Acid Buffer Research Articles

Paintable, Fast Gelation, Highly Adhesive Hydrogels for High-fidelity Electrophysiological Monitoring Wirelessly.

High-fidelity wireless electrophysiological monitoring is essential for ambulatory healthcare applications. Soft solid-like hydrogels have received significant attention as epidermal electrodes because of their tissue-like mechanical properties and high biocompatibility. However, it is challenging to develop a hydrogel electrode that provides robust contact and high adhesiveness with glabrous skin and hairy scalp for high-fidelity, continuous electrophysiological signal detection. Here, a paintable, fast gelation, highly adhesive, and conductive hydrogel is engineered for high-fidelity wireless electrophysiological monitoring. The hydrogel, consisting of gelatin, gallic acid, sodium citrate, lithium chloride, glycerol, and Tris-HCl buffer solution exhibits a reversible thermal phase transition capability, which endows it with the attributes of on-skin applicability and fast in situ gelation with 15 s, thereby addressing the aforementioned limitations. The introduction of gallic acid enhances the adhesive properties of the hydrogel, facilitating secure electrode attachment to the skin or hairy scalp. To accentuate the potential applications in at-home electrophysiological health monitoring, the hydrogel electrodes are demonstrated for high-fidelity electrocardiogram recording for one hour during various daily activities, as well as in simultaneous electroencephalogram and electrocardiogram recording during a 30 min nap.

PH Determination of Acetic Acid-Sodium Acetate Buffer: An Application of Henderson-Hasselbalch Equation at Room Temperature

In article, we have reported the applying of Henderson-Hasselbalch equation in determination of pH of acetic acid and sodium acetate buffer solutions at about 298 Kelvin as room temperature. In preparation of these buffer solutions, we have taken 1.5 ml of glacial acetic acid into 100 ml of standard flask for making up 0.2M of acetic acid solution, and 0.64 gm of sodium acetate were dissolved into 100 ml distilled water. Now, we taken 36.2 ml prepared solution of sodium acetate into 100 ml of standard flask and added 14.8 ml of prepared acetic acid to make its volume 100 ml by using of distilled water, it is a buffer. In measuring of pH of prepared buffer solutions, we have been using a calibrated digital pen pH meter for accurate and quick reading. In observation, the pH of buffer solutions a quite fluctuation was appeared. But, it has gradually raised by adding small amount of basic 5N NaOH solution into buffer sample, and, an adjusted pH was being to 4.7 as well.

Bioactive Self-Polymerizing Resin with Surface Pre-Reacted Glass Ionomer Fillers for Suppressed Enamel Demineralization.

The treatment of damaged enamel surfaces involves modification of the enamel surface with artificial materials or the development of a pseudo-enamel, with research focusing on bioactive and biomimetic materials. In this study, a bioactive auto-polymerizing resin (APR) was developed by adding surface-pre-reacted glass ionomer (S-PRG) fillers of different quantities to APR. Its bioactive effects were evaluated via pH neutralization, ion release, and inhibition of enamel demineralization studies. The pH and fluoride ion release were measured using ion-specific electrodes, revealing that the APR disk with the S-PRG filler immediately neutralized the lactic acid solution (pH 4.0) through ion release. Inductively coupled plasma atomic emission spectrometry revealed that the Sr ion release peaked on the first day, with the other ions following the order F > B > Si > Al > Na, exhibiting a weekly decrease in the same order. Scanning electron microscopy was used to examine the enamel block morphology of the disks after 7 d of incubation, revealing enamel demineralization in disks without the S-PRG filler, whereas no demineralization occurred in disks with the S-PRG filler. APR containing the S-PRG filler demonstrated acid buffering suppressed enamel demineralization and bioactive properties.

