Obesity is characterized as an excessive accumulation of fat in subcutaneous tissues. It is very influential for various diseases including cardiovascular disorders, type 2 diabetes. Adipose tissue is consisted of two types of cell, which are WATs and BATs. WAT principally stores lipid in the form of TGs, thereby acting as a repository of surplus energy in the body. On the other hands, BAT utilizes stored lipid for nonshivering thermogenesis, involving β‐oxidation and uncoupling of oxidative phosphorylation in mitochondria.Silk peptide and extracts of silkworm have diverse pharmacological activities, inducing hyperglycemia, hyperlipidemia, and anti‐oxidant effect. As well as, silk peptide decreases lipid metabolism and body fat accumulation. However adipose browning mechanism as an one of anti‐obesity effect has not been elucidated.In this research, silk peptides increases UCP1 expression and mitochondrial biogenesis in subcutaneous white adipose cells isolated from ICR mice. As well as, silk peptides induces BAT‐like differentiation of adipocytes and thermogenic activation, a process that also involves the upregulation of FAO. In conclusion, we suggest that silk peptides‐induced UCP1 activation in adipocytes have prospective therapeutic value for obesity suppression and associated metabolic disease.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.