Activity Of Hydroalcoholic Extract Research Articles

Evaluation of Anti-ulcer Activity of Hydro Alcoholic Extracts of Abutilon indicum, Helianthus annuus and Combination of Both against Ethanol and Pyloric Ligation Induced Gastric Ulcer in Albino Wistar Rats

1 Department of Pharmaceutics, Jawaharlal Nehru Technological University Anantapur, Oil Technological Research Institute, Ananthapuramu-515 001, Andhra Pradesh, India. 2 Department of Pharmacology, Jawaharlal Nehru Technological University Anantapur, Oil Technological Research Institute, Ananthapuramu-515 001, Andhra Pradesh, India. 3 Department of Chemistry, Jawaharlal Nehru Technological University Anantapur, Oil Technological Research Institute, Ananthapuramu-515 001, Andhra Pradesh, India. 4 Department of Chemistry, Jawaharlal Nehru Technological University Anantapur, Ananthapuramu515 002, Andhra Pradesh, India.

Invitro Anti-mycotic Activity of Hydro Alcoholic Extracts of Some Indian Medicinal Plants against Fluconazole Resistant Candida albicans.

Candidiasis is one of the most common opportunistic infections caused by Candida albicans. Fluconazole is the drug of choice for prevention and management of this condition. However, the emergence of fluconazole resistant candidal strains has become a major concern. Many herbs like fenugreek, cinnamon, papaya, oregano, garlic are rich in phytochemical constituents known to express antimycotic activity. With the available information, the present research study was carried out to assess the invitro anti-mycotic activity of hydro alcoholic extracts of Trigonella foenum-graecum seeds, Cinnamomum verum bark and Carica papaya leaves and seeds against fluconazole resistant Candida albicans. Hydro alcoholic extracts of Trigonella foenum-graecum (seeds), Cinnamomum verum (bark), Carica papaya CO.2 strain (male and female leaves) and Carica papaya CO.2 strain (seeds) were prepared by maceration. The anti-mycotic activity of the prepared extracts against Candida albicans was assessed by agar well diffusion method. Three independent experiments were performed in triplicates and the mean and standard deviation were calculated. Minimum inhibitory concentration was determined. The results of the present study revealed that all the extracts exhibited anti-mycotic activity in a dose dependent manner and minimum inhibitory concentration of all the extracts was found to be 15.62 μg/ml. The results of the present study shed light on the fact that plant extracts could be used not only as an alternate drug for management of fluconazole resistant candidiasis but also explored further for oral cancer prevention as a therapeutic adjunct.

Extraction and Evaluation of Anti-helminthic Activity of Hibiscus Cannabis. L

According to the literature review, it was found that various activities was done and reported on Hibiscus cannabis L., But anthelminthic activity of the leaves of the plant Hibiscus Cannabis L were not reported. So, my present work is aimed to carry out the Extraction & Isolation, Phytochemical screening, and estimation of anti-helminthic activity, anti-oxidant activity of hydro alcoholic extract, ethanolic extract, and water extract of Hibiscus Cannabis L.

Evaluation of anti asthmatic activity of hydro alcoholic extract of Citrullus colocynths and Cucumis trigonus fruits

The present study aimed to evaluate the anti-asthmatic activity of the hydro-alcoholic extract of Citrullus colocynths and Cucumis trigonus (Cucurbitaceae) fruits on Ovalbumin induced Asthma in rats. Extracts were prepared with 70% v/v ethanol using cold maceration followed by Soxhlation. Hydro alcoholic extract (200 and 400 mg/kg) of both fruits were evaluated for anti asthmatic activity on ovalbumin induced Asthma in rats with the help of parameters like, absolute eosinophil count in BALF, total leukocyte count in the BALF, absolute eosinophil count in the Blood, IgE antibodies in serum and histopathological findings of lungs. Both plants were showed significant anti asthmatic activity (p < 0.001) with dose of 200 & 400 mg/kg when compared with disease group. Obtained results were almost similar to that of normal group. With the same dose, C. colocynths was shown better anti asthmatic activity than C. trigonus. Hence, further detailed studies are required to evaluate the efficacy of these plants on anti-asthmatic activity.

