Acetic Acid Test Research Articles

In vivo and in vitro evaluation of pharmacological activities of Adenia trilobata (Roxb.)

Adenia trilobata, locally known as akandaphal in Bangladesh, has some traditional uses. Leaves and stems extracted with pure methanol (MEATL, MEATS) and fractioned by n-hexane (NFATL, NFATS), which was subjected to qualitative phytochemical analysis. The qualitative phytochemical analysis of four extracts showed the presence of secondary metabolites such as alkaloid, carbohydrate, glycosides, flavonoids, phenols, flavonol, and saponins. All four extracts of A. trilobata, exhibited a strong antioxidant activity while a moderately (MEATS = 328 μg/mL) to weakly toxic (NFATL = 616.85 μg/mL) LC50 observed in brine shrimp lethality bioassay. In thrombolytic test, MEATL (18.54 ± 2.18%; P < 0.01) and MEATS (25.58 ± 4.76%; P < 0.0001) showed significant percentage of clot lysis in human blood. The in vivo analgesic activity carried by acetic acid test and formalin test, while the antidiarrheal activity assayed by two standard methods e.g., castor oil-induced diarrhea and castor oil-induced gastrointestinal motility. Both, in vivo model, showed an extremely significant (P < 0.0001) dose-dependent manner percentage of inhibition in comparison to the control group. Present results suggested, A. trilobata could be a potential source for antioxidative, cytotoxic, thrombolytic, analgesic, antidiarrheal agents which require further study to identify the mechanism of A. trilobata.

Anti-nociceptive and anti-inflammatory effects of Ferulago angulata

Introduction: Ferulago angulata from the Apiaceae family, has high flavonoid content and is detected to have anti-nociceptive and anti-inflammatory effects. Objectives: In this study, we sought to determine the components of essential oil and to estimate total phenol and flavonoid contents of its various extracts. We also aimed to find out the anti-nociceptive and anti-inflammatory effects of essential oil, hydro-alcoholic and phenolic extracts of F. angulata aerial parts. Materials and Methods: The plant’s essential oil and extracts were prepared according to standard methods. Acetic acid, hot plate and formalin tests were used to investigate anti-nociceptive effects. Additionally, carrageenan and croton oil tests were used to evaluate anti-inflammatory effects. Results: Ferulago angulata aerial parts yielded 0.2% (v/w) yellowish essential oil. The gas chromatography/ mass spectrometry (GC-MS) of essential oil identified 82 compounds, which represented 98.9% of the essential oil. Thymol (7.9%), spathulenol (6.5%), trans-anethol (6.4%), myristicin (5.1%) and alpha-pinene (4.5%) were the main components. In acetic acid and formalin tests, the essential oil, hydro-alcoholic and phenolic extracts showed significant anti-nociceptive effects (P&lt;0.001). In hot plate test, morphine which was used as standard drug, revealed significant anti-nociceptive effect while the plant extracts and essential oil were ineffective. High dose of the extracts and essential oil in croton oil test (P&lt;0.001) and high dose of hydro-alcoholic and phenolic extracts in carrageenan test (P&lt;0.05) reduced the inflammation. Conclusion: Ferulago angulata extracts and essential oil have anti-nociceptive and anti-inflammatory effects. However, further studies are needed to clarify their mechanism of actions.

Anti-nociceptive and anti-inflammatory activities of ethanol extract and fractions of Morus mesozygia Stapf (Moraceae) leaves and its underlying mechanisms in rodents

