Activity Of Phenolic Compounds Research Articles

Effect of curcumin on regulatory B cells in chronic colitis mice involving TLR/MyD88 signaling pathway.

Curcumin (Cur) is a natural active phenolic compound extracted from the root of Curcuma Longa L. It has anti-inflammatory, anti-tumor and other pharmacological activities, and is commonly used to treat ulcerative colitis (UC). However, it is not clear whether curcumin regulates the function and differentiation of Breg cells to treat UC. In this study, mice with chronic colitis were induced by dextran sulfate sodium (DSS), and treated with curcumin for 12 days. Curcumin effectively improved the body weight, colonic weight, colonic length, decreased colonic weight index and pathological injury score under colonoscopy in mice with chronic colitis, and significantly inhibited the production of IL-1β, IL-6, IL-33, CCL-2, IFN-γ, TNF-α, and promoted the secretion of IL-4, IL-10, IL-13 and IgA. Importantly, curcumin markedly upregulated CD3- CD19+ CD1d+ , CD3- CD19+ CD25+ , CD3- CD19+ Foxp3+ Breg cells level and significantly down-regulated CD3- CD19+ PD-L1+ , CD3- CD19+ tim-1+ , CD3- CD19+ CD27+ Breg cells level. In addition, our results also showed that curcumin observably inhibited TLR2, TLR4, TLR5, MyD88, IRAK4, p-IRAK4, NF-κB P65, IRAK1, TRAF6, TAB1, TAB2, TAK1, MKK3, MKK6, p38MAPK, p-p38MAPK and CREB expression in TLR/MyD88 signaling pathway. These results suggest that curcumin can regulate the differentiation and function of Breg cell to alleviate DSS-induced colitis, which may be realized by inhibiting TLR/MyD88 pathway.

Changes in phenolic compounds and antioxidant activities of “nine steaming nine sun-drying” black soybeans before and after in vitro simulated gastrointestinal digestion

Black soybean (Glycine max (L.) Merr.) is rich in phenolic compounds, and processing technology has a significant effect on the content and activity of phenolic compounds. However, the mechanism of nine steaming and nine sun-drying processing technique is not fully understood. This paper presents the changes of phenolics content, phenolic acids composition and their influence on antioxidant activity before and after in vitro simulated gastrointestinal digestion of black soybeans (BS) under the process of nine steaming nine sun-drying. Results showed that the total phenolic content (TPC) and total flavonoids content (TFC) in BS were reduced by the heat treatment method, and exhibited a decreasing trend with more steaming and sun-drying cycle. During in vitro digestion, the contents and bioaccessibility of 12 phenolic acids (PA-12) in BS were highest in the stomach, followed by mouth and the intestine. The bioaccessibility of PA-12 in steamed and sun-dried BS was higher than that of raw black soybeans (S0D0) after digestion. It reached maximum after digestion at the third steaming and sun-drying cycle (i.e. S3D3), wherein the phenolic acids with the highest bioaccessibility were syringic acid, gallic acid, ferulic acid and chlorogenic acid. Syringic acid, in particular, increased significantly during digestion compared with that before digestion, which also increased during processing. The antioxidant activity of in vitro digested BS products with appropriate steaming and sun-drying degree increased compared with S0D0. Principal component analysis (PCA) showed that the in vitro digestion-induced properties of steamed and sun-dried BS could be well distinguished. The results confirm that the phenolic compounds and bioaccessibility of nine steamed nine sun-dried BS must be taken into account when assessing the improvement of human health.

Development of Functional Kiwifruit Jelly with chenpi (FKJ) by 3D Food Printing Technology and Its Anti-Obesity and Antioxidant Potentials.

With the growing popularity of the concept of healthy diet, modern obesity treatment is gradually shifting from surgical or pharmacological treatment to nutritional intervention. As a safe and effective measure, natural product interventions are a potential strategy of obesity management. The present study aimed to develop a kind of functional food rich in bioactive compounds (chenpi, kiwifruit, and pectin as raw materials) and investigate their bioactive effects on a mouse model. For development of functional kiwifruit jelly with chenpi (FKJ), the results of single-factor and response surface experiments showed that the optimized formulation was composed of a 30.26% addition of chenpi, 35% addition of kiwifruit juice, and 2.88% addition of pectin. The FKJ obtained with the optimal formulation could be used as a 3D printing raw material to print the desired food shapes successfully. For bioactivity evaluation of FKJ, the results with a mouse model showed that the food intake, liver weight, and adipose tissue weight were significantly decreased after administration of FKJ with dose-dependent effect compared to the CON group (p < 0.05). Meanwhile, the serum levels of several inflammatory factors (TG, IL-6, and TNF-α) were decreased and the activities of several antioxidant-related enzymes (SOD, GSH-PX, and CAT) were increased. In short, a functional kiwifruit jelly with chenpi was developed in this study. It is a functional snack food rich in active phenolic compounds, low in calories, with antioxidant and anti-inflammatory activity, and prevents fat accumulation. FKJ could well meet the needs of modern people for nutrition and health and also promote the processing and utilization of natural products, and has good development prospects in the functional food industry.

