Acid-base Resistant Zone Research Articles

The Effect of a Deproteinizing Pretreatment on the Bonding Performance and Acid Resistance of a Two-step Self-etch Adhesive on Eroded Dentin.

This study evaluated how deproteinization using sodium hypochlorite (6% NaOCl) or hypochlorous acid (50 ppm HOCl) with or without the subsequent use of an arylsulfinate salt-containing agent (Clearfil DC Activator; DCA; Kuraray Noritake Dental) affects the micro-tensile bond strength (μTBS) and formation of an acid-base resistant zone (ABRZ) of a two-step self-etch adhesive on eroded dentin. Coronal dentin surfaces of sound human molars were exposed to 48 cycles of demineralization (1% citric acid; 5 minutes) and remineralization (buffer solution with pH=6.4; 3.5 hours). They were then assigned to experimental groups according to the pretreatment used: none (negative control), NaOCl, NaOCl+DCA, HOCl, and HOCl+DCA. Sound dentin surfaces with no pretreatment were used as a positive control. The dentin surfaces were bonded with Clearfil SE Bond 2 (Kuraray Noritake Dental), and μTBS was measured either after 24 hours or 20,000 thermal cycles (TC). The μTBS data were statistically analyzed using a mixed-model analysis of variance (ANOVA) and t-tests with Bonferroni correction. Failure mode was determined with scanning electron microscopy (SEM), which was also used for the observation of ABRZ. Among experimental groups, there was no significant difference between the negative control, HOCl, and HOCl+DCA after 24 hours, but the HOCl-pretreated groups exhibited significantly higher μTBS than the negative control after TC (p<0.01). Pretreatment with NaOCl and NaOCl+DCA resulted in significantly higher μTBS (p<0.001), but the highest μTBS was measured on sound dentin (p<0.001). TC decreased μTBS significantly in all groups (p<0.001) except for sound dentin and NaOCl+DCA (p>0.05). Adhesive failures prevailed in eroded groups, whereas cohesive failures were predominant on sound dentin. ABRZ was recognized in all groups but marked morphological differences were observed. The combined use of 6% NaOCl and the arylsulfinate salt-containing agent partially reversed the compromised bonding performance on eroded dentin, while the effect of 50 ppm HOCl was negligible.

Resin Cement/Enamel Interface: A Morphological Evaluation of the Acid-Base Resistant Zone, Enamel Etching Pattern, and Effect of Thermocycling on the Microshear Bond Strength.

To evaluate the effects of etching mode (self-etch and etch-and-rinse) on acid-base resistant zone (ABRZ) formation at the resin cement/enamel interface and enamel etching pattern, as well as the effects of thermocycling (0, 5000, and 10,000 cycles) on the enamel microshear bond strength (μSBS) mediated by dual-cure resin cements (DCRC). Two DCRC were used in 4 groups: Panavia V5 in self-etch (V5NE) and etch-and-rinse mode (V5E); and Estecem II in self-etch (ENE) and etch-and-rinse mode (EE). For ABRZ observation, the bonded interface was subjected to a demineralizing solution. The morphological attributes of the interface and etching patterns were observed using FE-SEM. For μ-SBS, cylinders with a 0.79-mm internal diameter and 0.5-mm height were made with DCRC and tested in shear after 0, 5000, and 10,000 thermal cycles (TC) (5°C and 55°C) (n = 10). The formation of an enamel ABRZ was observed in all groups with different morphological features between self-etch and etch-and-rinse groups. A funnel-shaped erosion beneath the interface was present using V5NE and ENE modes where enamel was dissolved, while ABRZ formation was confirmed and no funnel-shaped erosion was noticed using V5E and EE. No significant differences in μSBS were observed between resin cements. However, significantly lower µSBSs were recorded when the self-etching mode was used. Thermocycling resulted in a significant reduction in µSBS for all groups. Selective enamel etching should be recommended to improve the interfacial quality when dual-cure resin luting cements are used.

Acid-Base Resistant Zone in Teeth with the Direct Restoration Using Different Adhesive System Generations: A Systematic Review

Acid-Base Resistant Zone in Teeth with the Direct Restoration Using Different Adhesive System Generations: A Systematic Review

Can a New HEMA-free Two-step Self-etch Adhesive Improve Dentin Bonding Durability and Marginal Adaptation?

