Acid Sodium Salt Research Articles

Effects of the diet enrichment with β-glucan, sodium salt of butyric acid and vitamins on growth parameters and the profile of the gut microbiome of juvenile African catfish (Clarias gariepinus)

This study assessed the effect of the addition of active substances, i.e., β-glucan, sodium butyrate and vitamins (C, A, D3, E, K) to the diet of juvenile African catfish (Clarias gariepinus) on growth parameters and the intestinal microbiome. For this purpose, a four-week rearing of juveniles African catfish in a recirculating aquaculture system (RAS) under controlled conditions at 25°C was conducted. The fish were fed commercial feed (control group - C), which was enriched with various levels of sodium butyrate and β-glucan (FQV1 – FQV4). After the experiment, growth parameters such as weight gain (WG; g), length gain (LG; cm), specific growth rate (SGR; % d−1), condition factor (K) survival rate (SR; %) and cannibalism factor (CF; %) were determined. The profile of the intestinal microbiome was also determined using Next-Generation Sequencing (NGS) based on the analysis of the 16 S rRNA subunit. The obtained results indicated that feed supplementation with active substances in appropriate doses had a positive effect on the growth parameters of tested fish. Compared to the control group, statistically significant differences were obtained in WG (C - 7.62 g; FQV3 - FQV1 respectively 12.2–16.65 g), in WL (C - 6.23 cm; FQV3 - FQV1 respectively 7.59–8.74 cm), in SGR (C - 4.7%/d; FQV3 – V1 respectively 12.20 – 16.65 g), in K (C – 0.76; V3 – FQV4 respectively 0.80 – 0.84) (P <0.05). In addition, the control group had the highest value of CF (8.0%) compared to the research groups (P <0.05). The values of α-diversity metrics did not show statistically significant differences. However, the number of unique amplicon sequence variants (ASVs) in research groups confirmed that enriching the diet with the active substances increases the biodiversity of intestinal microbiome (C - 203; FQV4 - 373). In research groups and the control group, Firmicutes and Proteobacteria dominated, additionally, an increased number of beneficial lactic acid bacteria (LAB) of the Lactococcus and Bacillus genus was observed, while the number of potentially pathogenic bacteria of the Candidatus genus was reduced (P <0.05). Principal coordinate analysis (PCoA) determining β-diversity showed that the FQV3 was characterized by varied species composition compared to the other groups. The obtained results showed that a carefully selected composition of active ingredients used in the diet of juvenile African catfish has a beneficial effect on the profile and biodiversity of the intestinal microbiome, which indirectly improves resistance to infections and increases breeding indicators.

Assessment of the Relationship Between Humic Acid Contents and Trace Elements of Some Agricultural Soils in Diyarbakır Region by Multivariate Statistical Methods

There are important relationships between humic acid (HA) and the bioavailability, reactions and mobility of trace elements in the soil. For this reason, soils are tried to be improved chemically, biologically and physically with HA applications. In this study, the relationship of humic acid contents of 118 agricultural soil samples from Diyarbakır region with some trace elements (Al, As, Ba, Be, Cd, Fe, Mn, Pb, Sb, Sn, Se, V and P) was evaluated by multivariate statistical analysis. After the soil samples were solubilized by the microwave wet digestion method, the element contents were determined with the ICP OES (Inductively Coupled Plasma Optical Emission Spectrometer) device. SRM NIST 2586 was used as SRM (Standard Reference Material) for the accuracy of the method. Recovery values were found between 91.6% and 105.9% as a result of the analysis. Humic acid was extracted from soils by the International Society for Humic Substances (IHSS) method and determined using a shaker and centrifuge device. For the accuracy of the method, it was tested with Humic Acid Sodium Salt (HA-Na). Pearson correlation and partial correlation analysis were applied to the obtained data set. In addition, multivariate statistical analyses such as multiple regression HCA (Hierarchical Cluster Analysis) and PCA (Principal Component Analysis) were applied. Multiple regression analysis was performed according to the Step-wise method. Manganese and P (p&lt; 0.01) were significant when HA was taken as the dependent variable. According to the Pearson correlation coefficient, the correlation between HA and As (r = -0.282**) in soil was negative and significant, while Fe (r = 0.185*), Mn (r = 0.273**)), Sn (r = 0.242*), Se (r = 0.325**) and P (r = 0.315**) were determined as positive and significant. In clustering and PCA analysis, HA, P Mn and Fe were found to be in the same group. The analyses have shown that HA has a positive effect on the plant nutrients in the soil.

