Acetylcholinesterase Enzyme Activity Research Articles

Potentiality of some botanical extracts as biopesticides against the maize weevil, Sitophilus zeamais Motsch. (Coleoptera: Curculionidae)

Laboratory studies were conducted to investigate the efficacy of some botanicals in the management of Sitophilus zeamais Motsch infesting stored sorghum. The selected plant species were Euphorbia balsamifera Aiton, Lawsonia inermis L. and Mitracarpus hirtus L. Chloroform leaf extracts of the botanicals applied at 6.25, 12.50, 25.00, 50.00 and 100.00 mg ml-1 were used to determine their effect on adult survival, the concentration of total body protein and inhibition of acetylcholinesterase enzyme (AChE) activity in the weevils at 30 ± 2 oC and 70 ± 5% R.H. The botanicals resulted in adult mortalities ranging from 10.00 ± 0.00 to 90.00 ± 5.77%, reduced body protein and significantly inhibited the activity of AChE in S. zeamais. The botanicals tested could serve as biopesticides in the management of S. zeamais infesting sorghum in the storage.

Effect of Beta-Cyfluthrin Pesticide on Zebra Mussel (Dressienna polymorpha)

In this study, some biochemical responses of Beta-Cyfluthrin (β-CF), which is a commercial insecticide in Dreissena polymorpha, were investigated. The 96 hour LC50 value of β-CF on D. polymorpha was calculated as 509.62 µg L-1. D. polymorpha was exposed to subletal concentrations (1/16, 1/8 and 1/4 of LC50 value: 32, 64 and 128 µg L-1) of β-CF for 24 and 96 hours. Malondialdehyde (MDA) and reduced glutathione (GSH) levels and Acetylcholinesterase (AChE) enzyme activities were determined in D. polymorpha individuals. In D. polymorpha exposed to β-CF, MDA levels increased compared to control and with increasing concentration. It was determined that GSH level decreased and AChE activity was inhibited compared to control and with increasing concentration.
 In conclusion, exposure to β-CF resulted in increased oxidative damage and has been found to cause neurotoxicity even at low concentrations.

Herbal snuff (AK-47 and HAM) induce oxidative stress and increase acetylcholinesterase enzyme activity in rat brain

Snuff has resurfaced not only in western countries but in Africa including Nigeria. It is now almost generally acceptable, among young and old in Nigeria. This research was designed to investigate the Effect of Hajiya Aisha Manpower (HAM) and AK-47 on Antioxidant Status and Acetylcholinesterase enzyme activity (AchE) of Wister Albino Rats. Thirty (30) Wister rats (110-120 g) were arbitrarily divided into five groups. Group1 (control); received only distilled water. Groups 2 and 3 (received 6 mg and 3 mg/kg b.w.t of HAM respectively). Groups 4 and 5 (received 6 mg and 3 mg/kg b.w.t AK-47 of respectively). After two months of treatments, the rats were anesthetized, blood samples were taken through heart puncture and brains of all rats were isolated and homogenized. The Result revealed Non-significant decrease in Superoxide dismutase (SOD), glutathione peroxidase (GPx) activities and concomitant increase in GSH levels in treatment groups were observed in relation to the control. While a substantial increase (p˂ 0.5) in MDA was detected in treatment groups. Brain AchE activity increased significantly in all treatment groups in relation to the control. We conclude that Both AK-47 and HAM at high concentration induce oxidative stress, decreased antioxidant enzyme activities and promote degradation of acetylcholine in rat brain homogenate.

Herbal snuff (AK-47 and HAM) induce oxidative stress and increase acetylcholinesterase enzyme activity in rat brain

Herbal snuff (AK-47 and HAM) induce oxidative stress and increase acetylcholinesterase enzyme activity in rat brain

BIOCHEMICAL INVESTIGATIONSOF THE EFFECT OF XANAX ON THE CEREBELLAR TISSUES OF MALE MICE

Xanax is an agent with hypnogenic, anxiolytic, anticonvulsant, and muscle relaxant properties and has generally been used as a hypnotic/tranquilizer. The aim of this paper was to investigate the effect of Xanax on acetylcholinesterase and glutathionestransferase enzyme activities on the cerebellar tissues of male mice. Sixty male mice were randomly assigned into four groups (15 mice/each) according to their approximately equal mean body weight. Mice that received orally by gavage 0.5 ml saline solution of 0.9% NaCl were considered as a control mice. Other experimental mice were daily administered orally by gavage with 0.5 ml of different three doses of Xanax (0.5, 1 and 1.5 mg/kg bw), for two months. Biochemical analyses revealed significant decreases in the activities of acetylcholinesterase and glutathionestransferase enzyme in the brain tissues of mice administered with the three doses of Xanax. The major mechanism involved appears to be the result of oxidative stress scavenging action on the neuronal cells of cerebellar cortex of male mice.

