Accurate Weight Research Articles

POS-634 BODY FAT MASS PLAYS A IMPORTANT ROLE IN OVER- OR UNDERESTIMATION OF BIOIMPEDANCE SPECTROSCOPY-BASEDDRY WEIGHT FOR THE PATIENTS WITH HEMODIALYSIS

Accurate dry weight (DW) estimation is important for hemodialysis patients. Although bioimpedance spectroscopy (BIS) is commonly used to measure DW, the BIS-based DW frequently differs from the clinical DW.

Analysis of Recombinant Adenovirus Vectors by Ion Trap Charge Detection Mass Spectrometry: Accurate Molecular Weight Measurements beyond 150 MDa.

Adenovirus is one of the largest nonenveloped, double-stranded DNA viruses. It is widely used as a gene therapy vector and has recently received a lot of attention as a novel vaccine platform for SARS-CoV-2. Human adenovirus 5 (HAdV5) contains over 2500 protein molecules and has a 36 kbp genome. Adenovirus is well beyond the range of conventional mass spectrometry, and it was unclear how well such a large complex could be desolvated. Here, we report molecular weight (MW) distributions measured for HAdV5 and for 11 recombinant AdV vectors with genomes of varying lengths. The MW distributions were recorded using ion trap charge detection mass spectrometry (CDMS), a single-particle technique where m/z and charge are measured for individual ions. The results show that ions as large as 150 MDa can be effectively desolvated and accurate MW distributions obtained. The MW distribution for HAdV5 contains a narrow peak at 156.1 MDa, assigned to the infectious virus. A smaller peak at 129.6 MDa is attributed to incomplete particles that have not packaged a genome. The ions in the 129.6 MDa peak have a much lower average charge than those in the peak at 156.1 MDa. This is attributed to the empty particles missing some or all of the fibers that decorate the surface of the virion. The MW measured for the mature virus (156.1 MDa) is much larger than that predicted from sequence masses and copy numbers of the constituents (142.5 MDa). Measurements performed for recombinant AdV as a function of genome length show that for every 1 MDa increase in the genome MW, the MW of the mature virus increases by around 2.3 MDa. The additional 1.3 MDa is attributed to core proteins that are copackaged with the DNA. This observation suggests that the discrepancy between the measured and expected MWs for mature HAdV5 is due to an underestimate in the copy numbers of the core proteins.

Comprehensive Study on the Chemical Profile and Anti-Tumor Activity of Secondary Metabolites Produced by Aspergillus niger

Objective: Chemical investigation of the extract of sponge-derived fungus, Aspergillus niger, was performed by liquid chromatography coupled with QExactive mass spectrometry for the first time. Methods: A total number of 444 constituents were detected, 288 of which were identified positively or tentatively by the comprehensive utilization of accurate molecular weight and fragmentation information acquired from quadrupole orbitrap mass spectrometry. The identified compounds were divided into several types, namely, organic acid, alkaloid, saccharide, amino acid and cyclopeptide, terpenoid, polyketone, phenylpropanoid and other types of compounds. Systematic diagnostic ions and featured fragment patterns were summarized for each type, based on which 8 novel compounds belonging to the same type were characterized. Results: This work provided a rapid approach for the research of micro constituents in a complex analyte. Furthermore, the anti-tumor activity of the extract was evaluated on two different cell lines-Bel-7402 and Hela-S3 in vitro. The tumor-inhibitory effect of the Aspergillus niger extract was confirmed and may be mainly derived from its pro-apoptotic action. Moreover, the extract exerted more significant cytotoxicity in Bel-7402 cells than Hela-S3 cells, indicative of its selectivity on specific tumor cells. Conclusion: The evidence suggested that the Aspergillus niger extract may potentially serve as a remedy for the prevention and therapy of hepatic and breast carcinoma.

Chemical analysis of classical prescription Qianghuo Shengshi Standard Decoction by UHPLC-Q Exactive Orbitrap MS

A UHPLC-Q Exactive Orbitrap MS method was used to analyze the chemical constituents of the classical prescription Qianghuo Shengshi Standard Decoction(QHSS). UHPL conditions were as follows: Waters~(TM) UPLC~(TM) HSS T3 C_(18) column(2.1 mm×100 mm, 1.7 μm) and mobile phase of acetonitrile-0.1% formic acid aqueous solution. Mass spectrometry data of QHSS, each herb extract, and negative sample were collected in both positive and negative ion modes. The chemical constituents of QHSS were identified or tentatively identified based on the accurate molecular weight, retention time, MS fragmentation, comparison with reference substances, and literature reports. A total of 141 compounds were identified, including 18 amino acids, oligosaccharides, oligopeptides, and their derivatives, 19 phenolic acids, 44 coumarins, 18 flavonoids and chromones, 13 saponins, 17 phthalides, and 12 other components. This study comprehensively characterized the chemical constituents of QHSS, laying an experimental basis for the in-depth research on the material basis and quality control of QHSS.