Layer-by-layer assembled graphitic carbon nitride membranes for water treatment

Meeting societal demand for potable water supply remains one of the prioritized challenges faced in the modern era. The anthropogenic intervention has led to a dire situation threatening ecological balance and human health. There is an inevitable need for the development of new technologies and innovations in existing technologies for water treatment. Photocatalytic Membrane technology, encompassing the merits of membrane filtration and photocatalytic degradation has evolved as a potential and reliable technology for sustainable water treatment. Innovations in photocatalytic materials and membrane fabrication techniques can lead to the goal of commercialization of membrane water treatment technology. Herein, we demonstrate the potential of graphitic carbon nitride (g-C3N4) and its functionalized analog as photocatalytic membranes for sustainable water treatment. g-C3N4 and Tetracarboxyphenylporphyrin sensitized g-C3N4 (g-C3N4/TCPP) was introduced onto commercial nylon membrane surface via a layer-by-layer (LBL) assembly method using chitosan and sodium salt of polystyrene sulphonic acid as polyelectrolytes. The fabricated membranes were characterized to ensure the integration of the photocatalysts. The performance of the membranes for water treatment was assessed by selecting some common dyes as model pollutants. The modified membranes exhibited excellent flux recovery and could afford high rejection rates upon irradiation indicating the prospects for sustainable filtration.

A Wearable Electrochemical Biosensor Utilizing Functionalized Ti3C2Tx MXene for the Real-Time Monitoring of Uric Acid Metabolite.

Wearable, noninvasive sensors enable the continuous monitoring of metabolites in sweat and provide clinical information related to an individual's health and disease states. Uric acid (UA) is a key indicator highly associated with gout, hyperuricaemia, hypertension, kidney disease, and Lesch-Nyhan syndrome. However, the detection of UA levels typically relies on invasive blood tests. Therefore, developing a wearable device for noninvasive monitoring of UA concentrations in sweat could facilitate real-time personalized disease prevention. Here, we introduce 1,3,6,8-pyrene tetrasulfonic acid sodium salt (PyTS) as a bifunctional molecule functionalized with Ti3C2Tx via π-π conjugation to design nonenzymatic wearable sensors for sensitive and selective detection of UA concentration in human sweat. PyTS@Ti3C2Tx provides many oxidation-reduction active groups to enhance the electrocatalytic ability of the UA oxidation reaction. The PyTS@Ti3C2Tx-based electrochemical sensor demonstrates highly sensitive detection of UA in the concentration range of 5 μM-100 μM, exhibiting a lower detection limit of 0.48 μM compared to the uricase-based sensor (0.84 μM). In volunteers, the PyTS@Ti3C2Tx-based wearable sensor is integrated with flexible microfluidic sweat sampling and wireless electronics to enable real-time monitoring of UA levels during aerobic exercise. Simultaneously, it allows for comparison of blood UA levels via a commercial UA analyzer. Herein, this study provides a promising electrocatalyst strategy for nonenzymatic electrochemical UA sensor, enabling noninvasive real-time monitoring of UA levels in human sweat and personalized disease prevention.

Electrospun Composite PLLA-PPSB Nanofiber Nerve Conduits for Peripheral Nerve Defects Repair and Regeneration.

Peripheral nerve injury (PNI) is a common clinical problem and regenerating peripheral nerve defects remain a significant challenge. Poly(polyol sebacate) (PPS) polymers are developed as promising materials for biomedical applications due to their biodegradability, biocompatibility, elastomeric properties, and ease of production. However, the application of PPS-based biomaterials in nerve tissue engineering, especially in PNI repair, is limited. In this study, PPS-based composite nanofibers poly(l-lactic acid)-poly(polycaprolactone triol-co-sebacic acid-co-N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt) (PLLA-PPSB) are aimed to construct through electrospinning and assess their in vitro biocompatibility with Schwann cells (SCs) and in vivo repair capabilities for peripheral nerve defects. For the first time, the biocompatibility and bioactivity of PPS-based nanomaterial are examined at the molecular, cellular, and animal levels for PNI repair. Electrospun PLLA-PPSB nanofibers display favorable physicochemical properties and biocompatibility, providing an effective interface for the proliferation, glial expression, and adhesion of SCs in vitro. In vivo experiments using a 10-mm rat sciatic nerve defect model show that PLLA-PPSB nanofiber nerve conduits enhance myelin formation, axonal regeneration, angiogenesis, and functional recovery. Transcriptome analysis and biological validation indicate that PLLA-PPSB nanofibers may promote SC proliferation by activating the PI3K/Akt signaling pathway. This suggests the promising potential of PLLA-PPSB nanomaterial for PNI repair.