Hepatoprotective potential of antioxidant potent fraction from Urtica dioica Linn. (whole plant) in CCl4 challenged rats

The aim of the present study was to isolate hepatoprotective component from Urtica dioica Linn. (whole plant) against CCl4-induced hepatotoxicity in-vitro (HepG2 cells) and in-vivo (rats) model. Antioxidant activity of hydro alcoholic extract and its fractions petroleum ether fraction (PEF), ethyl acetate fraction (EAF), n-butanol fraction (NBF) and aqueous fraction (AF) were determined by DPPH and NO radicals scavenging assay. Fractions were subjected to in-vitro HepG2 cell line study. Further, the most potent fraction (EAF) was subjected to in-vivo hepatoprotective potential against CCl4 challenged rats. The in-vivo hepatoprotective active fraction was chromatographed on silica column to isolate the bioactive constituent(s). Structure elucidation was done by using various spectrophotometric techniques like UV, IR, 1H NMR, 13C NMR and MS spectroscopy. Ethyl acetate fraction (EAF) of hydro-alcoholic extract of U. dioica possessed the potent antioxidant activity viz. DPPH (IC50 78.99±0.17μg/ml) and NO (IC50101.39±0.30μg/ml). The in-vitro HepG2 cell line study showed that the EAF prevented the cell damage. The EAF significantly attenuated the increased liver enzymes activities in serum and oxidative parameters in tissue of CCl4-induced rats, suggesting hepatoprotective and anti-oxidant action respectively. Column chromatography of most potent antioxidant fraction (EAF) lead to the isolation of 4-hydroxy-3-methoxy cinnamic acid (ferulic acid) which is responsible for its hepatoprotective potential. Hence, the present study suggests that EAF of hydro-alcoholic extract has significant antioxidant and hepatoprotective potential on CCl4 induced hepatotoxicity in-vitro and in-vivo.

Hepatoprotective activity ofchenopodium albumLinn. in carbon tetrachloride induced hepatotoxicity rats

Chenopodium album Linn. is a commonly used herbal drug against many diseases. The hepatoprotective activity of hydroalcoholic extract of dried powder of leaves plant of Chenopodium album Linn. was investigated in hepatotoxicity rat model. Hepatotoxicity was induced in Wistar rats by intraperitoneal injection of carbon tetrachloride. Different doses were tested to decide the dose related hepatoprotective efficacy of Chenopodium album Linn. (200 mg/kg, 400 mg/kg, 600 mg/kg body weight/day po for two weeks). The Hepatoprotective effect of these extracts was evaluated by liver function biochemical parameters (i. e. total bilirubin, total cholesterol, total protein, serum glutamateoxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and alkaline phosphatase and In Vivo antioxidant enzyme superoxide dismutase (SOD), reduced glutathione (GSH), lipid peroxidases (LPO) and catalase activities) and histopathological examination of liver. In extract-treated animals, the toxicity effect of carbon tetrachloride was controlled significantly by restoration of the levels of serum biochemical parameters as compared to the normal and standard drug silymarin treated groups. Histology of liver sections of the animals treated with the extracts showed the hepatic cell regeneration. Therefore, the results of present study support the hepatoprotective effect of Chenopodium album Linn.

The Hypoglycemic Effects of an Ethanol Extract of Peganum harmala in Streptozotocin-Induced Diabetic Rats

Peganum harmala is a plant that is traditionally used as an analgesic, anti-inflammatory, antibacterial, antioxidant, anti-helmintic, and antimutagenic agent. Moreover, it is used to treat a variety of human ailments, including depression. This study was conducted to investigate the antidiabetic activity of hydro alcoholic extract of this herb. A hydro alcoholic extract from seeds of this herb was prepared and administered at three doses of 30, 60 and 120 mg/kg to three groups of streptozotocin-induced diabetic rats. Two additional groups were used as negative (normal saline plus solvent) and positive control (metformin). Blood glucose levels in animals from all groups were measured at 2, 4, and 6 hours after intraperitoneal injection of the extract to rats. Blood glucose levels decreased in the diabetic rats in comparison with normal rats (P < 0.05). Our finding showed that P. harmala seed extract has good antidiabetic activity in streptozotocin-induced diabetic rats. Further studies need to isolate active compounds and to investigate their activity.

