Activated Sludge Research Articles

Machine learning modeling for the prediction of phosphorus and nitrogen removal efficiency and screening of crucial microorganisms in wastewater treatment plants

The effectiveness of wastewater treatment plants (WWTPs) is largely determined by the microbial community structure in their activated sludge (AS). Interactions among microbial communities in AS systems and their indirect effects on water quality changes are crucial for WWTP performance. However, there is currently no quantitative method to evaluate the contribution of microorganisms to the operating efficiency of WWTPs. Traditional assessments of WWTP performance are limited by experimental conditions, methods, and other factors, resulting in increased costs and experimental pollutants. Therefore, an effective method is needed to predict WWTP efficiency based on AS community structure and quantitatively evaluate the contribution of microorganisms in the AS system. This study evaluated and compared microbial communities and water quality changes from WWTPs worldwide by meta-analysis of published high-throughput sequencing data. Six machine learning (ML) models were utilized to predict the efficiency of phosphorus and nitrogen removal in WWTPs; among them, XGBoost showed the highest prediction accuracy. Cross-entropy was used to screen the crucial microorganisms related to phosphorus and nitrogen removal efficiency, and the modeling confirmed the reasonableness of the results. Thirteen genera with nitrogen and phosphorus cycling pathways obtained from the screening were considered highly appropriate for the simultaneous removal of phosphorus and nitrogen. The results showed that the microbes Haliangium, Vicinamibacteraceae, Tolumonas, and SWB02 are potentially crucial for phosphorus and nitrogen removal, as they may be involved in the process of phosphorus and nitrogen removal in sewage treatment plants. Overall, these findings have deepened our understanding of the relationship between microbial community structure and performance of WWTPs, indicating that microbial data should play a critical role in the future design of sewage treatment plants. The ML model of this study can efficiently screen crucial microbes associated with WWTP system performance, and it is promising for the discovery of potential microbial metabolic pathways.

Internal driving mechanisms of microbial community and metabolism for granular activated carbon enhancing anaerobic digestion of high-strength sulfate organic wastewater

In this study, the feasibility of enhancing high-strength sulfate organic wastewater anaerobic digestion by adding granular activated carbon (GAC) and the internal driving mechanisms were investigated in up-flow anaerobic sludge blanket (UASB) reactors. The UASB reactor with GAC (GAC reactor) was more stable in COD removal efficiency and CH4 production than that without GAC (non-GAC reactor). When the influent COD concentration increased to 12,000 mg/L with a COD/SO42− ratio of 4.0, the COD removal efficiency and CH4 yield increased by 10.45 % and 20.50 %, respectively. Under inhibition condition with sulfides of 800 mg/L, specific methanogenic activity of GAC reactor sludge increased by 41.8 % compared to that of non-GAC reactor sludge. Electron transport system and microbial community results showed that GAC enhanced microbial activity and improved direct interspecies electron transfer (DIET) between potential electroactive microorganisms and Methanosaeta. Functional enzyme gene analysis demonstrated that GAC enhanced microbial metabolic activity, covering glycolysis, pyruvate metabolism, sulfate reduction, and methanogenesis. Therefore, the enrichment of key microorganisms, the promotion of DIET methanogenesis, and the improvement of functional metabolism activity may be the important reasons for GAC reactor sludge with higher methanogenic activity. Furthermore, synergistic promotion of sulfate-reducing metabolism and methanogenesis may be due to the establishment of potential syntrophic partnership between sulfate-reducing bacteria (Desulfovibrio) and Methanosaeta. This study deciphered internal driving mechanisms of microbial community and metabolism for GAC enhancing anaerobic digestion of high-strength sulfate organic wastewater.

