Activity Promotion Research Articles

Sustainable Practices as an Incentive to Return to Play at in an International Event

Objective: The aim of this study is to investigate the effect that the implementation of green practices in a major sport tourism event, the Badminton World Championships in Huelva (Spain), has on the future intention of similar players to return to compete in sport events. Theoretical Framework: This theme introduces the main concepts and theories underpinning the research. Green practices, perceived value, satisfaction, trust and emotions are highlighted, providing a solid basis for understanding the context of the research. Method: The methodology adopted for this research comprised a total of 400 players who attended to compete in the event were randomly selected. A confirmatory factor analysis of the model and a multigroup analysis were carried out. Data collection was carried out by means of a self-administered questionnaire in the presence of the interviewer. Results and Discussion: The results obtained revealed that green practices are a direct predictor of confidence, emotions and perceived value, thus influencing satisfaction and intention to attend or re-attend a similar sporting event as a player. Research implications: Implementing green practices can be an effective strategy to improve participant satisfaction and confidence, which in turn can increase intentions to return to compete in future events. Organisers, federations, and sport tourism managers should consider including measures that promote environmental sustainability, such as waste reduction, efficient use of resources, and promotion of sustainable activities among participants. Originality/value: This study contributes to the literature by understanding the impact of green practices on participants' satisfaction, confidence and intention to return to sporting events. The relevance and value of this research is evidenced by how these practices can influence the behaviour of sport tourists, filling an important gap in the existing literature on sport tourism and sustainability.

Effect of muscle strength on deep vein thrombosis: A Mendelian randomization study

Deep vein thrombosis (DVT) is a serious condition characterized by blood clots in deep veins, posing a significant public health burden. Muscle strength has been implicated as a potential risk factor for DVT due to its influence on venous return. This study aims to investigate the causal association between muscle strength and DVT using a Mendelian randomization (MR) approach, leveraging genetic variants as instrumental variables (IVs). We conducted a 2-sample MR analysis using genome-wide association study (GWAS) data for hand-grip strength and DVT. IVs were selected based on their significant associations with muscle strength and DVT, as well as their linkage disequilibrium patterns. We employed statistical methods including inverse-variance weighting (IVW), MR-Egger, and weighted median to address pleiotropy bias. Sensitivity analyses were conducted to evaluate the robustness of the results. A total of 21 and 14 independent IVs were identified for hand grip strength (EWGSOP) and hand grip strength (FNIH), respectively. IVW analysis revealed a consistent causal and negative association between both definitions of hand grip strength and DVT (EWGSOP: OR = 0.702, 95% CI: 0.511–0.964, P = .029; FNIH: OR = 0.715, 95% CI: 0.570–0.898, P = .004). No directional pleiotropy was detected in MR-Egger and MR-PRESSO analyses for either definition (EWGSOP: MR-Egger Intercept P = .516; MR-PRESSO global test P = .162; FNIH: MR-Egger Intercept P = .569; MR-PRESSO global test P = .371).Sensitivity analyses demonstrated the stability of the causal effect estimates, with little influence from individual IVs. The MR analysis provided evidence of a causal association between muscle strength and DVT risk, suggesting that increasing muscle strength may have a protective effect. These findings have implications for preventive strategies and the promotion of resistance exercises and muscle-strengthening activities. Further research and validation of these results could inform clinical guidelines and interventions for DVT prevention.

Physical fitness and body build parameters of children and adolescents participating in the physical activity promotion programme “Athletics for all!”

