Chitosan In Acetic Acid Research Articles

The effects of Brazilian chitosan-based biomaterials on wound healing in rats

The use of natural supplies is a resource to mimic an original extracellular matrix that allows for migration, proliferation, and cellular organization. Chitosan from Brazilian Atlantic Ocean had low protein, minerals percentage and excellent antibacterial activity. The aim of this study was to evaluate and to compare the effectiveness of different types of acids as solvents with Brazilian chitosan-membrane in the healing process of skin lesions. Experimental full-thickness 2 × 2 cm wounds were created on the dorsum skin of Wistar rats. The applied different treatments were saline, collagenase®, microcrystalline chitosan salt membrane (MCSM), microcrystalline chitosan acetic acid membrane (MCAAM), and microcrystalline chitosan hydrochloric acid membrane (MCHAM). The wound repairs were measured morphologically and histologically on days 0, 3, 7, 10, and 14. The exudate formation and the final wound contractions were similar in all of the groups. There were mild exudations in the groups with chitosan-membranes, despite the formation of crust under the membrane. This configured a serum hematic aspect, but there was no impact on the healing process. The MCHAM group had more favorable aspects that histologically showed the healing phases. A significant migration of neutrophils and macrophages seen by myeloperoxidade and Beta-N-Acetylglucosaminidase activities was evident in the chitosan groups, MCHAM and MCSM, respectively. Furthermore, the MCHAM group created its histological arrangement in a dense and more consistent manner.

Thermal properties of cellulose-chitosan composite fibers

Cellulose-chitosan composite fibers were obtained by the method of wet spinning from joint solutions in phosphoric acid. Thermogravimetry (TG), differential thermogravimetry (DTG) and differential scanning calorimetry (DSC) methods have been used to evaluate their thermal properties in the range of 20-600 °C in air atmosphere. It was found that the temperature of the maximum decomposition rate of composite fibers lies between the temperatures characteristic of individual components. It is shown that the addition of chitosan to cellulose leads to an increase in the mass of the carbon residue and gives the cellulosic fibers a self-extinguishing property. The observed effect is explained by the uniform distribution of cellulose and chitosan macromolecules and their aggregates separated by interphase layers in each other, in which a net of interpenetrating segments of both polymer macromolecules is represented. This provides the incombustibility for composite fibers with a chitosan content of more than 20 % and maintaining the integrity of the fibers after processing them with a solvent of chitosan-acetic acid.

Cadmium and Copper Removal From Aqueous Solutions Using Chitosan-Coated Gasifier Biochar

Gasifier Biochar (GBC) and Chitosan-Coated Gasifier Biochar (CGBC) derived from pine wood was used to remove Cu2+ and Cd2+ from water. Chitosan-Coated Gasifier Biochar was made by mixing GBC with aqueous acetic acid chitosan solution followed by treatment with NaOH. Both CGBC and GBC were characterized using FT-IR, scanning electron microscopy, surface area measurement (BET), elemental analysis, thermogravimetric analysis, and point of zero charge. Chitosan accounts for 25% of the weight of the CGBC. Thermogravimetric analysis showed chitosan decomposes sharply at 225–270°C and then more slowly thereafter. The BET surface areas of GBC and CGBC were 34.1 and 4.61 m2/g, respectively. Batch adsorption studies performed at pH values of 2–5 followed Cu2+ and Cd2+ adsorption quantitatively using atomic absorption spectrophotometry. Sorption was evaluated using the Freundlich, Langmuir, and Sips isotherm models. Cu2+ adsorption on CGBC fit best the Sips model (capacity 111.5 mg/g) and Cd2+ with the Langmuir model (capacity 85.8 mg/g). Langmuir adsorption capacities on GBC were 83.7 and 68.6 mg/g for Cu2+ and Cd2+ respectively. CGBC removed more Cu2+(25.8 mg/g) and Cd2+(17.2 mg/g) than GBC because chitosan modification generates amine coordination sites that enhance metal adsorption. Adsorption on CGBC and GBC of both metal ions followed pseudo-second order kinetics.