Examination of the impact of using lactose or permeate for protein standardisation of skimmed milk on viscosity characteristics during evaporation

The effect of permeate and lactose for protein standardisation of skim milk concentrate, was investigated during evaporation. Analysis of the viscosity–total solids (TS) profiles of each treatment demonstrated that the unstandardised protein sample had the greatest increase in viscosity, followed by permeate and finally lactose‐standardised samples. Heat stability and acid buffering capacity of the permeate standardised skim milk samples were higher when compared to the lactose‐standardised samples. This work identifies how standardisation media can impact the viscosity of concentrated skim milk, enabling higher TS at the evaporator outlet in some circumstances which could have the potential to improve overall process efficiency. Furthermore this work demonstrates that, evaporating to a target viscosity rather than target TS would offer greater control and consistency during processing.

Modified mesoporous silica nanocarriers containing superparamagnetic iron oxide nanoparticle, 5-fluorouracil or oxaliplatin, and metformin as a radiosensitizer, significantly impact colorectal cancer radiation therapy

This study investigates the anticancer effects of SPION-based silica nanocarriers carrying 5-fluorouracil (5-FU) or oxaliplatin (OX), and metformin (MET) on colorectal cancer cells. Nanocarriers were equipped with pH-responsive gold gatekeepers for controlled release, PEGylation for longer circulation, and folic acid (FA) for targeted delivery. The effects were evaluated by investigating cell viability, cellular uptake, flow cytometry, and clonogenic assay in vitro. The efficacy of the system was also tested in vivo on C57BL/6 mice bearing HT-29 tumors, and potential side effects were evaluatedNanocarriers were synthesized with hydrodynamic diameters of 79.8 nm for 5-FU and 85.2 nm for OX; zeta potentials of −21 and –22 mV, respectively, and remained stable after 72 h. Encapsulation efficiencies were 85 % for 5-FU, 80 % for OX, and 83 % for MET, with loading capacities of 44 %, 38 %, and 41 %, respectively. Drug release in acidic buffer was 38.7 % for 5-FU, 32.8 % for OX, and 43.5 % for MET. MTT assay showed increased toxicity due to FA conjugation, while PEGylation reduced the hemolysis activity. Targeted nanocarriers demonstrated superior cellular uptake and tumor localization compared to non-targeted variants. The combination of 5-FU-MET and OX-MET nanocarriers with radiation therapy (RT) demonstrated the greatest effect on their antitumor activity, accompanied by minimal side effects indicating effective tumor targeting in vivo. MRI and CT imaging further supported these findings. This study underscores the synergistic impact of MET alongside RT on the inhibition of cancer cells and tumor growth for both targeted 5-FU and OX nanocarriers reflecting the significant radiosensitizing properties of MET.

Applicability of QbD-assisted Analytical Method for Simultaneous Detection of Tetrahydrocurcumin and Folic Acid in Developed Nanostructured Lipid Carriers

Aims: Applicability of QbD-assisted analytical method for simultaneous detection of tetrahydrocurcumin and folic acid in developed nanostructured. Background: Diabetic foot ulcer (DFU) is a multifactorial disorder that involves chronic inflammation, oxidative stress and neuropathy. Current treatment therapies involving the use of growth factors and skin substitutes being costly, are out of reach for the majority of patients. The present research explored the usefulness of (5929IN008, application number 202211045937), a combination of tetrahydrocurcumin and folic acid-loaded nanostructured lipidic carriers. Objectives: To develop and validate a QbD-assisted method for simultaneous analysis of tetrahydrocurcumin (THC) and folic acid (FA). Applicability of the above method to determine total drug content (TDC) and entrapment efficiency (EE) of nanostructured lipid carriers (NLCs) loaded THC and FA. Methods: A high-performance liquid chromatographic (HPLC) method was developed, optimized and validated using Box-Behnken design for improved method performance. Chromatographic separation was conducted on a Supelco 250 x 4.6 mm (5 μm) column with optimized mobile phase composition containing tetrahydrofuran: citric acid buffer pH 3.5 (50:50) at a flow rate of 0.4 mL.min-1 and diode array detection between 210 and 360 nm. Results: The method developed in a concentration range of 1 to 100 μg.mL-1 was found to be linear (R2 0.999, p≤0.001), accurate (99.10-101.70%) and precise with high recovery values in intra and inter-day results. The system adaptability and robustness evaluation revealed that the percent recovery ranged from 96.90 to 102.80%, and the percent relative standard deviation (%RSD) values were less than 2%. Moreover, the method was further applied for the determination of TDC (86±6% and 96±8%) and drug EE (81±21% and 73±13%) for THC and FA, respectively. Conclusion: The investigation indicated the applicability of the developed and validated method for the estimation of THC and FA in the developed nanostructured lipidic carriers.