A new two-step self-etch adhesive (2-SEA) free of hydrophilic monomers was evaluated. Its microtensile bond strength (µTBS) to dentin under various aging conditions, interfacial gap formation, water sorption/solubility, and formation of an acid-base resistant zone (ABRZ) were evaluated and compared with a gold-standard 2-SEA. The new 2-SEA G2-Bond Universal (G2; GC) was compared to Clearfil SE Bond 2 (CSE2, Kuraray Noritake). Their µTBS to sound coronal dentin was tested after 1 week, 10,000 thermal cycles (TC), 20,000 TC, 6 months and 1 year. Failure mode was determined using scanning electron microscopy (SEM). Gap formation at the interface of 2-mm deep tapered cavities with an enamel border was observed using swept-source optical coherence tomography after 1 day, 1 week, 10,000 TC, and 20,000 TC. In addition, water sorption and solubility of the bonding agents was measured, and ABRZ formation was evaluated using SEM. There was no significant difference in µTBS between G2 and CSE2 (p > 0.05), and the aging conditions had no significant effect on µTBS (p > 0.05). In all groups, cohesive failures prevailed (55%-95%). Interfacial gap formation was initially similar for both adhesives (p > 0.05), but G2 exhibited a significantly lower gap formation than CSE2 after TC (p < 0.05). The water sorption of G2 was significantly lower compared to CSE2 (p < 0.05), and their solubility was statistically similar (p > 0.05). ABRZ of similar thickness was observed with both adhesives. The new 2-SEA exhibited stable dentin bonding and increased hydrophobicity resulting from the absence of hydrophilic monomers.

Fluoride-Releasing Self-Etch Adhesives Create Thick ABRZ at the Interface.

A fluoride-releasing adhesive system is expected to promote mineralization of demineralized dentin/enamel around a composite restoration, thereby contributing to the longevity of the restoration. Scanning electron microscopic (SEM) and transmission electron microscopic (TEM) observations revealed the formation of an “acid–base resistant zone” (ABRZ) beneath the hybrid layer when dentin was treated with a self-etch adhesive system. A thicker ABRZ was formed from the upper slope to the end of the outer lesion, indicating greater resistance against an acid-base challenge, when a fluoride-releasing self-etch adhesive system was used. The slope formation of a fluoride-releasing adhesive is believed to be due to fluoride-release from the adhesive. Quantitative assessment of the acid resistance was performed at the interface using the region of interest (ROI) mode of a digital image analysis software package. The area of the ABRZ is influenced by the concentration of fluoride release from the adhesive resin. The threshold of fluoride concentration in the adhesive may exist to influence the morphology of the ABRZ. X-ray absorption fine structure (XAFS) analysis of the dentin treated with different concentrations of NaF-mouth rinses suggested that different fluoride concentrations result in the formation of different chemical compounds, such as fluorapatite and CaF2-like structures, on the dentin surface. This may explain the differences in μTBS values and morphological appearance of the ABRZ. NaF is effective in enhancing the enamel/dentin bond durability and also helps create a high quality of ARBZ to improve the clinical success of restorations.

Influence of primer contamination on the bonding interface of enamel pre-etched with phosphoric acid.

In this study, the influence of primer contamination on enamel bonding was analyzed. Adper Scotchbond Multi-Purpose (SMP), CLEARFIL SE Bond 2 (SE2), Scotchbond Universal (SBU) Adhesive, and Scotchbond Etchant (35% phosphoric acid; PA) were used. Ground bovine enamels were divided into eight groups based on the bonding protocols. The bonding interfaces after an acid-base challenge were observed via scanning electron microscopy to determine the acid-base resistant zone (ABRZ). Moreover, the bonding interfaces after the nanoleakage challenge were analyzed via energy-dispersive X-ray spectroscopy. ABRZ was observed in all the samples except PA_ONLY (applied PA etching, No primer and No adhesive resin). The funnel-shaped erosion was detected only in X_SBU (applied SBU without PA etching). The nanoleakage test revealed silver patterns in some groups. The nanoleakage, i.e., the penetration of the silver ions, was detected in the groups wherein primer was applied on PA-pre-etched enamel.

