Species Differences In Activity Research Articles

Analysis of antioxidants in water striders (Hemiptera: Gerridae) as bioindicator of water pollution.

The antioxidant enzyme system is an important defense mechanism to cope with Reactive Oxygen Species (ROS) produced due to exposure to heavy metals. In the present study lead (Pb), chromium (Cr), arsenic (As), cadmium (Cd) and nickel (Ni) in water and the antioxidant activity of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) was investigated in three species (Metrocoris communis, Limnogonus fossarum fossarum, and Aquarius adelaidis) of water striders collected from the industrial triangle of Punjab, Pakistan. The results of present study revealed that Pb, Cr, As, Cd and Ni were according to the permissible amount of WHO. The antioxidant activity of SOD, CAT and POD was found significantly different among species against oxidative stress, but found the highest activity of determining parameters in A. adelaidis. This is one of the pioneer studies in Pakistan reporting the role of water striders as a bioindicator of heavy metals present in the water through antioxidants enzyme variations. The current results supported that variant level of antioxidant enzyme activities in different species of water strider were reflective of heavy metal pollution in the Industrial triangle of Punjab, Pakistan and will be a useful ecotoxicological tools to evaluate the detrimental effects of heavy metal pollutants in aquatic organisms.

In Vitro Evaluation of Ferutinin Rich-Ferula communis L., ssp. glauca, Root Extract on Doxorubicin-Induced Cardiotoxicity: Antioxidant Properties and Cell Cycle Modulation.

The clinical use of anthracycline Doxorubicin as an antineoplastic drug in cancer therapy is limited by cardiotoxic effects that can lead to congestive heart failure. Recent studies have shown several promising activities of different species of the genus Ferula belonging to the Apiaceae Family. Ferula communis is the main source of Ferutinin-a bioactive compound isolated from many species of Ferula-studied both in vitro and in vivo because of their different effects, such as estrogenic, antioxidant, anti-inflammatory, and also antiproliferative and cytotoxic activity, performed in a dose-dependent and cell-dependent way. However, the potential protective role of Ferutinin in myocardium impairment, caused by chemotherapeutic drugs, still represents an unexplored field. The aim of this study was to test the effects of Ferutinin rich-Ferula communis L. root extract (FcFE) at different concentrations on H9C2 cells. Moreover, we evaluated its antioxidant properties in cardiomyocytes in order to explore new potential therapeutic activities never examined before in other experimental works. FcFE, at a concentration of 0.25 µM, in the H9C2 line, significantly reduced the ROS production induced by H2O2 (50 µM and 250 µM) and traced the cell mortality of the H9C2 co-treated with Ferutinin 0.25 µM and Doxorubicin (0.5 µM and 1 µM) to control levels. These results showed that FcFE could protect against Doxorubicin-induced cardiotoxicity. Further molecular characterization of this natural compound may open the way for testing FcFE at low concentrations in vivo and in clinical studies as an adjuvant in cancer therapy in association with anthracyclines to prevent side effects on heart cells.

Use of Pitzer’s model to calculate thermodynamic properties of aqueous electrolyte solutions of sulfuric acid

Over this study we calculated the coefficients of activity of different species and the concentration in the presence of H+ ion when H2SO4 is alone in solution. The coefficient of average activity was calculated using the model of Pitzer. The constants of dissociation of these reactions were calculated from the values of the free standard energies. The reaction reaches a balance while producing H+, HSO4- and SO42-. The research reported here concentrated on the effect of some important operational parameters on dissolution process. The parameters were investigated and their ranges are as follows: initial molality ranging from 0.001 to 5 mol.kg-1 and temperatures between 25 and 200°C. Fortran 90 (Mathematical formula translating system) was used to perform all mathematical calculations. The numerical code can be used to calculate the molarity of ion H+ of an aqueous solution of sulfuric acid. The objective of the present work is to optimize the parameters of leaching such as activity of sulfuric acid on the dissociation phenomena.