Nine-Axis Sensor for Athlete Physical Training Load Characteristics.

In order to understand the characteristic data of athletes' training load, a method based on nine-axis sensor was proposed. Twenty-seven male college athletes were tested twice with a time interval of more than 48 hours. In part 1, participants take the 1 Repetition Maximum (1RM) test. The results show that maximum strength is one of the basic factors to develop the output power of athletes. In the process of skeletal muscle contraction, the curve of speed, force, and power is closely related. When the external load is 10%∼70%, the average power increases with the increase in the average force, it increases with the decrease in the average speed, and at 70%1RM, the average power reaches the peak and then decreases at an inflection point. It is proved that the accurate weight ratio of strength training is the basis of winning athletes, the focus of high level physical coach, and the premise of scientific sports training.

Measuring transit-oriented development in Algerian light rail transit lines by using hybrid multi-criteria decision making and open data sources

Selecting transit-oriented development (TOD) sites along light rail transit lines (LRTLs) is difficult for city planners and decision makers. Selecting the actors and the charges involved in the prioritising process, particularly, when selecting between regions with criteria, is critical. Therefore, this study provided a measuring tool to identify TOD opportunities between regions, and outlined its defining criteria through an easy, rapid, and cost-effective method. In this measuring tool, a multi-criteria decision making approach with a hybrid weighting technique was used for ranking eight criteria representing five principles of TOD. Subsequently, simple additive weighting and technique for order of preference by similarity to ideal solution methods were used to rank LRTLs. Furthermore, open data sources were used for data acquisition, and QGIS software was used for data analysis. The application of this method on six LRTLs of Algeria revealed that three tramlines are highly appropriate for TOD implementation, and the rest of the three LRTLs require interventions to some TOD requirements. The density criterion was confirmed as the critical parameter of TOD measurements around tramlines in Algeria followed by exchange poles and transit ridership. The station number and ticket price were the least critical criteria. The results revealed that integrated weighting is critical for investigating TOD as a new planning approach. This result promoted the assignment of accurate weights, which can be useful for decision makers when working in unexplored fields or during indecisive situations. Weighting criteria can be used as guidelines for the major requirements for locations suitable for TOD. Eventually, these results are valuable for future investments of TOD projects along LRTLs or other transit modes.

Solvent-Independent Molecular Weight Determination of Polymers Based on a Truly Universal Calibration.

Diffusion-ordered NMR spectroscopy (DOSY) allows for accurate molecular weight calibration and determination that can be corrected for solvent influences. Polystyrene and poly(ethylene glycol) standards have been used to calibrate DOSY diffusion data for a variety of solvents, showing a high correlation of data when the bulk viscosity of the solvent is accounted for following the Stokes-Einstein equation. In this way, a type of universal calibration is introduced that allows for determinations of average molecular weight that are at least as accurate as those of traditional size-exclusion chromatography (SEC), if not better. Further, we demonstrate that DOSY calibrations can be used between laboratories, hence removing the need for individual calibration of setups as currently done.

Solvent‐Independent Molecular Weight Determination of Polymers Based on a Truly Universal Calibration

AbstractDiffusion‐ordered NMR spectroscopy (DOSY) allows for accurate molecular weight calibration and determination that can be corrected for solvent influences. Polystyrene and poly(ethylene glycol) standards have been used to calibrate DOSY diffusion data for a variety of solvents, showing a high correlation of data when the bulk viscosity of the solvent is accounted for following the Stokes–Einstein equation. In this way, a type of universal calibration is introduced that allows for determinations of average molecular weight that are at least as accurate as those of traditional size‐exclusion chromatography (SEC), if not better. Further, we demonstrate that DOSY calibrations can be used between laboratories, hence removing the need for individual calibration of setups as currently done.