BackgroundThis study attempts to analyse physical fitness and basic anthropometric parameters of children and adolescents participating in the nationwide physical activity promotion programme “Athletics for All!” (AFA). The programme aims to establish a national system for diagnosing, selecting, recruiting, and identifying talents in youth training. It also aims to build a career development path for athletics, forming the basis of a new structure for youth training in Poland. The primary goal of this study was to assess physical fitness of AFA participants over the years of its implementation (2015–2022) and to identify the leading motor ability with consideration for age, gender, and body build parameters determined with Body Mass Index and Ponderal Index.MethodsNationwide measurements of basic anthropometric parameters as well as physical fitness tests were conducted among 31,790 girls (F) and 22,260 boys (M) participating in the AFA programme. Physical fitness assessments were performed using the OSF test (3 × 10 m shuttle run, standing broad jump, 1 kg medicine ball throw, 4-minute run). Comparative analysis of OSF test results, considering gender, was conducted using the independent samples T-test. The strength and direction of correlations between variables were calculated using the rho-Spearman coefficient.ResultsOSF test results were converted into points, considering age and gender. Gender was found to be a significantly differentiating factor in physical fitness of AFA participants. Among twelve- and fifteen-year-olds, statistically significant differences were observed in all analysed variables, i.e. in each of the four tests and the overall score. It was noted that the examined girls aged 11 to 17 exhibited higher levels of strength compared to their male counterparts. Statistical analysis revealed significant correlations between OSF test results and the age and body build indicators, with the strength of the correlations being negligible in most cases.ConclusionsParticipants of the nationwide programme “Athletics for All!” demonstrate a high level of physical fitness, with endurance being a fundamental motor ability. Physical fitness levels show significant correlations with the anthropometric parameters of children and adolescents. The study results confirm the need and justification for implementing physical activity promotion programmes for the younger generation.

Empirical Study on the Pottery Environment and its Contribution to the Economic, Cultural and Institutional Growth of the Community of Huancas, Amazonas, Peru: An Analysis with Smart Pls

This study focuses on analyzing how the pottery environment contributes to the economic, cultural and institutional growth of the community of Huancas, Amazonas, Peru. The research has a non-experimental approach at a descriptive correlational level. A total of 35 pottery families participated in the survey and the data collected were analyzed in SPSS and Smart PLS 4.0 statistical programs. The results indicate that the pottery field in the community of Huancas has contributed positively to economic, cultural and institutional growth (0.942; 0.935 and 0.915) respectively; this work represents the cultural identity of the community, based on the regular production of pots for cooking food and a great variety of vessels, with women being the main protagonists of these crafts (85.7%). In addition, the results indicate that pottery has promoted experiential rural tourism in the community, thus playing a fundamental role in the daily life of the community's artisan families. This study highlights a little-studied aspect of pottery making, and the results will guide decision-making in the management and promotion of this traditional activity, relating it to other activities such as tourism. Received: 12 July 2024 / Accepted: 31 October 2024 / Published: 05 November 2024

S-nitrosoglutathione reductase disfavors cadmium tolerance in shoots of Arabidopsis

S-nitrosoglutathione reductase (GSNOR) is involved in the response to cadmium (Cd) exposure. In this study, the plants of mutant (gsnor1-3) with lossing-function of- and over-expression (GSNOROE5) of GSNOR were used to clear the role of GSNOR in Cd tolerance. GSNOR activity increased through upregulating the expression of the AtGSNOR gene and protein in Arabidopsis thaliana under Cd stress, which attenuated Cd tolerance. Oxidative damage was more serious in GSNOROE5 and was alleviated in gsnor1-3 under Cd stress, compared with Col-0. Induction of GSNOR facilitated H2O2 accumulation but inhibited catalase (CAT) activity in shoots under Cd stress. This phenotype was eliminated by 3-amino-1,2,4-triazole (3-AT), a CAT inhibitor. In addition, the expressions of AtCAT1 and AtCAT2 were down-regulated with increasing GSNOR activity under Cd stress. This suggested that GSNOR was involved in the accumulation of hydrogen peroxide (H2O2) through regulating CAT expression and activity under Cd exposure. Furthermore, Cd tolerance and CAT activity were improved by spraying S-nitrosoglutathione (GSNO) onto the surface of the leaves. The in vitro activity of CAT increased with GSNO concentration until a GSNO/CAT ratio of 2 was reached. Thus, CAT activity was relative to GSNOR through regulating the expression and S-nitrosylation level of proteins. In summary, the Cd-induced promotion of GSNOR activity aggravated Cd toxicity in plants by mediating H2O2 accumulation controlled by CAT.

