Accumulation Rates Research Articles

Effect of refrigeration and reheating on the lipid oxidation and volatile compounds in silver carp surimi gels.

As unsaturated and saturated aldehydes, ketones are known to be responsible for off-odors in surimi products, and they are mainly derived from lipid oxidation. Because surimi-based products are rich in unsaturated fatty acids, they are prone to producing off-odors during the refrigeration and reheating processes, which are common treatments for leftovers. The present study investigated the color, lipid oxidation productions, fatty acid profiles and volatile components in surimi gels during refrigeration at 4 °C for 3 days with multiple reheating. The results revealed that the accumulation rate of hydroperoxides was higher in the refrigeration stage, whereas the decomposition rate was higher during reheating in surimi gels. Both refrigeration and reheating treatments promoted conjugated diene values, acid values and carbonyl values. Nevertheless, reheating treatment decreased tohiobarbituric acid reactive substances and whiteness. The contents of unsaturated fatty acids, especially α-linolenic acid, arachidonic acid, eicosapentaenoic acid and docosahexaenoic acid, were reduced, whereas the contents of saturated fatty acids increased during refrigeration and multiple reheating. The unsaturated fatty acids were lost as a result of their oxidative deterioration. The volatile components profile showed that the accumulation of volatile components mainly occurred in the refrigeration stage. Multivariate data analysis was utilized to further clarify whether the off-odors in surimi gels were mainly generated in refrigeration. Refrigeration and reheating both contributed to lipid oxidation and the generation of volatile compounds in surimi gels, but the off-odors were mainly generated during refrigeration. This research provides a novel understanding of the formation of food odors in processing. © 2024 Society of Chemical Industry.

Re-organization of Pacific overturning circulation across the Miocene Climate Optimum

The response of the ocean overturning circulation to global warming remains controversial. Here, we integrate a multiproxy record from International Ocean Discovery Program Site U1490 in the western equatorial Pacific with published data from the Pacific, Southern and Indian Oceans to investigate the evolution of deep water circulation during the Miocene Climate Optimum (MCO) and Middle Miocene Climate Transition (MMCT). We find that the northward export of southern-sourced deep waters was closely tied to high-latitude climate and Antarctic ice cover variations. Global warming during the MCO drove a progressive decrease in carbonate ion concentration and density stratification, shifting the overturning from intermediate to deeper waters. In the western equatorial Pacific, carbonate dissolution was compensated by increased pelagic productivity, resulting in overall elevated carbonate accumulation rates after ~16 Ma. Stepwise global cooling and Antarctic glacial expansion during the MMCT promoted a gradual improvement in carbonate preservation and the initiation of a near-modern Pacific overturning circulation. We infer that changes in the latitudinal thermal gradient and in Southern Ocean zonal wind stress and upper ocean stratification drove radically different modes of deep water formation and overturning across the MCO and MMCT.

Dissolved organic matter (DOM) rather than warming and eutrophication directly affects partial pressure of CO2 (pCO2) in mesocosm systems

Environmental warming and eutrophication pose significant challenges to shallow lake systems, where dissolved organic matter (DOM) serves as a diverse and intricate mixture of organic macromolecules, playing a pivotal role in aquatic ecosystems. Despite its complexity, comprehending the interplay between environmental changes and DOM composition alterations and their subsequent impacts on aqueous CO2 partial pressure (pCO2) is essential for a better understanding of carbon cycling. Yet, our current understanding in this realm remains limited. To address this gap, mesocosm systems were established to investigate how elevated water temperature and eutrophication, alongside changes in DOM composition, influence pCO2 dynamics. Results indicate that while temperature and nutrient levels do not directly influence pCO2 fluctuations, they indirectly affect aqueous pCO2 through their modulation of DOM composition. Elevated temperature and nutrient concentrations notably enhance both the production and degradation of indigenous protein-like organic matter and increase the accumulation of humic-like organic compounds, with phosphorus released from sediment playing a particularly significant role. Furthermore, the degradation rate of protein-like organic matter significantly exceeds its accumulation rate. On the other hand, the impact of water eutrophication on DOM composition surpasses that of temporal temperature variations, with a 2∼4 °C temperature rise showing minimal effects on DOM composition. Notably, the degradation of protein-like organic matter markedly increases aqueous pCO2, while the rise in humic-like organic matter in water exerts minimal influence on pCO2 concentrations. A comprehensive understanding of carbon cycling processes under environmental changes will facilitate effective management of lake ecosystems and the advancement of carbon mitigation technologies.