Simultaneous HPLC method development for azelastine HCl and fluticasone propionate nasal spray formulation

Abstract: Azelastine HCl and Fluticasone Propionate nasal spray drug product is commonly used to treat moderate to severe allergic rhinitis and rhino-conjunctivitis. To date, a simultaneous HPLC method for active sub-stances and impurities of this product has not been reported. Acetonitrile and buffer (Octane-1-Sulfonic Acid Sodium Salt and Potassium Dihydrogen Phosphate) were used as a mobile phase. The separation was achieved using Phenomenex Phenyl-hexyl (150 cm × 4.6 mm, 5 µm) column at 1 mL/min flow rate in gradient elution mode. The chromatograms were monitored at 240 nm. The validation parameters such as linearity, limit of quantification, limit of detection, accuracy, precision, robustness and specificity were investigated for the nasal spray. Overall, this study provides valuable insights into the development and validation of an efficient analytical method for assessing the quality and stability of a combination of Azelastine HCl and Fluticasone Propionate nasal spray.

Analisis Natrium Benzoat dan Rhodamin B Pada Saos Tomat Pasaran yang Beredar di Kota Gorontalo

Sodium benzoate is the sodium salt of benzoic acid which is often used in food ingridiends, for example in tomato sauce. In foodstuffs, sodium benzoate will break down into its active from or benzoate acid. However, the use of sodium benzoate should not exceed 0,1% in food ingedients. Meanwhile, Rhodamin B is a textle and pape day where this substance is very harmful if inhald, in contact with skin. In eyes and swallowed. The research aimed to determine the amount of sodium benzoat and rhodamin B in tomato sauce circulating in Gorontalo City. The samples of tomato sauce were obtained randomly from three markets in Gorontalo City regardless of its conditions whether those who did not have circulation permit or did not have obvious composition and BPOM permit. The method for determinating sodium benzoate and rhodamine B levels was carried out using UV-Vis spectrophotometry. The results obtained were sodium benzoate preservative levels in the three samples were 0,9363 g/kg in sample A, 1,5469 g/kg in sample B, and 1,8725 g/kg in sample C. Meanwhile, the rhodamine B levels in the three samples were 0,7515 in sample A, 1,5015 in sample B, and 1,5833 in sample C. Based on the results of this concentration calculation, it is observable that all samples have exceeded the threshold for using sodium benzoate and rhodamine B.

Treatment with sodium butyrate induces autophagy resulting in therapeutic benefits for spinocerebellar ataxiatype 3.

Spinocerebellar ataxia type 3 (SCA3, also known as Machado Joseph disease) is a fatal neurodegenerative disease caused by the expansion of the trinucleotide repeat region within the ATXN3/MJD gene. Mutation of ATXN3 causes formation of ataxin-3 protein aggregates, neurodegeneration, and motor deficits. Here we investigated the therapeutic potential and mechanistic activity of sodium butyrate (SB), the sodium salt of butyric acid, a metabolite naturally produced by gut microbiota, on cultured SH-SY5Y cells and transgenic zebrafish expressing human ataxin-3 containing 84 glutamine (Q) residues to model SCA3. SCA3 SH-SY5Y cells were found to contain high molecular weight ataxin-3 species and detergent-insoluble protein aggregates. Treatment with SB increased the activity of the autophagy protein quality control pathway in the SCA3 cells, decreased the presence of ataxin-3 aggregates and presence of high molecular weight ataxin-3 in an autophagy-dependent manner. Treatment with SB was also beneficial invivo, improving swimming performance, increasing activity of the autophagy pathway, and decreasing the presence of insoluble ataxin-3 protein species in the transgenic SCA3 zebrafish. Co-treating the SCA3 zebrafish with SB and chloroquine, an autophagy inhibitor, prevented the beneficial effects of SB on zebrafish swimming, indicating that the improved swimming performance was autophagy-dependent. To understand the mechanism by which SB induces autophagy we performed proteomic analysis of protein lysates from the SB-treated and untreated SCA3 SH-SY5Y cells. We found that SB treatment had increased activity of Protein Kinase A and AMPK signaling, with immunoblot analysis confirming that SB treatment had increased levels of AMPK protein and its substrates. Together our findings indicate that treatment with SB can increase activity of the autophagy pathway process and that this has beneficial effects invitro and invivo. While our results suggested that this activity may involve activity of a PKA/AMPK-dependent process, this requires further confirmation. We propose that treatment with sodium butyrate warrants further investigation as a potential treatment for neurodegenerative diseases underpinned by mechanisms relating to protein aggregation including SCA3.