First Report on Bioaccessibility, Antioxidant Activity and Total Phenolic Compounds From Stachys thirkei C.Koch Using A Simulated In Vitro Digestion System

It is of great importance to determine the antioxidant properties of plants, especially those used for food, pharmacology and medicinal purposes. Stachys thirkei C.Koch belonging to Lamiaceae family is used as a medicinal aromatic plant in Turkey. The present study was carried out to investigate the total phenolic contents, antioxidant activity and bioaccessibility of S. thirkei C.Koch. The total phenolic content (TPC) was evaluated by Folin-Ciocalteu colorimetric procedure and antioxidant activity determine four distinctive methods (ABTS•+, CUPRAC, DPPH• and FRAP). According to the results, the levels of hydrolysable phenolics (1538.99±4.57 mg of GAE/100g) approximately three and a half fold higher than extractable phenolics (422.96±4.70 mg of GAE/100g). The TPC of S. thirkei C.Koch was determined to be 1961.95±4.46 mg of GAE/100g. On the other hand, the bioaccessible fractions and phenolic bioaccessibility of S. thirkei C.Koch were found to be 1766.72±2.96 μmol Trolox/g and 90.05±1.50%, respectively. At the same time, the antioxidative bioaccessibility of S. thirkei was found to be higher in FRAP method (1164.29 μmol Trolox/g) and also the bioaccessibility (%) of S. thirkei C.Koch was found to be higher in CUPRAC method (93.41%). Present study is the first report to investigate the antioxidant effect and bioaccessibility of the extracts from S. thirkei C.Koch. The results of the present study can be strong scientific proof to use this plant as a beneficial source of antioxidant referments and the studies can be further extended to investigate whether they exhibit similar activities in in vivo systems.

GC MS Based Comparative Phytochemical Profiling of Rhodiola Imbricata Roots Collected from Different High Mountain Passes of Ladakh India and a First Report of Apocynin from Genus Rhodiola

Rhodiola imbricata is a high value medicinal plant of trans-Himalayan mountain passes in Ladakh. This plant is a highly sought after in national and international herbal product market due to its unique phytochemical composition and resultant medicinal properties. However, compositional variation in the raw material from different geographical locations results in variation in quality as well as efficacy of the final products. The current study was designed to generate the comparative GC-MS profiles of hydro-methanolic extracts of Rhodiola imbricata root samples collected from various locations in Ladakh i.e., Chang La (17605 ft), Khardung La (18,379 ft), and Shashi La (13908 ft) mountain passes. The study highlighted variations in volatile phytochemical composition in root samples collected from different locations, especially with respect to phenols, terpenes and fatty acids. Samples from Chang La had maximum amount of phenolic compounds (96.78 per cent), followed by samples from Khardung La (77.05 per cent) while they were undetected in samples from Shashi La pass. Specifically, comparative GC-MS profiling revealed that peak area percentage of two important bioactive compounds (i.e. piceol and apocynin) varied amongst samples. In the samples collected from Chang La, the piceol covered (94 per cent) and apocynin covered (2.78 per cent) peak area whereas in samples collected from Khardung La, piceol covered (73.8 per cent) and apocynin covered (3.25 per cent) peak areas respectively, however, samples collected from Shashi La showed none of these compounds. Interestingly, in the present study, apocynin (a potent NADPH oxidase inhibitor) is being reported for the first time from Rhodoila genus. On the basis of present findings, samples collected from Chang La and Khardung La passes showed better phytochemical composition of pharmacological active phenolic compounds than Shashi La sample.

Antimicrobial Screening and Fungicidal Properties of Eucalýptus globulus Ultrasonic Extracts.