Ethnopharmacological relevanceMorus mesozygia Stapf (Moraceae), commonly known as African mulberry, is traditionally used for the treatment of inflammatory disorders such as rheumatism and dermatitis. AimThis work aimed to evaluate the anti-nociceptive and anti-inflammatory effects of its ethanol (EEMm) extract, and ethylacetate fraction (EAFMm). MethodsThe anti-nociceptive and anti-inflammatory effect of ethanol extracts of M. mesozygia (EEMm), and its ethylacetate (EAFMm) and residual aqueous fraction (RAFMm) was evaluated in hotplate, acetic acid and formalin tests and as well in membrane stabilizing assay and carrageenan-induced paw oedema models. Mechanism of anti-inflammation of EAFMm was investigated in the carrageenan-induced air-pouch model. ResultsIn the hot plate test of nociception, only the EAFMm showed significant (p < 0.05) anti-nociceptive activity. The extract and fractions significantly reduced number of writhing with EAFMm (400 mg/kg) showing highest inhibition (66.5%) in the acetic acid-induced writhing in mice. EEMm and EAFMm (400 mg/kg) significantly reduced the paw licking time in the early and late phases of the formalin test. The extract and fractions showed good membrane stabilizing activity comparable to indomethacin. EAFMm (100 and 400 mg/kg) showed the highest inhibition of paw oedema (53.4% and 58.1%) in the carrageenan-induced paw oedema model. The EAFMm (100 and 400 mg/kg) reduced exudate volume relative to carrageenan-control (2.64 ± 0.22, 2.08 ± 0.15 vs 3.83 ± 0.18 mL) and neutrophils (8.98 ± 1.36, 8.00 ± 0.22 vs 20.51 ± 1.14) in carrageenan-induced pouch. EAFMm significantly reduced exudate volume, pro-inflammatory cytokines and the expression of COX-2 and NFκB. ConclusionM. mesozygia leaves demonstrated anti-nociceptive and anti-inflammatory activities by suppressing oxidative stress, pro-inflammatory cytokines, cyclooxygenase-2, and nuclear factor kappa B.

A Prospective Randomized Trial to Compare Safety, Acceptability and Efficacy of Thermal Ablation and Cryotherapy in a Screen and Treat Setting.

Background:The prospective randomized study aimed to compare the safety, acceptability and efficacy of thermal ablation (TA) to that of cryotherapy in screen and treat setting. Methods:The participants were recruited prospectively in a community-based screening clinic in India. Women positive on visual inspection with acetic acid (VIA) test and/or Human Papillomavirus (HPV) test were assessed for eligibility for ablative treatment. Total 286 eligible women were randomized to receive either cryotherapy (N=150) or TA (N=136) performed by health workers. Colposcopy and cervical biopsy were performed on all, prior to treatment. Post-treatment follow-up was after one year with colposcopy and biopsy. Results:Both the treatment methods had high acceptability. Significantly higher proportion of women treated by cryotherapy reported pain compared to women treated by TA, though intensity was mild in vast majority of them. Approximately 30% of women in both arms had histologic abnormalities, mainly CIN 1, and among those who attended follow-up 74.1% and 81.0% didn’t have any CIN after cryotherapy and TA respectively. Conclusion:TA is as acceptable and safe as cryotherapy in screen and treat setting. TA has the logistic advantages for the low-resourced settings as the machines are more portable, do not require costly refrigerant gas and battery-driven models are available. The cure rates for CIN 1+ lesions in our study were comparable between cryotherapy and TA.

Antinociceptive and anti-inflammatory effects of Cuphea aequipetala Cav (Lythraceae).

Cuphea aequipetala Cav (Lythraceae) is an herb used in folk treatment for pain and inflammation. The aim of this study was to evaluate the antinociceptive and anti-inflammatory actions of an ethanol extract from the leaves and stem of Cuphea aequipetala (CAE). The antinociceptive actions of CAE (10-200mg/kg p.o.) were assessed with the acetic acid-induced writhing, hot plate, and formalin tests. The possible mechanism of action of CAE was evaluated using inhibitors. The effects of CAE on motor coordination were assessed by the rotarod test. The in vitro anti-inflammatory actions of CAE were evaluated using LPS-stimulated primary murine macrophages, and the in vivo anti-inflammatory actions were assessed by the TPA-induced ear oedema and the carrageenan-induced paw oedema tests. The production of inflammatory mediators was estimated from both in vitro and in vivo assays. CAE showed antinociception (ED50 = 90mg/kg) in the acetic acid test and in the second phase of the formalin test (ED50 = 158mg/kg). Pretreatment with glibenclamide or L-NAME partially reversed the antinociception shown by the plant extract. CAE (50-200mg/kg) did not affect motor coordination in mice. CAE increased the production of IL-10 in LPS-stimulated macrophages (EC50 = 10pg/ml) and, in the carrageenan-induced paw oedema test (threefold increase). In conclusion, CAE induced antinociceptive effects without affecting motor coordination, probably due to the involvement of nitric oxide and ATP-sensitive K+ channels. CAE also exerts in vitro and in vivo anti-inflammatory effects by increasing the release of IL-10.

