You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Neurogenic Voiding Dysfunction1 Apr 20101001 DIMINISHED DETRUSOR MUSCLE CONTRACTILITY UNDERLIES THE URINARY BLADDER DYSFUNCTION INDUCED BY EXPERIMENTAL COLITIS: MECHANISM FOR PELVIC ORGAN CROSS-TALK Tirsit Asfaw, Joseph Hypolite, Gina Northington, Lily Arya, Alan Wein, and Anna Malykhina Tirsit AsfawTirsit Asfaw Philadelphia, PA More articles by this author , Joseph HypoliteJoseph Hypolite Glenolden, PA More articles by this author , Gina NorthingtonGina Northington Philadelphia, PA More articles by this author , Lily AryaLily Arya Philadelphia, PA More articles by this author , Alan WeinAlan Wein Glenolden, PA More articles by this author , and Anna MalykhinaAnna Malykhina Glenolden, PA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.2015AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Chronic pelvic pain is a common symptom of many gynecologic, urological and gastrointestinal conditions. Previous studies have demonstrated viscerovisceral cross-sensitization among pelvic organs via hyperexcitability of sensory and spinal neurons and the role of TRPV1 signaling in the development of neurogenic inflammation in the pelvis. The objective of this study was to elucidate the effects of desensitization of TRPV1 receptors in the colon on in vivo and in vitro contractility of the urinary bladder smooth muscle after experimentally induced colitis. METHODS Four groups of animals (males, Sprague-Dawley rats) were included in the study: 1- control group (intracolonic saline), 2 - trinitrobenzene sulfonic acid (TNBS) induced colitis, 3 - intracolonic resiniferatoxin (RTX) instillation and 4 - intracolonic RTX followed by TNBS. The contractility of detrusor muscle was studied in vivo using awake animal cystometry and in vitro by measuring the contractile response of isolated muscle strips in organ bath setting. Cystometry was performed at baseline and 3 days post treatment while organ bath experiments were performed 3 days post treatment in all groups. The organ bath studies were performed on colonic (positive control) and bladder strips using electric field stimulation (EFS), potassium chloride test (KCL) and carbachol dose response stimulation. Maximum value (MV, g/mg) and maximum slope (MS, g/s) of the contractile response were compared among the groups. RESULTS Acute colonic inflammation significantly decreased the response of the bladder smooth muscle to EFS (32 Hz) by almost 2-fold compared to control group (n=7, p≤0.05). In parallel, the velocity of the response was slowed down by experimental colitis from 1.1±0.5 g/s to 2.2±0.6 g/s (n=7, p≤0.05). These changes were accompanied by a decrease of MV in the distal colon directly affected by inflammation. Desensitization of colonic TRPV1 receptors did not have significant impact on the contractility of the detrusor muscle. Responses to EFS, KCl and carbachol were also significantly lower in RTX+TNBS group compared to the control. The results of cystometry on awake and freely moving animals showed marked difference in the bladder volume and pressure at micturition in animals with acute experimental colitis. CONCLUSIONS Our results provide evidence that acute inflammation in the distal colon modifies the function of the urinary bladder via changes in detrusor muscle contractility. © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e389 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Tirsit Asfaw Philadelphia, PA More articles by this author Joseph Hypolite Glenolden, PA More articles by this author Gina Northington Philadelphia, PA More articles by this author Lily Arya Philadelphia, PA More articles by this author Alan Wein Glenolden, PA More articles by this author Anna Malykhina Glenolden, PA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...