Spectrophotometric Quantification of Atomoxetine Hydrochloride Based on Nucleophilic Substitution Reaction with 1,2-Naphthoquinone-4-Sulfonic Acid Sodium Salt (NQS).

A simple, sensitive, selective, and cost-effective colorimetric method has been established for the quantitative estimation of atomoxetine hydrochloride in bulk and formulation.A simple, sensitive, selective and cost effective colorimetric method has been entrenched for the quantitative estimation of Atomoxetine hydrochloride in bulk and formulation. It was established based on the visible reaction between atomoxetine hydrochloride and 1,2-naphthoquinone-4-sulfonic acid sodium salt in a basic medium (potassium hydroxide). The resulting orange colored chromogen exhibited an absorption maximum at 474 nm. Based on the optimization studies, distilled water as the solvent, 1% w/v potassium hydroxide (2 mL), and 0.3% w/v 1,2-naphthoquinone-4-sulfonic acid sodium salt (2 mL) were used in the method. The developed method was validated per the International Council for Harmonization (ICH) guidelines. The linearity was found at a concentration of 10-50 μg/mL. The method showed a good correlation between the concentration of atomoxetine hydrochloride and its absorbance. The correlation coefficient (r2) of 0.999 evidenced the same. The limits of detection and quantification were 0.20 and 0.606 μg/mL, respectively, for atomoxetine hydrochloride. The accuracy and precision of the method were also evaluated and the results obtained were within the acceptance criteria (relative standard deviation % < 2.00). The percentage assay of atomoxetine hydrochloride proved to be 101.52, which is in accordance with its label claim. The developed method is non-complex and can be effectively employed in the analytical practices of atomoxetine hydrochloride in pharmaceutical dosage forms.

Global transcriptome analysis of Pseudomonas aeruginosa NT06 response to potassium chloride, sodium lactate, sodium citrate, and microaerophilic conditions in a fish ecosystem.

Pseudomonas aeruginosa is an opportunistic pathogen that recently has been increasingly isolated from foods, especially from minimally processed fish-based products. Those are preserved by the addition of sodium chloride (NaCl) and packaging in a modified atmosphere. However, the current trends of minimizing NaCl content may result in an increased occurrence of P. aeruginosa. NaCl can be replaced with potassium chloride (KCl) or sodium salts of organic acids. Herein, we examined the antimicrobial effects of KCl, sodium lactate (NaL), sodium citrate (NaC), and sodium acetate (NaA) against P. aeruginosa NT06 isolated from fish. Transcriptome response of cells grown in medium imitating a fish product supplemented with KCl and KCl/NaL/NaC and maintained under microaerophilic conditions was analysed. Flow cytometry analysis showed that treatment with KCl and KCl/NaL/NaC resulted in changed metabolic activity of cells. In response to KCl and KCl/NaL/NaC treatment, genes related to cell maintenance, stress response, quorum sensing, virulence, efflux pump, and metabolism were differentially expressed. Collectively, our results provide an improved understanding of the response of P. aeruginosa to NaCl alternative compounds that can be implemented in fish-based products and encourage further exploration of the development of effective methods to protect foods against the P. aeruginosa, underestimate foodborne bacteria.