Thermal Ablation Versus Cryotherapy or Loop Excision to Treat Women Positive for Cervical Precancer on Visual Inspection With Acetic Acid Test: Pilot Phase of a Randomised Controlled Trial

(Abstracted from Lancet Oncol 2020;21:175–184) The most commonly used approach for women with cervical precancer in low-income and middle-income countries (LMICs) is screening by visual inspection with acetic acid (VIA) followed by immediate cryotherapy of VIA-positive women (see-and-treat). There are a number of logistical problems with cryotherapy that have prompted development and evaluation of a cordless, lightweight, and battery-operated thermal ablator that has a much faster treatment duration (20–40 seconds) and does not require a gas supply.

Thermal Ablation Versus Cryotherapy or Loop Excision to Treat Women Positive for Cervical Precancer on Visual Inspection With Acetic Acid Test: Pilot Phase of a Randomized Controlled Trial

(Abstracted from Lancet Oncol 2020;21:175–184) Cervical cancer has a long and treatable precancerous phase, is easily screened for, and is common enough to justify population-based screening even in low- and middle-income countries (LMICs). The standard treatment in high-income countries is large loop excision of the transformation zone (LLETZ).

A mechanistic approach to HPLC analysis, antinociceptive, anti-inflammatory and postoperative analgesic activities of panch phoron in mice

BackgroundPanch phoron is a mixture of five spices containing an equal proportion of Foeniculum vulgare (fennel), Trigonella foenum-graecum Linn (fenugreek), Nigella sativa (black cumin), Cuminum cyminum (cumin) and Brassica nigra (black mustard). The mixture is commonly used in Bangladeshi cuisine and possesses many pharmacological effects. In this study, we evaluated the antinociceptive and anti-inflammatory activities of aqueous panch phoron extract (PPE) in vivo, its possible mechanism of action and phytochemical analysis by High-Performance Liquid Chromatography (HPLC). We also investigated the effect of PPE on postoperative pain in mice.MethodsHPLC was carried out using LC-20A Modular HPLC system to identify the bioactive compounds present in PPE. Five groups of Swiss albino male mice (n = 6 per group) were orally treated with 10 ml/kg of distilled water or 10 mg/kg of sodium diclofenac or three doses of PPE (100 mg/kg, 300 mg/kg, 500 mg/kg). In vivo assessment was carried out by the writhing test, tail-flick test, formalin test, and carrageenan induced paw edema test. The opioid antagonist, naloxone was used in the acetic acid test to evaluate the involvement of opioid receptors. To assess the effect of PPE in postoperative pain, mice that underwent sciatic nerve surgery were measured for the paw withdrawal latency in a hot water bath.ResultsIn HPLC analysis, different types of phenolic compounds and flavonoids, including catechin hydrate, para-coumaric acid, vanillic acid, and syringic acid were detected. Treatment with PPE exhibited dose-dependent antinociceptive and anti-inflammatory activities in pain models (p < 0.05). Furthermore, naloxone did not reverse the effect of PPE in the writhing test. Mice that underwent sciatic nerve surgery showed that the paw withdrawal latency increased gradually over 7 days.ConclusionsOur results demonstrate that PPE has significant antinociceptive and anti-inflammatory activities and can provide significant postoperative analgesia.