ACETYLCHOLINESTERASE, AS A POTENTIAL BIOMARKER OF NAPHTHALENE TOXICITY IN DIFFERENT TISSUES OF FRESHWATER TELEOST, ANABAS TESTUDINEUS

Naphthalene, a Polycyclic Aromatic Hydrocarbon is widely used as a fumigant and disinfectant despite its toxic effect and is ranked as the ninth most threatening compound. The present study was carried out to determine the in vivo effect of naphthalene at different concentrations on acetylcholinesterase (AChE) enzyme activity in different tissues of Anabas testudineus. The fishes were exposed to varying concentrations of naphthalene (4.2 mgL–1, 4.4 mgL–1, 4.6 mgL–1, 4.8 mgL–1 and 5 mgL–1) for a period of 72 hours. Acetylcholinesterase enzyme activity was found to be significantly inhibited, in a dose-response manner. The inhibition percentage of AChE activity varied from 9.34–43.95% in brain tissue, 2.56–35.81% in liver tissue, 5.94–34.15% in muscle tissue and 3.92–33.75% in gills in comparison to the tissues of the control group. Maximum inhibition of acetylcholinesterase enzyme activity in treated fish was observed in the brain followed by liver, muscles, and gills. This study highlights the significance and role of acetylcholinesterase as a potential stress biomarker of naphthalene toxicity.

Anti-Alzheimer potential of a waste by-product (peel) of Omani pomegranate fruits: Quantification of phenolic compounds, in-vitro antioxidant, anti-cholinesterase and in-silico studies

Alzheimer's disease (AD) is an irreversible progressive neurodegenerative disease. Punica granatum L., ( P. granatum ) is one of the most important commercial crops of Oman with traditional medicinal claims. The study aimed to quantify the phenolic content of the Omani pomegranate peel extracts and to investigate their antioxidant, anti-cholinesterase activities along with computational studies for the development of AD therapy. The dried peels of P. granatum fruits were exhaustively extracted with chloroform, ethyl acetate and butanol. Peel extracts were subjected to the preliminary phytochemical screening. Colorimetric methods were used to quantify total phenolic and flavonoids contents. The neuroprotective potential of the peel extracts was evaluated by assessing the in-vitro antioxidant activity and their ability to inhibit acetylcholinesterase (AChE) enzyme activity. In-silico analysis and molecular docking of the most promising phenolic metabolite present in pomegranate peel was also carried out. The phytochemical screening showed the presence of flavonoids, tannins, alkaloids, carbohydrates and terpenoids. The total phenolic, flavonoids content & antioxidant activity was found to be higher in the butanol extract. Surprisingly, butanol extract showed slightly lower AChE inhibitory activity than chloroform extract. Catechin, a polyphenolic compound present in the peel showed lower binding energy for AChE in comparison to Beta-secretase-1 (BACE-1). It formed three and two hydrogen bonds with AChE and BACE-1, respectively. The results of the current study provided evidence that butanol extract is rich in phenolics and possesses excellent antioxidant activity. Furthermore, in silico and in-vitro studies conducted on P. granatum revealed promising anti-AD activity. Further investigations must be carried out to isolate and develop the chemical constituents of pomegranate peel as neuroprotective agent(s). • Phytochemical screening and quantification of phenolic compounds in an agro-waste. • Evaluation of antioxidant and anticholinesterase activity of pomegranate peel extracts. • In silico studies and molecular docking studies on the polyphenolic compounds found in pomegranate peel. • Butanol solvent was found to be optimum for the isolation of polyphenolic compounds. • Pomegranate peel could be used as an alternative source of natural antioxidants.

