The responsiveness of the POMC system to exogenous stimuli and the diurnal and seasonal rhythmicity of ACTH and β-endorphin (βE) in plasma were studied in outdoor-reared domestic ganders. Plasma levels of ACTH- and βE-like immunoreactivities were determined by direct and specific radioimmunoassays. In the first series of experiments immunoreactive (ir) ACTH and βE were measured in the plasma of male domestic geese after 5 min of ether stress and after administration of 2 μg/kg lipopolysaccharide (LPS). Both ir-ACTH and ir-βE levels increased 5 and 10 min after ether inhalation, but the increase in the ir-βE concentration was only half that of the ir-ACTH. The plasma ir-ACTH levels were elevated after 60 and 120 min but not after 90 min of LPS administration; ir-βE levels were unchanged at all time points. In a second series of experiments blood samples were taken on 30 March, 16 June, 4 August, and 27 October. On these days diurnal samplings were performed at 02:00, 06:00, 10:00, 14:00, 18:00, and 22:00 h. A two-way analysis of variance showed significant diurnal and seasonal changes for both hormones and significant interaction between the diurnal and seasonal levels. The highest daily mean values of the plasma ir-ACTH and ir-βE concentrations were measured in June. The maximum of the ir-ACTH level was at 10:00 h in March and August, but at 22:00 h in June and October. The changes in ir-βE concentrations paralleled those of ir-ACTH, but the changes did not reach statistical significance in every case.