Evaluating the risk of data loss due to particle radiation damage in a DNA data storage system

DNA data storage is a potential alternative to magnetic tape for archival storage purposes, promising substantial gains in information density. Critical to the success of DNA as a storage media is an understanding of the role of environmental factors on the longevity of the stored information. In this paper, we evaluate the effect of exposure to ionizing particle radiation, a cause of data loss in traditional magnetic media, on the longevity of data in DNA data storage pools. We develop a mass action kinetics model to estimate the rate of damage accumulation in DNA strands due to neutron interactions with both nucleotides and residual water molecules, then utilize the model to evaluate the effect several design parameters of a typical DNA data storage scheme have on expected data longevity. Finally, we experimentally validate our model by exposing dried DNA samples to different levels of neutron irradiation and analyzing the resulting error profile. Our results show that particle radiation is not a significant contributor to data loss in DNA data storage pools under typical storage conditions.

Endosperm-specific expressed transcription factor protein WRINKLED1-mediated oil accumulative mechanism in woody oil peony Paeonia ostii var. lishizhenii

Paeonia ostii var. lishizhenii exhibits superiority of high α-linolenic acid in seed oils, yet, the low yield highlights the importance of enhancing oil accumulation in seeds for edible oil production. The transcription factor protein WRINKLED1 (WRI1) plays crucial roles in modulating oil content in higher plants; however, its functional characterization remains elusive in P. ostii var. lishizhenii. Herein, based on a correlation analysis of transcription factor transcript levels, FA accumulation rates, and interaction assay of FA biosynthesis associated proteins, a WRI1 homologous gene (PoWRI1) that potentially regulated oil content in P. ostii var. lishizhenii seeds was screened. The PoWRI1 exhibited an endosperm-specific and development-depended expression pattern, encoding a nuclear-localized protein with transcriptional activation capability. Notably, overexpressing PoWRI1 upregulated certain key genes relevant to glycolysis, FA biosynthesis and desaturation, and improved seed development, oil body formation and oil accumulation in Arabidopsis seeds, resulting an enhancement of total seed oil weight by 9.47–18.77 %. The defective impacts on seed phenotypes were rescued through ectopic induction of PoWRI1 in wri1 mutants. Our findings highlight the pivotal role of PoWRI1 in controlling oil accumulation in P. ostii var. lishizhenii, offering bioengineering strategies to increase seed oil accumulation and enhance its potential for edible oil production.

Rapid achievement of partial nitrification process by adopting the combined strategy of anoxic starvation and free ammonia inhibition

ABSTRACT Partial nitrification (PN) is a prerequisite step for the short-cut nitrogen removal process, which is crucial to provide stable nitrite accumulation for subsequent units. The present study innovatively proposed a new strategy for the rapid establishment of PN by adopting short-term anoxic starvation combined with high free ammonia inhibition. The sludge obtained from the secondary sedimentation tank of a municipal wastewater treatment plant was starved for 7 days under anoxic conditions, and then wastewater with high ammonia nitrogen (400 mg L−1) was introduced. Within 17 days, stable nitrite accumulation was achieved in the sequencing batch reactor, and the nitrite accumulation rate reached more than 95.0%. The activity of ammonia monooxygenase enzyme increased from 0.0364 ± 0.0074 to 0.1275 ± 0.0021 μg NO2 −-N·mg−1 protein min−1, while that of hydroxylamine oxidoreductase enzyme increased from 1.5350 ± 0.0208 to 6.3852 ± 0.0400 EU g−1 SS. The relative abundance of Nitrosomonas increased from 0.10% to 25.90%, while that of Nitrospira consistently remained below 0.04%. And the relative abundance of short-cut denitrifying bacteria, including Truepera, OLB8, and OLB13 all increased. The results proved that the short-term anoxic starvation combined with high free ammonia inhibition was an effective strategy for rapid establishment of PN.

Phytoremediation potential of seedlings: comparing heavy metal accumulation in Ailanthus, Acer, and Fraxinus species.

This study investigates the phytoremediation potential of non-productive seedlings of Ailanthus altissima, Acer pseudoplatanus, and Fraxinus excelsior for lead, cadmium, and zinc accumulation in contaminated soils of Zanjan Province, an industrial area with significant pollution. The evaluation employed a completely randomized design, with three treatment levels for each element, alongside a control treatment, replicated three times over a two-year period. A total of 810 one-year-old seedlings from the three species were involved in the study. Soil contamination levels, ranging from 0 to 2000mg/kg for lead and zinc and from 0 to 200mg/kg for cadmium, were administered through soil pot irrigation. Sampling of seedling stems and pot soils was conducted in November of 2021 and 2022. The absorption levels of elements in the samples were determined using the dry acid digestion method and an ICP-OES atomic absorption spectrometer. Results indicate species-specific variations in metal absorption, with Ailanthus showing the highest accumulation rates. Findings suggest Ailanthus as a promising candidate for soil improvement in polluted environments, particularly in contaminated soils of Zanjan Province.