The prohibition of antibiotics has led to extensive research and use of phytogenic feed additives. James Barrie Kirkpatrick described four subspecies of eucalyptus (family Myrtaceae), including Eucalýptus globulus, in 1974. The maximum concentrations of quercetin-3D-glycoside (1703.30 g/mL), astragalin (1737.82 g/mL), chlorogenic acid (342.14 g/mL), catechin (282.54 g/mL), rosmarinic acid (36.39 g/mL), and 3,4-dihydroxybenzoic acid (27.55 g/mL) were found in samples of ultrasonic extraction with ethyl alcohol (extraction module 1:5, temperature of 32 °C, an ultrasonic exposure time of 25 min). Antimicrobial activity was observed in all studied samples after 12 h of incubation (against gram-positive (Bacillus subtilis) and gram-negative (Pseudomonas aeruginosa) bacteria, as well as representatives of yeast fungi (Candida albicans)); a more pronounced antimicrobial effect (lysis zone) was observed after ultrasonic processing of extracts for 20 and 25 min. Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans had lysis areas of 10.0 mm (20 min extraction with ultrasonic treatment), 13.0 mm (20 min extraction without ultrasonic treatment), and 15.5 mm (25 min extraction with ultrasonic treatment), respectively. E. globulus was demonstrated to be a source of biologically active phenolic compounds with antibacterial and fungicidal activity. More research on the use of E. globulus in feed additives is required.

Yellow Mombin Nectar Enriched with Encapsulated Green Tea (Camellia Sinensis Var Assamica): Physical-chemical, Rheological and Sensory Aspects

ABSTRACT This research aimed to evaluate the feasibility of applying green tea extract encapsulated in yellow mombin (Spondias mombin L.) nectar. Two formulations were developed: with microencapsulated green tea extract (NM) and control (NC). NM was the variant concentration of green tea microencapsulated (4.6 g of green tea microcapsules for each 240 mL of beverage). The samples were subjected to physical-chemical and bioactive compounds analysis, such as the study of rheological behavior and sensory analysis. The addition of microcapsules resulted in changes in all of the parameters evaluated, highlighting the contribution in antioxidant activity and total phenolic compounds. The inclusion of microcapsules did not influence the rheological behavior. For the sensory analysis, it was found that NC presented better sensory acceptance. The inclusion of microcapsules of green tea extract in yellow mombin nectar presents itself as a viable alternative for the development of a new product.

QSAR study of phenolic compounds and their anti-DPPH radical activity by discriminant analysis

Phenolic compounds (PCs) could be applied to reduce reactive oxygen species (ROS) levels, and are used to prevent and treat diseases related to oxidative stress. QSAR study was applied to elucidate the relationship between the molecular descriptors and physicochemical properties of polyphenol analogues and their DPPH radical scavenging capability, to guide the design and discovery of highly-potent antioxidant substances more efficiently. PubMed database was used to collect 99 PCs with antioxidant activity, whereas, 105 negative PCs were found in ChEMBL database; their molecular descriptors were generated with Python's Rdkit package. While the molecular descriptors significantly related to the antioxidant activity of PCs were filtered by t-test. The prediction QSAR model was then established by discriminant analysis, and the obtained model was verified by the back-substitution and Leave-One-Out cross-validation methods along with heat map. It was revealed that the anti-DPPH radical activity of PCs was correlated with the drug-likeness and molecular fingerprints, physicochemical, topological, constitutional and electronic property. The established QSAR model could explicitly predict the antioxidant activity of polyphenols, thus were applicable to evaluate the potential of candidates as antioxidants.

Biochemical Characterization of Six Traditional Olive Cultivars: A Comparative Study

Olives are an essential ingredient in Turkish food culture. Turkey has grown to become one of the top five producers of olives and olive oils in the world. Olive trees in general are found in the coastal parts of the country. The fruits of six traditional cultivars of olive (Gemlik, Domat, Memecik, Ayvalik, Cilli, and Adana Topagi), grown in Adana, were characterized based on their fruit skin color, the amount of chlorophylls, fatty acids, antioxidant activity, and total phenolic compounds, as well as volatile compounds. The international cultivar Manzanilla, grown in the same orchards as the traditional cultivars, was also included in the study to make a comparison. Compared to the Manzanilla cultivar, Memecik showed the highest amount of total phenolic content and antioxidant activity with the highest level of lightness, blueness/yellowness, and color intensity. Ayvalik presented the highest level of greenness/redness. Although the highest fruit lightness and darkness were found in the Cilli cultivar, with the highest amount of chlorophyll a (2.63 mgL−1), there was more chlorophyll b in Adana Topagi fruits (3.34 mgL−1). The highest percentage of fatty acids was found in the Gemlik cultivar of 66.81%, among which oleic acid was the major component. The total aldehydes ranged between 33.43% and 50.60%. Compared to Manzanilla and the traditional cultivars, the Domat cultivar had the highest amount of hexanal of 44.42%. Adana Topagi had the highest amount of alcohols (61.34%) and acids (1.31%). Memecik registered the highest amount of ketones (17.86%) and terpenes (20.34%). Among all cultivars, Manzanilla displayed the highest amount of esters (2.30%). In this study, traditional cultivars were found to be the richest in health-promoting chemicals. Furthermore, a significant variability among the cultivars was revealed, implying that metabolic fingerprinting approaches could be used to differentiate cultivars once more research into the effects of the growing conditions and environmental factors on the chemical profiles of each cultivar is carried out.