Metatranscriptome revealed how carbon brush addition affected the fermentation of food wastewater in the low-temperature environment

Improving the anaerobic digestion (AD) performance in low-temperature environments has become a key factor in the development of waste treatment and resource recovery in cold regions. The utilization of external carriers to form a biofilm is the simplest and most practical way to enhance the psychrophilic AD performance in cold regions. In this study, the effect of carrier addition on the fermentation performance of low-temperature (15 ± 2 °C) food wastewater was investigated by forming biofilms with carbon brushes. The results showed that although the biofilm formation enhanced methane yields (15.24%), it also caused more accumulation of propionic acid (306.99–626.89 mg/L), and the concentration of acetic acid (86.78–254.71 mg/L) was relatively low. The microbial community revealed the highest abundance of the fermentative bacterium Firmicutes and the carbon brush carrier significantly increased its relative abundance (23.74%). Metatranscriptomic sequencing revealed that the abundance level of Clostridium, Bacteroides, Sedimentibacter and Pelotomaculum was the highest, reaching 80% in all groups. In addition, the abundance level of electroactive microorganisms in biofilms was higher, while the fermentation bacteria and methanogens were lower. This showed that biofilm can enrich more electroactive microorganisms, and granular sludge needs to enrich more fermentation bacteria and methanogens to ensure metabolic activity. Further studies have found that carbon metabolism had the highest activity (27.86%–30.39%) and H+-transporting ATPase (atp) was the most dominant functional enzyme (85.50%–86.65%) involved in electron transport in low-temperature fermentation of food wastewater. Interestingly, these expression levels of active granular sludge were higher than the biofilm formed by carbon brushes. Meanwhile, analysis of the methanogenic pathway found that active granular sludge tends to be directly metabolized to realize acetate to acetyl-CoA by acetyl-CoA synthetase (ACSS), while biofilms were not significantly different in the two metabolic pathways of acetate. These results deepen the understanding of treating low-temperature food wastewater.

Novel strategy to enhance the biological treatment of coal chemical wastewater by nano-zero valent ironloaded fly ash-based activated carbonassisted activated sludge process.

Industrial waste as novel conductive mediator was applied for wastewater treatment as a novel strategy for both waste recycling and sustainable development of wastewater treatment. In this study, nanoscale zero valent iron-loaded fly ash-based activated carbon (nZVI@FABAC) was prepared and applied to enhancing activated sludge (AS) process for coal chemical wastewater (CCW) treatment. The results demonstrated that the removal efficiencies of COD and total phenols (TPh) in nZVI@FABAC/AS process reached about 83.96 and 85.17%, which increased 52.51 and 31.52% compared with the single AS process, respectively. And the acute toxic unit value of CCW was reduced by 88.24% after nZVI@FABAC/AS process treatment. The various functional bacteria including phenol-degrading bacteria (Comamonas and Acinetobacter), electroactive bacteria (Geobacter), and iron reduction bacteria (Geothrix) were enriched in the nZVI@FABAC/AS process, which provided various electron transfer pathways to improve the degradation of toxic organics in CCW. Accordingly, nZVI@FABAC/AS process provided a promising and sustainable way for industrial wastewater treatment.

Bacterial communities in residential wastewater treatment plants are physiologically adapted to high concentrations of quaternary ammonium compounds

AbstractBenzalkonium chloride (BAC) is a quaternary ammonium compound (QAC) widely used as the active ingredient of disinfectants. Its excessive discharge into wastewater is constant and in high concentrations, likely affecting the physiology of microbial communities. We compared the physiological community profile of activated sludge (AS) bacteria with and without prior in vitro exposure to a high concentration of BAC (10 mg L−1). We measured the community functional diversity, carbon substrate multifunctionality, and the median effective concentration that inhibits carbon respiration (EC50) using Biolog EcoPlates supplemented with a gradient of 0–50 mg L−1 of BAC. Surprisingly, we did not find significant differences in the physiological parameters among treatments. Certain abundant bacteria, including Pseudomonas, could explain the community's tolerance to high concentrations of BAC. We suggest that bacterial communities in wastewater treatment plants’ AS are “naturally” adapted to BAC due to frequent and high‐dose exposure. We highlight the need to understand better the effects of QACs in wastewater, their impact on the selection of tolerant groups, and the alteration in community metabolic profiles.