Phytochemical and physiological response of Satureja hortensis L. to different irrigation regimes and chitosan application

Water stress is the most common abiotic environmental factor that influenced the morphological and physiological traits of the plants. Application of antitranspirants substances such as chitosan can reduce water loss. Summer savory (Satureja hortensis L.) is one of the important medicinal and aromatic plants which is used in food and pharmaceutical industries. In this investigation, the response of summer savory to foliar application chitosan (acetic acid, 0, 0.5, 1 and 2 g/L) under three irrigation regimes (30, 60, and 90 % field capacity (FC), as a pot factorial experiment in randomized complete design with three replications, was evaluated. Chitosan and irrigation regimes treatments had significant effects on studied parameters, including biomass, dry matter, essential oil content, and composition as well as selected physiological characteristics comprising photosynthetic pigments, osmotic adjustment, total phenolic content, and antioxidant activities of the extract of summer savory. The results showed that chitosan application was useful under severe water stress (30 % FC) and 2 g/L of chitosan improved total phenolic, total soluble sugar and proline contents, antioxidant activity, and essential oil content of the plant. Elicitation of summer savory with chitosan under water stress modified essential oil compositions such as carvacrol, γ-terpinene, and p-cymene. In conclusion, the findings of the present experiment indicated that chitosan could be a favorable material used to decrease the deleterious effect of water stress. Furthermore, the chitosan spraying can behave beneficial effects on the essential oil quality and quantity as well as osmotic adjustment, antioxidant activity, and growth of summer savory under water stress conditions.

TERMITICIDAL ACTIVITY OF CHITOSAN ON PAPER

Termites are insects that can damage buildings, paper and plants. Termites are controlled by using termiticides (chemicals). Besides polluting the environment, termiticides may have harmful effects to organisms, including humans, and destroy metal. The use of chemicals will be environmentally and economically profitless. A joint effort to utilize biodegradable material will help to reduce the negative impacts of termiticides. One of the materials that can use to control termites is chitosan. The purpose of this study was to determine the termiticidal activity of chitosan on paper against termites. The termiticidal activity test followed method JIS K 1571 2004. The resistance of paper to termite damage was determined by calculating the percentage of weight loss and termite mortality rate. The following concentrations of chitosan in acetic acid were used: 0.5%, 1%, 1.5% and 2%. The test result showed that weight loss percentages were 20.49%, 16.37%, 15.77% and 10.80%, respectively. On the other hand, the weight loss percentage of paper without chitosan was 32.69%, which shows that termites do not favour chitosan used in the paper. The activity of termites was successfully inhibited when the concentration of chitosan was increased, and the percentage of paper weight loss decreased from 10.80% to 20.49%. Paper without chitosan had a termite mortality rate of 18.3%; The addition of chitosan increased termite mortality to 28.2%–30.4%. The percentage of weight loss decreased with increasing termite mortality. The termite mortality rate show that chitosan is nontoxic and potential as biotermiticide for paper.

Detoxification Properties of Guanidinylated Chitosan Against Chemical Warfare Agents and Its Application to Military Protective Clothing.

This study investigates the detoxification properties of guanidinylated chitosan against chemical warfare agents and its application to the preparation of military protective clothing. Guanidinylated chitosan was synthesized by chitosan guanidinylation with cyanamide. The detoxification properties of the guanidinylated chitosan were then evaluated using a chemical warfare agent simulant, called diisopropylfluorophosphate (DFP). Cotton fabric was treated with 1 wt.% of guanidinylated chitosan in acetic acid and water solution using the simple and conventional textile treatment method of pad–dry–cure. The detoxification properties of the guanidinylated chitosan-treated cotton fabric were evaluated to investigate the application of guanidinylated chitosan to the preparation of military protective clothing. Subsequently, 71.3% of DFP was hydrolyzed to non-hazardous diisopropylhydrogenphosphate (DHP) in 2 h because of the base organocatalytic activity of 0.02 g guanidinylated chitosan itself. Moreover, 60.1% of DFP was hydrolyzed by the catalytic activity of the guanidinylated chitosan-treated cotton fabric, which contained only 0.0002 g of guanidinylated chitosan. This result shows that the guanidinylated chitosan itself has detoxification properties for hydrolyzing DFP to DHP, and its detoxification properties can be more efficient when applied to cotton fabric because it showed 84.3% of the detoxification properties with only 1 wt.% of guanidinylated chitosan. For the first time, this study shows that guanidinylated chitosan has considerable detoxification properties and can be used as an agent to prepare protective clothing.