A dehydration membrane reactor towards highly efficient LPG synthesis via CO2 hydrogenation

In recent years, CO2 hydrogenation has garnered increasing attention as a promising avenue towards the conversion of captured CO2 to valuable liquid chemicals, including CH3OH, CH3OCH3, and liquified petroleum gas (LPG). However, chemical conversion of CO2 has proven to be immensely challenging, resulting in low CO2 conversion (<35 %) and product yields (<15 %). H2O, unavoidably evolved during CO2 hydrogenation, imposes both thermodynamic and kinetic penalties on CO2 hydrogenation reactions via adsorption on catalyst active sites and promotion of undesirable reaction pathways. In this work, to greatly facilitate direct conversion of CO2 to LPG over a bifunctional Cu/Zn/Zr on Al2O3 (CZZA)/Pd-β-zeolite catalyst, a dehydration membrane reactor (MR) featuring a H2O-conducting Na+-gated nanochannel membrane was studied. Before the incorporation of the Na+-gated membrane, the CZZA/Pd-β-zeolite was unable to produce LPG at 280–310 °C and 14 bar, and the CO2 conversion was limited to < 35 %. In stark contrast, the MR exhibited CO2 conversion and LPG yield of 72.1 % and 51.7 %, respectively. The operating conditions of the MR were systematically investigated to determine the maximum achievable performance resulting in CO2 conversion and LPG yield as high as 90.2 % and 60.5 %, respectively. Furthermore, the LPG space–time yield (STY) at the optimized conditions was near that of the best-performing traditional reactors (TRs) in the literature but at 3 times and 8 times increased CO2 conversion and time factor (W/F), respectively. The MR in this work demonstrates the synergistic efficacy of combined H2O removal and CH3OH consumption towards highly efficient hydrogenation of CO2 for LPG production.

Toward a whole-of-virtual school framework for promoting student physical activity: a scoping review protocol

BackgroundThe advent of full-time virtual schooling presents unique challenges and opportunities for the promotion of physical activity (PA) among children and adolescents. Despite the recognized benefits of PA as an essential component for combating non-communicable diseases and ensuring holistic development, there is a notable gap in understanding how to effectively integrate PA within the digital learning environments of full-time virtual schools. Current efforts to promote student PA are targeted for implementation exclusively in contexts characterized by physical school campuses that are bound to their surrounding local communities. This is problematic given the digital, widely distributed, and contextually unmoored nature of virtual schooling. Our aim in this scoping review is to advance research on whole-of-school physical activity promotion within full-time virtual schools by examining the published literature on whole-of-school PA promotion within full-time virtual schools. Specifically, this review will map the literature, consolidate knowledge claims and practical implications, and identify evidence gaps that merit further investigation.Methods/designThis review will be conducted using evidence-informed scoping review methodology and reporting guidelines. Articles will be included if they are peer-reviewed English-language research, commentary, practical, or grey literature and relate to the participation, support, design, development, and/or provision of remote online PA interventions delivered through primary/elementary and/or secondary/middle school/high schools. Searches will be conducted in PsycInfo, ERIC, SportDiscus, and Web of Science. Additional hand-searching, reference scans, and grey literature searches will also be performed. Two trained research assistants will independently complete study screening and selection and data charting with guidance from a senior author. Charted data will be displayed in table form, and depending on the results, data will also be synthesized through qualitative content analysis using the Active Schools guiding framework as an analytical and interpretive lens.DiscussionThis scoping review will serve as a guidepost for the application and advancement of research on whole-of-school PA promotion through full-time virtual schools. The results will address the increased importance of equitable online learning and PA promotion due to the expanding virtual education landscape, with implications for public health and education policy.Systematic review registrationOpen Science Framework: https://osf.io/f6wau/.