Structural insights of Labeo catla (catla) myxovirus resistance protein,GTP binding recognition and constitutive expression induced with Poly I:C

The Myxovirus resistance (Mx) proteins are critical effectors belonging to the super-family of guanidine triphosphatase, often stimulated by type I interferon (IFN) and mediates antiviral responses to restrict the replication of numerous viral genes in fishes. In teleosts, Mx proteins display diverse and complicated antiviral activity in different species. The present investigation seeks to characterize the Mx gene from Labeo catla upon induction by double-stranded (ds) RNA, polyinosinic-polycytidylic acid, (poly I: C). Molecular modeling and all-atoms molecular dynamics (MD) simulations were employed to understand the architecture of the GTPase domain and its plausible mode of GTP recognition in Mx protein. The full-length L. catla Mx (LcMx) gene sequence (1821 bp nucleotides) encodes an open reading frame of 606 amino acids. Domain search indicated conserved tripartite domain architecture of LcMx and forms a major cluster with the Mx from other teleosts. The positively charged Arginine and polar Glutamine residues from helix 3 and 4 of stalk region LcMx aid in homo-oligomerization. MD simulation portrayed the role of conserved critical residues aid in GTP recognition by the GTPase domain which perfectly corroborates with experimental findings and prior MD studies. After injection of poly I:C, the temporal mRNA profile showed that LcMx expression was significantly elevated in the spleen, brain, kidney, liver, muscle, heart, intestine, and gill tissues. Collectively, these results suggest that the elevated expression of the major innate immune defense gene Mx was able to inhibit the poly I: C mediated virulence in fish. Communicated by Ramaswamy H. Sarma

P-037 Zinc oxide/collagen (ZnOC) biocomposites for the development of novel meshes

Abstract When we talk about abdominal wall hernias, we are talking about a complex surgical approach. In recent years there is a special interest in the use and development of meshes with antimicrobial properties. Doctors are making efforts to continue progress in this area. We propose the development of meshes covered with Zinc Oxide in a collagen matrix. They have biomedical applications and antimicrobial properties. The objectives of the study are to highlight the major contributions that Zinc Oxide in collagen matrices could make, in the development of new antimicrobial properties. The research will test both the synthesis method of Zinc Oxide in collagen matrix, as well as its ability to inhibit the bacterial activity of different species of bacteria. We demonstrate the significant contributions that could be made by using composites based on Zinc Oxide in collagen matrices for the development of new biocompatible medical devices with antimicrobial properties. This will lead to important advances in the development of new networks based on Zinc Oxide in collagen matrix (ZnOC). Physicochemical characterization of ZnOC composites was performed, using appropriate techniques such as X-ray diffraction (XRD), scanning electron microscopy (SEM and EDS) and transmission electron microscopy (TEM), infrared spectroscopy (FTIR). The antimicrobial properties of ZnOC biocomposites was investigated using clinical and reference microorganisms belonging to Gram positive, Gram negative and fungal families.

Antibacterial and Antibiofilm Activity of Different Species of Fabiana sp. Extract Obtained via Maceration and Ultrasound-Assisted Extraction against Staphylococcus epidermidis.

