Abstract
Young male Sprague-Dawley rats were fed a water-soluble chemically defined diet containing 0.06, 0.12, 0.24 or 0.60% methionine with or without 0.25% choline for 10 to 42 days. Liver choline oxidase activity decreased in rats that ingested the choline-deficient diets (devoid of choline and low (0.06 or 0.12%) in methionine). These animals developed pathologic changes in the liver and kidney characteristic of acute choline deficiency. Liver choline oxidase activity remained normal in rats that were fed the methionine-deficient diets (low in methionine but supplemented with 0.25% choline).
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