Chrysophyllum albidum fruit peel attenuates nociceptive pain and inflammatory response in rodents by inhibition of pro-inflammatory cytokines and COX-2 expression through suppression of NF-κB activation

Chrysophyllum abidum fruit is a seasonal fruit commonly eaten as snacks with abundant health promoting phytochemicals in the fruit peels. The fruit peels have been reported to be rich in anti-inflammatory eleagnine, myricetin rhamnoside, quercetin, linoleic acid and oleic acid. We hypothesized that the anti-inflammatory effect of the peel extract involve suppression of pro-inflammatory cytokines, cyclooxygenase-2 and nuclear factor-kappa B (NF-κB). Hence, this study was designed to assess the anti-nociceptive and anti-inflammatory effects of fruit peel extract of Chrysophyllum albidum in animal models of nociception and inflammation. The anti-nociceptive activity of CAPEE (100 and 400 mg/kg) was evaluated in acetic acid-induced writhing and formalin-induced paw licking in mice. Formalin-induced paw edema and carrageenan-induced air pouch models of inflammation were used to evaluate the anti-inflammatory activity. CAPEE (100 and 400 mg/kg) significantly reduced abdominal writhing and paw licking in acetic acid and formalin tests in mice, respectively. CAPEE demonstrated significant inhibition of paw edema at 24 h (41.0% and 55.7%) and 72 h (52.3% and 86.6%) after formalin injection. CAPEE suppressed inflammatory responses in carrageenan-induced air pouch by reducing exudates, inflammatory cells infiltration, nitrites and myeloperoxidase activity. There was significant inhibition of tumor necrosis factor-alpha, interleukin-6 levels and reduced immunopositive expression of COX-2 and NF-κB. In conclusion, CAPEE has anti-nociceptive and anti-inflammatory potentials via mechanisms associated with inhibition of pro-inflammatory cytokines and cyclooxygenase-2 (COX-2) expression through suppression of nuclear factor kappa B (NF-κB) activation, and has potential as a functional food ingredient.

Characterization and phytostimulatory activity of bacteria isolated from tomato (Lycopersicon esculentum Mill.) rhizosphere

Replacing agrochemicals with plant growth promoting bacteria (PGPB) may offset some of the environmental impacts of food production. The objectives of this study were to (1) isolate and characterize bacterial strains from tomato rhizosphere, including root, shoot and leaf, (2) select and identify the most promising PGPB strains, (3) verify the phytostimulatory activity and mineral uptake potential of selected strains. Bacterial strains isolated from tomato rhizosphere, were screened for phosphorous (P) solubilization, production of indole acetic acid (IAA), amylase activity, antibiotic resistance, and quick test strip (QTS) for biochemical characterization. The tested strains, positive for all five of these assays were selected for molecular identification and subjected to greenhouse growth trails with tomato and mung bean. Two strains were selected and identified as Bacillus cereus (B. cereus) isolated from rhizosphere and Klebsiella variicola (K. variicola) isolated from root endosphere using 16s rRNA sequences. Both strains produced IAA, gibberellic acid (GA3) and kinetin, however B. cereus showed potential GA3 and IAA production as compared to K. variicola. In tomato, only one growth variable (shoot length) was increased over the control by one of the selected bacterial strains (B. cereus). In mung bean, inoculation with either strain B. cereus or K. variicola increased shoot length and dry weight. Moreover, our results showed that the use of PGPB significantly increased plant growth and Fe, Zn, Ca, Mg, Cu, Na and K contents of plants. It seems that evaluated strains had a higher ability in boosting plant growth and higher yield.

Thermal ablation versus cryotherapy or loop excision to treat women positive for cervical precancer on visual inspection with acetic acid test: pilot phase of a randomised controlled trial

Thermal ablation versus cryotherapy or loop excision to treat women positive for cervical precancer on visual inspection with acetic acid test: pilot phase of a randomised controlled trial

Hypoglycemic, Antitussive and Analgesic Effects of Nanoparticles of Cordia myxa Fruits Extract

