ABSTRACT We compared the effects of four seeding rates, 60, 100, 140, and 180 seeds m−2, on yield, lodging resistance and culm strength using two Japanese wheat cultivars Ayahikari and Iwainodaichi. With the decreasing seeding rate, maximum tiller number decreased, percentage of productive culms increased and spike number m−2 decreased, and the spikelet number per spike, percentage of fertile spikelets, grain number per spikelet and grain number per spike increased. Consequently, grain yield was not significantly affected. Lodging was not observed, but the lodging index was significantly affected by the seeding rate and was the highest in the plots sown at 180 seeds m−2. The bending moment at the breaking point and flexural rigidity of the basal internode with leaf sheaths were highest in the plots sown at 60 seeds m−2. In conclusion, the culm strength and stem lodging resistance increased with decreasing seeding rate. Section modulus and bending stress, which constitute the bending moment at the breaking point, and the moment of inertia area and Young’s modulus, which represent the flexural rigidity, were highest in plots sown at 60 seeds m−2. Therefore, the seeding rate was considered to influence the culm morphology and functions of the cell wall components. The effects of the seeding rate on culm characteristics and lodging resistance did not differ between the two cultivars, and ‘Ayahikari’ had consistently stronger culms than ‘Iwainodaichi’. We suggest that low seeding rates method would be suitable for heavily fertilized cultivation for both cultivar with high and low culm strength. Abbreviations AMeDAS: the automated meteorological data acquisition system; HSD: honestly significant difference