Hydnopolyporus fimbriatus (Fr.) D.A. Reid mushroom: phytochemical screening, antioxidant activity, total flavonoid and total phenolic compounds

Hydnopolyporus fimbriatus is a species of mushroom of the Meripilaceae Jülich family found in some regions of Central and South America. This study aimed to evaluate the qualitative phytochemical composition, the antioxidant activity in the reduction of DPPH and hydroxyl free radicals and to determine the flavonoid contents and total phenolics in the ethanolic extract of the mushroom H. fimbriatus. The ethanolic extract was prepared and lyophilized. Qualitative phytochemical prospection assays were performed for different groups of organic compounds. The antioxidant assay was carried out in the reduction of the 2,2-diphenyl-1-picrylhy-drazyl free radical and in the reduction of the hydroxyl free radical by colorimetric methods. The ethanolic extract of H. fimbriatus positively exhibited several phytochemical groups, moderate antioxidant activity in reducing free radical DPPH 101.09 µ mL-1, high hydroxyl radical reducing activity 87.51% and potential total phenolic content 20.15 mg GAE 100 g-1 of dry extract. Flavonoids content was not observed possibly due to the colorimetric method using aluminum chloride. With these results, it was possible to characterize the ethanolic extract of the vegetative part of Hydnopolyporus fimbriatus. New studies should be carried out in an attempt to develop a quantitative method of total flavonoids as well as test the extract in other biological models, thus being able to develop new drugs or products for agricultural use.

NUTRIENTS CONTENT IN FRUITS OF VARIETIES AND HYBRIDS OF GUELDER ROSE (VIBURNUM OPULUS L.) IN CONDITIONS OF THE WESTERN SIBERIAN SOUTH

Guelder rose (V. opulus L., 1753) is a rare garden crop widespread throughout Russia. It is a valuable food and medicinal raw material due to its rich chemical composition. It contains biologically active phenolic compounds, pectin substances, triterpenoids, polysaccharides, carbohydrates, organic acids, and macro- and micro-elements.&#x0D; The study aimed to investigate the content and variability of soluble solids (SS) and sugars in fruits of V. opulus L. in the conditions of the South of Western Siberia. The research objects are fruits of V. opulus L. of 2 varieties (Taezhnye Rubiny and Avrora) and 15 hybrids. The authors employ the Taezhnye Rubiny variety as a control.&#x0D; The SS content has been determined by the refractometric method and the mass content of sugars – by direct titration of an aqueous extract. The authors calculate Selyaninov’s hydrothermal coefficient (HTC) to characterize the growing season. The limits are determined for the accumulation of SS (from 10.2% to 18.2%) and sugars (from 7.3% to 10.0%) and the variability of these features (14% and 10%, respectively).&#x0D; The number of genotypes with a stable accumulation of SS identified over the years is 82%, and that with high sugar content (8.6%–10.0%) is 35%. The analysis reveals an average adverse correlation between HTC and the accumulation of SS (r=-0.62) and sugars (r=-0.34) in fruits and a strong dependence of these biochemical features on HTC in control (r=0.93, r=0.95).

