field experiment was carried out using six generations of two populations made of crosses between pairs of low iron, low zinc and high iron, moderate zinc genotypes (Cal 96 ˣ RWR 2154; MCR-ISD-672 ˣ RWR 2154). Each generation (P1, P2, F1, F2, BC1P1 and BC1P2) was evaluated on the field in a randomized complete block design with three replications. Generation mean analysis were performed for each trait measured in each of the crosses while iron and zinc levels were quantified by x-ray fluorescence. The study showed that both additive and non-additive gene effects were important in determining the expression of high iron and zinc levels. Iron concentration in the common bean seeds ranged from 60.68 to 101.66 ppm while zinc levels ranged from 25.87 to 34.04 ppm. Broad sense heritability estimates of iron and zinc were high in the two crosses (62–82% for Fe and 60–74% for Zn) while narrow sense heritability ranged from low to high (53–75% for Fe and 21–46% for Zn). Heritability and genetic gain were used as selection criteria for iron and zinc, and it was concluded that doing so would be beneficial for future improvement.

Challenge in modern intensive care: chronic critical illness - pathophysiology and therapeutic options

The recent developments in intensive care have resulted in improved survival rates of patients treated with acute organ deficiency. As a consequence, the rate of those who survive the acute phase and subsequently require protracted organ support due to persisting organ dysfunction has been growing. Several survivors display chronic health status deterioration leading to prolonged rehabilitation or nursing, and repeated hospitalizations. The condition developed following the survival of the acute phase and requiring long-lasting intensive care is frequently termed as chronic critical illness (CCI). Several definitions exist, most of these are based on the number of ventilator days, or days of stay at the intensive care unit. Nevertheless, in spite of the initially heterogenous etiology of the acute illness, the complications associated with CCI, as well as the pathophysiological processes underlying these, are relatively uniform. This causes CCI to be a unique clinical syndrome characterized by the development of secondary infections, myopathy, central and peripheral neuropathy, and typical alterations of the hormonal and immune system functions. The outcome is heavily influenced by the frailty and comorbidities of the patient, in addition to the severity of the acute illness. The treatment of CCI patients presents a complicated task requiring multidisciplinary view and individualized therapeutic measures. Since the aging of the population and the continuously improving success rates in overcoming acute conditions also facilitate the development of CCI, the systematic overview of the underlying pathophysiological processes is pivotal for the optimization of the medical, nursing, social and economical burden presented by this syndrome. Orv Hetil. 2023; 164(18): 702-712.

ACUTE SURGICAL ABDOMEN: UNEXPECTED MEDICAL CAUSE

Acute surgical abdomen is a diagnostic and therapeutic emergency, manifested by a generalized contracture of the abdominal muscles following a peritoneal irritation. In the majority of cases, acute peritonitis is the most common etiology requiring urgent surgical intervention. Nevertheless, some medical pathologies, notably acute adrenal deficiency and inflammatory colitis, can mimic this surgical situation, leading to an aberrant surgical intervention. Through a clinical case, we report the diagnostic and therapeutic modalities of a multifocal tuberculosis initially admitted for an acute surgical abdomen.

Sleep, Immune Function, and Vaccinations in Military Personnel: Challenges and Future Directions.

The U.S. military invests substantial resources to vaccinate all personnel, including recruits, against operationally important infectious disease threats. However, research suggests that vaccine immune response and, therefore, vaccine effectiveness may be inadvertently reduced because of chronic and/or acute sleep deficiency experienced by recipients around the time of vaccination. Because sleep deficiency is expected and even necessary in deployed and training contexts, research investigations of the impacts of sleep and related physiological systems such as circadian rhythms on vaccine effectiveness in military settings are needed. Specifically, research should be aimed at understanding the effects of sleep deficiency, as well as vaccine administration schedules, on response to vaccination and clinical protection. Furthermore, knowledge gaps among military medical leadership on sleep, vaccines, and immune health should be assessed. This area of research may benefit the health and readiness of service members while also decreasing health care utilization and associated costs from illness.