Cytotoxic and apoptogenic properties of Nigella sativa and thymoquinone, its constituent, in human renal cell carcinoma are comparable with cisplatin

Renal cell carcinoma (RCC) is the most common renal malignancy in adults and exhibits highly intrinsic and acquired resistance to standard therapeutic strategies. We sought to determine the anti-cancer activity of hydroalcoholic extract of Nigella sativa seeds (NSE) and thymoquinone (TQ). Human renal cell carcinoma (ACHN) and fibroblast L929 cell lines were treated with NSE and TQ, and cytotoxicity was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) assay. Cell death pattern was determined by annexin V and propidium iodine (PI)-staining methods. Exposure to NSE, TQ and cisplatin significantly inhibited the growth of ACHN cells and showed significant increase of early apoptotic cells. Normal cells were more resistant to NSE and TQ-induced effects. The present study demonstrates that N. sativa and TQ exert anti-proliferative and pro-apoptotic effects on ACHN cells in a concentration and time-dependent manner, which suggests their potential to be used as a new therapeutic strategy for renal cancers.

Comparative Evaluation of the Antifungal Activity of Borage (Borago officinalis) and Camellia sinesis (Green tea) leaves against various Aspergillus and Candida strains

The current study aimed to evaluate and compare the antifungal activity of hydroalcoholic extracts of Borage officinalis and Camellia sinesis (Green tea) against different Candida and Aspergillus strains. Antifungal effects of extracts were determined by disc diffusion and broth macrodilution methods. The Borago officinalis extract showed the highest antifungal activity against A. niger and the lowest effect against C. kreusei, C. dubliniensis, A. fumigatus and A. flavus. The Green tea extract revealed high antifungal activity against C. guilliermondii and C. dubliniensis and less effect against C. parapsilosis and A. fumigatus. The MIC values for Borago officinalis and Green tea extracts were ranged from 1.56 to 25 mg/ml and 3.125 to 25 mg/ml, respectively. This study suggests that Borago officinalis and Green tea are valuable plant sources of medicinally useful compounds that this may help to avoid the side effects of antifungals. The combined use of Green tea or Borago officinalis extracts and antifungals could be also useful in fighting emerging drug-resistant problem among fungal pathogens.

Antidiabetic activity of Annona squamosa Linn. in alloxan - induced diabetic rats

Background: Diabetes mellitus is characterised by rise in blood sugar levels resulting from insulin dysfunction or insulin insufficiency. Aim: The aim of the present investigation is to evaluate antidiabetic activity of hydroalcoholic extract of Annona squamosa Linn (A. squamosa Linn) in alloxan‑induced diabetic rat model. Materials and Methods: Diabetes is induced by a single‑dose intraperitoneal injection (i.p.) of alloxan (120 mg/kg) to albino rats. Results and Discussion: Treatment with A. squamosa Linn. extract at a dose of 350 mg/kg and 700 mg/kg and glibenclamide at a dose of 5mg/kg for 28 days, after induction of diabetes by alloxan, caused significant reduction in blood serum glucose and serum lipid profiles like total cholesterol and triglycerides but significant increase in body weight and serum high density lipoproteins (HDL) level in diabetic rats compared to untreated group. Histological study of the pancreas of diabetic rat treated with A. squamosa extract also showed partial regeneration of beta cells. The antidiabetic activity of this extract is found comparable to glibenclamide. Thus, leaves of A. squamosa Linn. can be used as potential antidiabetic drug. Key words: Annona squamosa, antidiabetic, glibenclamide

Nephroprotector activity of hydro alcoholic extract of Tinospora cordifolia roots on cisplatin induced nephrotoxicity in rats

ObjectiveTo investigate the protective role of the Tinospora cordifolia root extract against cisplatin induced nephrotoxicity in albino rats. MethodsIn the present study, the ethanol extract of roots of T. cordifolia (200 mg & 400 mg/kg bd.wt) was examined for its protective effect against cisplatin induced renal injury in rats. Thirty healthy male and female albino rats (150–200 g weight) were chosen and divided into five groups. Vehicle, cisplatin and ethanolic extract were given according to the experimental design. After 8 days of treatment, urinary functional parameters were analysed. ResultsHistopathological characters were examined after scarifying and dissecting the rats. Cisplatin (6 mg/kg) alone significantly elevated serum markers level, increased urinary protein excretion, reduced urine to serum creatinine ratio and creatinine clearance. In curative regimen, the extract significantly reduced the elevated serum creatinine and urea levels. Renal antioxidant defense systems, such as superoxide dismutase, catalase, glutathione peroxides activities and reduced glutathione level, depleted by cisplatin therapy were restored to normal by treatment with the extract. ConclusionThe present investigation suggests that the hydro alcoholic extract of roots of T. cordifolia has protective effect against cisplatin induced nephrotoxicity.