Circular economy-based multi-objective decentralized controller for activated sludge wastewater treatment plant

Introduction: Water scarcity and water pollution are two major issues in India. Circular economy-based wastewater treatment technology provides the most sustainable solutions for solving these issues. In this paper, a novel multi-objective decentralized controller (MODC) is proposed for benchmarking a multi-input multi-output (MIMO) activated sludge wastewater treatment plant (WWTP) to achieve maximum effluent quality with minimum cost. WWTPs with conventional control schemes consume more energy to achieve the desired effluent quality.Methods: In this study, a MIMO model is developed for the activated sludge process (ASP) from a physics-based model, and relative gain array (RGA) analysis are carried out to determine the interaction between the loops to identify a suitable control scheme for the MIMO process. In addition, a multi-objective decentralized control problem is formulated to achieve the conflicting multiple objectives of improving effluent quality and minimizing operational costs by efficient usage of energy.Results and discussion: The desired quality and cost reduction are verified by comparing the integral square error (ISE) and control effort (CE) values of a closed-loop WWTP. A multi-objective evolutionary algorithm (MOEA), namely, the non-dominated sorting genetic algorithm (NSGA)-II, successfully solves the multi-objective control problem. NSGA-II provides several optimal solutions in the Pareto front. In order to demonstrate the feasibility of the proposed controller, three optimal solutions are selected from the Pareto-optimal front, and their closed-loop performances are evaluated qualitatively and quantitatively for both servo and regulatory operations. Improving the quality of effluent enhances active sludge production, which in turn increases the methane production in the anaerobic digester.

Synthetic vinasse treatment by combined UASB-ABR-AS system

Vinasse, or ethanol industry wastewater, is a major source of environmental pollution owing to its high levels of organic matter, toxic substances, and hard biodegradable compounds. Treatment of vinasse can reduce environmental pollutants significantly, and if well treated, it can be used as a fertilizer. This research aimed to evaluate the performance of a combined laboratory-scale system consisting of an upflow anaerobic sludge blanket (UASB) reactor and an anaerobic baffled reactor (ABR) followed by an activated sludge (AS) reactor in treating synthetic vinasse. The research was conducted for 208 days. The combined UASB-ABR-AS system was fed by synthetic vinasse under organic loading rate (OLR) between 2.5 and 13.5 kgCOD/m3.day. The COD removal efficiency rates of UASB, ABR, and AS units were obtained at 84%, 77.1%, and 90.7%, respectively. The overall COD removal efficiency rates of the combined UASB-ABR-AS system and methane production were 99.6% and nearly 72% (613.71 L/kgCOD), respectively. These results indicated that using the combined UASB-ABR-AS system is suitable for synthetic vinasse treatment and improves the effluent quality according to environmental standards.

Influencing factors on the activity of an enriched Nitrospira culture with granular morphology

ABSTRACT Nitrospira is a common genus of nitrite-oxidising bacteria (NOB) found in wastewater treatment plants (WWTPs). To identify the key factors influencing the composition of NOB communities, research was conducted using both sequencing batch reactor (SBR) and continuous flow reactor under different conditions. High-throughput 16S rRNA gene sequencing revealed that Nitrospira (18.79% in R1 and 25.77% in R3) was the dominant NOB under low dissolved oxygen (DO) and low nitrite ( NO 2 − -N) concentrations, while Nitrobacter (21.26% in R2) was the dominant NOB under high DO and high NO 2 − -N concentrations. Flocculent and granule sludge were cultivated with Nitrospira as the dominant genus. Compared to Nitrospira flocculent sludge, Nitrospira granule sludge had higher inhibition threshold concentrations for free ammonia (FA) and free nitrous acid (FNA). It was more likely to resist adverse environmental disturbances. Furthermore, the effects of environmental factors such as temperature, pH, and DO on the activity of Nitrospira granular sludge were also studied. The results showed that the optimum temperature and pH for Nitrospira granular sludge were 36°C and 7.0, respectively. Additionally, Nitrospira granular sludge showed a higher dissolved oxygen half-saturation constant (Ko) of 3.67 ± 0.71 mg/L due to its morphological characteristics. However, the majority of WWTPs conditions do not meet the conditions for the Nitrospira granular sludge. Thus, it can be speculated that future development of aerobic partial nitrification granular sludge may automatically eliminate the influence of Nitrospira. This study provides a theoretical basis for a deeper understanding of Nitrospira and the development of future water treatment processes.