Orexin induces the production of an endocannabinoid-derived lysophosphatidic acid eliciting hypothalamic synaptic loss in obesity

ObjectiveOrexin-A (OX-A) is a neuropeptide produced selectively by neurons of the lateral hypothalamus. It exerts powerful control over brain function and physiology by regulating energy homeostasis and complex behaviors linked to arousal. Under conditions of chronic or acute brain leptin signaling deficiency, such as in obesity or short-term food deprivation, respectively, OX-A neurons become hyperactive and promote hyperarousal and food seeking. However, this leptin-dependent mechanism is still mostly unexplored. The endocannabinoid 2-arachidonoyl-glycerol (2-AG) is known to be implicated in food consumption by promoting hyperphagia and obesity, and we and others demonstrated that OX-A is a strong inducer of 2-AG biosynthesis. Here, we investigated the hypothesis that, under acute (6 h fasting in wt mice) or chronic (in ob/ob mice) hypothalamic leptin signaling reduction, OX-A-induced enhancement of 2-AG levels leads to the production of the 2-AG-derived 2-arachidonoyl-sn-glycerol-3-phosphate (2-AGP), a bioactive lipid belonging to the class of lysophosphatidic acids (LPAs), which then regulates hypothalamic synaptic plasticity by disassembling α-MSH anorexigenic inputs via GSK-3β-mediated Tau phosphorylation, ultimately affecting food intake. MethodsWe combined cell-type-specific morphological (CLEM and confocal microscopy), biochemical, pharmacological, and electrophysiological techniques to dissect the leptin- and OX-A/2-AGP-mediated molecular pathways regulating GSK-3β-controlled pT231-Tau production at POMC neurons of obese ob/ob and wild-type (wt) lean littermate mice and in an in vitro model of POMC neurons such as mHypoN41 neurons (N41). Results2-AGP is overproduced in the hypothalamus of obese leptin-deficient, or lean 6 h food-deprived mice, and promotes food intake by reducing α-MSH-expressing synaptic inputs to OX-A neurons via lysophosphatidic acid type-1 receptor (LPA1-R) activation, and pT231-Tau accumulation in α-MSH projections. This effect is due to the activation of the Pyk2-mediated pTyr216-GSK3β pathway and contributes to further elevating OX-A release in obesity. Accordingly, we found a strong correlation between OX-A and 2-AGP levels in the serum of obese mice and of human subjects. ConclusionsHypothalamic feeding pathways are endowed with 2-AGP-mediated synaptic plasticity according to their inherent functional activities and the necessity to adapt to changes in the nutritional status. These findings reveal a new molecular pathway involved in energy homeostasis regulation, which could be targeted to treat obesity and related disturbances.

Effect of Methionine and Choline Deficiency on Liver Choline Oxidase Activity in Young Rats

Young male Sprague-Dawley rats were fed a water-soluble chemically defined diet containing 0.06, 0.12, 0.24 or 0.60% methionine with or without 0.25% choline for 10 to 42 days. Liver choline oxidase activity decreased in rats that ingested the choline-deficient diets (devoid of choline and low (0.06 or 0.12%) in methionine). These animals developed pathologic changes in the liver and kidney characteristic of acute choline deficiency. Liver choline oxidase activity remained normal in rats that were fed the methionine-deficient diets (low in methionine but supplemented with 0.25% choline).

Modeling of Cerebral Anoxia of Respiratory Genesis in Rats

Anoxia as an extreme degree of acute oxygen starvation of the brain cells, regardless of the causes, leads to its greatest damage. As a result of using a new model of cerebral anoxia we have established disturbances in the parietal cortex of the rat brain, manifested by changes in the size, shape of neurons, the degree of their chromatophilia. This indicates the possibility of using the method of modeling anoxia by tracheal compression to study acute cerebral oxygen deficiency. It provides an opportunity to assess visually the dynamics of neuronal changes under anoxia without shutting down the cerebral circulation. It may be of value for further study of the consequences of disorders in the brain arising from respiratory pathology and external causes accompanied by asphyxia.