Nicotiana rustica L’nin Bazı Önemli Metabolik Enzimleri Üzerindeki İnhibisyon Etkisi, LC-MS / MS Analizi, Antioksidan Özellikleri

Being widely used since its discovery, tobacco contains many chemical substances including nitric oxide, hydrocarbons, aldehydes, phenols, quinone and semiquinone radicals, and causes the formation of oxygen-induced free radicals directly or indirectly. Therefore, it is important to know the oxidant/antioxidant properties, phenolic content of Nicotiana rustica Linn., and the effect studies on Paraoxonase (PON 1) and Acetylcholinesterase (AChE) which are important metabolic enzymes. In the study, the effect of extracts (water and methanol) of Nicotiana rustica Linn on PON 1 and AChE enzyme activity was examined. Water extract of Nicotiana rustica Linn Maras Powder with walnut, (MPWW) and methanol extract Maras Powder with Nicotiana rustica, (MPNR), Maras Powder with walnut, (MPWW) and Maras Powder with copper cauldron, (MPCC) had an inhibitory effect on AChE (IC50 values in the range of 0.104-0.203 mg ml-1). Also water extract MPCC and methanol extract MPNR, MPWW and MPCC showed an inhibition effect on PON 1 (IC50 values 0.013-0.225 mg ml -1). MPNR water extract showed the highest activity with 26% DPPH radical scavenging activity. MPCC water extract showed a 13% ABTS radical scavenging activity.

High nutritional status promotes vitality of honey bees and mitigates negative effects of pesticides

Honey bee health is affected by multiple stressors, such as the exposure to plant protection products (PPPs), dietary limitation, monofloral diets and pressure of diseases and pathogens and their interactions. Here, we analysed the interacting effects of plant protection products and low nutritional pollen source on honey bee health under semi-field conditions. We established a healthy honey bee colony in each of 24 tents, planted either with monofloral maize, maize with a diverse flower strip or with monofloral Phacelia tanacetifolia. To evaluate the interaction between exposure to PPPs and nutritional status, a mixture of the insecticide thiacloprid and the fungicide prochloraz was applied. For each colony, we investigated brood capping rate as well as adult longevity, body and head weight, and enzyme activity of acetylcholinesterase and P450 reductase of newly hatched worker bees.We found a significant reduced capping rate in treated maize compared to flowering strips and Phacelia, but no interaction effect between pesticide treatment and nutritional status on capping rate. The response to treatment on the longevity of adults differed significantly between maize and Phacelia, with flower strips being intermediate, indicating interaction effects of PPP treatment and low pollen quality in maize compared to Phacelia and flowering strip treatments.Head weight of newly hatched worker bees showed significant interaction of nutritional status and treatment of PPPs. PPPs slightly increased body weight in all nutritional statuses, except for Phacelia. Enzyme activity of acetylcholinesterase and P450 reductase showed significant different responses between maize and Phacelia to PPP exposure, but not between maize and flowering strip.Our results support the hypothesis that higher pollen quality promotes development of larvae and pupae, longevity of adults and detoxification of PPPs.

Ameliorative Potential of Glycyrrhiza glabra Extracts on Memory Impairments in Stress Triggered Rats

Background: Glycyrrhiza glabra Linn. (Family: Fabaceae) is known to be a very useful medicinal plant in the Traditional Medicinal Systems since centuries. With ethnopharmacological values, it is a well-reported plant for its anti-inflammatory, antioxidant, anxiolytic, expectorant and antidepressant activities. Objective: Although the plant has been described to possess memory enhancing activity, the present study focused on examining the comparative effect of Glycyrrhiza glabra extracts viz. flavonoid rich (GGFE) and glycyrrhizin rich (GGGE) in stress-triggered rats and providing future research insights on this herbal drug, for which no scientific justification has been reported till now. Methods: Male Wister rats divided into 7 different groups (n= 6 per group) were given chronic foot-shock stress for 21 successive days with scheduled administration of the extracts (50 and 100 mg/kg) and standard drug (10 mg/kg) for 28 days. Elevated Plus Maze, Rectangular Maze, Morris Water Maze, and Locomotor activity were performed to test behavioral alteration and learning ability of stressed rats. Further, rats were sacrificed to assay acetylcholinesterase activity and antioxidant activity in brain samples for their mechanistic role in learning and memory. Results: Extracts of Glycyrrhiza glabra indicated a significant alteration in stress-induced learning and memory deficiency in behavioral parameters studied. These extracts also modulated significant changes in acetylcholinesterase and antioxidant enzyme activity to improve the learning and memory of stressed rats. Conclusions: Both the extracts of Glycyrrhiza glabra (GGFE and GGGE) were found to exert memory enhancing property in stress-triggered rats. Moreover, the comparative results confirmed that the high potential of GGGE in comparison to GGFE might be due to the rich glycyrrhizin content present in GGGE responsible for acetylcholinesterase and antioxidant enzyme modulatory activities. Therefore, GGGE could be used as a promising lead for further mechanistic and molecular studies for defining the role of glycyrrhizin in Glycyrrhiza glabra.