Occurrence, distribution, and behaviors of erythromycin A, production byproducts, transformation products, and resistance genes in a full-scale erythromycin A production wastewater treatment system

Antibiotic production wastewater from pharmaceutical manufacturing is a significant source of antibiotic and resistance gene pollution in the environment. Given that Erythromycin A (Ery-A) is a widely used antibiotic in both human clinical and livestock breeding, it is imperative to ascertain its presence, along with related compounds, in the biological treatment processes of production wastewater. In this study, the occurrence and behavior of Ery-A, its production byproducts, transformation products, and resistance genes were first systematically investigated in a full-scale anaerobic-aerobic system for treating Ery-A production wastewater. Simultaneously, residual antibacterial activity in wastewater and sludge was evaluated throughout the wastewater treatment process. Ery-A contributes only 24.2 – 36.0% to the antibacterial activities. Ery-A-derived compounds including production byproducts (erythromycin B and erythromycin C) and transformation products (anhydro erythromycin A, N-demethyl-erythromycin A, and erythromycin A enol ether), are determined to contribute to the antibacterial activities of the wastewater treatment system. High concentrations of antibiotics with antibacterial activity (up to 1,258.9 mg/kg·TS for erythromycin A enol ether) adsorbed in the sludge result in near collapse of the first-stage anaerobic sludge system. Sludge biodegradation in second-stage anaerobic and anoxic-aerobic tanks is essential in removing Ery-A-related compounds from wastewater. The Ery-A-related compounds in the secondary effluent and excess sludge are determined to be 44.5 g/h and 1.5 g/h through the mass balance analysis, respectively. The discharge of MLS resistance genes from the secondary effluent and excess sludge is 1.0 × 1016 copies/h and 7.1 × 1015 copies/h, respectively. These findings highlight the significant concern over the release of Ery-A-related compounds and MLS resistance genes from the Ery-A production wastewater treatment system. As a result, it is crucial to implement strategies for the removal of Ery-A-related compounds from production wastewater before biological processes. This study is the first to report the occurrence and behavior of Ery-A-related compounds and resistance genes along the full-scale wastewater treatment processes. Additionally, it sheds light on the importance of byproducts and transformation products with antibacterial activity from Ery-A in the Ery-A production wastewater treatment system.

The neglected ammonia leaching calcium in anaerobic granular sludge

Previous researches have primarily emphasized the deleterious impacts of NH4+ on anaerobic granular sludge due to its biotoxicity. Despite this, the role of NH4+ as a monovalent cation in leaching multivalent Ca2+, thereby hindering granule formation and undermining its stability, remains underappreciated. This study investigated the potential of NH4+ to leach Ca2+ from anaerobic granular sludges. The results indicated that a shock loading of NH4+ at a concentration of 900 mg/L caused a Ca2+ leaching of 57.1 mg/L at pH 7.0. In an acidified environment (pH 5.0), the shock loading resulted in a Ca2+ release of 127.3 mg/L, a magnitude 5.24 times greater than the control group. The leaching process modestly affected granular sludge activity and size but markedly compromised granular strength due to calcium loss. Subsequent to the NH4+ shock, the granular strength manifested a significant reduction, as evidenced by a 15-fold increase in protein release from the granules compared to the intact ones. Additionally, NH4+ shock altered the calcium partitioning within the granular sludge, resulting in a decrease in residual calcium and a concomitant increase in bound calcium, further affecting granular strength. This study underscores the overlooked significant phenomenon of NH4+ shock-leaching Ca2+ in anaerobic granular sludge, which warrants significant attention given to its rapid and deleterious effects on granular strength and the shift in calcium state.

