Objective To investigate the effect of glutamine(GLN) fortifying enteral nutrition (EN) intervention on nutrition status and lymphocytes-mediated immune function in children with acquired immune deficiency syndrome(AIDS). Methods According to random number table, the 58 cases of AIDS patients conformed to the standard were divided into observation group (GLN improved group, 30 cases) and control group (conventional EN, 28 cases). Nutrition related index, index of cellular immunity and serum GLN concentration change of the 2 groups were detected before intervention and after intervention for 28 days. Results Body mass in the observation group after intervention for 28 days[(29.9±7.4) kg vs(28.0±8.3) kg, t=3.216, P=0.006], body mass index [(17.8±1.9) kg/m2vs(16.9±1.9) kg/m2, t=3.339, P=0.002], total lymphocyte count [(2.81±0.25)×109/L vs(2.53±0.24)×109/L, t=3.064, P=0.004], serum level of GLN[(7.2±2.1) μmol/L vs(5.5±1.2) μmol/L, t=2.496, P=0.012] were notably increased compared with those of control group.In observation group albumin [(40.5±3.1) g/L vs(38.6±2.1) g/L, t=3.82, P=0.001], pre-albumin [(182.0±38.6) mg/L vs(175.1±40.0) mg/L, t=2.917, P=0.007], hemoglobin [(115.7±8.5) g/L vs (104.8±11.5) g/L, t=3.022, P=0.005], CD4+ %(39.0±7.5)% vs (35.3±9.9)%, t=3.138, P=0.004], CD4+ /CD8+ ratio[(1.6±0.8) vs (1.2±0.6), t=3.198, P=0.001]were significantly higher than those of the control group.Incidence rate of gastrointestinal complications of the observation group were lower than that of the control group(16.7% vs 32.1%, χ2 =11.25, P=0.001). Conclusions Enhanced GLN EN intervention can improve nutritional status in AIDS children, raise the level of lymphocyte immunity, reduce gastrointestinal complications and new infections, shorten hospitalization time, and improve the prognosis of children with AIDS, So it is worthy of clinical promotion and application. Key words: Glutamine; Enteral nutrition; Acquired immune deficiency syndrome; Nutrition and immunity