Background: Cordia myxa is a plant from Boraginaceae family and has many useful therapeutic properties. Aim of the study is to compare the pharmacological efficacy of Cordia myxa hydro-alcoholic extract (Bulk Extract [BE]) and nanoparticles of hydro-alcoholic extract (NPE) including hypoglycemic, antitussive, anti-inflammatory and analgesic activity. Methods: Hydro-alcoholic maceration extract of dry fruits was powdered to nanoparticle size by ball-milling technique. Hyperglycemia and cough were induced in mice by alloxan (120 mg/kg, i.p.) and ammonia, respectively. Inflammatory and analgesic evaluations of extracts were carried out with the formalin-induced edema in the mice paw and acetic acid tests. NPE and BE were administrated at single dose (750 mg/kg, i.p.). Positive control group was received dextromethorphan (10 mg/kg, orally) in cough and indomethacin (25 mg/kg, orally) for analgesic models. Blood glucose level, the number of coughs and writhes and duration of foot lickings were calculated. Results: The blood glucose did not statistically reduce by extracts. Both extracts could significantly inhibit the frequency of cough and dramatically reduce the duration of paw licking. NPE could significantly reduce writhes. Conclusion: We concluded that extracts had no hypoglycemic activity. Both extracts created therapeutic activities better than dextromethorphan. NPE exerted better anti-inflammatory and analgesic activity compared to BE.

Anti-nociceptive and anti-inflammatory effect of imperatorin: evidences for involvement of COX-2, iNOS, NFκB and inflammatory cytokines

Aim: The objective of the current investigation was to explore the analgesic effect of naturally occurring furanocoumarin, imperatorin and the involvement of inducible cyclooxygenase (COX-2), inducible nitric oxide synthase (iNOS), NFκB and cytokines in the observed effect.Materials and methods: Anti-nociceptive effect was explored by inducing chemical hyperalgesia using acetic acid and formalin in mice. ED50 of imperatorin was calculated in acetic acid model. Modulation of cyclooxygenase and nitric oxide pathway by imperatorin was examined by stimulator/precursor challenge with substance P and L-arginine, respectively and quantification of COX-2, iNOS and NFκB expression by immunohistochemical analysis in spinal tissues. Involvement of inflammatory cytokines TNF-α and IL-1β was investigated using LPS challenge and subsequent ELISA analysis of these inflammatory mediators in serum. Carrageenan inflicted paw edema was employed to explore the anti-inflammatory activity of imperatorin.Results: A significant reduction in the nociceptive behaviour was observed with imperatorin treatment in acetic acid and formalin test. ED50 of imperatorin was found to be 4.53 mg/kg. Pre-treatment with substance P and L-arginine significantly attenuated the anti-nociceptive activity of imperatorin in formalin test. Immunohistochemical findings revealed marked decrease in spinal COX-2, iNOS and NFκB expression. Imperatorin administration significantly reduced LPS induced rise in level of TNF-α and IL-1β dose dependently. In carrageenan-induced paw edema test, maximum possible anti-inflammatory effect of imperatorin was evident after 240 min of carrageenan administration.Conclusion: Current investigation revealed that anti-nociceptive and anti-inflammatory potential of imperatorin is probably mediated through the attenuation of COX-2, iNOS, NFκB activity and reduction in circulatory cytokines.

Evaluation of anti-nociceptive and anti-inflammatory activities of Piper sylvaticum (Roxb.) stem by experimental and computational approaches

Piper sylvaticum Roxb., (Family: Piperaceae), commonly known as pahaari peepal, is used in traditional medicine for the treatment of rheumatic pain, headache, asthma, chronic cough, diarrhea, and wounds. To provide scientific proof for its traditional use, the present study was designed to investigate the antinociceptive and anti-inflammatory properties of methanol extract of P. sylvaticum stem (MEPSS) in pain models. Additionally, computational studies viz. molecular docking, ADME and toxicological property predictions were performed to identify the potent phytochemicals of this plant for antinociceptive and anti-inflammatory activities with good oral bioavailability and safety features. Quantitative phytochemical analysis of MEPSS was performed using established protocols. The antinociceptive activity was determined using acetic acid and formalin test in mice at the doses of 200 and 400 mg/kg while paw edema induced by carrageenan used for anti-inflammatory activity. Molecular docking study was performed by Schrödinger Maestro 10.1 whereas the SwissADME and admetSAR were used for ADME and toxicity prediction respectively. The total phenolic and flavonoid contents of MEPSS were 93.39 and 53.74 mg gallic acid and quercetin equivalent/g of extract respectively. The methanol extract exhibited significant and dose-dependent antinociceptive and anti-inflammatory effects in experimental pain models. Also, our docking study showed that piperine, piperlonguminine, and sylvamide have the best binding affinities to cyclooxygenase enzymes with good ADME/T properties. This study confirmed that MEPSS possess significant antinociceptive and anti-inflammatory activities which could be due to the presence of phytochemicals and three bioactive compounds (piperine, piperlonguminine, and sylvamide) were found to be most effective in computational studies.