Silver-loaded microspheres reinforced chitosan scaffolds for skin tissue engineering

In order to improve the mechanical properties and antibacterial properties of skin tissue engineering scaffolds, a microspheres-reinforced composite construct with controlled release property was designed. The silver-loaded chitosan (CS) microspheres (Ag@CMs) were prepared by emulsified crosslinking method. Ag@CMs were introduced into CS acetic acid solution and then Ag@CMs embedded porous CS scaffolds were fabricated by lyophilization method. The results show that Ag@CMs are uniformly distributed inside porous CS scaffolds and there is no significant effect on the structure of the pores. The Ag@CMs/CS composite scaffolds possess high porosity (66.03 ± 0.15) %. The compressive strength and modulus of the composite scaffold reach up to 0.47 ± 0.03 MPa and 3.95 ± 0.06 MPa, respectively, significantly higher than pure CS scaffold. The in vitro drug delivery tests and antibacterial activity tests illustrate that composite scaffolds have efficient antibacterial properties and excellent drug sustained-release performance. MTT assay indicates that Ag@CMs/CS porous scaffold can provide an interconnected porous structure and large surface area for mouse fibroblast cell (L929) adhesion and proliferation. These results suggest that Ag@CMs/CS porous scaffold is a promising candidate for skin tissue engineering.

Synthesis of Nano-Polymer Supported on Nano-Hydrogel Chitosan Base and Its Application for DOX Delivery

In this study, a nanogel-hydrogel hybrid was synthesized to improve drug delivery system performance. First, poly (acrylic acid-N-isopropylacrylamide) nanoparticles were synthesized using acrylic acid, N-isopropylacrylamide, sodium dodecyl sulfate, and MBA in the presence of APS as a radical initiator. Then the synthesized (A-N) nanogel was added to a solution of chitosan in acetic acid and (A-N) nanogel/ CTS-A hydrogel was synthesized in the presence of AA, MBA and ASP. (A-N) nanogel/ CTS-A hydrogel was characterized by IR, SEM, EDX, TEM, DLS, TGA, DTG and BET techniques. Doxorubicin (DOX) solution was used to evaluate drug loading capacity and the amount of drug-loaded was calculated using UV analysis. Drug release behavior was investigated by placing DOX-loaded (A-N) nanogel/ CTS-A at different temperature and pH ranges. Our research indicated the drug release for both carriers was controlled by diffusion mechanism but, increasing of temperature had not influenced the drug release mechanism. However, this study suggests that by trapping nanoparticles in a hydrogel system, drug carriers with higher absorption capacity and slower kinetic release can be created.

Formulation and in-vitro Evaluation of Oral Captopril Bioadhesive Delivery System

Abstract: Background: Captopril is considered as the drug of choice in the treatment of hypertension and congestive heart failure. It has a very short duration of action and a biological half-life of 1-2 hr. This requires its administration in 2-3 times daily which is not convenient for the management of a chronic disease like hypertension. Objectives: Captopril is unstable at the alkaline pH of the gastrointestinal tract. Therefore, it is difficult to be delivered orally in a sustained release formulation. This defect can be overcome by using bioadhesive or floating delivery systems which could increase the residence time of the drug in the stomach. Methods: Different formulations of bioadhesive chitosan microspheres containing captopril were prepared using the cross-linking method. Characterization of these microspheres was performed by measuring percent yield, particle size, swelling and bioadhesive properties, drug content and in-vitro release of captopril. Results: Chitosan microspheres prepared from 2% high molecular weight chitosan in acetic acid with 5ml glutaraldehyde and a cross-linking time of 3 hr gave the highest yield percent (83%), the highest entrapment efficiency (55%) and sustained release over 8 hr, compared with other examined formulations of chitosan. The release kinetics from all the prepared microspheres were diffusion-controlled mechanism and the drug release from microspheres was dependent on its concentration. Conclusion: In conclusion, chitosan microspheres with their particle size and release behavior seem to be a good carrier for captopril. Key words: Chitosan, Microspheres, Bioadhesion, Captopril, In-vitro release.

Effect of a multifunctional edible coating based on cassava starch on the shelf life of Andean blackberry

The blackberry is a fragile fruit with a high degree of decomposition, which limits its shelf life. The effect of an edible coating (EC) based on cassava starch, whey protein, beeswax, chitosan, glycerol, stearic acid, and glacial acetic acid on the shelf life of fruit stored at 4 °C was evaluated. The physical, chemical, physical, microbiological, and sensorial quality was evaluated, comparing with a fresh control fruit. The EC had a positive effect on the physicochemical and sensorial properties (mainly in texture, flavor, and aromas), due to the reduction of physiological processes, whereas the color changes are mainly due to anthocyanin losses. After 10 days of storage, weight losses were 39.6% lower and firmness was 81.4% higher; while chitosan reduced the mold and yeast count. The EC increased the useful life of the Andean blackberries by 100%.

