Determination of Antagonistic Effect of CuO NPs against Bacterial Cultures

Abstract

Bacterial cultures are capable of causing infections to the humans. These bacteria can be killed or inhibited by special compounds called antibacterial agents. These compounds are generally not toxic to humans as most of the compounds are obtained from natural sources, such as, b-lactams (like penicillins), cephalosporins. Overuse of traditional antibacterial drugs, resistance may develop by bacterial cells, which ultimately leads to pose greatest health challenges by occurrence of infectious diseases. Therefore, development of non-resistance alternative antibacterial agents for better antibacterial efficacy is mandatory. This paper reports on the synthesis of copper oxide nanoparticles carried out by chemical precipitation method and explored its antibacterial efficacy against hospital borne bacterial infections. Copper oxide nanoparticles were synthesized by solvothermal route. The screening of antimicrobial activity of copper oxide nanoparticles was studied on the bacteria Staphylococcus aureus NCIM 2079 and Bacillus cereus NCIM 5293 by Anti Well Diffusion Assay (AWDA) on nutrient agar (NA) medium. It was evident that the Staphylococcus aureus was more sensitive for CuO NPs compared to Bacillus cereus. The synthesized CuO-NPs showed remarkable antibacterial activity against Bacillus cereus and Staphylococcus aureus. Minimum Inhibitory (MIC) and Minimum Bactericidal Concentration (MBC) of CuO-NPs were determined by calculating concentration dependent colony forming units per milliliter (CFU/mL) on agar plate. To synthesis of copper oxide nanoparticles carried out by chemical precipitation method and explored its antibacterial efficacy against hospital borne bacterial infections.