THE DETERMINANTS OF CERVICAL CANCER EARLY DETECTION BEHAVIOUR WITH VIA TEST METHOD IN VISITORS OF POLI KIA-KB IN KASSI-KASSI PUBLIC HEALTH CENTER OF KOTA MAKASSAR

Background: Cervical cancer can be recognized at the pre-cancer stage by doing early detection test in married women. One of the cervical cancer early detection methods is Visual Inspection with Acetic Acid (VIA) test. The coverage of the early detection of cervical cancer through VIA test method in 2017 was still very low, i. e. 1.03% from the national target of 50%. The research aims to know the determinants of cervical cancer early detection behaviour by using IVA test method in visitors of Policlinic KIA-KB (Pediatric and Family Planning Polyclinic) in Kassi-Kassi Public Health Center.Methods: The research was observational analytical research with cross-sectional study design. The population of the study was all women of fertile couple that visited Policlinic KIA-KB in Kassi-Kassi Public Health Center and lived within the coverage area of Kassi-Kassi Public Health Center, which amounted to 135 women. The samples were 101 women that were collected by using the accidental sampling technique.Results: The results showed that there was a relation between knowledge (p=0.000), attitude (p=0.005), husband support (p=0.000), as well as health worker support (p=0.000) and early detection behaviour by using IVA test method. Conclusions: Meanwhile, there was no relation between education and early detection behaviour by using IVA test method. There should be proactive and creative actions from health workers in improving the knowledge of the targets and their husbands about early detection of cervical cancer.

Automated Detection of Cervical Cancer

Cervical Cancer is an abnormal growth in the cervix - the lower part of the uterus which joins to the vagina. Human Papilloma Virus (HPV) is identified as the main cause for cervical cancer. Cervical cancer is the second most deadly disease among women next to breast cancer in developing countries. However, it is considered to be the most preventable female cancer if identified at an early stage. The cancerous cells may spread to other parts of the body if not identified at an early stage. Pap Smear test and acetic acid test are usually done for cancer screening. In pap test cells are taken from the vagina and cervix and are examined under a microscope for the presence of an abnormal cell. In acetic acid test, the change in features after and before the application of acetic acid is analyzed to find the existence of abnormal cell. Automated screening is becoming most common than manual screening because the latter is erroneous. This paper surveys the different automated methods available for screening the abnormal cells in pap images.

Comparison of Classifier Configurations for the Classification of Cervical Intraepithelial Neoplasia Using Acetic Acid Test Images

Cervical cancer represents a major cause of death for women. Automatic classification of cervical images from acetic acid test could serve as a promising screening tool for cervical cancer. Despite an increasing volume of studies on automatic classification of cervical images, reported methods varied markedly in terms of features and classifiers used, and therefore the performance. The classification performance using different configurations of the classifier has not been well characterized. The objective of this study was to evaluate several frequently used features and classifiers in acetic-acid cervical image based cervical intraepithelial neoplasia classification. Seven typically used color or texture-based features and four frequently used classifiers (Support Vector Machine, Random Forest, Back-Propagation Neural Network and K-Nearest Neighbors) were included in the comparison based on a balanced large sample size including 175 CIN negative and 175 CIN positive patients. The results showed that the Support Vector Machine demonstrated the best classification accuracy when a subset of features was used. The finding of this study may provide useful reference values to the development of an automatic cervical cancer screening tool.

The Utility of Urine-Based Sampling for Cervical Cancer Screening in Low-Resource Settings.