Modified streptozotocin-induced diabetic model in rodents.

Streptozotocin (STZ)-induced type I diabetes mellitus (DM) models have been pivotal in diabetes research due to their ability to mimic the insulin-dependent hyperglycemia akin to human type I diabetes. However, these models often suffer from poor induction rates and low survival post-STZ induction, especially in long-term experiments, necessitating insulin supplementation, which introduces additional variables to experiments. To address this, we present a novel modification to the STZ-induced DM model in C57BL/6J mice to improve survival rates without insulin supplementation. Our method involves non-fasting, low-dose STZ injections dissolved in pH-neutral phosphate buffer saline instead of acidic sodium citrate buffer, administered over 5 days. We observed hyperglycemia induction in 94.28% of mice within a week post-injection, with stable high blood glucose levels, stable body weight, and minimal mortality up to 21 weeks. Notably, omitting 10% sucrose in water and fasting did not affect hyperglycemia induction. Our findings suggest that the modified protocol not only decreases the experimental effort of the researchers, but reduces animal stress and mortality, thus enhancing experimental outcomes and animal welfare. By optimizing the STZ-induced DM model in C57BL/6J mice, our study provides a valuable resource for researchers aiming to study diabetes and its complications while minimizing experimental variability and animal usage.

A combination of carbonates and Opuntia ficus-indica extract protects esophageal cells against simulated acidic and non-acidic reflux in vitro

Buffering of stomach acid by antacids is a well-established symptomatic therapy for heartburn. In addition, preparations from prickly pear (Opuntia ficus-indica) have been shown to reduce tissue damage in experimental gastritis models and to attenuate gastrointestinal discomfort in patients. Both active principles have been included in a fixed-combination product for symptomatic treatment of heartburn containing carbonate antacids (CaCO3 and MgCO3) and an extract from Opuntia ficus-indica cladodes. The aim of the study was to characterize the acid neutralization and esophageal cell protective activities of the product and its individual active ingredients in a set of in vitro assays. Acid neutralization was assessed in a simulated stomach model. Protective activity of individual constituents and in combination was analyzed in an esophageal cell line (COLO-680 N) exposed to low pH and deoxycholic acid to simulate acidic and non-acidic reflux challenge. The combination product protected cells against low pH mediated cytotoxicity via acid neutralization by carbonates. Opuntia extract itself and the combination product attenuated bile acid-induced cell irritation as measured by reduced release of proinflammatory interleukin-6 and -8. In conclusion, addition of Opuntia extract to a mineral antacid provides dual protection against acidic and non-acidic simulated reflux challenge.

Chemoselective oxidative N-debenzylation of aryl halogenated amines using the Laccase LAC97/TEMPO system

The N-debenzylation of aryl-halogenated amines using the Laccase LAC97/TEMPO system is reported, demonstrating selective N-debenzylation with no dehalogenation of the phenyl ring, which is observed with conventional debenzylation methods such as palladium-catalysed hydrogenation. This reaction was performed under ambient conditions in acidic buffer with 5 % DMSO as co-solvent in an open-to-air vessel. The versatility and robustness of the system was demonstrated on substrates harbouring different halogen moieties. The limitations of the Laccase LAC97/TEMPO system were also studied, highlighting the formation of by-products due to overoxidation on selected substrates. A series of substrates with various halogens were studied. High conversion (78–90 %) in 6 h and full conversion was achieved within 24 h using HPLC to monitor the reaction. Overall, this system is presented as a chemoselective N-debenzylation alternative for aryl-halogenated substrates.