Wet dispersion of graphene assisted constructing multiple interface pores of PVDF hollow fiber membranes via melt-spinning and stretching

In this work, in order to further improve the permeability of poly(vinylidene fluoride) (PVDF) hollow fiber microfiltration membranes through melt-spinning and stretching (MS-S), we doped graphene (GE) in the forming membrane system of the previous work via wet dispersion and successfully prepared PVDF hollow fiber microfiltration membranes with multiple interface pores. GE could be uniformly dispersed and embedded in the PVDF matrix through the wet dispersion, which could obviously improve the interface layer between the matrix phase (PVDF) and the dispersed phase (GE) due to their poor compatibility of organic/inorganic phases. In addition, the stress concentration would be generated at the interface between GE and PVDF, which resulted in PVDF forking to many tiny fibers. Thus, the multiple interface pores between organic/organic (PVDF/polysulfone(PSf)) and organic/inorganic (PVDF/GE) phases could occur and be controlled precisely through the further stretching. The results showed that GE could improve the pore size and porosity of the prepared membranes obviously. In addition, the pore size of the prepared membranes could be controlled from 0.258 to 1.066 µm by changing the stretching ratios. Accordingly, pure water flux increased from 174.5 to 2782.9 L m-2 h-1. Applied to filter the active sludge suspension, the prepared PVDF microfiltration membranes exhibited a high rejection (＞99.5%), and the turbidity decreased obviously (˂0.5 NTU). In particular, there were not any hazardous solvents used in the preparation process, which could achieve green preparation of PVDF membranes.

Effect of hydraulic retention time on the performance of sequencing batch reactor under combined stress of ibuprofen and ciprofloxacin

The impacts of changes in hydraulic retention time (HRT) on nitrogen elimination, the activity of sludge and the structure of microbial communities in a sequencing batch reactor under the joint stress of ibuprofen (IBP) and ciprofloxacin (CIP) were investigated. Under the combined stress of IBP (belonged to nonsteroidal anti-inflammatory drugs) and CIP (belonged to antibiotics), the elimination level of ammonia nitrogen, chemical oxygen demand, and the rate of specific ammonia oxidation, specific nitrite oxidation, specific nitrate reduction in activated sludge declined with the shortening of HRT. Under the joint stress of IBP and CIP, the content of polysaccharide (PS) and protein (PN) together with the ratio of PN to PS in the extracellular polymeric substances grew with the shortening of HRT, and the content of aromatic proteins increased significantly, which indicated that aromatic proteins were the major protective response substances to the shortening of HRT. Under the joint stress of IBP and CIP, with the reduction of HRT, and the reduction rate of relative abundance of nitrifying bacteria was more evident than that of denitrifying bacteria. This exploration gives novel thoughts for understanding the impacts of HRT on biological nitrogen removal of wastewater under combined stress of IBP and CIP.

