Activity Of Copper Oxide Nanoparticles Research Articles

Characterization And Antimicrobial Activity of Copper Oxide Nanoparticles Synthesized by Crocus Sativus Extract

This study aimed to evaluate the antimicrobial activity of green synthesized copper-oxide nanoparticles (CuO-NPs) using Crocus sativus. The microorganisms were supplied from Baghdad Teaching Hospital. Using C. sativus, CuO nanoparticles were synthesized in a green manner, and their properties were assessed using UV-visible spectroscopy at the peak of 260 nm. Atomic force microscopy (AFM) was used to measure the particles' size and form, and x-ray technology was used to more precisely quantify their dimensions. diffraction (XRD). The biomolecule and functional group were determined by Fourier transform infrared spectroscopy (FTIR). Different concentrations of nanoparticles (50, 75, and 100 ?g/ml) were subjected to the well diffusion method for antibacterial activity testing. The findings indicated that the CuO-NPs had a spherical form and a 0.154 nm size. The average diameters of the AFM pictures were found to be 11.89 nm, 2.62 nm, and 24.55 nm. The following growth zone inhibition values for the various bacteria species used were revealed by the nanoparticles' antimicrobial activity results: Staphylococcus aureus 18 mm, Staphylococcus epidermidis 18 mm, Klebsiella ssp 15 mm, Candida albican 18 mm, and Escherichia coli 17mm.

Antifungal activity of copper oxide nanoparticles derived from Zizyphus spina leaf extract against Fusarium root rot disease in tomato plants

Incorporating green chemistry concepts into nanotechnology is an important focus area in nanoscience. The demand for green metal oxide nanoparticle production has grown in recent years. The beneficial effects of using nanoparticles in agriculture have already been established. Here, we highlight some potential antifungal properties of Zizyphus spina leaf extract-derived copper oxide nanoparticles (CuO-Zs-NPs), produced with a spherical shape and defined a 13–30 nm particle size. Three different dosages of CuO-Zs-NPs were utilized and showed promising antifungal efficacy in vitro and in vivo against the selected fungal strain of F. solani causes tomato root rot disease, which was molecularly identified with accession number (OP824846). In vivo results indicated that, for all CuO-Zs-NPs concentrations, a significant reduction in Fusarium root rot disease occurred between 72.0 to 88.6% compared to 80.5% disease severity in the infected control. Although treatments with either the chemical fungicide (Kocide 2000) showed a better disease reduction and incidence with (18.33% and 6.67%) values, respectively, than CuO-Zs-NPs at conc. 50 mg/l, however CuO-Zs-NPs at 250 mg/l conc. showed the highest disease reduction (9.17 ± 2.89%) and lowest disease incidence (4.17 ± 3.80%). On the other hand, CuO-Zs-NPs at varied values elevated the beneficial effects of tomato seedling vigor at the initial stages and plant growth development compared to either treatment with the commercial fungicide or Trichoderma Biocide. Additionally, CuO-Zs-NPs treatments introduced beneficial results for tomato seedling development, with a significant increase in chlorophyll pigments and enzymatic activity for CuO-Zs-NPs treatments. Additionally, treatment with low concentrations of CuO-Zs-NPs led to a rise in the number of mature pollen grains compared to the immature ones. however the data showed that CuO-Zs-NPs have a unique antifungal mechanism against F. solani, they subsequently imply that CuO-Zs-NPs might be a useful environmentally friendly controlling agent for the Fusarium root rot disease that affects tomato plants.Graphical

Biosynthesis and antimicrobial activity of copper oxide nanoparticles

Biosynthesis and antimicrobial activity of copper oxide nanoparticles

Green Synthesis of Copper Oxide Nanoparticles from the Leaves of Aegle marmelos and Their Antimicrobial Activity and Photocatalytic Activities.