Embedded microcomputer-based force plate system validation when evaluating lameness severity differentiation under an induced synovitis model in lactating dairy cattle

Bovine lameness has relatively large negative economic and welfare implications on the U.S. dairy industry. Due to the ramifications, early lameness detection will aid in assisting dairy producers to mitigate downstream effects through early treatment. The objective of this study was to determine the minimum standing time required among 2-, 3-, 4-, 5-, and 10 min time intervals to obtain an accurate weight distribution estimate for each leg when attempting to detect lameness. An embedded microcomputer-based force plate system was developed to measure vertical forces from individual cow limb weight distribution to detect bovine lameness when utilizing an induced synovitis lameness model. The force plate has four quadrants, with each load cell quadrant measuring the force placed on it from a single limb. The force plate recorded weight (kg) every second from each load cell quadrant, after which, a 60 s moving average for weight distribution was calculated. A sequential study design was employed to evaluate non-lame and induced lameness to ensure time requirements were consistent. Prior to induction, the force plate system was used to measure weight distribution every second for 15 min. After lameness induction, additional 15 min increments were recorded every 24 h for seven days. Lameness was induced by injecting the left hind distal interphalangeal joint in three cows with amphotericin B, 12 h prior to the start of the study. Data were analyzed using a linear mixed effect that included the fixed effects of day relative to lameness induction, time period, foot and injected foot. Cow within replicate was included as a random effect. Cumulative minutes were assessed up to 15 min by comparing the least square rolling 60 s cumulative means expressed as a percentage of each animal’s BW percentage placed on each leg for 2-, 3-, 4-, 5-, and 10 min intervals. Results indicate that the minimum time needed for accurate lameness detection in cows was 2 min.

Estimation of Hemi-Abdominal-Based Free Flap Weight Using Two Computed Tomography-Derived Measurements.

Accurate flap weight estimation is crucial for preoperative planning in microsurgical breast reconstruction; however, current flap weight estimation methods are time consuming. It was our objective to develop a parsimonious and accurate formula for the estimation of abdominal-based free flap weight. Patients who underwent hemi-abdominal-based free tissue transfer for breast reconstruction at a single institution were retrospectively reviewed. Subcutaneous tissue thicknesses were measured on axial computed tomography angiograms at several predetermined points. Multivariable linear regression was used to generate the parsimonious flap weight estimation model. Split-sample validation was used to for internal validation. A total of 132 patients (196 flaps) were analyzed, with a mean body mass index of 31.2 ± 4.0 kg/m2 (range: 22.6-40.7). The mean intraoperative flap weight was 990 ± 344 g (range: 368-2,808). The full predictive model (R 2 = 0.68) estimated flap weight using the Eq. 91.3x + 36.4y + 6.2z - 1030.0, where x is subcutaneous tissue thickness (cm) 5 cm lateral to midline at the level of the anterior superior iliac spine (ASIS), y is distance (cm) between the skin overlying each ASIS, and z is patient weight (kg). Two-thirds split-sample validation was performed using 131 flaps to build a model and the remaining 65 flaps for validation. Upon validation, we observed a median percent error of 10.2% (interquartile range [IQR]: 4.5-18.5) and a median absolute error of 108.6 g (IQR: 45.9-170.7). We developed and internally validated a simple and accurate formula for the preoperative estimation of hemi-abdominal-based free flap weight for breast reconstruction.

235 THROMBOLYSIS DOSING AND WEIGHT ESTIMATION IN ACUTE STROKE: A SINGLE CENTRE AUDIT

Abstract Background Stroke is a leading cause of death and disability. Thrombolysis with intravenous (IV) alteplase is the mainstay management of ischaemic stroke. It has a narrow therapeutic window with a high potential for adverse outcomes such as intracranial haemorrhage. The efficacy of alteplase is time and dose dependent with weight-based dosing. National clinical guidelines recommend a dose of 0.9 mg/kg, up to a maximum of 90 mg. (Irish Heart Foundation Council for Stroke 2015). In most hospitals in Ireland however, patients are not weighed prior to thrombolysis. Time pressure and lack of available suitable equipment are factors. Methods This retrospective clinical audit evaluated the dosing of alteplase, estimated and actual weight for a convenience sample of stroke thrombolysis patients treated between 2016–2020 at an Irish University Teaching Hospital. Results 107 patients were audited (62 males, 45 females). Actual and estimated weights were available in 92/107. Weight was not documented (n = 15) due to severe stroke/palliative management (n = 6) or omission (n = 9). 21% (19/92) received the correct dose of 0.9 mg/kg. A further 54% (50/92) received a dose within the range of 0.81–0.99 mg/kg (±10%). 25% received a dose outside this range (> ± 10%). 11% (10/92) were under-thrombolysed and 14% (13/92) over-thrombolysed. 17/92 patients had an intracranial haemorrhage. 35% (n = 6/17) of patients who had an intracranial haemorrhage received a higher dose of thrombolysis (>10%). Conclusion A quarter of patients received inappropriate dosing of alteplase that was outside the range of ±10% of 0.9 mg/kg. While stroke thrombolysis must be completed urgently, an accurate weight should be determined to avoid errors in dosing. A process evaluation of stroke thrombolysis would provide information on how best to incorporate an objective means of weight measurement without delaying treatment.