THE ROLE OF NURSING IN THE PREVENTION AND CONTROL OF CHILDHOOD OBESITY

Research on nursing interventions in the prevention and control of childhood obesity has addressed three main determinants: sedentary lifestyles, poor diet, and psychosocial and environmental factors. Through an integrative literature review, negative points associated with each determinant were identified, such as increased risk of obesity, nutritional deficiencies, social stigma, and lack of emotional support. However, several nursing interventions have been highlighted as effective in addressing these problems, including nutritional education, promotion of physical activity, psychosocial support, and advocacy for healthy environments. Authors such as Jardim (2017), Borfe et al. (2017), Serra et al. (2018), among others, have contributed significantly to the development of these strategies. The research highlights the role of nurses in promoting child health, highlighting the importance of a multidimensional and collaborative approach to prevent and control childhood obesity. In summary, the results obtained provide valuable insights to improve nursing practices, promoting healthy lifestyle habits, and combating the childhood obesity epidemic.

Design of urban spaces and connections with sports

This paper aims to investigate the connections between sports and Urban Regeneration, and the role these connections can have in the communities of the cities. The promotion of physical activity within urban areas is today a driving force for economic and social development, and intervenes in the fragile conditions of cities. Design, as a discipline of connection between the active components of these transformations, becomes an instrument of inclusion. The interaction between people and urban space is central in promoting physical activity and a diversified community commitment. The paper investigates practices and tools of the discipline in the relationship between urban spaces and sports practices, and how this relationship leads communities to conceive new development opportunities.

Targeting the G-quadruplex structure in the hTERT promoter for telomerase activity: in silico screening of phyto-compounds and long timescale replica exchange molecular dynamic simulations

Targeting the G-quadruplex structure in the hTERT promoter for telomerase activity: in silico screening of phyto-compounds and long timescale replica exchange molecular dynamic simulations

Global coupling of R-loopdynamics with RNA polymerase II modulates gene expression and early development of Drosophila.

R-loops are involved in many biological processes in cells, yet the regulatory principles for R-loops in vivo and their impact on development remain to be explored. Here, we modified the CUT&Tag strategy to profile R-loops in Drosophila at multiple developmental stages. While high GC content promotes R-loop formation in mammalian cells, it is not required in Drosophila. In contrast, RNAPII abundance appears to be a universal inducing factor for R-loop formation, including active promoters and enhancers, and H3K27me3 decorated repressive regions and intergenic repeat sequences. Importantly, such a regulatory relationship is dynamically maintained throughout development, and development-related transcription factors may regulate RNAPII activation and R-loop dynamics. By ablating Spt6, we further showed the global R-loop induction coupled with RNAPII pausing. Importantly, depending on the gene length, genes underwent up- or down-regulation, both of which were largely reversed by rnh1 overexpression, suggesting that R-loops play a significant role in the divergent regulation of transcription by Spt6 ablation. DNA damage, defects in survival, and cuticle development were similarly alleviated by rnh1 overexpression. Altogether, our findings indicate that dynamic R-loop regulation is dictated by RNAPII pausing and transcription activity, and plays a feedback role in gene regulation, genome stability maintenance, and Drosophila development.

Culturally-relevant physical activity for public health promotion: global evidence synthesis