Investigation of the Application of Reduced Graphene Oxide-SPION Quantum Dots for Magnetic Hyperthermia.

Integrating hyperthermia with conventional cancer therapies shows promise in improving treatment efficacy while mitigating their side effects. Nanotechnology-based hyperthermia, particularly using superparamagnetic iron oxide nanoparticles (SPIONs), offers a simplified solution for cancer treatment. In this study, we developed composites of SPION quantum dots (Fe3O4) with reduced graphene oxide (Fe3O4/RGO) using the coprecipitation method and investigated their potential application in magnetic hyperthermia. The size of Fe3O4 nanoparticles was controlled within the quantum dot range (≤10 nm) by varying the synthesis parameters, including reaction time as well as the concentration of ammonia and graphene oxide, where their biocompatibility was further improved with the inclusion of polyethylene glycol (PEG). These nanocomposites exhibited low cytotoxic effects on healthy cells (CHO-K1) over an incubation period of 24 h, though the inclusion of PEG enhanced their biocompatibility for longer incubation periods over 48 h. The Fe3O4/RGO composites dispersed in acidic pH buffer (pH 4.66) exhibited considerable heating effects, with the solution temperature increasing by ~10 °C within 5 min of exposure to pulsed magnetic fields, as compared to their dispersions in phosphate buffer and aqueous dimethylsulfoxide solutions. These results demonstrated the feasibility of using quantum dot Fe3O4/RGO composites for magnetic hyperthermia-based therapy to treat cancer, with further studies required to systematically optimize their magnetic properties and evaluate their efficacy for in vitro and in vivo applications.

Novel Electrospun Zwitterionic Nanofibers for Point‐Of‐Care Nucleic Acid Isolation Strategies Under Mild Conditions

AbstractNucleic acid (NA) testing at the point‐of‐care requires efficient NA extraction followed by post‐NA amplification to achieve necessary detection sensitivity. Nanofibers (NFs) are demonstrated to be an ideal solid surface in an NA extraction process but necessitate harsh conditions that interfere with the subsequent NA amplification process. It is demonstrated that novel, pH tunable, zwitterionic NFs composed of uncharged nylon doped with the weakly basic, cationic polyallylamine hydrochloride and the weakly acidic anionic polycarboxylic acid to address the issue. Unlike the other cationic polymers investigated, e.g. polybrene and polyaniline, these polymers allow efficient NA extraction in Tris‐ethylenediamine tetra‐acetic acid buffer under mild conditions (pH 4.5 containing 0.1% Tween 20 for adsorption, and pH 10 with 50 mM NaCl for elution). Adsorption and elution yields over 95% and 70%, respectively, are achieved. It also discovered a correlation between material morphologies and the NA extraction suggests that the combination of polymer chemistries and nanofiber morphologies facilitates efficient NA extraction at low concentrations (ng range) within a short time period (<10 min). Considering the simple protocols and instrument‐free operation the as‐developed NFs are highly attractive for use in sample‐to‐answer NA testing in point‐of‐care settings.

Polysorbate stability: Effects of packaging materials, buffers, counterions, and pH

Polysorbates, widely used excipients in drug formulations, present a stability challenge due to complex degradation processes. This study investigates the hydrolysis of polysorbate (PS) under temperature stress (50 °C), focusing on the impact of primary packaging materials (glass vs. plastic vials), buffers (histidine and acetic acid), counterions (chloride vs. malate), and pH (4–7). Our findings reveal that leachables from plastic vials inhibit PS degradation in both histidine and acetic acid buffers. Kinetic parameters derived from sigmoidal fitting suggest distinct degradation mechanisms for each buffer. Furthermore, the malate counterion with histidine displays inhibitory effects on PS hydrolysis. Principal component analysis was employed to identify key factors. These results highlight the critical role of excipients and packaging in PS stability, providing valuable insights for biopharmaceutical formulation development and a deeper understanding of PS degradation complexities.