Effect of repair systems on dentin bonding performance.

The purpose of this study was to investigate the influence of phosphoric acid etching, metal primer or silane coupling agent application on dentin bonding performance of a 2-step self-etch adhesive system. Human dentin surfaces were prepared and divided into four groups depending on the pre-treatment method: no pretreatment (Clearfil SE Bond 2, CONTROL); 40% phosphoric acid (K-Etchant GEL, K); metal primer (Alloy Primer, AP); or silane coupling agent (Porcelain Bond Activator, PB). The specimens were sectioned into beams, separated into 2 subgroups (n=20): no thermocyling or thermocycled (TC), and subjected to a µTBS test and an acid-base challenge for SEM observation. Statistically lower results were observed between K and AP groups, PB and CONTROL, and PB with/without TC (p<0.05). SEM images at the dentin-adhesive interface revealed that all groups produced an acid-base resistant zone (ABRZ), with a funnel-shaped erosion under the ABRZ for the K group.

Ultra-morphological studies on enamel-universal adhesive interface

ObjectivesNowadays, the universal adhesives are often used for silane application prior to application of self-adhesive resin cements. The purpose of this study was to evaluate enamel surface roughness and to observe the enamel-adhesive interface after acid-base challenge using three different self-adhesive resin cements combined with universal adhesives.Materials and Methods: Three self-adhesive resin cements: PANAVIA SA Luting Cement Plus (SA), Calibra Universal (CA) and MaxCem Elite Chroma (MC) which in conjunction with the particular universal adhesives: Clearfil Universal Bond Quick (UQ), Prime&Bond Universal (PB) and Optibond Universal (OB) were tested. Thirty enamel surfaces from caries-free human premolars were ground and bonded with the tested self-adhesive resin cements combined with universal adhesives. The surface roughness test (Sa) with or without applying adhesives was performed with 3D-CLSM. The interface of the bonded specimens after acid-base challenge was also examined by SEM. ResultsThe Sa of OB was significantly higher than those of PB and UQ. There were statistically significant differences among all of the groups (p < 0.05). An acid-base resistant zone (ABRZ) was observed in all groups, however, formation of the ABRZ was material dependent. ConclusionOB provided most etching performance to enamel of human premolars and MC group with OB presented durability against acid-base challenge. Clinical significanceNowadays, the combination of self-adhesive resin cement and universal adhesive may be a viable option for a reliable bonding performance and bonding durability in indirect restorative dentistry.

The concept of super enamel formation -Relationship between chemical interaction and enamel acid-base resistant zone at the self-etch adhesive/enamel interface.

Self-etch adhesive (SEA) systems simplify bonding procedures. It is believed that good mechanical retention and chemical bonding are both important factors to obtain reliable bonding to enamel. The enamel acid-base resistant zone (ABRZ) has been confirmed at the adhesive/enamel interface by scanning electron microscopic observation. However, the formation of enamel ABRZ is influenced by the type and components of adhesive systems. Chemical interactions by a functional monomer with hydroxyapatite strongly support the explanation of the mechanism for enamel ABRZ formation. The ABRZ created by MDP-containing SEA systems has shown good potential to resist acid attack from simulated recurrent caries models. In addition, fluoride release may enhance acid resistance. It has been proposed that such a reinforced enamel be termed "Super Enamel". The concept of the formation of super enamel may contribute to protecting enamel, and conserve tooth structure leading to improved restoration longevity.

Effect of two bleaching regimens on enamel bonding performance.

This study evaluated the post-bleaching bonding performance of a one-step and two-step self-etch adhesive (SEA) to bleached enamel using in-office or at-home bleaching. Bovine enamel surfaces were treated with an in-office or at-home bleaching agent and divided into four post-bleach time periods: immediate, one day (1d), one week (1w) and one month (1m) before application of SEA systems. Enamel was bonded using the following procedures: Group 1: one-step SEA application (BeautiBond Multi, BBM), Group 2: two-step SEA application (FL-Bond II, FLBII). Microshear bond strengths (µSBS) were measured 24-h after bonding for each of the post-bleaching time periods. Acid-base resistant zones (ABRZs) were observed under SEM. Data were analyzed using three-way ANOVA. Bleaching caused a significant decrease of the immediate µSBSs for both adhesives (p≤0.005) then increased. Thick ABRZs were observed in BBM and FLBII after bleaching. Enamel µSBSs were adversely affected by bleaching. Thick ABRZ was created with bleaching.