Brain Iron, Transferrin and Ferritin Concentrations Are Altered in Developing Iron-Deficient Rats

To study the iron, transferrin, and ferritin distribution at subcellular levels in response to acute dietary iron deficiency, we tested the hypothesis that early post-weaning iron deficiency can change iron and iron regulatory protein concentrations in rat brain. Male Sprague-Dawley rats were fed diets containing either 2 or 35 µg iron/g for 2, 3 or 4 wk starting at 21 d of age. Brain iron, transferrin and ferritin concentrations in cytosolic and microsomal fractions of either whole brain or pons and cerebellum were then determined. After 14 d of dietary iron restriction, brain iron concentrations were 50% lower in the microsomal fraction and 30% lower in cytosol compared with controls. Brain cytosolic transferrin concentration almost doubled in the same animals. Brain ferritin concentration in fractions from rats fed the iron-deficient diet for 14 d was lower than in controls, but then remained fairly constant. Absolute brain weight and total brain protein contents were unaffected by iron restriction. This study extends previous research by demonstrating that the brain responds to changes in body iron status with a change in transferrin concentration. If the dietary restriction is quite severe, this adaptation is insufficient. This study also notes that brain ferritin decreases with decreasing body iron status, though it was less responsive than nonheme iron in liver. The concept that iron enters the brain through a highly regulated endocytotic process at the blood brain barrier, that undoubtedly involves the regulation of transferrin receptors in capillary endothelial cell, is supported by our observation of elevated transferrin concentrations in brain of iron-deficient rats.

The Role of Hypoxia-Induced Factor in Cell Metabolism

Objective. Analysis and synthesis of literature data on morphological and functional properties and the diagnostic value of induced factor hypoxia. Methods. The basis of this study was a review of literature on this topic. Results. An important role in the adaptation of the organism to hypoxia belongs to a specific regulatory protein - hypoxia-induced factor (HIF), the activity of which increases with decreasing oxygen tension in the blood. HIF is a heterodimeric protein, the beta subunit of which is constantly expressed, and the synthesis of the alpha subunit is regulated by oxygen. Conclusion. Acute oxygen deficiency is the basis of a variety of pathological processes in many diseases and environmental factors. The hypoxia-induced factor is responsible for the formation of long-term adaptation to hypoxia, and therefore is a suitable target for pharmacological effects. The search for drugs that act as inducers or inhibitors of its synthesis is an important area in experimental pharmacology, since it allows not only to regulate the processes of adaptation to hypoxia, but more effectively treat cerebrovascular, cardiovascular, oncological and other diseases in whose genesis the leading role plays oxygen deficiency.

Assessment of the effect of iron-rich humic substances on hematological parameters in the model of acute posthemorrhagic and iron deficiency anemia

Aim. To assess the effect of iron-rich humic substances on hematological parameters in acute post-hemorrhagic and iron deficiency anemia.Materials and methods. Materials for the study were samples of iron-rich active pharmaceutical ingredients based on humic substances (Fe(III) hydroxide complexes with humic substances and polymaltose): HA-Fe3+, HA-PMFe3+, FA-Fe3+, and FA-PM-Fe3+. The anti-anemic activity of the substances was studied on 53 female Wistar rats of the conventional rat line in the model of acute posthemorrhagic and iron deficiency anemia. Anti-anemic activity was assessed by the hemoglobin level, erythrocyte count, hematocrit, and serum iron level.Results. The studied substances HA-Fe3+ and FA-Fe3+ are the most effective in correcting the consequences of both experimental acute posthemorrhagic anemia and iron deficiency anemia. Their effect is comparable to that of the positive control drug Ferrum Lek.Conclusion. Fe(III) hydroxide complexes stabilized by humic and fulvic acids exhibit anti-anemic activity.