Abstract Background The WHO has noted the importance of cultural insights for health. Culturally-relevant physical activity has a growing evidence base in many regions and specific populations, but the scope and scale for global health promotion is underexplored. Methods A multinational expert team used a critical literature review method to: (1) define culturally-relevant physical activity; (2) delineate relevant characteristics supporting inclusion in population-level health promotion and surveillance; and (3) provide agenda setting in research, policy, and practice from a global public health promotion perspective. The critical review method, which evaluates rather than summarizes literature, was chosen as the research evidence is complex, broad, and has not been synthesized previously from this perspective. Results Culturally-relevant physical activity was defined as practices that include physical activity based on a population’s cultural customs. These can be Indigenous (e.g., hula, an Indigenous dance of Native Hawaiians) or regional (e.g., Shota (Shotë), an Albanian folk dance). Practices have shared characteristics that support multi-dimensional value for health, engagement, and healing. These include a collectivist, intergenerational component, passed from elders and shared forward with children as part of a community. Many have foundations in nature, a strength for health and wellbeing in green and blue spaces. Many include traditional art, clothing, accessories, and music with deep significance, including care for the tools and location of practice (e.g, drums, boats, land). Many practices would not be captured in harmonized public health surveillance systems despite their value. From this synthesis, research hypotheses were created and next steps proposed. Conclusions This evidence synthesis supports engagement, planning, uptake, policy, surveillance, and study of culturally-relevant physical activity for public health promotion locally and globally. Key messages • An important strength of culturally-relevant physical activity is that it is context and population specific. However, this may hide the scope and scale of the existing evidence supporting this work. • This evidence synthesis confirms how culturally-relevant physical activity is valuable globally across shared assets in research and practice, supporting next steps for public health promotion.

Assessment of controlled assistance from e-bikes as a mitigation strategy for individual PM exposure

Abstract Background While promoting active mobility and outdoor activities can benefit health (HLT), it also increases Exposure (EXP) to air pollutants, such as particulate matter (PM), due to the increased ventilation rate (VR) under physical activity. Traditional assessments of individual (IND) PM EXP involve costly equipment inaccessible to the public, but real-time low-cost sensors (LCS) are now emerging. This study presents a proof of concept to demonstrate the use of LCS for examining the real-time IND respiratory dose deposited (RDD) to PM cycling across the Brescia province. Methods An e-bike was ridden 33 times between 2022 and 2023 wearing a LCS for PM concentration [PM] linked to a GPS. Respiratory dose deposited(RDD) was calculated for six different locations within each route based on experimental PM and various VR. Results The LCS allows for the study of the spatial and temporal variability of [PM], revealing a significant difference (p &amp;lt; 0.01) according to the heating system operation and no significant difference (p &amp;gt; 0.05) depending on the location. RDD increases with [PM] and VR. Real-time and integrated RDD is calculated for routes with high (&amp;gt; 50 µg/m3), medium (25 µg/m3), and low (&amp;lt;10 µg/m3) [PM2.5]. The e-bike assistance is activated when the WHO interim target 4 of [PM] is reached to reduce VR, which corresponds to a shift to a lower physical activity intensity. The RDD calculated using the e-bike assistance over the path reduces RDD by about 23% and 11% over the high and medium [PM], while it never activates for the low [PM]. Conclusions This study demonstrates that the use of LCS allows the estimation of IND RDD, and the implementation of mitigation strategies based on controlled assistance from e-bikes would enable the reduction of PM individual EXP. Key messages • The integration of HLT data from wearable and air quality LCS enables the promotion of active mobility and outdoor activities controlling the EXP to air pollutants. • Establishing generalized guidelines based on this mitigation strategies for individual exposure reduction may also inform policy recommendations for promoting active and green mobility.

Urban transformation interventions for the promotion of active home-school mobility: overview