The effects of aqueous leaf extract of Portulaca oleracea on haemato-biochemical and histopathological changes induced by sub-chronic aluminium toxicity in male wistar rats

BackgroundPurslane has wide acceptability as a potherb in Central Europe, Asia, and Mediterranean region, it is very used in traditional Chinese medicine (TCM) to treat fever, dysentery, diarrhea, carbuncles, eczema, and to remove toxic substances. Our study aimed to investigate the impact of purslan on physiological and biochemical alterations induced by AlCl3 intoxication in rats. MethodologyFifteen males albino Wistar rats were randomly divided into 3 groups (n = 5); healthy rats (Control), AlCl3 (200 mg/kg b.w) treated rats and AlCl3 (200 mg/kg b.w) rats treated with aqueous extract of P. oleracea (400 mg/kg b.w). AlCl3 treatments were given to rats orally for 70 days and purslan extract for 21 days. Various parameters as hematological, biochemical, oxidative stress markers and liver histopathology were estimated. In-vitro study of plant, some quantitative parameters was analyzed using standard protocols. ResultsIn vitro phytochemical test results revealed that purslane contains most of the principal active molecules, especially flavonoids and phenolic compounds. In vivo results, the AlCl3 group showed an alteration in the antioxidant defend system by increasing the MDA level and decreasing the GSH levels, GST and SOD activities compared to control. Furthermore, the hematological parameters revealed that AlCl3 induce an inflammation in rats by significant raise (P<0.01) of WBC, Monocyte, LYM and platelet (P<0.001) levels with a significant perturbation in lipid profile and biochemical parameters. In the other hand, histopathological analysis recorded a deep modification in liver tissues of AlCl3 rats group compared to control. However, the treatment of AlCl3 rats by purslan ensured a partial amelioration and correction of the previous parameters. ConclusionWe conclude that the use of purslane seems to be the powerful limited of AlCl3 toxicity development, which opening the new horizons for the use of phytotherapy in medical approach.

Quinoa sourdough-based biscuits with high antioxidant activity fermented with autochthonous lactic acid bacteria

To evaluate the capacity of autochthonous lactic acid bacteria (LAB) (43) from Andean grains to increase the antioxidant activity (AOA) and total phenolic compounds (TPCs) in quinoa sourdough to select best performing strains to be used as starter cultures in the elaboration of biscuits. Microbial growth (CFU per g) and pH were evaluated during quinoa dough fermentation. Counts were increased in a range of 0.61-2.97log CFU per g and pH values between 3.95 and 4.54 were determined after 24h at 30°C of fermentation. Methanolic (ME) and aqueous (AE) extracts were obtained at the end of fermentation, and free radical scavenging capacity was performed by the DPPH and ABTS methods. ME was selected for further analysis using other methods and TPC quantification. Principal component analysis showed the highest scores of growth, acidification capacity, AOA and TPC for the strains Lc. mesenteroides subsp. mesenteroides CRL 2131 and L. plantarum CRL 1964 and CRL 1973. AOA and TPC in biscuits made with sourdough from these LAB were higher than the acidified and uninoculated controls. Autochthonous LAB strains (3) increased the AOA of quinoa-based biscuits. Quinoa sourdough obtained with selected LAB is suitable as an ingredient for bakery foods with improved antioxidant status.

Finding the optimum treatment procedure to delay honey crystallization without reducing its quality

The current study investigated the effect of different thermal and non-thermal procedures for the estimation of the optimum conditions of treatment of different honey types (erica, cotton, thyme, eucalyptus and pine honey) to avoid or delay crystallization without losing the nutritional benefits of honey. Different physicochemical parameters, such as pollen analysis, hydroxymethylfurfural (HMF), diastase activity, acidity, and total phenolic compounds were used as quality indicators. The different treatment procedures were evaluated in different honey types and in different time periods. It was proved that to achieve a non-crystallised product that lasts over a year, samples must be heated at 72 oC or stored at −18 °C. However, only by storing samples at −18 °C kept the nutritional benefits of honey and provide similar results with the fresh unheated samples. All procedures were examined in a long-term period (one year) in order to have a complete and accurate result.

Kinetics of the in−situ hydrogenation of phenol with formic acid as a hydrogen source

Highly active phenolic compounds in biomass pyrolysis oil are an important factor for limiting the utilization of the biofuel. The catalytic hydrogenation of phenolic compounds is considered to be an effective method for reforming bio−oil. Pd/CB (carbon black), which was synthesized by using a facile impregnated method, is found to be an effective catalyst for the in-situ hydrogenation of phenol (a representative model compound of bio−oil) using FA as a hydrogen source to produce cyclohexanone. A 98.99% of the conversion of phenol and 90.20% of the selectivity of cyclohexanone were obtained under the optimized reaction conditions. The catalyst also showed excellent stability after three recycled process. The catalytic kinetics study of the in−situ hydrogenation of phenol was investigated using Power−Rate Law model and Langmuir−Hinshelwood model. The Langmuir−Hinshelwood model fit well to the experimental data and the apparent activation energies (Ea) was 50.96 kJ mol−1.