Neuroprotective Potential of Mung Bean (Vigna radiata L.) Polyphenols in Alzheimer's Disease: A Review.

Mung bean contains various neuroprotective polyphenols, so it might be a healthy food for Alzheimer's disease (AD) prevention. Totally, 19 major phenolic compounds were quantified in mung bean, including 10 phenolic acids and 9 flavonoids. After summarizing their contents and effective doses in rodent AD models, it was speculated that vitexin, isovitexin, sinapic acid, and ferulic acid might be the major bioactive compounds for mung bean-mediated neuroprotection. The mechanisms involved inhibition of β-amyloidogenesis, tau hyperphosphorylation, oxidative stress, and neuroinflammation, and promotion of autophagy and acetylcholinesterase enzyme activity. Notably, the neuroprotective phenolic profile in mung bean changed after germination, with decreased vitexin and isovitexin, and increased rutin, isoquercitrin, isorhamnetin, and caffeic acid detected. However, only studies of individual phenolic compounds in mung bean are published at present. Hence, further studies are needed to elucidate the neuroprotective activities and mechanisms of extractions of mung bean seeds and sprouts, and the synergism between different phenolic compounds.

Developmental toxicity assessment of 4-MBC in Danio rerio embryo-larval stages

Enormous production of cosmetic products and its indiscriminate use tends to discharge into the aquatic environment and might threaten non-target organisms inhabiting aquatic ecosystems. In the present study, developmental toxicity of 4-methylbenzylidene camphor (4-MBC), a widely used organic UV filter in personal care products has been evaluated using zebrafish embryo-larval stages. Waterborne exposure induced developmental toxicity and deduced 2.71 mg/L as 96 h LC50 whereas embryos exposed to sub-lethal concentrations (50 and 500 μg/L) caused a significant delay in hatching rate, heart rate, reduced larval length, and restricted hatchlings motility besides the axial curvature. Chronic exposure to 10 dpf resulted in significant decrease in SOD activity at 500 μg/L with no changes in CAT level besides a significant increase in GST enzyme at 5 μg/L concentration in 5 dpf sampled larvae. However, all the three enzymes were significantly elevated in 10 dpf larvae indicating differential oxidative stress during the stages of development. Similar trend is noticed for acetylcholine esterase enzyme activity. A concentration dependent increase in malondialdehyde content was noted in larvae sampled at 5 and 10 dpf. In addition, multixenobiotic resistance (MXR) activity inhibition, and elevated oxidative tissue damage were noticed at 5 dpf with no significant changes in 10 dpf larvae. Furthermore, immunoblot analysis confirms 4-MBC induced apoptosis in zebrafish larvae with promoted cleaved Caspase-3, Bax and inhibited Bcl-2 proteins expression. Subsequently, docking studies revealed the binding potential of 4-MBC to zebrafish Abcb4 and CYP450 8A1 proteins with the binding energy of −8.1 and −8.5 kcal/mol representing target proteins interaction and toxicity potentiation. Our results showed that 4-MBC exposure triggers oxidative stress at sub-lethal concentrations leading to apoptosis, deformities and locomotion perturbations in developing zebrafish.This is first of its kind in systematically demonstrating developmental toxicity of 4-MBC and the information shall be used for aquatic toxicity risk assessment.