Abstract Background Active home-school mobility can contribute to a series of benefits, both for the health of children and adolescents (higher levels of physical activity, better cardio-metabolic profile, better school performance), and for the health of the general population and of environment (reduction of air pollution and greenhouse gas emissions). An overview of systematic reviews (RS) was conducted in order to evaluate mainly the effectiveness of interventions promoting active home-school mobility on physical activity, overweight/obesity, well-being and reduction of road accidents in children and adolescents. Methods The bibliographic search was carried out on the following databases: Cochrane Database of Systematic Reviews, MEDLINE, Embase and CINAHL, without any limits. Endnote software was used for the systematic reviews (SRs) selection. Data relating to the main characteristics and results of the SRs were extracted. The assessment of the methodological quality of the SRs was carried out using the “Health Evidence™ Quality Assessment Tool”. Results On the basis of the relevance and adherence to the research PICO, n. 12 systematic literature reviews evaluating effects of interventions of interest in the study population. The strategies took multiple levels into consideration, i.e. they aimed at organizing the home-school journey to encourage active mobility, as well as training and information aimed at the population under study for the promotion of physical activity. Conclusions Combined interventions appear more effective in promoting active home-school mobility with a better impact on increasing physical activity levels. Further high quality studies are necessary to investigate the impact of these interventions also on the reduction of incidence of overweight/obesity and in terms of school performance. Key messages • Policy makers should promote programs to increase active home-school transport. • Further high quality studies are necessary to investigate the impact of these interventions also on the reduction of incidence of overweight/obesity and in terms of school performance.

Physical activity promotion in Aged care: a participatory approach

Abstract Introduction Aged care facilities (ACF) constitute an increasingly important, yet challenging setting for health promotion - given it is a highly sedentary pseudo-total institution characterized by structured daily routines within a paradigm of care for residents perceived as fragile and vulnerable. A participatory approach with residents, significant others, staff, and management might be a promising pathway to address intrapersonal, sociocultural, organizational, environmental, and political determinants of physical activity in this specific context. We will present processes and results from a research project, funded by the German Ministry of Health (2019-2023), on physical activity promotion in ACFs. Methods Our co-creation approach involved residents, significant others, staff, and management from seven nursing homes in Southern Germany. Methods included 800hrs on-site observations, semi-structured interviews (n = 31), Photovoice (n = 27 participants, n = 169 photos), as well as future workshops (n = 14) for intervention co-development (n = 14) and co-evaluation workshops (n = 7). Data analysis used reflexive thematic analysis with MaxQDA20. Results Implemented interventions (n = 57) addressed individual, social, environmental, and organizational factors. Activity promotion was integrated into care concepts and mission statements, staff work descriptions, as well as informal internal routines. Of the interventions implemented, 33% were rated as more successful, 43% as successful, and 19% as less successful than anticipated. Conclusions Participation can initiate structural and cultural change towards physical activity. However, the analyses also emphasized the critical role of staff training, along with the involvement of significant others and external service providers, as essential components for achieving sustainable change. Key messages • Participatory approaches integrate physical activity into aged care, fostering cultural shifts for resident well-being. • Success requires staff training, engaging residents’ support networks, balancing empowerment with protection.

Artificial sport surfaces and adverse effects for human health. A literature review

Abstract The management of the Post-Consumer Tyres (PCTs) is still a hot topic. In 2021, the 27 EU Member States plus Norway, Switzerland, and the UK discarded 4,200 Ktons of PCTs. The main application of the rubber granules obtained from their recycling is represented by infill material in synthetic turf playgrounds. These widespread installations have become fundamental tools for the promotion of physical activity, especially among children. However, this material is in the spotlight for the presence in its composition of hazardous substances and for the small size of granules, which the European Commission classified as “microplastics”. This review aims to clarify the scientific knowledge, focusing on the assessment of the bio-accessibility/availability of hazardous chemicals and the risk assessment for users. Eligible articles reporting original data were identified in CAB Direct, Embase, PubMed, Scopus, and Web of Science. The included articles (preliminary results) variously demonstrated the presence of PAHs, metals, volatile organic compounds, and phthalates in artificial biofluids (n = 9 articles). The risk assessment for these toxicants and the potential cancer risk induction (n = 19), revealed a risk for users from absent or negligible to potential, according to the exposure scenario considered and the relative assumptions, the contaminants, the exposure route, the age of the pitch, and characteristics of the rubber granules such as origin, concentration of the different toxicants, and particle size. The assessment of the potential hazard due to the exposure to artificial sports surfaces is anything but simple. Indeed, the crumb rubber compositions may vary in different settings and over time and can be variously influenced by the climate and environmental conditions, making it difficult to quantify the possible health effects, as well as the specific mixture, the quantities, and sources responsible for them (Funded by LIFE20 GIE FR 282 - RE-PLAN CITY LIFE). Key messages • The bioccessibility of PAHs, metals, volatile organic compounds, and phthalates included in the infill material composition was variously demonstrated in literature. • The assessment of the potential risks due to the exposure to synthetic turf, revealed a risk from negligible to potential, according to the exposure scenario considered and the relative assumptions.