Adhesion durability of dual-cure resin cements and acid–base resistant zone formation on human dentin

ObjectivesBond durability and resistance to acid-base challenge of dentin with dual-cure resin cement have been rarely investigated. The purpose of this study was to evaluate dentin bond strength and observe the adhesive-dentin interface after acid-base challenge using three different dual-cure resin cements. MethodsThirty dentin surfaces from caries-free human premolars were ground and bonded with one of three dual-cure resin cements: PANAVIA V5 (V5), ESTECEM II (E II) and Rely X Ultimate (RXU) and thermocycled for 0, 5,000 and 10,000 cycles. A microtensile bond strength (μTBS) test was undertaken and the interface of the bonded specimens after acid-base challenge was examined by SEM. ResultsThe μTBS of V5 exhibited a stable bond strength despite thermal cycling while the bond of EII and RXU after 10,000 cycles dropped significantly among all thermal cycle periods (p<0.05). An acid-base resistant zone (ABRZ) was observed in all groups, however, the morphology of the bonded interface differed among the tested cements. ConclusionBonding durability to dentin and ABRZ morphologies differed among the cements evaluated. PANAVIA V5 cement system with tooth primer provided the most reliable bond strength and was best able to resist the acid–base challenge.

10-MDP Based Dental Adhesives: Adhesive Interface Characterization and Adhesive Stability-A Systematic Review.

The incorporation of functional monomers in dental adhesive systems promotes chemical interaction with dental substrates, resulting in higher adhesion forces when compared to micromechanical adhesion only. The 10-MDP monomer, whose chemical structure allows for a polar behavior which is favorable to adhesion, also promotes the protection of collagen fibers through the formation of MDP-calcium salts. This systematic review aimed to characterize the interface created by 10-MDP containing adhesive systems through an evaluation of the following parameters: Formation of nano-layered structures, capacity to produce an acid-base resistant zone, and adhesion stability. The research was conducted using PubMed, Cochrane Library, Web of Science and Embase, limited to English, Spanish, and Portuguese articles. The research was done according to the PICO strategy. The 10-MDP monomer has the capacity to produce an acid-base resistant zone on the adhesive interface, which increases the response to acid-base challenges. The adhesion established by these systems is stable over time. To have the best of these adhesive solutions, a scrubbing technique must be used to apply the adhesive system on dental substrates, in order to improve monomers infiltration and to create a stable bond. Time must be given for the solution to infiltrate, hybridize and form the MDP-Ca, improving adhesive stability.

Effects of Different Tooth Conditioners on the Bonding of Universal Self-etching Adhesive to Dentin.

To investigate the effect of different etching products used prior to the application of universal self-etching adhesive on dentin bonding. The etching products Multi Etchant (ME), Enamel Conditioner (EC), and K-etchant GEL (KE) were used as pre-treatments prior to the application of the one-bottle self-etching adhesive Adhese Universal (AU). Ground human dentin surfaces were randomly divided into four treatment groups: 1. control (CT; AU without con-ditioner); 2. ME; 3. EC; 4. KE. Microtensile bond strength (µTBS) tests with a thermocycling challenge (0-TC or 10,000-TC) were carried out and the morphological attributes of the adhesive-dentin interface were observed after an acid-base challenge using SEM to observe the acid-base resistant zone (ABRZ). The KE group had a statistically significantly lower µTBS than did the ME and EC groups at 0-TC, and than the CT, ME, and EC groups at 10,000-TC (p < 0.05). An ABRZ was observed in all groups but had partially disappeared in KE. A funnel-shaped area of erosion was also observed at the junction of the dentin and bonding layer in the CT, ME, and EC groups, while the hybrid layer was thicker in the KE group with no evidence of erosion. The application of ME or EC did not reduce the microtensile bond strength and resulted in ABRZ formation, while the ABRZ was partially reduced in the KE group.