Degree of Accuracy of the BMI Z-Score to Determine Excess Fat Mass Using DXA in Children and Adolescents.

Obesity is caused by fat accumulation. BMI Z-score is used to classify the different degrees of weight status in children and adolescents. However, this parameter does not always express the true percentage of body fat. Our objective was to determine the degree of agreement between the fat mass percentage measured by DXA and the stratification of weight according to BMI Z-score in the pediatric age group. We designed a descriptive cross-sectional study. The patients were classified as underweight/normal weight with Z-scores between −2 and +0.99, overweight from 1 to 1.99, obese from 2 to 2.99, and very obese ≥3. We included 551 patients (47% girls), with a mean age of 11.5 ± 2.8 years (3.7–18 years). Higher BMI Z-scores were associated with a higher percentage of total fat (p < 0.001). However, there were important overlaps between both parameters, such that the BMI Z-score classified patients with the same percentage of total fat mass as having a different nutritional status classification. In conclusion, the stratification of weight status according to BMI Z-score revealed that 46.7% of patients had a fat percentage that did not correspond to their classification. For a more accurate weight assessment in clinical practice, we recommend combining anthropometric indices with diagnostic tools that better correlate with DXA, such as electrical bioimpedance.

Epidemiology of Underweight among Infants in Rural Burkina Faso.

ABSTRACT.Infant undernutrition is thought to contribute to growth failure and mortality. We evaluated the patterns in underweight in a population-based sample of children aged 1–11 months in rural northwestern Burkina Faso. Data were collected during the baseline assessment of a community-randomized trial evaluating mass azithromycin distribution in Nouna District, Burkina Faso. A door-to-door census was undertaken for all households in all communities. Infants aged 1–11 months were weighed for weight-based dosing in the trial and their weights were used to calculate weight-for-age Z-scores (WAZ). Underweight was defined as WAZ ≤ 2. We evaluated the age patterns in WAZ and underweight by demographic, seasonal, and geographic characteristics. Of 7,109 infants, 6,077 had accurate weight and global positioning system (GPS) coordinate data (85.5%). Infants were a median of 6 months old (interquartile range [IQR] 3–8) and 48.4% were female. Mean WAZ was −0.68 (SD 1.6) and 19.0% were underweight. The WAZ decreased with increasing age, and the prevalence of underweight increased from 2.5% among 1-month-olds to 27.6% among 11-month-olds. Underweight was more common among boys than girls (22.1% among boys versus 15.6% among girls). Improved latrine use by the household was associated with increased WAZ, and this effect was stronger in male compared with female infants. Given the large burden of underweight among infants, interventions addressing undernutrition should specifically include infants.

Triacylglycerol and Fatty Acid Compositions of Blackberry, Red Raspberry, Black Raspberry, Blueberry and Cranberry Seed Oils by Ultra-Performance Convergence Chromatography-Quadrupole Time-of-Flight Mass Spectrometry.

The triacylglycerol (TAG) compositions of blackberry, red raspberry, black raspberry, blueberry and cranberry seed oils were examined using ultra-performance convergence chromatography-quadrupole time-of-flight mass spectrometry (UPC2-QTOF MS). A total of 52, 53, 52, 59 and 58 TAGs were detected and tentatively identified from the blackberry, red raspberry, black raspberry, blueberry and cranberry seed oils, respectively, according to their accurate molecular weight in MS1 and fragment ion profiles in MS2. OLL was the most abundant TAG in the blackberry, red raspberry and black raspberry seed oils. Furthermore, the fatty acid compositions of the five berry seed oils were directly determined by gas chromatography coupled with mass spectrometry (GC-MS). In addition, the seed oils had total phenolic contents ranging 13.68–177.06 µmol GAE (gallic acid equivalent)/L oil, and significant scavenging capacities against DPPH, peroxyl, and ABTS+ radicals. These results indicated that the combination of UPC2 and QTOF MS could effectively identify and semi-quantify the TAGs compositions of the berry seed oils with sn-position information for the fatty acids. Understanding the TAGs compositions of these berry seed oils could improve the utilization of these potentially high nutritional value oils for human health.