Staphylococcus epidermidis is an opportunistic pathogen that, under certain conditions, can induce aggravated infectious processes, mainly in immunosuppressed patients. Moreover, S. epidermidis is one of the leading causes of medical device- and implant-associated infections and is also recognized as a canonical biofilm producer. Fabiana punensis, F. densa and F. patagonica are three medicinal plants that grow in arid environments in Argentina (Altoandina, Puna, Prepuna and Monte regions). In this work, we studied the antimicrobial activity of alcoholic extracts of these plant species obtained via maceration (M) and ultrasound-assisted extraction (UAE) against S. epidermidis. In addition, the antibiofilm activity of the F. densa extract was also evaluated. It was found that the extracts obtained via M did not present differences with those obtained via UAE regarding the chemical profile. F. densa showed the lowest minimum inhibitory concentration (MIC) value (75 µg GAE/mL). At concentrations higher than the MIC, the extract induced the release of cellular constituents. At the concentration of 1/8× MIC, the extract inhibited biofilm formation by 78%, reducing metabolic activity by 67%. On the other hand, it presented a low percentage of preformed biofilm removal. In all assays, gallic acid (GA) has been used as a reference antimicrobial compound. Finally, it was shown via microscopy visualization that the extract reduces adhesion to hydrophobic and hydrophilic surfaces. Thus, F. densa extracts could potentially be used for the antibiotic treatment of infections produced by S. epidermidis or as an inhibitor agent of production biofilm, avoiding infections caused by medical devices.

Identifying Bioactive Ingredients and Antioxidant Activities of Wild Sanghuangporus Species of Medicinal Fungi.

Sanghuangporus refers to a group of rare medicinal fungi with remarkable therapeutic properties. However, current knowledge on the bioactive ingredients and antioxidant activities of different species of this genus is limited. In this study, a total of 15 wild strains from 8 species of Sanghuangporus were selected as the experimental materials for identification of the bioactive components (polysaccharide, polyphenol, flavonoid, triterpenoid, and ascorbic acid) and antioxidant activities (scavenging activities against hydroxyl, superoxide, DPPH, and ABTS radicals; superoxide dismutase activity; and ferric reducing ability of plasma). Notably, individual strains contained different levels of various indicators, among which Sanghuangporus baumii Cui 3573, S. sanghuang Cui 14419 and Cui 14441, S. vaninii Dai 9061, and S. zonatus Dai 10841 displayed the strongest activities. The correlation analysis of bioactive ingredients and antioxidant activities revealed that the antioxidant capacity of Sanghuangporus is mainly associated with the contents of flavonoid and ascorbic acid, followed by polyphenol and triterpenoid, and finally, polysaccharide. Together, the results obtained from the comprehensive and systematic comparative analyses contribute further potential resources and critical guidance for the separation, purification, and further development and utilization of bioactive agents from wild Sanghuangporus species, as well as the optimization of their artificial cultivation conditions.

Species differences in activation of TRPA1 by resin additive-related chemicals relevant to indoor air quality.

Transient Receptor Potential Ankyrin 1 (TRPA1), which is expressed in the airways, has causative and exacerbating roles in respiratory diseases. TRPA1 is known as a target of sick building syndrome-related air pollutants, such as formaldehyde. Thus, an in vitro TRPA1 activation assay would be useful for predicting the potential risk of air pollution. In this study, we used human TRPA1 (hTRPA1)- and mouse TRPA1 (mTRPA1)-expressing cell lines to measure TRPA1 activation by the emerging indoor air pollutants 2-ethyl-1-hexanol (2-EH), a mixture of 2,2,4-trimethyl-1,3-pentanediol 1- and 3-monoisobutyrate (Texanol), and 2,2,4-trimethyl-1,3-pentanediol diisobutyrate (TXIB). The results indicated that 2-EH activated both hTRPA1 and mTRPA1 in a concentration-dependent manner, whereas TXIB did not activate hTRPA1 or mTRPA1. Texanol also activated hTRPA1 in a concentration-dependent manner. In contrast, a bell-shaped concentration-dependent curve was observed for mouse TRPA1 activation by Texanol, indicating inhibitory effects at a higher concentration range, which was also reported for menthol, a typical TRPA1 modulator. To further elucidate the mechanism underlying the species difference in TRPA1 activation by Texanol, V875G and G878V mutations were introduced into hTRPA1 and mTRPA1, respectively, which were reported to be key mutations for the inhibitory effect of menthol. These mutations switched the inhibitory effects of Texanol; thus, hTRPA1/V875G, but not mTRPA1/G878V, was inhibited at higher concentrations of Texanol. These results indicate that Texanol shares an interaction site with menthol. Overall, these findings suggest that careful interpretation is necessary when extrapolating rodent TRPA1-dependent toxicological effects to humans, especially with respect to the risk assessment of indoor air pollutants.