Background:WHO has recommended Visual Inspection with Acetic acid (VIA) or Human Papillomavirus (HPV) DNA testing if feasible, for cervical cancer screening in low income countries. However, the number of women undergoing screening is very low as a result of limited information, inadequate infrastructure and invasive nature of sampling. Methods:A cross sectional study was carried out comparing HPV DNA detection by Polymerase Chain Reaction (PCR) in paired cervical and urine samples procured from histologically confirmed cervical cancer cases. Results:Amongst the samples collected from 114 cervical cancer cases, HPV DNA was tested positive in cervical samples of 89 (78.1%) and urine samples of 55 (48.2%) patients. The agreement between the two sampling methods was 66.7% and the kappa value was 0.35 indicating a fair agreement. The sensitivity of HPV detection using urine samples was 59.6% (95% confidence interval 49.16%-69.15%) and the specificity was 92% (95% confidence interval 75.0%-97.8%). Conclusion:Even though not acceptable as an HPV DNA screening tool due to low sensitivity, the urine sampling method is inexpensive and more socially acceptable for large epidemiological surveys in developing countries to estimate the burden.

Se - [(2,2-Dimethyl-1,3-dioxolan-4-yl) methyl] 4-chlorobenzoselenolate reduces the nociceptive and edematogenic response by chemical noxious stimuli in mice: Implications of multi-target actions.

The present study evaluated the antioxidant, antinociceptive and anti-edematogenic effects of Se-[(2,2-dimethyl-1,3-dioxolan-4-yl) methyl] 4-chlorobenzoselenolate (Se-DMC). In vitro experiments were carried out to evaluate Se-DMC antioxidant action. Thiobarbituric acid reactive species levels, 2,2'-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-thylbenzthiazoline-6-sulfonic acid) radicals scavenging and glutathione S-transferase-like activity were determined. Male Swiss mice were orally pretreated with Se-DMC (1, 10 and 50 mg/kg), meloxicam (50 mg/kg) or vehicle 30 min prior to acetic acid or glutamate test. To extend our knowledge of the pharmacological properties of this compound, it was tested in an inflammatory model through ear edema induced by croton oil. The contribution of glutamatergic and serotonergic systems was also investigated. In vitro experiments revealed that Se-DMC exerts antioxidant activity. Nociception induced by glutamate or acetic acid was reduced by Se-DMC or meloxicam. Se-DMC diminished the paw edema formation induced by glutamate, while meloxicam did not show any effect. Se-DMC and meloxicam decreased the ear edema formation and protected against the increase in myeloperoxidase activity in mice ear induced by croton oil. The pretreatment of animals with MK-801 did not alter antinociception caused by Se-DMC in the glutamate test. The antinociceptive effect exerted by Se-DMC in the acetic acid test was reverted by the pretreatment of mice with different serotonergic antagonists (WAY100635, ketanserin and pindolol). Data presented here showed that the modulation of serotonergic and glutamatergic systems and the anti-inflammatory and antioxidant actions could contribute to the antinociceptive and anti-edematogenic effects of Se-DMC and it supported the therapeutic potential of this compound.

Penetapan Kadar Asam Salisilat pada Krim Anti Jerawat yang Beredar di Kota Bandung dengan Metode Spektrotometri Ultra Violet

Salicylic acid is an anti-acne as well as keratolytic which is commonly given topically. The purpose of this study was to determine the levels of salicylic acid in anti-acne creams circulating in traditional markets, supermarkets, and skin care in Bandung, and to compare the levels of salicylic acid in samples with the maximum salicylic acid content limit determined by the Center for Drug and Food Control (BPOM). Qualitative analysis using the thin layer chromatography (TLC) method with the toluene mobile phase: acetic acid (4: 1) and color test using FeCl3 reagent. While the quantitative analysis of salicylic acid in anti-acne cream using ethanol solvent and measured by a UV spectrophotometer. Method validation to prove that the method used has reached the requirement. Salicylic acid levels in sample G were 2.33%, C 1.54%, B 0.71%, R 0.85%, and I 0.82%. Samples C, B, R and standard sample set by BPOM which is no more than 2%. Sample G does not unqualified because the level is more than 2%.