EUPHORBIA NERRIFOLIA LINN: HERBAL TOOL FOR BIRTH CONTROL

In the present study, antifertility activity of hydr oalcoholic extract of Euphorbia neriifolia Linn was evaluated. The ethanolic extract of Euphorbia neriifolia showed significant antifertility activity Pre treatment with ethanolic extract showed significant inhibition of number of implant site at a dose of 400 mg/kg. There was no change in ovulation, hence the antifertility activity observed in the present study with Euphorbia neriifolia can be attributed largely to its anti implantation activity.

Antifertility Activity of Hydroalcoholic Extract of Ocimum Basilicum Linn. Leaves on Female Wistar Rats

Objective To determine the effect of hydroalcoholic extract of Ocimum basilicum Linn. leaves on ovulation, implantation and maintenance of pregnancy in adult female cyclic Wistar rats. Methods Ocimum basilicum leaves extract (364 mg/kg and 624 mg/kg) was screened for its antiovulatory, antiimplantation and abortifacient activities. Vaginal smear of each rat was monitored daily during the 15-day treatment of the first experiment. Estimation of cholesterol in ovarian tissue and its histology was carried out to confirm its antiovulatory activity. In the second and third experiments, animals received treatment from 1st to 7th and 8th to 16th day of pregnancy, respectively. The number of implantations and litter size were determined through laparotomy on 10th and 20th day of pregnancy, respectively in two tests. The treated groups were compared with pain control. Results A significant increase in duration of estrus cycle and diestrus phase was observed (P Conclusions Hydroalcoholic extract of Ocimum basilicum interferes normal ovulation by disrupting the estrus cycle and prolonging diestrus phase thus, has a potential of being developed into a female contraceptive.

In Vitro Antioxidant and Antihemolytic Activities of Hydroalcoholic Extracts of Allium scabriscapum Boiss. & Ky. Aerial Parts and Bulbs

This study is designed to evaluate the antioxidant and antihaemolytic activities of the hydroalcoholic extracts of Allium scabriscapum aerial parts and bulbs by employing eight in vitro assay systems. In 1,1-diphenyl-2-picryl hydrazyl radical scavenging assay, both the extracts show moderate scavenging activity. The reducing power ability of extracts increased with increasing in the samples concentrations. IC50 for metal chelating activity of aerial parts and bulbs extracts were 894.6 ± 31.29 and 746.2 ± 26.11 μg mL−1, respectively. The aerial part extracts show better nitric oxide and hydrogen peroxide scavenging activities than the bulb. Extracts exhibited good antioxidant activity in linoleic acid emulsion system and were comparable to vitamin C (p > 0.05). Aerial parts extract showed better antihemolytic activity against cumene hydroperoxide and hydrogen peroxide-induced hemolysis. Among the extracts A. scabriscapum, aerial parts had higher phenolic and flavonoid contents.

In vitro antioxidant activity of hydro alcoholic extract from the fruit pulp of Cassia fistula Linn.

The present study is aimed to investigate antioxidant activity of the extracts of Cassia fistula Linn. (Leguminosae) fruit pulp. Cassia fistula Linn., a Indian Laburnum, is widely cultivated in various countries and different continents including Asia, Mauritius, South Africa, Mexico, China, West Indies, East Africa and Brazil as an ornamental tree for its beautiful bunches of yellow flowers and also used in traditional medicine for several indications. The primary phytochemical study and in vitro antioxidant study was performed on hydro alcoholic extract of fruit pulp. Phytochemical screening of the plant has shown the presence of phenolic compounds, fatty acids, flavonoids, tannins and glycosides. Phenolic content was measured using Folin-Ciocalteu reagent and was calculated as gallic acid equivalents. Antiradical activity of hydro alcoholic extract was measured by DPPH (2,2-diphenyl-1- picrylhydrazyl) assay and was compared to ascorbic acid. Ferric reducing power of the extract was also evaluated by Oyaizu method. In the present study, three methods were used for evaluation of antioxidant activity. First two methods were for direct measurement of radical scavenging activity and third method to evaluate the reducing power. Results indicate that hydro alcoholic fruit pulp extracts have marked amount of total phenols which could be responsible for the antioxidant activity. These in vitro assays indicate that this plant extract is a significant source of natural antioxidant, Cassia fistula fruit pulp extract shows lower activity in DPPH and total phenol content as compared with standard which might be helpful in preventing the progress of various oxidative stresses.

EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF AGLAIA ELEAGNOIDEA (A. JUSS) BENTH STEM EXTRACT AGAINST CARBON TETRACHLORIDE INDUCED HEPATOTOXICITY IN ALBINO RATS

Objective: The present study was intended to evaluate the hepatoprotective activity of hydro alcoholic extract of stem of Aglaia eleagnoidea (A. Juss) Benth. It is a medicinal plant found in the dense and moist forests in Western and Eastern Ghats, and also in many drier parts of Indian states like Andhra Pradesh, Karnataka, Kerala, Bihar, Andaman and Nicobar Islands. Method: The hydro alcoholic extract and its hexane, ethyl acetate and methanol fractions of Aglaia eleagnoidea (A. Juss) Benth., at the doses of 150,300 & 600 mg/kg and two isolated phytoconstituents (50mg/kg) was administered orally by suspending in Sodium CMC to the Wister albino rats with carbon tetra chloride(1ml/kg) induced hepatotoxicity. Silymarin (50mg/kg) was given as reference standard drug. This dose dependant study was followed by histopathological studies of ccl4 and plant extract treated liver tissue. Result: Studies of blood samples of carbon tetra chloride treated rats showed significant increase in the levels of serum enzyme activities, reflecting the liver injury caused by paracetamol. It was evident from the results that after treatment with the plant extract, there was a significant reduction in the levels of serum biochemical parameters serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALKP) and total bilirubin (TBL) induced by carbon tetrchloride hepatotoxicity. The histopathological observations also showed that plant extract treated liver sections against carbon tetrchloride induced hepatotoxisity revealed the well preserved cellular architexture of the liver tissue. Conclusion: The present study indicates the potential hepatoprotective activity of Aglaia eleagnoidea stem and its bioactive phytoconstituents predominantly stigmasterol, ?-sitosterol, Daucosterol and unidentified polyphenols. These compounds probably by suppressing the activity of reactive oxygen species which cause cellular damage, there by offering protection to the targeted tissue against the hepatotoxicity induced by carbon tetra chloride in albino rats.

Antinociceptive and anti-inflammatory potential of extract and isolated compounds from the leaves of Salvia officinalis in mice

Ethnopharmacological relevanceSalvia officinalis L. has been used as a traditional herbal medicine for gastric disturbances and inflammatory processes. This study investigated the toxicological, antinociceptive and anti-inflammatory effects of the hydroalcoholic extract (HE) from leaves of Salvia officinalis and its isolated compounds in mice. Materials and methodsMice were treated with HE before the induction of nociceptive response by chemical agents (acetic-acid, formalin, glutamate, capsaicin and cinnamaldehyde). Total leukocytes and plasma extravasation induced by acetic acid and paw oedema induced by glutamate, capsaicin and cinnamaldehyde were also measured. The antinociceptive effect of carnosol and ursolic acid/oleanolic acid were evaluated on formalin and cinnamaldehyde models. ResultsIn the acute toxicity test the value of estimated LD50 for HE was 44.7579g/kg. Oral administration of HE (10, 30 and 100mg/kg) inhibited the number of writhings, total leukocytes and plasma extravasation induced by acetic acid. In the formalin test, HE reduced both neurogenic and inflammatory phases, effect that was affected by naloxone. The glutamate-, capsaicin- and cinnamaldehyde-induced nociception and paw oedema were reduced by HE at doses that did not affect the locomotor activity of mice in the open field test. Carnosol (10mg/kg) and ursolic acid/oleanolic acid (30mg/kg) inhibited the inflammatory phase of formalin and the nociception and mechanical allodynia induced by cinnamaldehyde. ConclusionsThese results demonstrate that HE presents significant anti-inflammatory and also antinociceptive effects on chemical behavioral models of nociception that involves an opioid mechanism. In addition, carnosol and ursolic acid/oleanolic acid contained in this plant appears to contribute for the antinociceptive property of the extract, possibly through a modulatory influence on TRPA1-receptors. However, further studies regarding the precise site and the mechanism of action of HE and carnosol and ursolic acid/oleanolic acid merited exploring further.