Deep insights into the enhanced organic decontamination by Cu(II)/peracetic acid system in boric acid buffer: Experimental and computational evidences of the crucial role of H2O2

Boric acid (BA) has been frequently utilized as a buffer in advanced oxidation processes (AOPs) under weakly alkaline conditions. This study unveiled a notable enhancement in the treatment of rhodamine B (RhB) achieved with the Cu(II)/PAA system operated in BA solution. Upon exploring the reaction mechanisms of the BA/Cu(II)/PAA system, it was intriguingly discovered that the formation of bidentate BA-Cu(II) complex (CuH2BO3+) could effectively boost the reactivity of Cu(II) with H2O2. This improvement promoted the generation of Cu(I) and subsequent PAA activation and RhB degradation. Conversely, the tridentate BA-Cu(II) complex (Cu(H2BO3)2(aq)) exhibited a diminishing effect. Furthermore, differential charge density calculations revealed distinct electron transfer dynamics between H2O2 and CH3C(O)OOH complexes with CuH2BO3+, highlighting H2O2 as the preferential electron-donating compound in BA/Cu(II)/PAA system. Results indicated that CH3C(O)O• was the major contributing radical, followed by 1O2, to RhB degradation. Overall, this investigation provides valuable insights into the synergistic utilization of BA and copper as a novel strategy for enhancing the performance of copper-based AOPs on treating water contaminants. Additionally, it sheds light on the optimal dosage of BA when employing BA as the buffer.

The Effect of Assessment for Learning in Chemistry Learning on Students' Learning Outcomes

This research aims to describe the effect of the implementation of assessment for learning (AfL) in chemistry learning on student learning outcomes. This type of research is pre-experiment. The design used in this research is One Group Pretest Posttest Design, with the same treatment in class XI, that is pretest - meeting 1 - posttest 1 - meeting 2 - posttest 2. Meetings 1 and 2 respectively are the implementation of AfL on the material of acidic buffer solutions and basic buffer solutions. The subjects of this research were students of class A and B SMAN 2 Bangkalan. Students' chemistry learning outcomes were obtained from the results of pretest, posttest 1, and posttest 2. Data analysis techniques using normality test followed by hypothesis testing and N-Gain test. The results showed that there was a significant difference between the pretest and posttest 1 and between posttest 1 and posttest 2 in classes A and B. Students' learning outcomes also improved based on the results of the N-Gain test. Based on the results of the research, it can be concluded that the implementation of AfL in learning chemistry has an effect on learning outcomes and is able to improve student learning outcomes

A Quality by Design (QBD) Approach for the Development and Validation of RP-HPLC Method for the Quantification of Silymarin Tablet

This study emphasizes the crucial role of Quality by Design (QbD) in developing pharmaceutical procedures, particularly in risk assessment. It demonstrates how QbD principles were applied to create a precise and effective HPLC method for Silymarin Tablets, ensuring consistent quality within specified criteria. The optimized method, developed using a Design of Experiment approach, employs a C18 column (150 mm x 4.6 mm, 5μm) with isocratic elution using a 95:25 ratio of acetonitrile to orthophosphoric acid buffer (pH 3). Peaks were detected using a PDA detector calibrated at 287 nm, with a flow rate of 1.0 mL/min. The column oven temperature was maintained at 25°C, and a 10 μL injection volume was used. Thorough validation, adhering to USP <1225> and ICH Q2 (R1) standards, ensures the method's reliability. Key factors such as accuracy, precision, robustness, limit of detection (LOD), and limit of quantification (LOQ) were comprehensively assessed. The method exhibits exceptional sensitivity, selectivity, efficiency, precision, accuracy, and cost-effectiveness, making it ideal for pharmaceutical analysis of Silymarin tablets. It has been validated to effectively differentiate between marketed products, including those closely resembling the original. This method is intended for routine quality control analysis in the pharmaceutical industry, highlighting its suitability and reliability for ongoing use.