Effect of calcium-coacervate infiltration of artificial enamel caries lesions in de- and remineralizing conditions

ObjectivesCalcium-coacervate emulsions (CC) might be considered as mineral precursors to foster remineralization of carious dental hard tissues. This study analyzed the instant effect of repeated infiltration of artificial caries lesions with a CC emulsion as well as the effects of subsequent exposure of CC-infiltrated lesions to demineralizing and remineralizing environments. MethodsBovine enamel specimens were partly covered with varnish to leave three exposed windows. Artificial enamel caries lesions were created (pH 4.95, 17d). Baseline controls (BL) were obtained by preparing a thin section of each specimen. Specimens were allocated to five groups. In three groups lesions were etched with 37 % phosphoric acid gel, infiltrated with dipotassium hydrogen phosphate and subsequently with a calcium coacervate emulsion, prepared by mixing CaCl2 ⋅ 2H2O with polyacrylic acid sodium salt (PAA-Na). Subsequently, the infiltration effect was either analyzed immediately (Inf.) or after exposition to either de- (Inf.+DS) or remineralizing solution (Inf.+RS) for 10 or 20 days, respectively. In two control groups specimens were exposed to either DS or RS, respectively without prior CC infiltration. Integrated mineral loss [ΔZ(vol%×µm)] was analyzed using transverse microradiography (TMR). ResultsInfiltration of enamel caries lesions with coacervate solution resulted in only subtle immediate mineral gain even if repeated. When exposed to demineralizing conditions, infiltrated lesions showed significantly less mineral loss compared to untreated controls (p < 0.05; Kruskal Wallis) and exhibited characteristic mineral depositions within the lesion body. ConclusionsWhile immediate mineral gain by infiltration was only modest, the CC-emulsion might be able to prevent demineralization in acidic conditions. Clinical significanceCalcium coacervates might act protective against further demineralization when infiltrated into enamel caries lesions.

ACETYL-COA PRODUCTION BY OCTANOIC ACID ALLEVIATES ACUTE COMPARTMENT SYNDROME-INDUCED SKELETAL MUSCLE INJURY THROUGH REGULATING MITOPHAGY.

Background: Treatment of acute compartment syndrome (ACS)-induced skeletal muscle injury remains a challenge. Previous studies have shown that octanoic acid is a promising treatment for ACS owing to its potential ability to regulate metabolic/epigenetic pathways in ischemic injury. The present study was designed to investigate the efficacy and underlying mechanism of octanoic acid in ACS-induced skeletal muscle injury. Methods: In this study, we established a saline infusion ACS rat model. Subsequently, we assessed the protective effects of sodium octanoate (NaO, sodium salt of octanoic acid) on ACS-induced skeletal muscle injury. Afterward, the level of acetyl-coenzyme A and histone acetylation in the skeletal muscle tissue were quantified. Moreover, we investigated the activation of the AMP-activated protein kinas pathway and the occurrence of mitophagy in the skeletal muscle tissue. Lastly, we scrutinized the expression of proteins associated with mitochondrial dynamics in the skeletal muscle tissue. Results: The administration of NaO attenuated muscle inflammation, alleviating oxidative stress and muscle edema. Moreover, NaO treatment enhanced muscle blood perfusion, leading to the inhibition of apoptosis-related skeletal muscle cell death after ACS. In addition, NaO demonstrated the ability to halt skeletal muscle fibrosis and enhance the functional recovery of muscle post-ACS. Further analysis indicates that NaO treatment increases the acetyl-CoA level in muscle and the process of histone acetylation by acetyl-CoA. Lastly, we found NaO treatment exerts a stimulatory impact on the activation of the AMPK pathway, thus promoting mitophagy and improving mitochondrial dynamics. Conclusion: Our findings indicate that octanoic acid may ameliorate skeletal muscle injury induced by ACS. Its protective effects may be attributed to the promotion of acetyl-CoA synthesis and histone acetylation within the muscular tissue, as well as its activation of the AMPK-related mitophagy pathway.