Association of physical activity with health-related quality of life in rectal cancer survivors

Abstract Background Health-related quality of life (HRQoL) in rectal cancer survivors after completion of surgical treatment may be influenced by various factors including physical activity. The purpose of this study was to examine the association between regular exercise and physical and mental health in rectal cancer survivors. Methods People who underwent surgical resection of rectal cancer with temporary ileostomy from 2018 to 2021 were invited to participate. Participants were drawn from the Clinic for Digestive Surgery and the Clinic for Emergency Surgery of the University Clinical Center of Serbia, as well as the nationwide laparoscopic program - LapSerb consortium. Data were collected through interviews in late 2022 and early 2023. The HRQoL was evaluated using the physical composite score (PCS) and mental composite score (MCS) of the Short Form-36 (SF-36) questionnaire. Socio-demographic, clinical and behavioral characteristics including the frequency of exercise were analyzed in multiple linear regression models. Results A total of 134 rectal cancer survivors were tested. Few participants (18.7%) exercised regularly, while most of the study population occasionally (41.8%) or never engaged (39.5%) in physical activity. Rectal cancer survivors who exercised regularly had significantly higher scores of physical (75.5 vs. 62.8, p = 0.019) and mental quality of life (78.9 vs. 63.8, p = 0.008). Regular exercise remained associated with better physical and mental health after the adjustment for demographic, clinical and behavioral characteristics. Conclusions Consistent physical exercise seems to be significant contributor of better physical and mental quality of life in rectal cancer survivors after completion of surgical treatment. Key messages • Physical exercise may help rectal cancer survivors improve their physical and mental health. • Promotion of physcial activity in rectal cancer survivors could optimize recovery after surgery.

Physical activity promotion as intersectoral planning in Milan Health Protection Agency

Abstract Issue/problem Physical activity is a protective factor for preventing Noncommunicable diseases (NCDs) and, for this reason, it plays an important role in all the different guidelines for prevention and health promotion. The Italian Regional Prevention Plans and in particular the Lombardy one promote physical activity with a systemic approach, through both upstream and downstream actions to encourage the role of communities. Description of the problem Based on the regional plan, the Health Protection Agency (HPA) of the Metropolitan City of Milan developed locally validated actions and best practices for the promotion of physical activity, through the cross-sectoral involvement of different stakeholders (health system, companies, universities, local authorities, sports associations, etc.). Results Physical activity is embedded in all lines of health promotion activities in local planning documents. Sustainable and active mobility activities have been implemented in companies and schools with a comprehensive approach. Validated regional programs (Pedibus, Walking Groups, ecc.) have been implemented in the local community, along with the construction of permanent local laboratories for physical activity, structured through the development of specific territorial networks and providing for integration with the socio-sanitary setting. Lessons Promoting physical activity becomes an opportunity to both consolidate territorial networks and to develop evidence-based health promotion actions able to meet the different health needs of the population and to produce a structural change in living contexts. Key messages • Physical activity is both a protective factor for health promotion and a tool for implementing intersectoral and equity-oriented system actions from a life-course perspective. • Promoting physical activity becomes an opportunity to consolidate territorial networks.