Socio-demographic Factors and Household Expenditure on Food Consumption in Ondo State, Nigeria

Household expenditure on food consumption has become an issue of great concern in Nigeria today. This is so because an increasing percentage of the household in Nigeria was feeding less and therefore become more food insecure which made households suffer serious ailments arising from an acute deficiency in their diets. This study examined the effect of socioeconomic factors on household expenditure on food consumption in Nigeria. Data were gotten through the administration of questionnaires to 2500 household heads in three senatorial districts in Ondo State. The study adopted multistage sample techniques; we analyzed information extracted from the questionnaire using both descriptive and regression techniques. The result indicated that major source of income exerts a significant negative (t=-3.76, p&lt;0.05) effect on household consumption expenditure in Ondo State, Nigeria, a positive relationship between household size and consumption expenditure (t=5,38, p&lt;0.05), and a positive impact between education qualification and household expenditure for consumption (t-3.26,p&lt;0.05). However, gender, age, marital status, and respondents' senatorial districts did not get a significant effect on households' consumption expenditure of food. The study recommended that government should try to pay workers' salaries as at when due to discourage reduction in food consumption expenditure of the people

Comparative Characteristics of Morphological Changes in Neurons of The Parietal Cortex of Rats with Anoxia of Ischemic and Respiratory Genesis

Acute oxygen deficiency is the basis of a variety of pathological processes in many diseases and environmental factors. When studying the rat brain under conditions of its total ischemia and mechanical asphyxia, the presence of structural changes in both studied periods (after 30 and 60 minutes) was revealed: a decrease in the area and a change in shape (loss of sphericity and an increase in elongation) of cells, as well as a change in the degree of chromatophilia, which was manifested by a decrease in normochromic neurons with a simultaneous increase in hyperchromic shriveled neurons. At the same time, total cerebral ischemia led to more pronounced changes in the studied neurons of the parietal cortex, which was manifested by a more significant decrease in the area of neurons after 30 minutes of anoxia, while shape changes in the form of loss of sphericity and increased elongation were noted to a greater extent with mechanical asphyxia. These differences may be due to the preservation of cardiac activity during mechanical asphyxia for a short time.

Inhibition of PDE10A in a New Rat Model of Severe Dopamine Depletion Suggests New Approach to Non-Dopamine Parkinson’s Disease Therapy

Parkinson's disease is the second most common neurodegenerative pathology. Due to the limitations of existing therapeutic approaches, novel anti-parkinsonian medicines with non-dopamine mechanisms of action are clearly needed. One of the promising pharmacological targets for anti-Parkinson drug development is phosphodiesterase (PDE) 10A. The stimulating motor effects of PDE10A inhibition were detected only under the conditions of partial dopamine depletion. The results raise the question of whether PDE10A inhibitors are able to restore locomotor activity when dopamine levels are very low. To address this issue, we (1) developed and validated the rat model of acute severe dopamine deficiency and (2) tested the action of PDE10A inhibitor MP-10 in this model. All experiments were performed in dopamine transporter knockout (DAT-KO) rats. A tyrosine hydroxylase inhibitor, α-Methyl-DL-tyrosine (αMPT), was used as an agent to cause extreme dopamine deficiency. In vivo tests included estimation of locomotor activity and catalepsy levels in the bar test. Additionally, we evaluated the tissue content of dopamine in brain samples by HPLC analysis. The acute administration of αMPT to DAT-KO rats caused severe depletion of dopamine, immobility, and catalepsy (Dopamine-Deficient DAT-KO (DDD) rats). As expected, treatment with the L-DOPA and carbidopa combination restored the motor functions of DDD rats. Strikingly, administration of MP-10 also fully reversed immobility and catalepsy in DDD rats. According to neurochemical studies, the action of MP-10, in contrast to L-DOPA + carbidopa, seems to be dopamine-independent. These observations indicate that targeting PDE10A may represent a new promising approach in the development of non-dopamine therapies for Parkinson's disease.