Antioxidant Activity of Hydroalcoholic Extract of Labisa Pumila var. Alata, an Indigenous Plant of Malaysia

This study was carried out to investigate the antioxidant potential of crude extracts of Labisa pumila var. Alata, a small herbaceous under shrub widely available in Malaysia. In this study, antioxidant activity was evaluated by diphenyl picryl hydrazine (DPPH) free radical assay and FRAP assay methods. In the DPPH free radical assay, different concentrations of the extract (5, 10, 25, 50 and 75 μg/ml in ethanol) were prepared and mixed with DPPH ethanol solution. After incubation, a photometric assay was performed and values were plotted. The IC50 values were calculated by a linear regression of plots and compared with IC50 of ascorbic acid as the standard antioxidant. In the FRAP assay, different concentrations of extract (20, 40, 60, 80 and 100 μg/ml) were prepared and the reducing power was estimated by a photometric assay and values were plotted. The EC50 values were calculated by a linear regression of plots and compared with the EC50 of ascorbic acid as a standard reducing agent. The study revealed that the crude extract possesses antioxidant activity.

Reversible Antifertility Activity of Hydroalcoholic Extract of Ficus Racemosa L. in Male Mice

Objective To evaluate male antifertility activity of hydroalcoholic extract of Ficus racemosa bark. Methods Swiss male mice were orally administered hydroalcoholic extract of Ficus racemosa bark (50 mg/kg for 30 d and 100 mg/kg body weight for next 30 d), and the effect of the treatment on body weight, reproductive organs weight, sperm, biochemical profile (sialic acid in epididymis and fructose in seminal vesicle), fertility and vaginal contraceptive efficacy was investigated. Recovery studies were also performed. Results Extract reduced fertility to 70% within 60 d. Suppression of cauda epididymis sperm count, motility, viability and abnormal morphology was observed. Marked reduction was noted in the weight of reproductive organs and the level of sialic acid in epididymis and fructose in seminal vesicle. Vaginal application of bark extract exhibited 80% vaginal contraceptive efficacy. After cessation of plant extract treatment, the altered parameters recovered after 60 d. Conclusion Clinical assessment of male antifertility agents should include acceptability, safety and efficacy during and after the treatment. The above results revealed the potential, reversible male antifertility effect of hydroalcoholic extract F. racemosa bark.

Evaluation of in vitro Antioxidant Activity of hydroalcoholic seed extratcs of Cassia fistula linn.

Antioxidant activity has been assessed by In vitro method for phytochemical fraction of plant, viz hydro alcohol extract of Cassia fistula seeds. The present study was aimed to investigate the antioxidant activity of extracts of dried seed powder of Cassia fistula Linn. (Family: Leguminosae). Cassia fistula Linn. has been reported to possess hypoglycaemic, anticancer, abortifacient, anticolic, antifertility, estrogenic, laxative, antimicrobial, antipyretic, anti-inflammatory, smooth muscle stimulant, antiarthritic, antitussive, purgative, analgesic, antiviral, hepatoprotective, and anti implantation activity. The primary phytochemical study and in vitro antioxidant study was performed on hydro alcoholic extract of shade dried seeds. Modern phytochemical screening of the plant has shown the presence of phenolic compounds, fatty acids, flavonoids, tannins and glycosides. The extracts were evaluated for their phenolic content & antioxidant activity. Phenolic content was measured by using Folin-Ciocalteu reagent & was calculated as Gallic acid equivalents. Antiradical activity of hydro alcoholic extract was measured by DPPH (2,2-diphenyl-1-picryl hydrazyl) assay & was compared to ascorbic acid (vitamin C), and Ferric reducing power of the extract was also evaluated by Oyaizu method. In the present study three methods were used for evolution of antioxidant activity. The first two methods were for direct measurement of radical scavenging activity & remaining one method evaluated the reducing power. The present study revealed that the hydro alcoholic extracts of seeds have significant radical scavenging activity. In this study, Cassia fistula was identified as potentially novel source of free radical scavenging compound. The results clearly indicate that Cassia fistula is effective against free radical mediated diseases and also helpful to draw special attention for further studies.