The results of formation of broodstocks and commercial productive stocks of sterlet (Acipenser ruthenus Linnaeus, 1758), beluga (Huso huso Linnaeus, 1758) and bester (Huso huso × Acipenser ruthenus) on the Samukh commercial farm in Republic of Azerbaijan are presented. In 2019, for the first time in the history of fishbreeding in Azerbaijan, a progeny was received from grown “from eggs” sterlet females at age of 4 years at the Samukh fish farm using RAS. The mature eggs were stripped using minimally invasive microsurgery method by S.B. Podushka. After the egg collection sterlet females were transferred into holding basins for rehabilitation, further growing and second maturation. The total number of sterlet larvae from domestic broodstock exceeded 100 thousand. An ovulated beluga eggs were received from grown in aquaculture females at the age of 15 in December 2020. In result of fertilization of beluga eggs by sterlet sperm, hybrid (♀ Huso huso × ♂ Acipenser ruthenus) progeny was obtained and successfully grown on the farm. In future the domestic bester broodstock would be formed. The received data demonstrated that functional development of fingerlings and juveniles of sterlet “from eggs” sterlet farm took place without noticeable deviations from physiological normal physiological indices. The number of red blood cells per unit volume of blood increased in ontogenesis. On the contrary the number of white blood cells had seasonal variability and did not directly depend on the age of young sterlet.