Preparation of Coated Corrugated Box for Controlled-Release of Chlorine Dioxide and Its Application in Strawberry Preservation

Chlorine dioxide (ClO2) has received great attention as a nontoxic and efficient antimicrobial agent for the preservation of fresh fruits and vegetables. A novel two-layer coated corrugated box was developed to release gaseous ClO2 under the trigger of moisture in this study. The inner surface of the box was firstly coated with a mixture of polyvinyl alcohol-NaClO2-diatomite and then with chitosan acetic acid solution. Results showed that ClO2 was successfully released under high humidity due to the reaction of NaClO2, water vapor and acid. The concentration of released ClO2 increased with the increasing NaClO2 content in the coating, while the addition of diatomite stabilized and extended the release. To evaluate the preservation effect, strawberries were packed in the coated box and stored at room temperature. Compared with the control, the decay rate and weight loss of the strawberries packed in the coated box (9 g/L NaClO2) were reduced up to 21.88% and 6.84%, respectively. The surface color, firmness and nutrients content were also better maintained. Therefore, this coated corrugated box with the capability to release ClO2 under the trigger of moisture has great potential to be applied as an antimicrobial packaging for fresh fruits and vegetables.

Study on Antibacterial of Chitosan/Hydroxyapatite Doped Magnesium Composite as a Material for Bone Graft Applications

Hydroxyapatite (HAp) is one of the constituent minerals of bone and teeth, that has been widely used for synthesizing bone graft. Due to the limitation on properties of the hydroxyapatite, it is doping with Magnesium (HAp-Mg). The addition of Chitosan (Chi) was expected to improve the antibacterial properties of HAp-Mg. The present research aims to study the influence of Chitosan with 0, 5, 15, and 25 wt% addition on biocompatibility properties of Chi/HAp-Mg composite. HAp-Mg was synthesized using the sol-gel method; meanwhile, Chi/HAp-Mg composite was manufactured by mixing Chitosan in acetic acid, and HAp-Mg was added into the mixture. The synthesized samples were characterized using XRD and SEM. In vitro antibacterial activities of the Chi/HAp-Mg composite were evaluated against Escherichia coli bacteria. Biocompatibility analysis from antibacterial activity showed that composite with the optimal composition on the addition of 15 wt% Chitosan has the best ability in inhibiting the growth of E. coli bacteria.

Effect of Heterogeneous Deacetylation on the Properties of Northern Shrimp Chitin and Chitosan

The effect of alkaline treatment of shrimp chitin on the molecular weight, the degree of deacetylation and degree of crystallinity of the resulting chitosan is studied. The viscosity of chitosan solutions from repeatedly deacetylated chitin is studied. It is shown that repeated treatment of chitin/chitosan with alkali causes the destruction of polysaccharide macromolecules. After four-time deacetylation and one-time deacetylation of chitin/chitosan for four hours, the molecular weight of the polysaccharide decreases by ten times. The maximum degree of chitosan deacetylation under experimental conditions was 92.0 -92.5%. The diffractograms of chitin and chitosan from the Northern shrimp are of the form typical for samples containing an amorphous phase in addition to a crystalline phase. The degree of crystallinity of chitin from Northern shrimp was 40.8%, of chitosan samples after one-, two-, and three-time deacetylation was 62-65%. For a sample of chitosan obtained after four-time deacetylation, recrystallization, and drying in a freeze dryer, the degree of crystallinity is close to the degree of crystallinity of shrimp chitin. The investigated acetic acid chitosan solutions with a concentration of 5% (wt.) and the chitosan molecular weight of 250, 160 and 130 kDa in their rheological properties are liquid-like non-Newtonian systems, their viscosity decreasing with increasing shear stress. After four-time deacetylation of chitin, the viscosity of chitosan solutions practically does not change with increasing shear stress, which apparently can be due to a significant decrease in the molecular weight of chitosan under these conditions.