Developmental nicotine exposure impairs memory and reduces acetylcholine levels in the hippocampus of mice

Nicotine is a strong psychoactive and addictive compound found in tobacco. Use of nicotine in the form of smoking, vaping or other less common methods during pregnancy has been shown to be related to poor health conditions, including cognitive problems, in babies and children. However, mechanisms of such cognitive deficits are not fully understood. In this study we analyzed hippocampus dependent cognitive deficits using a mouse model of developmental nicotine exposure. Pregnant dams were exposed to nicotine and experiments were performed in one month old offspring. Our results show that nicotine exposure did not affect locomotor behavior in mice. Hippocampus dependent working memory and object location memory were diminished in nicotine exposed mice. Furthermore, acetylcholine levels in the hippocampus of nicotine exposed mice were reduced along with reduced activity of acetylcholinesterase enzyme. Analysis of transcripts for proteins that are known to regulate acetylcholine levels revealed a decline in mRNA levels of high affinity choline transporters in the hippocampus of nicotine exposed mice but those of vesicular acetylcholine transporter, choline acetyltransferase, and α7-nicotinic acetylcholine receptors were not altered. These results suggest that developmental nicotine exposure impairs hippocampus dependent memory forms and this effect is likely mediated by altered cholinergic function.

Neuroprotective Effect of Fucoxanthin against Intracerebroventricular Streptozotocin (ICV-STZ) Induced Cognitive Impairment in Experimental Rats.

Alzheimer's disease (AD) is a neurological disorder characterized by loss of memory and cognitive functions caused by oxidative stress, neuroinflammation, change in neurotransmitter levels, and excessive deposition of Aβ(1-42) plaques. Fucoxanthin is a carotenoid with potential antioxidant, anti-inflammatory, and neuroprotective actions. In the present study, fucoxanthin was employed as a protective strategy in Intracerebroventricular Streptozotocin (ICV-STZ) induced experimental model of cognitive impairment. STZ was injected twice ICV (3 mg/kg) on alternate days 1 and 3, and Wistar rats were evaluated for the memory analysis using Morris water maze and elevated plus-maze. Fucoxanthin at low 50 mg/kg, p.o. and high dose 100 mg/kg, p.o. was administered for 14 days. All animals were sacrificed on day 29, and brain hippocampus tissue after isolation was used for biochemical (MDA, nitrite, GSH, SOD and Catalase), neuroinflammatory (TNF-α, IL-1β, and IL-6), neurotransmitters (ACh, GABA Glutamate), Aβ(1-42) and Tau protein measurements. STZ-infused rats showed significant impairment in learning and memory, increased oxidative stress (MDA, nitrite), reduced antioxidant defense (GSH, SOD and Catalase), promoted cytokine release, and change in neurotransmitters level. However, fucoxanthin improved cognitive functions, restored antioxidant levels, reduced inflammatory markers dose-dependently, and restored neurotransmitters concentration. The finding of the current study suggests that fucoxanthin could be the promising compound for improving cognitive functions through antioxidant, anti-inflammatory, and neuroprotective mechanisms, and inhibition of acetylcholinesterase (AChE) enzyme activities, Aβ(1-42) accumulation, and tau protein.

Improvement of memory deficits via acetylcholinesterase inhibitory activity of Nepeta nuda ssp. nuda essential oil in rats

Nepeta L. (Lamiaceae) species are belonging to aromatic and medicinal plants. Many biological activities of Nepeta species have been reported. In this study, memory-enhancing activities of Nepeta nuda ssp. nuda essential oil was aimed to be evaluated in laboratory rats treated with scopolamine. GC-MS/GC-FID system was used to identify oil composition. Memory performances of the rats were tested using Y-maze and radial-arm maze. Acetylcholinesterase enzyme activity and molecular docking analysis were also performed in this study. In total, 44 different compounds were identified in Nepeta nuda ssp. nuda essential oil. Trans-cis nepetalactone was showed to be the most abundant constituent in the oil. In the Y-maze test, the oil improved the performances of the animals on spontaneous alternation. Besides, in the radial-arm maze test, the oil improved working and reference memory significantly impaired in the rats subjected to scopolamine. The oil also showed acetylcholinesterase inhibitory activity in vitro. The molecular docking method, in the meantime, revealed that some of the compounds in the oil could interact with the acetylcholinesterase enzyme, theoretically. Nepeta nuda ssp. nuda essential oil improved spatial memory of scopolamine-induced rats. Neuroprotective effects of the oil are probably related to the anti-cholinesterase activity of the oil. Therefore, Nepeta nuda ssp. nuda essential oil could be used as a complementary therapy to improve memory in Alzheimer’s and related diseases.

