Antioxidant Activity of Hydroalcoholic Extract of Labisa Pumila var. Alata, an Indigenous Plant of Malaysia

Abstract

This study was carried out to investigate the antioxidant potential of crude extracts of Labisa pumila var. Alata, a small herbaceous under shrub widely available in Malaysia. In this study, antioxidant activity was evaluated by diphenyl picryl hydrazine (DPPH) free radical assay and FRAP assay methods. In the DPPH free radical assay, different concentrations of the extract (5, 10, 25, 50 and 75 μg/ml in ethanol) were prepared and mixed with DPPH ethanol solution. After incubation, a photometric assay was performed and values were plotted. The IC50 values were calculated by a linear regression of plots and compared with IC50 of ascorbic acid as the standard antioxidant. In the FRAP assay, different concentrations of extract (20, 40, 60, 80 and 100 μg/ml) were prepared and the reducing power was estimated by a photometric assay and values were plotted. The EC50 values were calculated by a linear regression of plots and compared with the EC50 of ascorbic acid as a standard reducing agent. The study revealed that the crude extract possesses antioxidant activity.