The leaves of the Aegle marmelos plant were used for the green synthesis of copper oxide nanoparticles and further characterized by different techniques, including (Ultra Violet-Visible) UV-Vis, Scanning electron microscopy (SEM), Energy dispersive X-ray (EDX), Transmission electron microscopy (TEM) and X-ray diffraction (XRD). The UV-Vis showed a peak at 330 nm, which may be due to the Surface Plasmon Resonance phenomenon. XRD analysis showed the crystalline nature of copper oxide nanoparticles (CuO NPs). In contrast, SEM showed that nanoparticles were not aggregated or clumped, EDX showed the presence of elemental copper., and further, the TEM analysis revealed the average particle size of copper oxide nanoparticles to be 32 nm. The Minimum Inhibitory Concentration (MIC) for Escherichia coli (E. coli) and Staphylococcusaureus (S. aureus) was found to be 400 µg/mL, whereas for Candida albicans (C. albicans) and Candida dubliniensis (C. dubliniensis) it was 800 µg/mL. The zone of inhibition in the well diffusion assay showed the antimicrobial activity of copper oxide nanoparticles, and it also showed that as the concentration of copper oxide nanoparticles increased, the zone of inhibition also increased. Further, the electron microscopic view of the interaction between copper oxide nanoparticles and C. albicans cells showed that CuO NPs were internalized and attached to the cell membrane, which caused changes in the cellular structure and caused deformities which eventually led to cell death. The prepared CuO NPs showed significant photocatalytic degradation of organic dyes in the presence of sunlight.

Enriched biological activity of copper oxide nanoparticles derived from Aloe vera extract

Enriched biological activity of copper oxide nanoparticles derived from Aloe vera extract

The Antibacterial Activity of the Biosynthesized Copper Oxide Nanoparticles by Lantana camara Flowers Extract Against Some Bacterial Isolated from Burns

The study included 70 Surface swabs collected from patients in different ages of both genders suffering from burns infections. All the specimens were diagnosed by subculturing in differential and selective media, microscopically and by following the biochemical tests to confirm identifications. Antibiotic susceptibility test was done to all the bacterial isolates.The identification of the clinical isolates showed that the 16(22.9%) were belonged to Staphylococcus aureus and 6(8.6%) of Staphylococcus epidermidis , whereas 23(32.8%),20(28.6%) isolates were for Pseudomonas aeruginosa ,Acinetobacter baumannii respectively. Copper oxide nanoparticles were biosynthesized by Lantana camara flower extracts which acted as a reducing agent for copper nitrate salts, characterization of the metal oxide was done by using Ultra violate visible light Spectroscopy, Fourier transforms Infrared spectroscopy, X-ray Diffraction, Atomic Force Microscopy and Field Emission Scanning Electron Microscopy. The biological activity of Copper Oxide Nanoparticles was screened toward three clinical isolates that showed multiple drug resistance isolated from burns by following agar wells diffusion method which proved the ability of copper oxide nanoparticles to inhibit growth of the clinical bacterial isolates. The minimum inhibitory concentrations were determined toward the tested bacteria by using the microdilution method.

Effect of H2O2 @CuONPs in the UV Light-Induced Removal of Organic Pollutant Congo Red Dye: Investigation into Mechanism with Additional Biomedical Study.

Hazardous dyes in industrial wastewater are an internationally recognized issue for community health. Nanoparticles synthesized through green protocols are a fascinating research field with numerous applications. The current study mainly aimed to investigate the degradation of Congo red (CR) dye under UV light in the presence of H2O2 and the photocatalytic activity of copper oxide nanoparticles (CuONPs). For CuONP formation, Citrus maxima extract contains a high number of phytochemical constituents. The size of CuONPs ranges between 25 and 90 nm. The photocatalytic activity of CuONPs with the addition of H2O2 was observed and analyzed under UV light to eliminate CR dye. The UV light caused the decomposition of H2O2, which produced ·OH radicals. The results revealed a significant increment in dye degradation during the presence of H2O2. The effect of concentration on the degradation of the CR dye was also studied. The degradation pathway of organic pollutants was reputable from the hydroxy radical medicated degradation of CR. Advanced Oxidation Treatment depends on the in situ production of reactive ·OH species and is presented as the most effective procedure for decontamination. The biological activity of CuONPs was evaluated against Escherichia coli Bacillus subtillis, Staphylococcus aureus, Shigella flexenari, Acinetobacter Klebsiella pneumonia, Salmonella typhi and Micrococcus luteus. The newly synthesised nanomaterials showed strong inhibition activity against Escherichia coli (45%), Bacillus subtilis (42%) and Acinetobacter species (25%). The activity of CuONPs was also investigated against different fungus species such as: Aspergillus flavus, A. niger, Candida glabrata, T. longifusus, M. Canis, C. glabrata and showed a good inhibition zone against Candida glabrata 75%, Aspergillus flavus 68%, T. longifusus 60%. The materials showed good activity against C. glaberata, A. flavus and T. longifusus. Furthermore, CuONPs were tested for antioxidant properties using 2, 2 diphenyl-1-picrylhydrazyl) (DPPH).