Proteomic Response of Paracoccidioides brasiliensis Exposed to the Antifungal 4-Methoxynaphthalene-N-acylhydrazone Reveals Alteration in Metabolism.

Background: Paracoccidioidomycosis is a neglected mycosis with a high socioeconomic impact that requires long-term treatment with antifungals that have limitations in their use. The development of antifungals targeting essential proteins that are present exclusively in the fungus points to a potentially promising treatment. Methods: The inhibitor of the enzyme homoserine dehydrogenase drove the synthesis of N'-(2-hydroxybenzylidene)-4-methoxy-1-naphthohydrazide (AOS). This compound was evaluated for its antifungal activity in different species of Paracoccidioides and the consequent alteration in the proteomic profile of Paracoccidioides brasiliensis. Results: The compound showed a minimal inhibitory concentration ranging from 0.75 to 6.9 μM with a fungicidal effect on Paracoccidioides spp. and high selectivity index. AOS differentially regulated proteins related to glycolysis, TCA, the glyoxylate cycle, the urea cycle and amino acid metabolism, including homoserine dehydrogenase. In addition, P. brasiliensis inhibited protein synthesis and stimulated reactive oxygen species in the presence of AOS. Conclusions: AOS is a promising antifungal agent for the treatment of PCM, targeting important metabolic processes of the fungus.

Season, basal media and plant growth regulators effect in wood plant in vitro propagation: a comprehensive review

Wood plant in-vitro propagation is currently widely used and is not limited to the preparation of superior seeds but has been widely utilized for the production of phytopharmaceuticals and the production of a valuable and specific tree for the benefit of man. Studies focused on the influence of explant collection seasons, selection of basic media, and plant growth hormone. The results of the study showed the magnitude of the variables that must be considered in the propagation of tissue cultures even in the same species and types of cultures. The period of January and March is the best time for explant retrieval because it provides the best explant response in bud breakage, lowest browning, and best bud induction. The basic media of MS (Murashige & Skoog), as well as its various modifications and the basic medium of WPM (Wood Plant Medium), tend to be widely chosen as the basic media at various levels of subcultures in many woody plant species. The application of growth regulators shows a tendency to use BAP (benzyl amino purine) and NAA (naphthalene acetic acid) whose application is separately or in combination with a certain concentration according to the species and subculture phase. Other plant growth regulators (PGRs) that are also quite widely used are the IBA (indole butyric acid), IAA (indole acetic acid), and G3 (gibberellic acid) groups which are used in the multiplication and rooting stages. The information from the study can be used as basic information at the stage of optimizing tissue culture activities in different species, explanatory, and regeneration types. The success of the optimization stage will be used as an improvement to the previous woody plant tissue culture protocol so that optimization of success is achieved. In vitro propagation is always associated with the number of resources used, so efforts to improve in vitro protocols are a very important activity to evaluate that will lead to increased effectiveness to reduce resource inputs, and be able to realize low-cost wood plant tissue culture propagation.

Evaluating the antioxidant activity of different species using a novel reagentless chronopotentiometric method