Use of biomarkers in monitoring pollution status of urban rivers, Limpopo, South Africa.

This study investigated the use of enzymes and histology as potential biomarkers in Sand and Blood Rivers in Limpopo Province, South Africa. Physico-chemical parameters were analyzed at sites S1, S2, S3, S4, and S5 of the Sand River and sites B1 and B2 of the Blood River. Sites S1 and B1 were selected as the reference sites and located upstream of the Polokwane and Seshego wastewater treatment plants (WWTPs) effluent discharge points, respectively. Water quality parameters assessed were water temperature, dissolved oxygen (DO), biological oxygen demand (BOD), total dissolved solids (TDS), turbidity, ammonia, nitrogen, phosphorus, and pH. Sites downstream the WWTPs recorded elevated levels of nitrogen, phosphorus, and ammonia. DO, ammonia, nitrogen, and phosphorus were above the targeted water quality range (TWQR) for aquatic ecosystems. The Canadian council of ministers of the environment water quality index (CCME WQI) showed that the reference sites had good water condition while sites downstream (S2, B2, S3, and S4) had poor water condition. Acetylcholinesterase (AChE) enzyme activity in the brain of Clarias gariepinus from sites downstream was lower than those from sites upstream of the effluent discharge points. The liver of Clarias gariepinus from sites downstream showed higher lactate dehydrogenase (LDH) enzyme activity compared to fish caught at the reference sites. Furthermore, Clarias gariepinus from sites downstream showed a number of histological alterations in gills and liver than fish from upstream. Histological alterations observed in gills included fusion of the secondary lamellae, hyperplasia of the interlamellar, epithelial lifting of secondary lamellae, and hyperplasia of the secondary lamellae. In the liver, histological alterations observed included melanomacrophage centers (MMCs), macrovesicular steatosis, sinusoid congested with kupffer cells, nuclei pleomorphism, and vacuolation. The gill and liver indices were significantly lower at the reference sites compared to sites downstream. Evidently, AChE, LDH, and gill and liver histology can be used as early warning signs of aquatic degradation in rivers that are recipients of poorly treated sewage effluent.

Nanomaterials based biosensors for the detection of organophosphate compounds: a review

ABSTRACT The extensive use of pesticides for enhancing crop productivity is posing a serious health concern to the living beings. No doubt, agricultural productivity has been increased to many folds using pesticides but due to the presence of toxic organophosphate (OP) compound in pesticides, they have become serious threat to the society. As these compounds inhibit activity of acetylcholinesterase enzyme involved in normal functioning of body, it leads to birth defect, immature delivery, infertility, irritability, fatigue, nervousness and depression. So, it is necessary to detect the presence of these acutely toxic compounds in the food and environment samples. There are several conventional methods available such as colorimetry, capillary electrophoresis, high performance liquid chromatography (HPLC), thin layer chromatography (TLC) etc., but they are not much accurate, sensitive, time-consuming and lack selectivity. All these drawbacks are eliminated by use of nanomaterial-based biosensors, which can successfully screen the various samples for the presence of OP compounds. The review focuses on different categories of nanomaterials used for the fabrication of present-day inhibition-based biosensors using acetyl cholinesterase. In this, various properties of biosensors such as electrode material, mode of detection, detection limit, linearity range, sensitivity and selectivity are reported in tabular form. The enzyme-free sensors such as aptasensors are also discussed in the review. With the help of this review, the researchers can understand the mechanism of detection of OP compound using nanomaterials, variety of nanomaterials to develop fast and efficient detection of OP compounds.

In Vivo Effects of Neonicotinoid-Sulfoximine Insecticide Sulfoxaflor on Acetylcholinesterase Activity in the Tissues of Zebrafish (Danio rerio).