Antifungal Activity of Copper Oxide Nanoparticles against Root Rot Disease in Cucumber.

Metal oxide nanoparticles have recently garnered interest as potentially valuable substances for the management of plant diseases. Copper oxide nanoparticles (Cu2ONPs) were chemically fabricated to control root rot disease in cucumbers. A scanning electron microscope (SEM), X-ray diffraction (XRD) and photoluminescence (PL) were employed to characterize the produced nanoparticles. Moreover, the direct antifungal activity of Cu2ONPs against Fusarium solani under laboratory, greenhouse, and field conditions were also evaluated. In addition, the induction of host-plant resistance by Cu2ONPs was confirmed by the results of enzyme activities (catalase, peroxidase, and polyphenoloxidase) and gene expression (PR-1 and LOX-1). Finally, the effect of Cu2ONPs on the growth and productivity characteristics of the treated cucumber plants was investigated. The average particle size from all the peaks was found to be around 25.54 and 25.83 nm for 0.30 and 0.35 Cu2O, respectively. Under laboratory conditions, the study found that Cu2ONPs had a greater inhibitory effect on the growth of Fusarium solani than the untreated control. Cu2ONP treatment considerably reduced the disease incidence of the root rot pathogen in cucumber plants in both greenhouse and field environments. Defense enzyme activity and defense genes (PR1 and LOX1) transcription levels were higher in cucumber plants treated with Cu2ONPs and fungicide than in the untreated control. SEM analysis revealed irregularities, changes, twisting, and plasmolysis in the mycelia, as well as spore shrinking and collapsing in F. solani treated with Cu2ONPs, compared to the untreated control. The anatomical analysis revealed that cucumber plants treated with Cu2ONPs had thicker cell walls, root cortex, and mesophyll tissue (MT) than untreated plants. Cucumber growth and yield characteristics were greatly improved after treatment with Cu2ONPs and fungicide. To the best of our knowledge, employing Cu2ONPs to treat cucumber rot root disease is a novel strategy that has not yet been reported.

Enhanced photocatalytic and Antibacterial Activity of Copper oxide Nanoparticles Synthesized by Facile Combustion methods from Mussaendafrondosa Plant Extract

Microwave heating (MHM) and mutated sol-gel (SGM) mechanisms were used to effectively create CuO samples with two dissimilar morphologies, such as nanoparticles (CuO-NPs) and nanorods (CuO-NRs), using Mussaendafrondosalinn plant extract as the bio-reducing operator. X-ray powder diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray (EDX) investigations were used to analyze the sample's structure, pureness, and morphological characteristics. UV-Visible diffuse reflectance (DRS) and photoluminescence (PL) spectroscopy methodologies were used to analyze optical properties and calculate bandgap energy. The bandgap of the samples was measured using the Kubelka-Munk mechanism, and it was found to be 2.74eV and 2.33eV for CuO-NPs and CuO-NRs, correspondingly. CuO-NPs and CuO-NRs were investigated for antibacterial activity versus each Gram-positive and Gram-negative microorganisms using a modified disc diffusion method. When correlated to the sample CuO nanorods, the antibacterial study confirms that the sample CuO nanoparticles are high-grade antibacterial agents. Using solar lighting, the photocatalytic activity of CuO nano-reactants (CuO-NPs and CuO-NRs) for the degradation of methylene blue (MB) dye was investigated, and the findings revealed that CuO-NPs with tinier particle sizes degraded MB more than CuONRs.

Assessment of molluscicidal and larvicidal activities of CuO nanoparticles on Biomphalaria alexandrina snails

BackgroundSchistosomiasis is a major, but generally overlooked, tropical disease carried by snails of the genus Biomphalaria, which have a large distribution in Egypt. Control of the intermediate host snail is critical in limiting schistosomiasis spread. On the topic of snails’ management, nanotechnology has gained more interest.ResultsCopper oxide nanoparticles, characterised by transmission electron microscopy and X-ray diffraction, showed a single crystal structure with an average crystallite size around 40 nm by X-ray diffraction and typical transmission electron microscopy (TEM) image. Also, the UV–VIS spectrophotometer displayed a sharp absorption band of CuO NPs. Molluscicidal activity of copper oxide nanoparticles against B. alexandrina snails was observed. Following exposure to CuO NPs (LC50 and LC90 was 40 and 64.3 mg/l, respectively), there was a reduction in the growth and reproductive rates of treated B. alexandrina at the sub-lethal concentrations, as well as, a drop in egg viability. Moreover, CuO NPs exhibited a toxic effect on miracidiae and cercariae of S. mansoni. Scanning electron microscopy (SEM) investigations of the head-foot and mantle of control and treated snails to the sub-lethal concentrations of CuO NPs (LC10 15.6 mg\\l–LC25 27.18 mg\\l) indicated morphological alterations in the ultrastructure.ConclusionsCuO NPs caused a significant effect against the intermediate hosts of S. mansoni and provide a considerable scope in exploiting local indigenous resources as snail molluscicidal agents.