Evaluating source separation wastewater systems using traditional life cycle assessment and the planetary boundaries approach

Life cycle assessment (LCA) is a commonly used method for assessing environmental impacts of systems, but cannot produce absolute values, i.e. a comparison with existing calculated values, which represents limits of what can be emitted into the environment. Therefore, absolute environmental sustainability assessments have been developed to assess impacts against the planetary boundaries (PBs) of the safe operating space for humanity. Since PB-LCAs are novel, it is useful to analyze both results from this method and conventional LCAs, something which has not been done before. This study applied both methods to two full-scale sanitation systems in the city of Helsingborg, Sweden. The current conventional system for handling wastewater with active sludge and food waste to biogas production was compared with the novel project H+ source separation system with three pipes (food waste, grey and black water) with increased resource recovery through anaerobic digestion, ammonia stripping, struvite precipitation and pelletization. The Planetary Boundaries LCA (PB-LCA) results showed that both systems exceeded eight of the assigned shares of PBs, including climate change and biogeochemical flows of nutrients. Traditional LCA (ReCiPe impact assessment) showed net savings for the H+ system in a few categories and considerable reductions in several impacts, e.g., global warming potential (GWP), stratospheric ozone depletion, terrestrial acidification, and water consumption. In PB-LCA the H+ system gave additional impacts in both assessments for a few categories, mostly due to high consumption of chemicals in the ammonium stripping process used for nutrient recovery. In conclusion, the combined assessments highlight hot-spots for process optimization in the H+ system. From a methodological standpoint, PB-LCA still needs improvements to better reflect avoided burdens and results from traditional LCA should be fully transparent and analyzed carefully. The assessment methods complement each other and can be combined to better represent environmental performances of systems.

Model-based comparison of biological wastewater and sludge treatment combinations for nutrient removal, sludge and biogas production

One of the strategies for reducing the potential environmental and health-related risks associated with wastewater treatment sludge is to select treatment options that would minimize its production while using its beneficial products and meeting effluent discharge standards. In this regard, simulation models can aid in estimating treatment performance, sludge production, and biogas formation. This study evaluates effluent concentrations focusing on nutrients, sludge production, and biogas generation rates for various combinations of wastewater and sludge treatment alternatives for varying strengths of domestic wastewater. Overall, 105 scenarios were simulated for three different wastewater strengths for 35 treatment schemes. Bardenpho-5 (B5) was superior over A2O (A2) in removing nitrogen and phosphorous. Adding primary sedimentation to A2 and B5 models improved sludge and in turn biogas production. Effluent total phosphorus (TP) was usually higher in scenarios with anaerobic sludge digestion. However, struvite precipitation following anaerobic digestion helped reduce total nitrogen (TN) and TP effluent concentrations for all wastewater treatment technologies. Sludge production amounts ranged between 2865 kg/day to 38,709 kg/day (total solids) for an influent flow rate of 100,000 m3/day. Sludge production rates were relatively insensitive to sludge treatment alternatives for extended aeration (EA) compared to conventional activated sludge (AS), A2, A/O (AO), and B5. Generally, the impact of a sludge treatment option was significant for wastewater treatment technologies with higher waste sludge production. Thermal hydrolysis followed by anaerobic digestion provided the lowest sludge production and significantly improved the biogas production.

Aerobic degradation of parent triisobutyl phosphate and its metabolite diisobutyl phosphate in activated sludge: Degradation pathways and degrading bacteria

Although organophosphate esters (OPEs) degradation has been widely studied, the degradation of their metabolites is always ignored. Triisobutyl phosphate (TiBP), a typical alkyl-OPEs, is of emerging concern because of its potential ecotoxicity in the environment. This study provides comprehensive understanding about the degradation of TiBP and one of its metabolites, diisobutyl phosphate (DiBP) using activated sludge (AS). The results showed that TiBP and DiBP were degraded mainly through hydrolysis, dehydrogenation, and hydroxylation. The degradation kinetics indicated that DiBP had similar transformation rates to its parent TiBP in AS, highlighting the importance of metabolite DiBP study. Dehydrogenase, hydroxylase, phosphotriesterase, phosphodiesterase, and phosphomonoesterase played an important role in contributing to TiBP and its metabolites degradation via enzyme activity analysis. Besides, the expression of genes encoding these enzymes in bacteria and the relative abundance change of bacterial populations indicated that Sphingomonas and Pseudomonas may be the degrading bacteria of TiBP and Pseudomonas may be the main degrading bacteria of DiBP. This study provides new perspectives for metabolite DiBP and its parent TiBP degradation. It highlights that the formation and degradation of metabolites must be considered into the future researches.