Preparation of flower-like molecularly imprinted polymers based on metal coordination for selective extraction of hemoglobin

In this study, we present a facile strategy to prepare flower-like molecularly imprinted polymer (CuS/MIPs) for highly specific separation of hemoglobin (Hb) under neutral conditions. Cu2+ were first grafted on the surface of polydopamine-modified flower-like hollow CuS. Then, template proteins were immobilized on the Cu2+-modified CuS through coordination interaction. Subsequently, an imprinted layer was formed using acrylamide (AM) and 4-styrene sulfonic acid sodium salt (SSS) as functional monomers. After the template removal process, the cavities that can specific bind Hb were obtained. The effects of metal ions, functional monomers, pH on the adsorption capacity of the CuS/MIPs were investigated. Benefiting from the combination of multiple binding and imprinting effects, the CuS/MIPs not only exhibited large adsorption capacity (182.74 mg/g) and fast adsorption kinetics (60 min) toward template protein, but also showed satisfactory reusability and selectivity. Meanwhile, the CuS/MIPs could selectively capture Hb from bovine serum sample, demonstrating that the CuS/MIPs will be an auspicious candidate for protein separation.

Water-soluble macromers based on 2-acrylamido-2-methyl-1-propanesulfonic acid sodium salt (Na-AMPS) for rapid in situ hydrogel film formation

A novel macromer for rapid hydrogel formation is synthesized, which reduces the gelation time from 1200 s to 10 s through UV-initiated crosslinking.

Efficacy of three bioinsecticides for control of Colorado potato beetle on potatoes

An experiment was conducted to evaluate the effectiveness of three commercial bioinsecticides based on the spores of Bacillus thuringiensis var. thuringiensis. The control areas were areas where no protective measures were applied. The data showed that all bioinsecticides tested were highly effective against first, second and third instar larvae of the Colorado potato beetle Leptinotarsa decemlineata. The drug “Bitoxibacillin” ensured 100% death of larvae on day 8 at a drug concentration of 10 g/l. Combined preparation “Bitoxibacillin + GUMI” containing spores of Bacillus thuringiensis var. thuringiensis and sodium salts of humic acids, led to 100% death of larvae on the 7th day of observation. As the dosage of the drug was reduced to 5 mg/L, its effectiveness decreased to a 92% mortality rate within 9 days. The complex drug “Bitoxibacillin + Actarofid” contributed to 100% death of Colorado potato beetle larvae of all ages on the 7th day of observation at a concentration of 10 g/l. During the same time interval, 82% and 94% of the larvae died at drug concentrations of 2 mg/l and 5 mg/l, respectively.

ИЗУЧЕНИЕ ДЕЗИНФИЦИРУЮЩИХ СВОЙСТВ СРЕДСТВА «ХЛОРАПИН» ДЛЯ ДЕЗИНФЕКЦИИ В ЦЕХЕ САНИТАРНОГО УБОЯ ПТИЦЫ

The research results have been presented on «Chlorapin» disinfecting properties that has been made at the base of dichloroisocyanuric acid sodium salt. Usage this disinfectant has been proved to be effective with 0,05% (in active chlorine) concentration with 0,5 l/m2 consumption and 20 min exposition for disinfection in poultry sanitary slaughtering department lowering microbial contamination to normal traits and E. coli Inactivation.

SYNTHESIS OF TETRAHYDRATED Na (I) SALT OF 4-CARBOXY 2-NITRO TEREPHTHALATE AND ITS STRUCTURAL STUDY

An acid sodium salt of 2-nitro-terephthalic acid was synthesized and its molecular and crystal structures were studied. The crystallographic parameters of the new compound are as follows: the chemical formula – C8H8NNaO8, Cambridge Crystallographic Database Code: 2332574, molecular weight – 269.14, the temperature during measurement of the crystal parameters – 100.0 K, monocrystalline dimension 0.18mm×0.21mm×0.15mm, Radiation CuKα 𝜆= 1.54184, the minimum value of theta 4, 0250, the maximum value of theta 749580, the number of obtained reflections 9169, the number of independent reflections 2040, syngony - triclinic, space-group P-1, the specific gravity of the monocrystal d= 1.716 g/cm3 , unit cell parameters – a = 6.5034 Å, b = 7 .4080 Å, c= 11.1553 Å, the volume of the crystal lattice V=520.79 Å3 , the number of molecules in the lattice Z=2, the axial angles in the triclinic system α = 92.6990°, β= 100.1720°, γ= 99.0840°, final R factor = 0.0267. Metallic sodium ionically coordinates with the ligand. Distance between atoms – Na(1)–O(1)=2,3357 Å, Na (1)–O(4)=2,3236 Å, Na(1)–О(7)= 2,3855 Å.