Determination of Antagonistic Effect of CuO NPs against Bacterial Cultures

Bacterial cultures are capable of causing infections to the humans. These bacteria can be killed or inhibited by special compounds called antibacterial agents. These compounds are generally not toxic to humans as most of the compounds are obtained from natural sources, such as, b-lactams (like penicillins), cephalosporins. Overuse of traditional antibacterial drugs, resistance may develop by bacterial cells, which ultimately leads to pose greatest health challenges by occurrence of infectious diseases. Therefore, development of non-resistance alternative antibacterial agents for better antibacterial efficacy is mandatory. This paper reports on the synthesis of copper oxide nanoparticles carried out by chemical precipitation method and explored its antibacterial efficacy against hospital borne bacterial infections. Copper oxide nanoparticles were synthesized by solvothermal route. The screening of antimicrobial activity of copper oxide nanoparticles was studied on the bacteria Staphylococcus aureus NCIM 2079 and Bacillus cereus NCIM 5293 by Anti Well Diffusion Assay (AWDA) on nutrient agar (NA) medium. It was evident that the Staphylococcus aureus was more sensitive for CuO NPs compared to Bacillus cereus. The synthesized CuO-NPs showed remarkable antibacterial activity against Bacillus cereus and Staphylococcus aureus. Minimum Inhibitory (MIC) and Minimum Bactericidal Concentration (MBC) of CuO-NPs were determined by calculating concentration dependent colony forming units per milliliter (CFU/mL) on agar plate. To synthesis of copper oxide nanoparticles carried out by chemical precipitation method and explored its antibacterial efficacy against hospital borne bacterial infections.

Green Synthesis, Characterization and Antibacterial Activity of Copper Oxide Nanoparticles Using Annona muricata Stem Extract

Abstract: Nanotechnology is a rising field of science and technology that deals with the particles having size in the range of 1 to 100 nm. Copper oxide nanoparticles has many properties like antifungal activity, antibacterial activity, optical properties, conductive properties, etc. Due to its demand of diversified use, copper oxide nanoparticles were fabricated using ecofriendly and non-toxic Annona muricata stem extract. The extract with copper sulphate pentahydrate showed gradual change in the colour of the extract from brown to green which indicates the CuO nanoparticles synthesis. The fabrication is followed by characterization of CuO nanoparticles using UV-vis spectroscope, FTIR, XRD and SEM. The characterization showed roughly spherical shaped nanoparticles in the range of 100nm with high crystalline monoclinic phase. FTIR absorption spectra conclude that the compounds attached with copper oxide nanoparticles could be polyphenols with aromatic ring. The CuO nanoparticles exhibited antibacterial activity; it showed the maximum activity against E.coli (18 mm). Keywords: Annona muricata, copper sulphate pentahydrate, FTIR, nanomaterials, SEM, XRD.

Green biosynthesis, structural characterization and anticancer activity of copper oxide nanoparticles from the brown alga Cystoseira myrica

Green biosynthesis, structural characterization and anticancer activity of copper oxide nanoparticles from the brown alga Cystoseira myrica

X ray diffraction (XRD) analysis and evaluation of antioxidant activity of copper oxide nanoparticles synthesized from leaf extract of Cissus vitiginea

Nanoparticles have a diameter of up to 100 nm and a higher surface-to-volume ratio, enabling more active surface atoms to contribute to implementations and improve material properties. In nanoparticle preparation, the ability to control particle size, shape, and morphology is important. The most important tool for studying nanomaterials is XRD, it is a vital characterization tool in solid-state chemistry and materials science. For any compound, XRD is a simple method for determining the unit cell's size and shape. This study explains how copper oxide nanoparticles are formed in Cissus vitiginea leaves. The antioxidant function and XRD study of the synthesized CuONPs were also investigated. According to the XRD results, the copper oxide nanoparticles formed by reducing Cu2 + ions by Cissus vitiginea leaf extract are crystalline in nature. CuONPs have an average crystalline size of ~32.32 nm, according to the Debye-Scherrer formula. CuONPs have higher antioxidant activity than plant extract and are closest to ascorbic acid in terms of standard.