Primary settling changes the microbial community of influent wastewater to wastewater treatment plants

The continuous immigration of bacteria in influent wastewater strongly impacts the microbial community of activated sludge (AS) in wastewater treatment plants (WWTP), both in terms of species composition and their abundance. Therefore, it is of interest to elucidate the route of immigrating bacteria into the biological tanks, including the effect of primary settlers. These are commonly used pretreatment units that can possibly selectively increase or reduce the relative abundance of certain bacteria. Species-level identification of the microbial composition of influent wastewater before and after primary settling was carried out in four full-scale municipal WWTPs biweekly over one year by 16S rRNA gene amplicon sequencing. Overall, 37–49% of incoming COD was removed in the primary settlers. Most genera and species were present in the wastewater to all four plants and the trend of these were investigated across the primary settlers. Approximately 50% of the genera had the same trend across at least three WWTPs. Few genera significantly increased in relative read abundance (3.7%) after settling, while 22.3% showed a significant reduction in relative abundance. We investigated process-critical species in AS, such as known nitrifiers, polyphosphate-accumulating organisms, and filamentous bacteria. Most taxa were affected similarly in all WWTPs including multiple genera involved in bulking in AS. However, some genera, e.g., important polyphosphate-accumulating bacteria, had inconsistent trends across WWTPs, suggesting that the characteristics of the wastewater are important for the trend of some bacteria through primary settling. In all cases, primary settling changed the microbial community of the influent wastewater, posing an obvious candidate for upstream control to optimize the assembly of the microbial communities in activated sludge.

Biodefoamer-Supported Activated Sludge System for the Treatment of Poultry Slaughterhouse Wastewater

Poultry slaughterhouse wastewater (PSW) is laden with fats, oil, and grease (FOG), as well as proteins. As such, PSW promotes the proliferation of filamentous organisms, which cause foam formation. In this study, the production of biological defoamers (biodefoamers) uses a consortium with antagonistic properties, i.e., 1.39 L of wastewater/mL defoamers, as reported in our previous study, toward foam formers and their application in the treatment of PSW using a bench-scale activated sludge (AS)-supported treatment system consisting of an aeration and clarification tank. The foam produced was slimy, brown, and thick, suggesting the presence of Nocardia, Microthrix, and Type 1863 species in the PSW/AS wastewater treatment system. The bio (Bio-AS) and synthetic-defoamers (Syn-AS, positive control) supplementation, i.e., at 4% v/v in the PSW/AS primary treatment stage (aeration tank) operated over ten days, resulted in 94% and 98% FOG and protein removal for the biodefoamers, respectively, when compared to 50% and 92% for a synthetic defoamer, respectively. Similarly, the Bio-AS treatment achieved 85.4% COD removal, while a lowly 51% was observed for the Syn-AS PSW treatment regime. Overall, the biodefoamers performed vehemently compared to synthetic defoamers, improving the PSW/AS system’s performance. It was prudent to hypothesize that the biodefoamers might have had FOG solubilization attributes, an assertion that needs further research in future studies. It was concluded that Bio-AS was more efficient in the removal of FOG, proteins, TSS, and COD in comparison to Syn-AS and negative control without supplementation (CAS).

