Abstract
In the present study, the biological function of recombinant gonadotropin-releasing hormone associate peptide (rGnRH) was tested for the induction of ovulation and spawning in Sterlet sturgeon (Acipenser ruthenus) broodstock. For this purpose, Sterlet sturgeon broodstock were divided into four treatments and treated with following preparing procedure: 0.9% NaCl (C); 2.5 μg kg body weight−1 (BW) LHRH-A2 (luteinizing hormone-releasing hormone-A2, L2.5); 2.5 μg kg BW−1 rGnRH (rGn2.5); 10 μg kg BW−1 rGnRH (rGn10). The capability of the rGnRH for eliciting biological response was studied in vivo by evaluating the changes of 17β estradiol (E2), progesterone (P) and testosterone (T) and by its ability for inducing final maturation. Blood samples were collected at the time of first injection, 10 h after the first injection and at ovulation time. The L2.5 or rGn10 treatments led to significantly lower E2 concentration 10 h after the first injection and at ovulation time compared to the baseline measures. E2 levels increased in the rGn2.5 treatment 10 h after the first injection and by ovulation time compared to the time of first injection. T levels were significantly increased in the L2.5 or rGn10 treatments 10 h after the first injection compared to the time of first injection and it further decreased at ovulation time. No significant differences were found in C and rGn2.5 treatments between the different sampling times. P secretion was significantly higher in the rGn10 or L2.5 treatments compared to C and rGn2.5 treatments 10 h after the first injection and at ovulation time. No significant difference was found in P secretion in the C and rGn2.5 treatments at different sampling times. The results showed that the rGnRH at the highest dose (10 μg kg BW−1) was more efficient than the other treatments in the induction of spawning and this dose caused P secretion and spawning in vivo. Our results present the possibility of using rGnRH in artificial reproduction of sturgeons and it can be introduced as a specific homologue to treat reproductive disorders in fish.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