Effect of different chelating agents on bovine tissue dissolving capacity of sodium hypochlorite

Background: The success of endodontic therapy depends on thorough cleaning of the canal system. This is done by the use of various irrigants together with mechanical cleaning. The most popular irrigant used is sodium hypochlorite (NaOCl) in different concentrations. It has an added advantage in that it is a tissue dissolvent. To improve the removal of the smear layer in the canal, NaOCl is used with chelating agents such as ethylenediaminetetraacetic acid (EDTA), etidronic acid, or chitosan. The aim of this study was to evaluate the effect of different chelating agents when used with NaOCl on tissue dissolving capability. Materials and Methods: Cleaned bovine tissue was cut into equal pieces of 4 mm × 4 mm × 2 mm and divided into 60 samples having a weight of 70 ± 3 mg. Irrigant solutions were divided into four groups. Group 1: 3% NaOCl, Gp 2: 3%NaOCl + 17% EDTA, Group 3: 3% NaOCl + 18% Etidronic acid, and Group 4: 3% NaOCl + 0.2% chitosan in acetic acid. Samples were taken into test tubes and kept in 10 ml solution of each group for 10 min. Later, samples were taken out from solution and washed with distilled water, blotted dry, and weighed again for change of weight. The difference between the initial and final weights was used to calculate the weight loss. Results: There was a significant difference between the sample weight before and after treatment for all the groups. NaOCl (Group 1) showed best percentage difference of weight loss, followed by NaOCl + Etidronic acid (P Conclusion: Etidronic acid has shown better dissolution capacity as compared to EDTA and chitosan group when used with NaOCl.

Fabrication of Complex Polyelectrolyte Membrane of Chitosan-Pectin Crosslinked as Bioadsorbent

Biopolymers are polymers fabricated from natural polymers which are biodegradable, non-toxic and renewable. This research was aimed to synthesize and characterize biopolymer made from chitosan and pectin and was made to be a membrane cross-linked with glutaraldehyde and then characterized. The chitosan-pectin crosslinked glutaraldehyde polyelectrolite complex (PEC) and membrane can be used as a bioadsorbent for metal and cationic dyes. Firstly, PEC membrane chitosan-pectin crosslinked glutaraldehyde was synthesized by dissolving chitosan in acetic acid and pectin in distilled water and added with glutaraldehyde as a crosslinked agent, then heated at 70 °C. The amount of 1M NaOH solution was added for 12 hours and then cleaned with distilled water and dried at room temperature. Subsequently, the membrane was characterized with FTIR spectrophotometer, medium acidity test, and water uptake. The results of the characterization of PEC mebmbrane chitosan-pectin crosslinked glutaraldehyde with FTIR showed the interaction between –NH3+ of chitosan with –COO- group of pectin at a wavelength of 1604.77 cm-1. The medium acidity test showed that the PEC membrane chitosan-pectin crosslinked with glutaraldehyde was stable at pH 3-9 with water absorption of 266.67%, with the composition of chitosan-pectine membrane of 70:30 with 30% glutaraldehyde

Preparation and properties of chitosan–nanodiamond dispersions and composite films

Nanocomposite-forming solutions of chitosan in acetic acid (CS·HOAc) or hydrochloric acid (CS·HCl) and containing nanodiamonds (NDs) were produced without ultrasound treatment, and CS·HCl–ND and CS·HOAc–ND nanocomposite films were prepared by solution casting. The NDs were uniformly distributed in the polymer matrix by stabilization of the dispersion with use of dilute CS·HOAc or CS·HCl solutions before mixing with the concentrated chitosan solutions. CS·HCl–ND and CS·HOAc–ND composite films were then prepared and characterized by the measurement of mechanical properties and by atomic force microscopy methods (topography, phase-contrast, and Kelvin probe force microscopy imaging). The differences in the structures and properties of the films produced on CS·HOAc and CS·HCl are discussed. A stronger interaction was found between phases in the CS·HCl–ND films than in the CS·HOAc–ND films.

Aggregation-Caused Quenching-Type Naphthalimide Fluorophores Grafted and Ionized in a 3D Polymeric Hydrogel Network for Highly Fluorescent and Locally Tunable Emission