Effect of Office Bleaching on Enamel Bonding Performance.

To evaluate the effect of in-office bleaching on enamel microshear bond strength (µSBS) of two self-etch adhesives (SEA). For the bleaching group, bovine enamel surfaces were treated with a bleaching agent and divided into two main groups: one in which adhesive was applied immediately after bleaching and another in which adhesive was applied after one week. Enamel was bonded using the following procedures: group 1: application of a two-step SEA (Clearfil SE Bond 2, SE2, Kuraray Noritake); group 2: etching with 40% phosphoric acid (PA) and application of SE2;.group 3: application of a one-step SEA (G-Premio Bond, GP, GC); group 4: etching with PA and application of GP. Resin composite was placed and µSBS was measured at 24 h (immediately) and 1 week after bleaching. Acid-base resistant zones (ABRZ) were observed under SEM. Three-way ANOVA was used for data analysis. Bleaching significantly decreased the µSBSs even after PA etching with both SE2 and GP (p ≤ 0.005). However, there were no significant differences in µSBS between groups with and without PA etching, except for SE2 without bleaching, in which the µSBS significantly increased with PA etching (p < 0.005). Thick ABRZs were found in SE2 and GP after bleaching, which exhibited an irregular wave-like shape for PA-treated specimens. The enamel µSBSs were adversely affected by in-office bleaching. Thick ABRZs were created with bleaching and PA treatment.

Influence of orthodontic self-etch adhesive on acid resistance of surface enamel.

The purpose of this study was to investigate the formation of acid-base resistant zone (ABRZ) of enamel surface during bracket bonding by using three self-etch adhesives presently available in the orthodontic treatment. Also, the relation between the thickness of the ABRZ and bonding strength, the relation between the thickness of the ABRZ and the concentration of fluoride ions contained in the adhesives, were discussed respectively. The ABRZ was formed with all self-etch orthodontic adhesives. ABRZ thicknesses of two self-etch adhesives were approximately 0.8-1.0 μm, whereas ABRZ thickness of other one was about 0.1 μm. The bond strengths of all self-etch orthodontic adhesives indicated over 10 MPa, and ABRZ thickness observed in the enamel surface seemed to have no influence to the bond strengths among self-etch adhesives. Moreover, the thickness of ABRZ appeared to be associated with the amount of fluoride ion released from the primer.

Effects of Selective Phosphoric Acid Etching on Enamel Using "No-wait" Self-etching Adhesives.

To evaluate the effect of selective phosphoric acid etching on enamel using "no-wait" self-etching adhesives. Clearfil Universal Bond Quick (UBQ, Kuraray Noritake) or G-Premio BOND (GPB, GC) was applied to ground human enamel surfaces. The adhesives were used in 3 modes: no-waiting self-etching mode (UBQ-0 or GPB-0), 10-s self-etching mode (UBQ-10 or GPB-10), and the selective-etch mode with phosphoric acid etching (UBQ-PA or GPB-PA). After an acid-base challenge, the morphological attributes of the interface were examined using scanning electron microscopy (SEM) to characterize the acid-base resistant zone (ABRZ). Microshear bond strength (µSBS) testing to enamel and ultimate tensile strength (UTS) of the adhesive resin were carried out. ABRZ formation was confirmed in all groups. UBQ-PA and GPB-PA created thicker ABRZ with no funnel-shaped erosion beneath the adhesive-enamel interface. µSBS of UBQ-PA was significantly higher than UBQ-0 and UBQ-10 (p < 0.05). However, there were no statistically signficant differences in µSBS among GPB-0, GPB-10, and GPB-PA. For the UTS, UBQ was significantly higher than GPB. Selective phosphoric acid etching created a stable adhesive-enamel interface. The no-waiting self-etching concept adhesive led to a thinner ABRZ. The results of µSBS suggest that phosphoric acid etching effects on enamel are material dependent.

Evaluation of MDP and NaF in Two-step Self-etch Adhesives on Enamel Microshear Bond Strength and Morphology of the Adhesive-Enamel Interface.