Body Fat Plays an Important Role in of Bioimpedance Spectroscopy-Based Dry Weight Measurement Error for Patients with Hemodialysis.

Accurate dry weight (DW) estimation is important for hemodialysis patients. Although bioimpedance spectroscopy (BIS) is commonly used to measure DW, the BIS-based DW frequently differs from the clinical DW. We analyzed the characteristics of patients whose BIS-based DWs were over- and underestimated. In this retrospective cohort study, we evaluated 1555 patients undergoing maintenance hemodialysis in Chungnam National University Hospital. The gap (DWCP-BIS) was calculated by comparing the BIS and clinical DWs. We analyzed the clinical characteristics of patients with positive (n = 835) and negative (n = 720) gaps. Compared with other patients, the DWCP-BIS-positive group had higher extracellular water (ECW) level and extracellular/intracellular water index (E/I) and had lower weight, body mass index (BMI), lean tissue index (LTI), fat tissue index (FTI), fat mass (FAT), and adipose tissue mass (ATM). The DWCP-BIS-negative group exhibited elevated BMI, FTI, FAT, and ATM; however, it had lower height, ECW, and E/I. Linear regression analysis revealed that FAT significantly predicted DWCP accuracy. The clinical DW of patients with a low fat mass tended to be underestimated, while the clinical DW of patients with comparatively large fat reserves tended to be overestimated. These characteristics will aid in the reduction of BIS-based DW errors.

A method for determining the weight of objective indoor environment and subjective response based on information theory

Buildings are supposed to create a healthy and comfortable environment for people. Considering subjective response, the problem of multi-objective optimization of building environment control becomes more complicated. The weight method can achieve the unification of optimization goals, but the core challenge is to obtain accurate weights based on a small amount of measured data. Information theory provides a way to quantify the importance of parameters. This paper established a method to calculate the weights of subjective and objective parameters quickly based on the information gain ratio, and took the difference in occupants' feedback under satisfied and unsatisfied conditions into account. Then this method was applied to a case study, two office buildings located in Shanghai, China, in which 120 samples (satisfied 104; unsatisfied 16) in Case 1 and 715 samples (satisfied 537; unsatisfied 178) in Case 2 were collected. The weight calculation time was less than 0.1s, and thus the weight of all parameters can be updated quickly when data is accumulated. Among all the parameters, the thermal satisfaction (with a weight of 0.145 in Case 1 and 0.243 in Case 2) and light satisfaction (with a weight of 0.146 in Case 1 and 0.171 in Case 2) were found to have the greatest impact on overall satisfaction. The weight calculation results under satisfied (0.57 and 0.90 of subjective parameters; 0.43 and 0.10 of objective parameters) and unsatisfied conditions (0.55 and 0.70 of subjective parameters; 0.45 and 0.30 of objective parameters) were found to be different, thus, comprehensive consideration of the difference is of great significance to engineering applications.

Spatial modeling of ground subsidence susceptibility along Al-Shamal train pathway in Saudi Arabia

Abstract Al-Shamal train pathway, which is extended between Saudi Arabia and Jordan, is prone to geo-hazards due to the geological features, proximity to faults, earthquake epicenter, and the human activities along the pathway. The objectives of this study are to shed light on the ground subsidence susceptibility along Al-Shamal train pathway in Qarrayat city in Saudi Arabia and develop a ground subsidence susceptibility model to determine the prone areas to the impacts of ground subsidence to mitigate and avoid the loss of life and property. This study integrated the various data types to map the subsidence susceptibility along Al-Shamal train pathway. Nine ground subsidence causative parameters were selected as subsidence controlling factors in the study area including lithology, land cover/land use, elevation, slope, aspect, annual average rainfall, distance to faults, distance to earthquake epicenter, and distance to streams. The analytical hierarchy process is applied to obtain accurate weight to each criterion through the distribution of online Google form questionnaire to experts in different expertise and get their judgments on the weights of ground subsidence causative parameters in the study area. A subsidence susceptibility index was derived by classifying susceptible maps into five classes, namely, very low, low, moderate, high, and very high using the statistical distribution analysis. The results revealed that the study area is subjected to moderate susceptibility with about 32.56. A total of 29.8 and 11.52% of the study area had very low and low susceptibilities, respectively, and 8.44 and 17.68% had very high and high susceptibilities, respectively. The results were validated using the receiver operating characteristic using previous ground subsidence locations. The area under the curve showed 0.971, which is equivalent to 97.1%. Consequently, the findings of the study are thought to be beneficial to managers and decision makers for future planning, mitigating, and preventing subsidence in the study area.