Polymer hydrogels with intense yet tunable fluorescence are of great research interest due to their wide potential use in biological imaging, sensing, information storage, etc. However, the conventional fluorophores such as naphthalimide and its derivatives are usually not recommended to prepare highly fluorescent hydrogels because of their aggregation-caused quenching (ACQ) nature and spontaneous tendency to undergo fluorescence self-quenching in quasi-solid-state hydrogel systems. Additionally, local regulation over fluorescent behavior of hydrogels, despite being important, still remains underdeveloped. Herein, we report highly fluorescent polymeric hydrogels based on conventional ACQ-type naphthalimide fluorophores, followed by spatial and temporal control of their fluorescent behavior. The hydrogels were prepared by one-pot radical copolymerization of naphthalimide-containing monomer and acrylamide in chitosan-acetic acid solution. Their intense emission comes from synergetic anchoring and diluting effect of the protonated naphthalimide moieties grafted on polymer chains, which result in the electrostatic repulsion among ACQ luminogens and reduced PET (photoinduced electron transfer) effect from adjacent dimethylamine groups to naphthalimide fluorophores. After being deprotonated in alkaline conditions, both PET and the ACQ effect work again to greatly quench fluorescence, endowing the hydrogels with pH-sensitive emission behavior. These properties encourage us to develop a diffusion-reaction (D-R) method to spatially and temporally control their fluorescent behavior. Based on these results, the ion-transfer-printing-assisted D-R method was further developed to fabricate many high-precision and meaningful fluorescent patterns on hydrogels. These fluorescent patterns are invisible under daylight but become vivid under specific UV light illumination, suggesting their wide potential applications in information security and transmission.

Study on the denitrification and dephosphorization of the aqueous solution by Chitosan/4A Zeolite/Zr based Zeolite

ABSTRACT The ternary hybrid material chitosan/4A Zeolite/Zr based Zeolite was synthesized by using 4A zeolite molecular sieve, Zr-zeolite and chitosan acetic acid solution as starting materials. The prepared composite were characterized by SEM, FT-IR and XRD. The experimental conditions such as the concentration of chitosan acetic acid solution, mass ratio of mass ratio of chitosan acetic acid solution, 4A zeolite molecular sieve and Zr-zeolite functional material, the optimum calcination temperature were optimized. Under this optimum conditions, the denitrification and dephosphorization effect can reach more than 70% when pH value was at 3–9. Dynamics research results showed that the ammonia nitrogen and phosphorus removal progress by chitosan/4A Zeolite/Zr based Zeolite was in accordance could be well described by the pseudo-second-order kinetic model, and the adsorption rate is up to 6.439 g/min. The isothermal adsorption process was in accordance with Freundlich model.

Comparative study of different chitosan solutions to assist the green synthesis of gold nanoparticles under irradiation

Different chitosan aqueous solutions, i.e., conventional chitosan in acetic acid (CTS-HOAc) and non-derivatized water-soluble chitosan (WSCTS) were used as polymer templates for a green synthesis of gold nanoparticles (AuNPs) under gamma irradiation. Because of molecular template effect, the CTS-HOAc and WSCTS exhibited different performance for AuNPs synthesis under various irradiation doses and HAuCl4 precursor concentrations. The AuNPs formation in WSCTS and CTS-HOAc were investigated through physical appearance, numerical color, surface plasmon resonance, particle morphologies and sizes, AuNPs-polymer interaction and crystal structure. Within the irradiation doses range of 0–50 kGy, the particle sizes of the spherical AuNPs formulated in the WSCTS and CTS-HOAc solutions were in the range of 5–25 nm and 5–80 nm, respectively. The particle size and amount of AuNPs can be controlled by balancing synthetic parameters. Compared with AuNPs in CTS-HOAc, the AuNPs in WSCTS exhibit lower toxicity and promote proliferation of human skin fibroblast cells. The different CTS solution systems played an important role for AuNPs formation under irradiation. WSCTS opens a green pathway for synthesis of AuNPs for further extended to appropriate biomedical and cosmeceutical applications.

Integrating 3D-printed PHBV/Calcium sulfate hemihydrate scaffold and chitosan hydrogel for enhanced osteogenic property

We developed the 3D-printed poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/calcium sulfate hemihydrate (PHBV/CaSH) scaffolds by using fused deposition modelling (FDM) technique and then coated the scaffolds with chitosan (CS) acetic acid solution. After drying and neutralization, CS hydrogel was formed on the surface of the scaffolds. The resultant PHBV/CaSH/CS scaffolds could promote the adhesion and proliferation of rat bone marrow stromal cells (rBMSCs) and enhance the osteogenesis of rBMSCs by up-regulating the expression level of osteogenic genes compared to the PHBV and PHBV/CaSH scaffolds. In vivo studies further demonstrated the PHBV/CaSH/CS scaffolds could effectively promote new bone formation. Therefore, integrating 3D-printed PHBV/CaSH scaffold and CS hrydogel represents a novel strategy to promote osteogensis property, showing full potential for bone defects repair.