Sulfoxaflor is the first member of the neonicotinoid-sulfoximine insecticides that acts as an agonist of nicotinic acetylcholine receptors (nAChRs). This study investigated the acute effects of sulfoxaflor on acetylcholinesterase (AChE; EC 3.1.1.7) enzyme activity in the brain and muscle tissues of zebrafish (Danio rerio) as a model organism. The zebrafish were exposed to 0.87 mg/L (2.5% of 96 h 50% lethal concentration (LC50), 1.75 mg/L (5% of 96 h LC50) and 3.51 mg/L (10% of 96 h LC50) of sulfoxaflor for 24 h–48 h and 96 h periods. AChE enzyme activities were analysed by a spectrophotometric method in the brain and muscle tissues. The results of this study showed that in vivo acute sulfoxaflor exposure significantly increased AChE enzyme activity in the brain and muscle tissues of zebrafish. The induction percentages of AChE were between 10 and 83%, and 19 and 79% for brain and muscle tissues, respectively. As a result, it was found that sulfoxaflor had an effect on AChE enzyme activity in the two main tissues containing this enzyme, and it can be considered as a potential neuroactive compound for zebrafish.

Neurotoxic responses of rainbow trout (Oncorhynchus mykiss) exposed to fipronil: multi-biomarker approach to illuminate the mechanism in brain

Insecticides have potential to non-target organisms, disrupting the healthy functioning of the aquatic environment as they are the ultimate receptor of the aquatic ecosystem. Insecticides, which are widely used in agriculture, have high neurotoxicity on aquatic organisms. In this study, the acute alterations [catalase (CAT), arylesterase (ARE), malondialdehyde (MDA), myeleperoxidase (MPO), paraoxonase (PON), glutathione peroxidase (GPX), superoxide dismutase (SOD), 8-hydroxy-2-deoxyguanosine (8-OHdG) level, caspase-3 activity, and Acetylcholinesterase (AChE) enzyme activity] caused by the different concentrations of Fipronil (FP) insecticide (0.05, 0.1, and 0.2 mg/L) on rainbow trout (Oncorhynchus mykiss) brain tissue were investigated. It has been determined that superoxide dismutase –catalase - glutathione peroxidase - paraoxonase and arylesterase enzyme activities were inhibited but MDA and MPO induced depending on the concentration in brain tissue. When compared with the control group, the changes between the pesticide exposed groups were found statistically significant (p < 0.05). In brain tissue, while AChE enzyme activity was decreased depending on concentration, caspase-3 activity increased with 8-OHdG level. As a result, it has been determined that FP is a dangerous environmental pollutant for aquatic organisms, even at low concentrations, inducing oxidative stress, damaging the brain tissue of fish and stimulating apoptosis.

Neuroprotective Potential of &lt;i&gt;Bacopa monnieri, Camellia sinensis&lt;/i&gt; and Curcumin on Chlorpyrifos Exposed &lt;i&gt;Drosophila melanogaster&lt;/i&gt;

The popular treatment approaches of neurodegenerative diseases are generally for symptom management, but unfortunately, there is no significant curative approach present so far that can signifcantly reduce its progression. Indian traditional medicine is being used alone or in a combination of herbs to fight against these diseases. The commonly used herbs/traditional medicine(s) for this purpose are Bacopa monnieri (Brahmi), Curcuma longa (curcumin) and Camellia sinensis (green tea). In the present study, herbs were judged for its free radical scavenging activity through DPPH free radical scavenging assay, Total Phenolic Content (TPC) and also Total Flavonoid Content (TFC). Among them, green tea exhibited highest total phenolic content and also higher total flavonoid content (99.667±3.547 mg GA/g and with 7.633±2.532 mg QE/g, respectively) followed by Bacopa monnieri and Curcumin. In this context, Green tea showed maximum free radical scavenging activity than curcumin followed by Brahmi (GT&gt;CUR&gt;BM). These herbs were evaluated to check their potential in the improvement of the climbing ability of flies and acetylcholinesterase enzyme activities using Drosophila melanogaster (fruit flies). In this study, fruit flies were fed normal food, chlorpyrifos contaminated food and also chlorpyrifos (Chlor) along with individual herbs as mentioned above (Chlor+GT, Chlor+CUR, Chlor+BM). Interestingly, we found that the free radical scavenging activity and antioxidant activities, of Brahmi (97%) has the highest improvement in climbing activity as compared to green tea (90%) followed by curcumin (86%) and organisms treated with these herbs show a trend BM&gt;GT&gt;CUR. Moreover, Brahmi also exhibited maximum improvement in AChE activities as compared to curcumin and green tea and showed a trend BM&gt;CUR&gt;GT.