The evaluation of aluminum and iron metal oxide settling behaviors for Hanford insoluble solids waste preprocessing

AbstractThe Hanford site is currently one of the largest and most expensive cleanup sites for hazardous, radioactive waste. Over 20% of the waste found at the Hanford site is in the form of a high activity sludge. The insoluble solids in the sludge will need to be concentrated prior to vitrification in the high‐level waste (HLW) melter. This will minimize the amount of liquid that will be evaporated during the melting process and expedite the melter processing rate. One proposed option for concentrating the insoluble solids is gravity settling in the storage tanks. Metal oxide compounds containing aluminum and iron make up the majority of the insoluble solids in the sludge, therefore understanding the behavior of these compounds in various tank waste matrices can facilitate sludge pretreatment options. A study of non‐radioactive slurry solutions containing Al(OH)3 (gibbsite), AlO(OH) (boehmite), and Fe2O3 (iron (III) oxide) was conducted to determine the time dependent interface behavior and settling rates of these compounds. Variations in solids loading and sodium concentration were evaluated to represent waste processing conditions and the results of these settling studies were compared with prior tank waste settling tests. Information gathered from these studies can be used to inform future decisions on sludge treatment processes of the insoluble solids processed at the Hanford site.

Activated sludge microbiome with H2O2-modified biochar enhances the treatment resilience and detoxification of oxytetracycline and its toxic byproducts

The widespread presence of oxytetracycline (OTC) in aquatic ecosystems poses both health risks and ecological concerns. The present study revealed the beneficial role of hydrogen peroxide (H2O2)-pretreated biochar (BC) derived from agricultural hardwood waste in an activated sludge (AS) bioprocess. The BC addition significantly enhanced the removal and detoxification of OTC and its byproducts. BC was initially modified using H2O2 to improve its OTC adsorption. Two AS reactors were then established, one with H2O2-modified BC and one without, and both were exposed to OTC. The BC-added reactor exhibited significantly higher OTC removal rates during both the start-up (0.97 d−1) and steady-state (0.98 d−1) phases than the reactor without BC (0.54 d−1 and 0.83 d−1, respectively). Two novel transformation pathways for OTC were proposed, with four byproducts originating from OTC identified, some of which were found to be more toxic than OTC itself. The BC-added reactor had significantly higher system functioning in terms of its heterotrophic activity and the reduction of the toxicity of OTC and its byproducts, as illustrated by structure-based toxicity simulations, antimicrobial susceptibility experiments, analytical chemistry, and bioinformatics analysis. Bioinformatics revealed two novel bacterial populations closely related to the known OTC-degrader Pandoraea. The ecophysiology and selective enrichment of these populations suggested their role in the enzymatic breakdown and detoxification of OTC (e.g., via demethylation and hydrogenation). Overall, the present study highlighted the beneficial role of H2O2-modified BC in combination with the AS microbiome in terms of enhancing treatment performance and resilience, reducing the toxicological disruption to biodiversity, and detoxifying micropollutants.

Tracing morphological characteristics of activated sludge flocs by using a digital microscope and their effects on sludge dewatering and settling

ABSTRACT In wastewater treatment by the activated sludge (AS) process, settleability and dewaterability of AS are key issues that are directly related to the treated water quality and sludge treatment costs. Several studies investigated the relationship between the shape of AS flocs and their settling/dewatering property. To quantify the floc morphology, it is imperative to attach a camera to a microscope or move the stage manually. Hence, labour and equipment costs may increase. In this study, by combining a digital microscope and an automatic stage, more than 100 magnified floc images were rapidly obtained from one AS sample dropped on a slide glass, and shape parameters were collectively calculated using an analysis software. During 1-year monitoring of four wastewater treatment plants in Sapporo City (Hokkaido, Japan), the morphological parameters and extracellular polymeric substances (EPS) quantity/quality of AS were analyzed on the basis of their correlation to the time to filtration (TTF) and sludge volume index (SVI), which are indicators for describing the dewatering and settling properties of AS, respectively. In one plant, larger, denser, and smoother flocs tended to contain less EPS and exhibited better sludge dewaterability. In another plant, larger, denser, and smoother flocs were considered to contribute to better settlement. Especially, an equivalent high-density floc diameter and the ratio of mixed liquor suspended solids (MLSS) concentration to the total floc area were commonly suggested to explain AS dewaterability and settleability.