To evaluate the roles of MDP and NaF in the bonding resin of a two-step self-etch adhesive (2-SEA) on enamel bonding performance. The experimental 2-SEAs were composed of an MDP-containing self-etching primer and four different bonding resins which were MDP- and NaF-free (00), MDP-containing and NaF-free (M0), MDP-free and NaF-containing (0F), and MDP- and NaF-containing (MF) adhesives. Microshear bond strength (µSBS) to enamel and ultimate tensile strength (UTS) of the adhesive resin were determined. The morphological characteristics of the adhesive-enamel interface after acid-base challenge were observed using SEM to evaluate the acid-base resistant zone (ABRZ). No decrease in μSBS was observed even after thermocycling (TC) in 00, 0F and MF. However, the μSBS of M0 significantly decreased after TC. The UTS of M0 significantly decreased after 1- and 180-day storage in water. SEM observations indicated that ABRZ was present in all groups. However, formation of erosion beneath the ABRZ was observed in M0 and 00, whereas formation of a slope without erosion was observed at the bottom of the ABRZ in MF. In the experimental 2-SEAs, the addition of MDP alone to the adhesive resin did not contribute to an improvement in enamel bonding durability and interfacial morphology, whereas the addition of NaF in the adhesive resin demonstrated an enhancement of the bonding durability and improvement of acid resistance at the bonded interface.

Sodium hypochlorite effects on dentin bond strength and acid-base resistant zone formation by adhesive systems

Sodium hypochlorite effects on dentin bond strength and acid-base resistant zone formation by adhesive systems

Evaluation of adhesion of a CPP–ACP modified GIC to enamel, sound dentine, and caries-affected dentine

Objectives: This study evaluated the adhesion of a conventional (Fuji VII) and a recently introduced CPP–ACP containing glass-ionomer cement (Fuji VII EP) to human enamel, sound dentine, and caries-affected dentine (CAD). Material and Methods: Twenty caries-free human molars and 10 molars exhibiting frank dentine caries lesions were used. Caries removal was performed with conventional rotary method guided with a caries detector dye. The tooth surfaces were conditioned with Dentin Conditioner and then bonded with GIC restorative materials. The microtensile bond strengths were measured and analysed statistically using two-way ANOVA at the 95% confidence level. In addition, the micromorphological patterns of the GIC/tooth interfaces were observed using SEM.Results: Two-way ANOVA showed that the bond strength was not significantly affected by the type of GIC used and tooth substrate. The micromorphological analysis of the GIC/tooth interface revealed that the GICs adapted well to the tooth substrate surfaces and exhibited acid-base resistant zones on the ‘normal’ dentine but was variable on the enamel and caries-affected dentine, with the Fuji VII EP showing acid–base resistance on caries-affected dentine. Conclusions: The results demonstrate that incorporation of CPP–ACP into GIC has no adverse effect on the adhesion to enamel, sound dentine, and caries-affected dentine. It was concluded that the modified GIC can bond to commonly encountered tooth substrates.

Dentin bonding performance using Weibull statistics and evaluation of acid-base resistant zone formation of recently introduced adhesives.

Dentin bonding durability of recently introduced dental adhesives: Clearfil SE Bond 2 (SE2), Optibond XTR (XTR), and Scotchbond Universal (SBU) was investigated using Weibull analysis as well as analysis of the micromorphological features of the acid-base resistant zone (ABRZ) created for the adhesives. The bonding procedures of SBU were divided into three subgroups: self-etch (SBS), phosphoric acid (PA) etching on moist (SBM) or dry dentin (SBD). All groups were thermocycled for 0, 5,000 and 10,000 cycles followed by microtensile bond strength testing. Acid-base challenge was undertaken before SEM and TEM observations of the adhesive interface. The etch-and-rinse method with SBU (SBM and SBD) created inferior interfaces on the dentin surface which resulted in reduced bond durability. ABRZ formation was detected with the self-etch adhesive systems; SE2, XTR and SBS. In the PA etching protocols of SBM and SBD, a thick hybrid layer but no ABRZ was detected, which might affect dentin bond durability.