Ionized copolyesters with pH-responsive degradability: Accelerated degradation in specific environments

The development of environmentally responsive biodegradable polymers is a promising solution for balancing the stability and degradability of biodegradable plastics. In this study, a commercial biodegradable polyester, poly(butylene adipate-co-butylene terephthalate) (PBAT), was used as the substrate and was synthetically modified with a small amount of anionic sodium 1–3-isophthalate-5-sulfonate (SIPA) to obtain the ionized random poly(butylene adipate-co-butylene terephthalate-co-butylene 5-sodiosulfoisophthalate) (PBATS). The introduction of the sodium sulfonate ionic group enhanced the mechanical and heat-resistant properties of the material, while significantly improving the hydrophilicity and water absorption of the copolyesters of PBATSs and endowing them with special pH-responsive degradation properties. Compared with PBAT, PBATS copolyesters could accelerate degradation in acidic or alkaline buffer solutions and natural seawater, while degradation was inhibited in neutral buffer solutions at pH 7.2. Degradation experiments in simulated gastric, intestinal, and body fluids revealed that the copolyester showed specific and rapid degradation in acidic gastric fluids. This environmentally-responsive degradable material greatly expands the special applications of biodegradable polyesters in the fields of environmental remediation and medical applications.

A simple and efficient approach for preparing cationic coating with tunable electroosmotic flow for capillary zone electrophoresis-mass spectrometry-based top-down proteomics

BackgroundCapillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has become a valuable analytical technique in top-down proteomics (TDP). CZE-MS/MS-based TDP typically employs separation capillaries with neutral coatings (i.e., linear polyacrylamide, LPA). However, issues related to separation resolution and reproducibility remain with the LPA-coated capillaries due to the unavoidable non-specific protein adsorption onto the capillary wall. Cationic coatings can be critical alternatives to LPA coating for CZE-MS/MS-based TDP due to the electrostatic repulsion between the positively charged capillary inner wall and proteoform molecules in the acidic separation buffer. Unfortunately, there are only very few studies using cationic coating-based CZE-MS/MS for TDP studies. ResultsIn this work, we aimed to develop a simple and efficient approach for preparing separation capillaries with a cationic coating, i.e., poly (acrylamide-co-(3-acrylamidopropyl) trimethylammonium chloride [PAMAPTAC]) for CZE-MS/MS-based TDP. The PAMAPTAC coating-based CZE-MS produced significantly better separation resolution of proteoforms compared to the traditionally used LPA-coated approach. It achieved reproducible separation and measurement of a simple proteoform mixture and a complex proteome sample (i.e., a yeast cell lysate) regarding migration time, proteoform intensity, and the number of proteoform identifications. The PAMAPTAC coating-based CZE-MS enabled the detection of large proteoforms (≥30 kDa) from the yeast cell lysate reproducibly without any size-based prefractionation. Interestingly, the mobility of proteoforms using the PAMAPTAC coating can be predicted accurately using a simple semi-empirical model. SignificanceThe results render the PAMAPTAC coating as a valuable alternative to the LPA coating to advance CZE-MS-based TDP towards high-resolution separation and highly reproducible measurement of proteoforms in complex samples.