Cardiomyocyte proliferation is promoted by intracellular Gas6-mediated Yap activity during neonatal heart regeneration

Abstract Purpose The adult mammalian heart has limited regenerative capacity after injury, mostly attributable to postnatal cardiomyocyte (CM) cell cycle arrest. The key to unlocking the regenerative potential of the adult hearts may lie within the developmental transitions occurring during neonatal life. Gas6, a secretory protein, stimulates the proliferation of various type cells through binding to TAM receptor family, such as endothelial progenitor cells, tumor cells, and hair follicle stem-cell. However, no study to date has studied the role of Gas6 during the proliferation of CMs. Herein, our study uncovers that Gas6 is transiently expressed during early postnatal heart and regenerative heart, and functions through intracellular pathway by regulating Yap activity to promote CM proliferation. Methods CM isolation was conducted to assess the change of Gas6 expression in both postnatal development and regenerated stages using the CM-specific Tdtomato mice. Cardiac deletion of Gas6 gene mice was used to evaluate the impaired heart regeneration. CM-specific Gas6 overexpression mice were generated using adeno-associated virus system to evaluate Gas6 pro-proliferate and protect effect after ischemic injury. Secreted signal peptide truncation (ΔSP-Gas6) and predicted functional region truncation (ΔCC-Gas6) were generated to identify the intracellular mechanism of Gas6 via Sav1/p-mst1/p-lats1/ p-yap axis. Results We found that Gas6 expression is enriched in CM4 and the expression profile correlates with the expression of the CM4 marker Acta2, as well as cell-cycle genes Mki67 and Aurora B, in individual CM. Gas6 was highly expressed in the neonatal heart and continued to increase during the neonatal heart regenerative process, but was nearly absent in the adult heart under physiological and ischemia conditions. CM-specific Gas6 deletion resulted in significantly reduced CM proliferation rate and increased CM size in P0 hearts, and congestive heart failure in P28 hearts. Conversely, sustained CM-specific overexpression of Gas6 in postnatal hearts increased CM division, elevated CM numbers, and reduced CM size; and reduced infarct scar area and enhanced cardiac function after ischemia injury. Pre-overexpression of Gas6 in adult CMs protected adult cardiac function against ischemia injury, indicated by the activation of mitosis, angiogenesis and reduced oxidative phosphorylation. In primary neonatal CMs, both Gas6 and ΔSP-Gas6promoted CM proliferation with sarcomere disassembly. Mechanistically, Gas6 interacted with Sav1to inhibit Mst1recruitment and Mst1/Lats1/Yap phosphorylation cascade, therefore, promoted Yap translocation into nucleus. Conclusions We demonstrate that Gas6 is an important intrinsic regulator of neonatal heart regeneration, which acts as a promoter of Yap transcriptional activity to enhance CM proliferation. Supplementing Gas6 in the adult heart represents a potential therapeutic approach for cardiac repair.

Reducing obesity and overweight: Subregional Policy Dialogues in the WHO European Region

Abstract In the WHO European Region, obesity and overweight stand as leading causes of disability and premature mortality. They contribute significantly to the burden of noncommunicable diseases, hindering efforts to achieve sustainable development goals related to health, including SDG goal 3.4, aiming to reduce premature mortality from NCDs by one-third by 2030. Despite some policy successes such as sugary drinks taxes, restrictions on marketing unhealthy products to children, and physical activity promotion campaigns, no country has managed to halt the rising levels of obesity. Addressing obesity requires a comprehensive approach, and WHO endeavors to support countries in implementing a wide range of policy actions across various settings. The WHO Special Initiative for NCDs and Innovation has recently adopted a subregional approach to promote evidence-based, data-informed policy action. In June 2022, it organized a series of obesity dialogues, including sessions for the Western Balkans. This session will outline the methods and outcomes of these dialogues, including stakeholder engagement, presentation of the latest obesity data, evidence-based policy recommendations, and the process of prioritizing policy options with decision-makers. It will consider the lessons learned from this process, offering insights not only for the health agenda but also for addressing other complex public health, environmental, or social challenges, which require multisectoral collaboration and comprehensive, policy actions on many levels.