Detailed investigation of the composition and transformations of phenolic compounds in fresh and fermented Vaccinium floribundum berry extracts by high-resolution mass spectrometry and bioinformatics.

IntroductionBlueberries are known for their very high content of biologically active phenolic compounds; nonetheless, differently from the North American and European species of blueberries, Neotropical blueberries have not been extensively studied yet.ObjectivesIn the present paper, the phenolic composition of Vaccinium floribundum Kunth, which is endemic to the Andean regions and grows 1,600 to 4,500 meters above sea level, was investigated by ultra‐high‐performance liquid chromatography coupled to high‐resolution mass spectrometry (UHPLC‐HRMS). Native and fermented berries were compared in terms of phenolic composition as well as antioxidant activity, total phenolic content, and total anthocyanin content.Materials and Methods V. floribundum native and fermented berries were extracted and analyzed by UHPLC‐HRMS. The acquired datasets were processed by Compound Discoverer 3.1 using a dedicated data analysis workflow that was specifically set up for phenolic compound identification.ResultsIn total, 309 compounds were tentatively identified, including anthocyanins, flavonoids, phenolic acids, and proanthocyanidins. The molecular transformations of phenolic compounds during fermentation were comprehensively investigated for the first time, and by a customized data processing workflow, 13 quinones and quinone methides were tentatively identified in the fermented samples. Compared to other species of the genus Vaccinium, a peculiar phenolic profile is observed, with low abundance of highly methylated compounds.ConclusionAndean berries are a rich source of a wide variety of phenolic compounds. Untargeted MS analyses coupled to a dedicated data processing workflow allowed expanding the current knowledge on these berries, improving our understanding of the fate of phenolic compounds after fermentation.

Effects of different extraction on the antibacterial and antioxidant activities of phenolic compounds of areca nut (husks and seeds)

Effects of different extraction on the antibacterial and antioxidant activities of phenolic compounds of areca nut (husks and seeds)

New Method for Obtaining a Bioactive Essence Extracted from Iberian Ham Fat Rich in MUFA and Antioxidants.

Iberian ham is one of the most representative Spanish products and presents an excellent nutritional and sensory quality. Iberian ham trimming fat is considered a by-product and to give a new use to this remaining part could represent a healthy and innovative option for obtaining sustainable foods. The purpose of this work was to obtain a new bioactive ingredient from Iberian ham trimming fat with the highest amount of antioxidants and monounsaturated fatty acids (MUFA), using a new non-invasive solvent-free method. To obtain the essence, two different extraction procedures were carried out. After fatty acid characterization, degree of acidity, peroxide index and a basic sensory analysis were performed. Antioxidant in vitro activity and total phenolic compounds (TPC) were also determined. This new ingredient showed a better sensory profile than raw ham fat, a lower degree of acidity, a higher content of MUFAs, and also showed a higher antioxidant capacity and an increase in phenolic compounds compared to the raw material. This bioactive essence could be used as a food, a cosmetic or a nutraceutical ingredient to prevent certain diseases related to oxidative stress and could also contribute to the maintenance of the circular economy.

Antibiofilm activity and bioactive phenolic compounds of ethanol extract from the Hericium erinaceus basidiome.

Biofilm formation has become a serious health and environmental problem. Mushrooms are now considered a valuable source of bioactive compounds with antimicrobial properties. The lion's mane mushroom (Hericium erinaceus [HE]) has been used as an antimicrobial for ulcers and gastritis in East Asian countries. However, studies on the antibiofilm activities of HE basidiome against biofilm-forming pathogenic bacteria and their bioactive compound profiles are still limited. The purpose of this study was to determine the antibiofilm activity of HE and to identify its phenolic compound profile. The HE inhibitory activities against bacterial growth and biofilm formation were performed against Pseudomonas aeruginosa, Salmonella Typhimurium, Proteus mirabilis, and Staphylococcus aureus. Remarkably, P. mirabilis was the most susceptible bacteria to HE. The total phenolic content (TPC) of HE was 1652 ± 1.06 µg/ml, with protocatechuic acid and p-coumaric acid being the most abundant phenolic compounds as determined by high-performance liquid chromatography-mass spectrophotometry (HPLC-MS). This research highlights the possibility of HE as an antibiofilm agent that can be developed as a nutraceutical and natural food preservative.

Assessment of the influence of the mineral complex of rutin on the degree of expression of anti-diabetic activity

Assessment of the influence of the mineral complex of rutin on the degree of expression of anti-diabetic activity