According to the Ministry of Employment and Labor, 40% of fatal accidents due to industrial accidents between 2017 and 2021 were at partner workplaces, and 62.6% of all accidents (as of 2021) were preventable by following basic safety rules, such as falling, getting caught, or colliding. Therefore, this study investigated the effect of the prime companies’ safety support on the safety culture creation and safety behavior of workers in partner workplaces as a way to establish a safety culture in partner workplaces. This study was designed to examine the effect of safety support from prime companies on safety behavior level of workers at partner workplaces. It is a cross-sectional study using the Korea Occupational Safety and Health Agency’s 2021 Occupational Safety and Health data. The sample for the study is 739 manufacturing partner workplaces. Data were analyzed using descriptive statistics and hierarchical multiple regression analysis using SPSS (ver.21). According to this study, there were labor unions in 91 places and 13.9% in 94 places with departments dedicated to industrial safety and health, indicating that the establishment of an industrial safety and health system was generally insufficient. In addition, this study examined the effect of the prime companies' safety support on the safety culture of partner workplaces and the safety behavior of their workers. As a result of the hierarchical multiple regression analysis, safety support from prime companies had a positive (+) effect on safety culture at partner workplaces. Leadership of management appear to have a partial mediating effect on safety support from prime companies and safety behavior level at partner workplaces. Safety communication and education·systems appear to have a mediating effect on safety support from prime companies and safety behavior level at partner workplaces. According to this study, active safety support from the prime companies can revitalize the safety culture of the partner workplaces, thereby improving the level of safety behavior of workers to prevent accidents. Since partner workplaces generally lack safety capabilities compared to the prime companies, they can spread the safety culture of partner workplaces through safety support from the prime companies, and it can be expected to be effective in preventing industrial accidents by controlling the unsafe behavior of workers by establishing a safety culture.