Characterization And Antimicrobial Activity of Copper Oxide Nanoparticles Synthesized by Crocus Sativus Extract

Abstract

This study aimed to evaluate the antimicrobial activity of green synthesized copper-oxide nanoparticles (CuO-NPs) using Crocus sativus. The microorganisms were supplied from Baghdad Teaching Hospital. Using C. sativus, CuO nanoparticles were synthesized in a green manner, and their properties were assessed using UV-visible spectroscopy at the peak of 260 nm. Atomic force microscopy (AFM) was used to measure the particles' size and form, and x-ray technology was used to more precisely quantify their dimensions. diffraction (XRD). The biomolecule and functional group were determined by Fourier transform infrared spectroscopy (FTIR). Different concentrations of nanoparticles (50, 75, and 100 ?g/ml) were subjected to the well diffusion method for antibacterial activity testing. The findings indicated that the CuO-NPs had a spherical form and a 0.154 nm size. The average diameters of the AFM pictures were found to be 11.89 nm, 2.62 nm, and 24.55 nm. The following growth zone inhibition values for the various bacteria species used were revealed by the nanoparticles' antimicrobial activity results: Staphylococcus aureus 18 mm, Staphylococcus epidermidis 18 mm, Klebsiella ssp 15 mm, Candida albican 18 mm, and Escherichia coli 17mm.