Optimized inhaled LNP formulation for enhanced treatment of idiopathic pulmonary fibrosis via mRNA-mediated antibody therapy

Lipid nanoparticle-assisted mRNA inhalation therapy necessitates addressing challenges such as resistance to shear force damage, mucus penetration, cellular internalization, rapid lysosomal escape, and target protein expression. Here, we introduce the innovative “LOOP” platform with a four-step workflow to develop inhaled lipid nanoparticles specifically for pulmonary mRNA delivery. iLNP-HP08LOOP featuring a high helper lipid ratio, acidic dialysis buffer, and excipient-assisted nebulization buffer, demonstrates exceptional stability and enhanced mRNA expression in the lungs. By incorporating mRNA encoding IL-11 single chain fragment variable (scFv), scFv@iLNP-HP08LOOP effectively delivers and secretes IL-11 scFv to the lungs of male mice, significantly inhibiting fibrosis. This formulation surpasses both inhaled and intravenously injected IL-11 scFv in inhibiting fibroblast activation and extracellular matrix deposition. The HP08LOOP system is also compatible with commercially available ALC0315 LNPs. Thus, the “LOOP” method presents a powerful platform for developing inhaled mRNA nanotherapeutics with potential for treating various respiratory diseases, including idiopathic pulmonary fibrosis.

Hydrogen Absorption and Self-Corrosion of Mg Anode: Influence of Aqueous Electrolyte Species

This review examines the impact of various aqueous electrolytes on hydrogen absorption and self-corrosion in magnesium (Mg) anodes. The discussion integrates both historical and recent studies to explore the mechanisms behind self-corrosion and anomalous hydrogen evolution (HE) under conditions of the Negative Difference Effect (NDE) and Positive Difference Effect (PDE). The focus is on the formation and oxidation of magnesium hydride in regions of active dissolution under NDE conditions. In the case of PDE, anodic dissolution occurs through the passive MgO-Mg(OH)₂ film, which shields the metal from aqueous electrolytes, thereby reducing hydrogen absorption and abnormal HE. The NDE conditions showed delayed reduction activity at the surface, attributed to a hydride phase within the corrosion product layer. Hydride ions were quantified through their anodic oxidation in an alkaline electrolyte, measured by the electric charge passed. The review also considers the role of de-passivating halide ions, electrolyte acidity buffering, and the addition of ligands that form stable complexes with Mg2⁺ ions, on the rates of hydride formation, self-corrosion, and anodic dissolution of Mg. The study evaluates species that either inhibit or promote hydrogen absorption and self-corrosion.

Soil Health Intensification through Strengthening Soil Structure Improves Soil Carbon Sequestration

Intensifying soil health means managing soils to enable sustainable crop production and improved environmental impact. This paper discusses soil health intensification by reviewing studies on the relationship between soil structure, soil organic matter (SOM), and ecosystem carbon budget. SOM is strongly involved in the development of soil structure, nutrient and water supply power, and acid buffering power, and is the most fundamental parameter for testing soil health. At the same time, SOM can be both a source and a sink for atmospheric carbon. A comparison of the ratio of soil organic carbon to clay content (SOC/Clay) is used as an indicator of soil structure status for soil health, and it has shown significantly lower values in cropland than in grassland and forest soils. This clearly shows that depletion of SOM leads to degradation of soil structure status. On the other hand, improving soil structure can lead to increasing soil carbon sequestration. Promoting soil carbon sequestration means making the net ecosystem carbon balance (NECB) positive. Furthermore, to mitigate climate change, it is necessary to aim for carbon sequestration that can improve the net greenhouse gas balance (NGB) by serving as a sink for greenhouse gases (GHG). The results of a manure application test in four managed grasslands on Andosols in Japan showed that it was necessary to apply more than 2.5 tC ha−1 y−1 of manure to avoid reduction and loss of SOC in the field. Furthermore, in order to offset the increase in GHG emissions due to N2O emissions from increased manure nitrogen input, it was necessary to apply more than 3.5 tC ha−1y−1 of manure. To intensify soil health, it is increasingly important to consider soil management with organic fertilizers that reduce chemical fertilizers without reducing yields.