Antioxidants are substances that even in low concentrations can delay or prevent unwanted oxidizing processes. In this way, they can prevent or treat certain diseases in a physiological environment. The presence, amount, concentration and activity of these compounds in foods, beverages and in living tissues are therefore important parameters. Many methods have been developed for assessing antioxidant activity or capacity in different samples.Recently we introduced a new, reagentless, chronopotentiometric method for antioxidant measurement that was tested by measuring L-ascorbate in aqueous samples. The method uses a carbon working electrode chemically modified by a thin reversible redox mediator layer on its measuring surface. A simple, two-step procedure is used for the determination. First, in a short, controlled potential step, the mediator layer is converted into its oxidized state. In the second step, the potential control is terminated and the open cell redox potential value is recorded over time to obtain an analytical signal.In this paper, results obtained in further experiments using the novel method are described. An electrochemically formed graphene docking layer was employed to increase the stability of the redox mediator layer. The performance of the method has been tested using different antioxidant species. The influence of redox layer thickness on the sensitivity has been studied. The expected shapes of the chronopotentiometric function (E–t) and the calibration curve (ΔE/Δtinitial–canalyte) were predicted using a rough model. The calculated and measured curves were compared.

Antioxidant activity of Patanwadi breed sheep milk over cow milk— A preliminary study

Milk is an excellent source of dietary antioxidants, however, the antioxidants activity of different species of milk varies significantly. Sheep milk’s compositional and functional properties are one of the untapped areas for Indian researchers. This present study evaluated and compared the antioxidant potential of Patanwadi breed sheep milk over cow milk. The protein, fat, and lactose content of Patanwadi breed sheep milk was significantly higher than cow’s milk. Different anti-oxidant assays confirmed that the antioxidant activity of sheep milk was superior to cow milk. The present study supports that Patanwadi breed sheep could be a potential source of dietary antioxidants. Therefore, more scientific studies are required to develop sheep milk functional dairy products, and thereof optimized process parameters for sheep milk dairy products in Indian conditions.

Amplitude of the daily pattern of rest – activity in different species of Leopardus kept in captivity

Abstract Closer examination of the diurnal or nocturnal nature of wildlife species improves the knowledge necessary for landscape identity and biodiversity preservation. The aim of this study was to evaluate the daily rhythmicity of total locomotor activity in wild felids of several species of Leopardus of similar body weight housed in captivity: Geoffroy’s cat (Leopardus geoffroyi), ocelot (Leopardus pardalis), oncilla (Leopardus tigrinus) and margay (Leopardus wiedii). Twenty-four felids, six animals for each species, were housed under a natural light/dark cycle. The activity was recorded for thirteen consecutive days by means of an actimeter attached to a neck collar. Using cosinor rhythmometry, circadian rhythmic parameters (mesor, amplitude and acrophase) were assessed and compared among the several species. The daily and individual chronobiological variations of rest and activity showed a well-defined pattern. A nocturnal daily rhythmicity of locomotor activity was observed in Geoffroy’s cat, ocelot, oncilla and margay. The acrophase was observed shortly after midnight in margay and Geoffroy’s cat, and early at night in oncilla and ocelot. Our results improve the knowledge about the circadian system in wild animals. They can be a contribution to understanding the adaptive behaviour of wild felid species kept in zoological parks and rehabilitation agencies in providing the proper care for these animals

Anti-inflammatory, anti-oxidant, and immunomodulatory activities of the genus Ferula and their constituents: A review.

Ferula is a genus of the family Apiaceae and it includes around 170 species of flowering plants mostly native to the Mediterranean region and eastern to central Asia. In Iran, Ferula spp. are widely used in cuisine and traditional medicine. This review discusses the anti-inflammatory, anti-oxidant, and immunomodulatory activities of different species of Ferula. To prepare the present review, Scopus, Google Scholar, PubMed, and Web of Science scientific databases were searched to retrieve relevant articles published from 1985 until December 2020. Based on our literature review, Ferula plants and their derivatives decrease the levels of inflammatory mediators and exert anti-apoptotic effects. Under oxidative stress conditions, these plants and their constituents were shown to decrease oxidative markers such as malondialdehyde, reactive oxygen species, and nitric oxide but increase superoxide dismutase, glutathione peroxidase, catalase activity, and glutathione level. Ferula plants and their constituents also showed immunomodulatory effects by affecting various cytokines. Besides, in vivo and in vitro studies showed hypotensive, neuroprotective, memory-enhancing, anti-oxidant, hepatoprotective, antimicrobial, anticarcinogenic, anticytotoxic, antiobesity, and anthelmintic effects for various species of Ferula and their constituents. These plants also showed a healing effect on gynecological issues such as miscarriage, unusual pain, difficult menstruation, and leukorrhea. All these beneficial effects could have resulted from the anti-inflammatory, anti-oxidant, and immunomodulatory effects of these plants and their constituents. Based on the available literature, members of the genus Ferula can be regarded as potential therapeutics against inflammatory conditions, oxidative stress, and immune dysregulation.