Potential antidiabetic and anticancer activity of copper oxide nanoparticles synthesised using Areca catechu leaf extract

Herein we report the facile synthesis of copper oxide nanoparticles (CuO NPs) using Areca catechu leaf extract as a bio-reducing agent and capping agent via solution combustion synthesis. The formation, crystalline structure and morphology of as-prepared CuO NPs were confirmed using UV–visible spectroscopy, powder x-ray diffraction (XRD), energy dispersive x-ray spectroscopy (EDS), scanning electron microscopy (SEM), high resolution transmission electron microscopy (HR-TEM) and selected area electron diffraction (SAED) techniques. The powder XRD analysis confirmed the purity of the as-synthesised material and crystalline structure. SEM images revealed that the morphology of prepared CuO NPs is monoclinic phase in nature. This was further confirmed by observing TEM micrographs, which revealed the spherical nature of obtained material with an average size of particle between 10 and 25 nm. Further, the hypoglycaemic effect of CuO NPs was observed to be facilitated by impeding α-amylase, which also promoted glucose uptake by the yeast cells. In addition, CuO NPs showed significant antidiabetic activity. Obtained results suggest that the inhibition of α-amylase enzyme with IC50 value 260.33 μg ml−1 may be one of the feasible ways through which the CuO NPs exerts their hypoglycaemic effect. Furthermore, cytotoxic activity performed using CuO NPs against human lung cancer cell line (A549) proved that the prepared CuO NPs have significant anticancer activity with 8.84 μg ml−1 at 50% inhibition concentration (IC50 value).

Synthesis, characterization and antibacterial activity of copper oxide nanoparticles


 
 
 Copper oxide nanoparticles were synthesized by Chemical Precipitation Method using Copper Chloride Dihydrate (CuCl2. 2H2O), Sodium hydroxide (NaOH) as a precipitating agent. The Synthesized Copper Oxide nanoparticles were characterized by X-ray diffraction (XRD), Scanning Electron Microscope (SEM), Energy Dispersive X-ray (EDX) Spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR). The Antibacterial activity of copper Oxide nanoparticles was tested against both gram positive and negative bacteria. In XRD, the crystal size and dislocation density of Copper Oxide nanoparticles were calculated, Element’s purity was determined by EDX spectra. The SEM image confirms the presence of homogeneous spherical distribution of copper oxide nanoparticles. The nanoparticles shows interactions between copper and oxygen atoms were supported by FTIR studies. Copper Oxide nanoparticles have exhibits good antibacterial activity against Klebsiella pneumonia, Escherichia coli,Staphylococcus, and Bacillus cereus.
 
 

Antimicrobial and Biocompatible Polycaprolactone and Copper Oxide Nanoparticle Wound Dressings against Methicillin-Resistant Staphylococcus aureus.

One of the major health problems linked to methicillin-resistant Staphylococcus aureus (MRSA) is severe diabetic foot ulcers (DFU), which are associated with hospital-acquired infections, lower limb amputations and emerging resistance to the current antibiotics. As an alternative, this work aims to develop a biodegradable and biocompatible material with antimicrobial capacity to prevent DFU. This was achieved by producing active polymeric films with metallic nanoparticles dispersed through a polycaprolactone (PCL) dressing. First, the antimicrobial activity of copper oxide nanoparticles (CuONPs) was tested by the microdilution method, selecting the lowest concentration that has an inhibitory effect on MRSA. Then, active PCL films were prepared and characterized in terms of their physicochemical properties, antimicrobial performance, cytotoxicity, genotoxicity and hemocompatibility. Active films had chemical and thermal properties like the ones without the antimicrobial agents, which was confirmed through FTIR, Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) analysis. In relation to antimicrobial activity, active PCL films inhibited MRSA growth when treated with CuONPs at a concentration of 0.07% (w/w). After exposure to the active film extracts, human foreskin fibroblast cells (ATCC® SCRC1041™) (HFF-1) exhibited a cell viability average above 80% for all treatments and no DNA damage was found. Finally, PCL films with 0.07% (w/w) CuONPs proved to be hemocompatible, and none of the films evaluated had red blood cell breakage greater than 5%, being within the acceptable limits established by the International Organization for Standardization ISO 10993-4:2002.