10.3 PERCEIVED CHALLENGES AND BENEFITS OF DEVELOPING VIRTUAL INTENSIVE TREATMENT PROGRAMS FOR ADOLESCENTS AND YOUNG ADULTS WITH EATING DISORDERS

Shortly following the World Health Organization’s declaration of COVID-19 as a global pandemic in March 2020, leadership within our academic medical center requested that we convert our in-person partial hospitalization programs for children, adolescents, and young adults with eating disorders (EDs) to a virtual format. This presentation describes our experiences with developing 2 virtual intensive treatment programs for EDs, with a focus on perceived challenges and benefits. Clinical staff provided their perspectives working within our institution’s 2 virtual intensive treatment programs for EDs, one of which treats children and adolescents (ages 10-16 years) and the other of which treats young adults (ages 17-25 years). Feedback was obtained from an interdisciplinary team of providers, including psychologists, psychiatrists, adolescent medicine physicians, therapists, and dietitians. Providers identified a number of challenges and benefits related to offering an intensive treatment program for EDs using a virtual format. Challenges included decreased supervision of patients’ eating behaviors during and after meals, complications obtaining accurate weight information, patient fatigue and reduced attention following multiple hours of virtual sessions, patient hesitancy speaking in virtual groups, fewer opportunities for in-session exposure therapy, and difficulties with technology. Benefits included greater access to services for patients with geographical limitations, easier involvement of family members in treatment, greater emphasis on between-session exposure exercises, increased generalizability to patients’ natural environment, reduced disruption of prosocial activities and responsibilities, and more seamless transitions to outpatient treatment. COVID-19 has rapidly accelerated the mental health field’s adoption of telehealth services and forced providers to modify intensive treatment programs in novel ways. Our team experience suggests that there are a number of challenges and benefits to offering virtual treatment, which has implications for the future of telehealth for children, adolescents, and young adults with EDs.

Diradicals or Zwitterions: The Chemical States of m -Benzoquinone and Structural Variation after Storage of Li Ions

Diradicals or Zwitterions: The Chemical States of <i>m</i> -Benzoquinone and Structural Variation after Storage of Li Ions

Body weight perception, knowledge, practices and associated factors regarding weight reduction: A cross sectional study of treatment seeking obese young adults in Sri Lanka

Background: Obesity and its’ comorbidities in young adults are emerging health concerns. Obesity is a well-known risk factor for developing cardiovascular diseases, type -2 diabetes, carcinomas, gallbladder disease, sleep apnea, osteoarthritis, and gynecological complications. Young adulthood is considered the best time period to introduce weight control interventions as the established lifestyle behaviors may persist throughout life.&#x0D; Aims and Objectives: The present study assessed self-perception of body weight, knowledge and practices on obesity and weight reduction and their associated factors in a sample of treatment seeking obese young adults.&#x0D; Materials and Methods: Two-hundred and sixty-one obese (BMI ≥ 25 kg m-2) young (18-35 years) adults attending an out-patient nutrition clinic of a teaching hospital in Sri Lanka were studied. An interviewer administered questionnaire was used to collect demographic data and information on body weight perception and, knowledge and practices on obesity and weight reduction. Weight, height and waist circumference were measured.&#x0D; Results: The mean (±SD) BMI of the participants was 29.0 (±3.6) kg m-2. Only 16.1% precisely perceived themselves as ‘obese’ and increased BMI was only significant predictor of accurate body weight perception (OR=1.23; 95% CI=1.07-1.47; p=0.003). Only 49.0% reported obesity as a disease and 46.0 % and 27.2% that it predisposes to stroke and carcinoma respectively. Although 86.2% have attempted weight reduction, only 33.8% sought professional advice. Diet control was the most common weight reduction strategy (73.1%) and among them 33.3% combined with exercises. One fourth (n=56) relied on green tea for weight reduction and meal skipping was reported in 52.9%.&#x0D; Conclusion: Significant body weight misperception was reported in lower BMI categories. The overall knowledge regarding obesity and weight reduction was satisfactory though not optimum. Diet control with or without physical exercises was the most popular method of weight reduction.