Waste combustion and lime induced calcite precipitation for problematic soils stabilization; an applied review

The precipitation of calcite for sustainable modification of expansive soils for green and sustainable construction has been a subject of serious energy investigation in the past years. This is due to the promise for an eco-friendly construction materials synthesis and efficient soil stabilization and earthworks. Results of previous studies have shown remarkable achievement in this field in recent years. These range between treated soils swelling potential mitigation, shrinkage and crack remediation and strength characteristics improvement. It is very important to note that these results were achieved by deploying the activities of different species of bacteria in a mechanism known as microbial induced calcite precipitation (MICP). However, the affinity between lime (CaO) and CO2 emission; released during the direct and controlled combustion of solid waste in the derivation of ash is proposed in this review work. It is proposed to precipitate calcite in another mechanism and setup known as “lime induced calcite precipitation” (LICP). In order words, this is a complete energy cycle of emission entrapment and sustainable generation of construction materials (ash). This review has proposed that calcite can be precipitated without going through the rigors of securing bacteria and adopting the hydrolysis processes. Generally, this simple setup proposed in this review work serves the same energy purpose of inducing the precipitation of calcite as a construction geomaterial.

Harnessing the Potential of Roots of Traditional Power Plant: Ocimum.

Genus Ocimum of Labiatae is well known in all traditional medicinal systems like Ayurveda, Unani, Siddha, and Homeopathy. The pharmaceutical activities of different species of Ocimum attributed to all plant parts. Roots are the most significant vital organ of the plant, as they absorb water and nutrients from soil and transport to aerial parts of the plants. Roots of Ocimum were found helpful with free-radical scavenging activity to improve physical and mental strength as well as to treat diabetes, malaria, and liver problems. Antibacterial activity of Ocimum roots and its main component, rosmarinic acid, is very beneficial to protect against several human pathogens, including bacteria and viruses. Being so important in every way, roots of Ocimum need healthy rhizosphere. Bacteria, fungi, nematodes, types of soil, fungicide, pesticides, salt, radioactive elements, as well as heavy metal contaminations, affect roots and overall growth of Ocimum in positive or negative ways. Each component of rhizosphere (natural, treatment or contamination) affects the roots, which highlights current ecological scenario to discover biosafe and more productive approaches. For such prestigious organ of Ocimum, development of in vitro root cultures and hairy root cultures assists to reduce the efforts and timing of the traditional cultivation process along with elimination of negative factors in rhizosphere. Different strains of Agrobacterium rhizogenes, various media compositions, as well as discrete treatments, like elicitors, on nonidentical species or cultivars of Ocimum boost the root induction, biomass, and accumulation of phytoceuticals differently. Hairy roots and in vitro roots of Ocimum accumulate higher quantity of therapeutic metabolites. These metabolites include several phenolics (like rosmarinic acid, 3-hydroxybenzoic acid, m-coumaric acid, p-coumaric acid, caffeic acid, ferulic acid, vanillic acid, chicoric acid, and lithospermic acid), triterpenes (such as betulinic acid, 3-epimaslinic acid, alphitolic acid, euscaphic acids, oleanolic acid, and ursolic acid) as well as flavonoids (flavones, flavonols, and dihydroflavonols). This review highlights pharmaceutical applications of Ocimum roots, a great deal of rhizosphere components and in vitro culturing techniques to enhance biomass as well as chief phytoceuticals.