The effect of plant growth regulators on the growth, development, and incidence of fungal diseases of black currant in conditions of the city of Moscow

Small-plot field experiments were laid on the territory of the Educational and Experimental Farm of the FSBEI HE RSAU-MAA named after K.A. Timiryazev on the territory of the city of Moscow. The following plant growth regulators were used in the experiments: Zavyaz, KRP (5.5 g/l gibberellic acid sodium salts); Obereg, R (0.15 g/l arachidonic acid), Zircon, R (0.1 g/l hydroxycinnamic acids), Epin-Extra, R (0.025 g/l 24-epibrassinolide). As part of the study, for two years, young blackcurrant plantings of three varieties with different maturation periods were treated with plant growth regulators. During the growing season, plant growth and development were recorded, the development of fungal diseases was recorded – powdery mildew (Sphaerotheca mors-uvae) and blister rust (Cronartium ribicola). Accounting for the yield of berries in various experiment options was carried out in comparison with the control version. The results of the study showed that the double use of growth regulators Zavyaz, KRP, Obereg, R, Zircon, R, and Epin-Extra, R positively influenced the formation of growth of black currant shoots and significantly reduced the development of fungal disease - powdery mildew on the leaves and berries of this crop.

Rapid detection of microalgae cells based on upconversion nanoprobes.

The quantification of microalgae cells is crucial for the treatment of ships' ballast water. However, achieving rapid detection of microalgae cells remains a substantial challenge. Here, we develop a new method for rapid and effective detection of microalgae concentration by utilizing upconversion nanoprobes (UCNPs) of NaYF4:Er3+,Tm3+. Three ligands, carboxylated methoxypolyethylene glycols with 5000 and 2000 molecular weights (mPEG-COOH-5, mPEG-COOH-2) and D-gluconic acid sodium salt (DGAS), were used to convert hydrophobic UCNPs into a hydrophilic state through modification. The results show that the mPEG-COOH-5 modified UCNPs present the highest stability in an aqueous solution. Fourier Transform Infrared Spectroscopy (FTIR) measurements reveal the presence of a significant number of -COOH functional groups on UCNPs after the mPEG-COOH-5 modification. These -COOH groups enhance the hydrophilicity and biocompatibility of UCNPs. The soluble UCNPs were directly mixed with microalgae, and the upconversion luminescence (UCL) spectra of the UCNPs were recorded immediately after thorough shaking. This greatly reduces the measurement time and could realize rapid onboard detection. In this sensing procedure, the UCNPs with red UCL functioned as energy donors, while microalgae with red absorption served as an energy acceptor. The UCL gradually diminishes with an increase in microalgae concentration based on the inner filter effect, thus establishing a relationship between UCL and microalgae concentration. The accuracy of the detection is further validated through the traditional microscope counting method. These findings pave the way for a novel rapid strategy to assess microalgae concentration using UCNPs.

Lipid/polyelectrolyte complexes - effects of the polyelectrolyte architecture on the self-assembled structures.

Self-assembly is a key process in forming biological materials. Especially the interaction between amphiphiles and polyelectrolytes has been widely investigated in recent years due to their potential application in industry and medicine, with a special focus on gene therapy. Accordingly, we investigated the formation of lipoplexes by mixing the cationic lipid DOTAP (1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)) with different anionic polyelectrolytes (PE), such as NaPA (sodium polyacrylate), CMC (sodium carboxymethyl cellulose) with different degrees of substitution (DS, namely, different charge density), PSS (sodium polystyrenesulfonate) and DNA (deoxyribonucleic acid sodium salt). The goal of this project was to explore the influence of different system parameters, such as the charge ratio, CR = [+]/[-] = [DOTAP]/[PE], the charge density of the PE, or the type of PE on the morphology of the formed complexes. The investigation of these systems was performed by cryo-transmission electron microscopy (cryo-TEM), and with small-angle X-ray scattering (SAXS), to support our findings. In our experiments, we obtained a comprehensive picture of the formed lipoplexes, and how their structure depends on the different properties of the employed polyelectrolyte. Although the basic nanostructure of all complexes is lamellar, their detailed morphology depends strongly on parameters of the PE, e.g., the persistence length, charge density, or the polymer backbone. Understanding these specific interactions will allow the formation of more stable and optimized complexes as they are needed for drug or genetic material delivery.