The role of catalyst support on activity of copper oxide nanoparticles for reduction of 4-nitrophenol

Developing a facile and efficient method is an important approach for promising commercial catalytic applications. Toxic organic pollutants in waste waters are becoming a worldwide problem that threatens life on earth and prevents essential elements to sustain living organisms. Herein, the effect of catalyst support on the activity of copper oxide (CuO) nanoparticles for catalytic reduction of 4-nitrophenol (4-NP) in presence of sodium borohydride (NaBH4)-aqueous medium was discussed. A simple and conventional wet impregnation method was used for the deposition of CuO nanoparticles on several metal oxides (Al2O3, SiO2, MgO, CaO, ZnO, ZrO2). The prepared catalysts were characterized via using techniques such as X-ray diffraction (XRD), nitrogen sorption, inductively coupled plasma-optical emission spectrometry (ICP-OES), scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM/EDS), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). The effect of support material on the catalytic performance of CuO nanoparticles for the reduction of 4-NP was evaluated and the performance order of support was ZrO2 > Al2O3 > SiO2 > CaO > MgO > ZnO. CuO/ZrO2 catalyst exhibited excellent catalytic activity and the pseudo-first-order rate constant was determined as 15.97 · 10−3 s−1. Considering the simple preparation process and the efficient catalytic reduction of 4-NP of CuO/ZrO2, this study opens up a marvelous chance to this material for practical application of waste water treatment.

Proficient Photocatalytic and Sonocatalytic Degradation of Organic Pollutants Using CuO Nanoparticles

In recent years, due to the advancement in nanotechnology, advanced oxidation processes (AOPs), especially sonocatalysis and photocatalysis, have become a topic of interest for the elimination of pollutants from contaminated water. In the research work reported here, an attempt has been made to study and establish a physicochemical mechanism for the catalytic activity of copper oxide nanoparticles (CuO NPs) in AOPs using the degradation of dyes as model contaminants. CuO NPs exhibited brilliant sonocatalytic and photocatalytic activities for the degradation of a cationic dye (Victoria Blue) as well as an anionic dye (Direct Red 81). The degradation efficiency of CuO NPs was calculated by analysing the variation in the absorbance of dye under a UV-Vis spectrophotometer. The influence of different operating parameters on the catalytic activity of CuO NPs, such as the amount of catalysts dose, pH of the solution, and the initial dye concentration, was thoroughly investigated. In addition, the kinetic process for the degradation was also examined. It was observed that both dyes exhibited and followed the pseudo-first-order kinetics relation. The rate constant for sonocatalysis was high as compared to photocatalysis. The rate constant for both sonocatalysis and photocatalysis was successfully established, and reusability tests were done to ensure the stability of the used catalysts. To get an insight into the degradation mechanism, experiments were performed by using ⋅OH radical scavengers. The efficacy of CuO NPs for dye decolorization was found to be superior for the sonocatalyst than the photocatalyst.

Biosynthesis of Copper Oxide Nanoparticles with Potential Biomedical Applications.

IntroductionIn recent years, the use of cost-effective, multifunctional, environmentally friendly and simple prepared nanomaterials/nanoparticles have been emerged considerably. In this manner, different synthesizing methods were reported and optimized, but there is still lack of a comprehensive method with multifunctional properties.Materials and MethodsIn this study, we aim to synthesis the copper oxide nanoparticles using Achillea millefolium leaf extracts for the first time. Catalytic activity was investigated by in situ azide alkyne cycloaddition click and also A3 coupling reaction, and optimized in terms of temperature, solvent, and time of the reaction. Furthermore, the photocatalytic activity of the synthesized nanoparticles was screened in terms of degradation methylene blue dye. Biological activity of the synthesized nanoparticles was evaluated in terms of antibacterial and anti-fungal assessments against Staphylococcus aureus, M. tuberculosis, E. coli, K. pneumoniae, P. mirabili, C. diphtheriae and S. pyogenes bacteria’s and G. albicans, A. flavus, M. canis and G. glabrata fungus. In the next step, the biosynthesized CuO-NPs were screened by MTT and NTU assays.ResultsBased on our knowledge, this is a comprehensive study on the catalytic and biological activity of copper oxide nanoparticles synthesizing from Achillea millefolium, which presents great and significant results (in both catalytic and biological activities) based on a simple and green procedure.ConclusionComprehensive biomedical and catalytic investigation of the biosynthesized CuO-NPs showed the mentioned method leads to synthesis of more eco-friendly nanoparticles. The in vitro studies showed promising and considerable results, and due to the great stability of these nanoparticles in a green media, effective biological activity considered as an advantageous.