Chemical composition and antioxidant activity of three species of Cornukaempferia in Thailand

Abstract. Chumroenphat T, Saensouk S, Saensouk P. 2021. Chemical composition and antioxidant activity of three species of Cornukaempferia in Thailand. Biodiversitas 22: 4036-4044. Genus Cornukaempferia (Zingiberaceae) were used as medicinal plants in Thailand. Cornukaempferia has never been reported the chemical compounds and antioxidant activity before. This study was designed to identify the chemical composition and antioxidant activities in root, rhizome, stem, and leaves of Cornukaempferia aurantiflora, C. larsenii, and C. longipetiolata. DPPH scavenging activity and Ferric reducing antioxidant power (FRAP) assays assessed the chemical composition and antioxidant activity. The results showed that total phenolic content (TPC) and total flavonoid content (TFC) found different plant parts obtained in this study were in the order of root>rhizomes>leaves>?stems. The C. aurantiflora had a high TPC of 416.39 mg GAE/g dry weight, while individual phenolics, such as vanillic acid at 256.90 mg/g dry weight, showed high contents in the leaf part. For C. larsenii, TFC and individual flavonoids, such as catechin, have high 62.52 and 56.47 mg/100g dry weight values in the roots, respectively. The antioxidant activities in different species of different plant parts (DPPH: root>leaves?> rhizomes?>?stems; FRAP: leaves?>root>?rhizomes?>?stems). The genus Cornukaempferia is a rich source of bioactive compounds and antioxidant activity. Therefore, the genus Cornukaempferia will be used as medicine and food to produce products with health benefits in the future.

Comparative analysis of phytochemicals, antimicrobial and antioxidant activity of different species of Terminalia from Himachal Pradesh, India

Comparative analysis of phytochemicals, antimicrobial and antioxidant activity of different species of Terminalia from Himachal Pradesh, India

Iterative tomography with digital adaptive optics permits hour-long intravital observation of 3D subcellular dynamics at millisecond scale

Long-term subcellular intravital imaging in mammals is vital to study diverse intercellular behaviors and organelle functions during native physiological processes. However, optical heterogeneity, tissue opacity, and phototoxicity pose great challenges. Here, we propose a computational imaging framework, termed digital adaptive optics scanning light-field mutual iterative tomography (DAOSLIMIT), featuring high-speed, high-resolution 3D imaging, tiled wavefront correction, and low phototoxicity with a compact system. By tomographic imaging of the entire volume simultaneously, we obtained volumetric imaging across 225× 225× 16μm3, with a resolution of up to 220nm laterally and 400nm axially, at the millisecond scale, over hundreds of thousands of time points. To establish the capabilities, we investigated large-scale cell migration and neural activities in different species and observed various subcellular dynamics in mammals during neutrophil migration and tumor cell circulation.

An Overview of Metabolic Activity, Beneficial and Pathogenic Aspects of Burkholderia Spp.

Burkholderia is an important bacterial species which has different beneficial effects, such as promoting the plant growth, including rhizosphere competence for the secretion of allelochemicals, production of antibiotics, and siderophores. In addition, most of Burkholderia species have demonstrated promising biocontrol action against different phytopathogens for diverse crops. In particular, Burkholderia demonstrates significant biotechnological potential as a source of novel antibiotics and bioactive secondary metabolites. The current review is concerned with Burkholderia spp. covering the following aspects: discovering, classification, distribution, plant growth promoting effect, and antimicrobial activity of different species of Burkholderia, shedding light on the most important secondary metabolites, their pathogenic effects, and biochemical characterization of some important species of Burkholderia, such as B. cepacia, B. andropogonis, B. plantarii, B. rhizoxinica, B. glumae, B. caryophylli and B. gladioli.