Heavy Nitrogen Application Rate and Long-Term Duration Decrease the Soil Organic Carbon and Nitrogen Sequestration Rates in Forest Ecosystems

Nitrogen addition alters soil organic carbon (SOC) and total nitrogen (TN) accumulation in forest ecosystems, but the responses of SOC and TN sequestration rates and dynamics to nitrogen addition in forest ecosystems worldwide remain unclear. This study conducted a global analysis to evaluate the effects of the nitrogen application rate, nitrogen addition duration (time), and humidity on the SOC and TN accumulation rates from 257 data points (63 articles). Nitrogen addition increased SOC and TN by 4.48% and 10.18%, respectively. The SOC and TN accumulation rates were 0.65 and 0.11 g kg−1 yr−1, respectively. Moreover, the percentage changes of SOC and TN overall increased with the nitrogen application rate and duration of nitrogen addition; however, the accumulation rates of SOC and TN overall decreased with the nitrogen application rate and the duration of nitrogen addition. In addition, the percentage changes and change rates of SOC and TN increased overall with the humidity index. In conclusion, nitrogen addition promoted SOC and TN accumulation in forest soil, and the nitrogen application rate and nitrogen addition duration increased the percentage changes in SOC and TN; however, they decreased the accumulation rate, whereas humidity increased the accumulation rates of SOC and TN. These results enhance our understanding of soil carbon and nitrogen cycling in forest soils in the context of global nitrogen deposition.

Global Distribution of Mammalian Cradles and Museums is Driven by Past Climate Dynamics and Present Water–Energy Balance

ABSTRACTAimTo describe worldwide distribution of mammalian cradles and museums using the rates of phylogenetic lineage turnover as a surrogate. Additionally, we investigated the influences of current water–energy dynamics, climate instability, past climate changes and elevational ranges on the distribution of these evolutionary zones.LocationGlobal.Time PeriodCurrent.Major Taxa StudiedTerrestrial mammals.MethodsWe developed a new methodology that consists of calculating the spatial phylogenetic turnover for non‐overlapping temporal segments of phylogenetic trees. By calculating the relative turnover in each tree segment, we quantified the rate of accumulation of phylogenetic turnover through time. We depicted cold and hotspots of rates of lineage turnover using bivariate maps and examined the effects of environmental factors using a path model.ResultsThe distributions of cradles and museums of biodiversity are primarily driven by water–energy dynamics. Environments with higher water availability than energetic demand predominantly act as cradles, as seen in tropical rainforests, while xeric‐like environments predominantly serve as museums. Conversely, regions undergoing higher historical climate changes become cradles, such as in higher northern latitudes, while climatically stable areas function as museums. Mountains play a dual role, acting as both cradles and museums by generating new lineages along their elevation bands while simultaneously providing climate refuges for ancient mammal lineages.Main ConclusionsOur findings demonstrate that cradles and museums are not merely a dichotomy but exist along an evolutionary continuum. Furthermore, they reveal how spatial patterns of mammalian cradles and museums are intricately shaped by biogeographical processes governed by environmental forces. Uncovering these hidden effects provides insights into the ecological mechanisms by which ongoing climate changes continually shape evolutionary assemblages over time.

Physiological and Biochemical Effects of Intermittent Warming on 'Marmandi' Turning Tomato Fruits During Low-Temperature Storage

This experiment was conducted on the variety 'Marmandi' turning tomato fruits to investigate the effect of intermittent warming temperatures during extended low chilling temperature storage (21 days) on the subsequent ripening quality of tomato fruits. The quality was measured by assessing the quantitative amount of total phenolic compounds, chlorogenic acid, and ascorbic acid, as well as the development of fruit color and the evaluation of chilling injury (CI) symptoms. The results indicated that the amounts of total phenolic compounds and chlorogenic acid increased significantly during continuous low-temperature storage and after subsequent transfer to room temperature, with this increase being associated with significant loss of ascorbic acid. More frequent periods of intermittent warming (IW) during low-temperature storage were very effective in significantly decreasing the rate of accumulation of phenolic compounds, chlorogenic acid, and ascorbic acid losses. A significant reciprocal relationship between the amounts of chlorogenic acid and ascorbic acid was observed. Additionally, the results showed that as IW time increased, its effectiveness in retarding CI significantly improved.

Anthropogenic and climatic impacts on historic sediment, carbon, and phosphorus accumulation rates using 210Pbex and 137Cs in a sub-watershed linked to Zarivar Lake, Iran

To estimate a watershed’s response to climate change, it is crucial to understand how human activities and climatic extremes have interacted over time. Over the last century, the Zarivar Lake watershed, Iran, has been subjected to various anthropogenic activates, including deforestation and inappropriate land-management practices alongside the implementation of conservation measures like check dams. To understand the effects of these changes on the magnitude of sediment, organic carbon (OC), and phosphorus supplies in a small sub-watershed connected to the lake over the last century, a lake sediment core was dated using 210Pbex and 137Cs as geochronometers. The average mass accumulation rate (MAR), organic carbon accumulation rates (OCAR), and particulate phosphorus accumulation rates (PPAR) of the sediment core were determined to be 6498 ± 2475, 205 ± 85, and 8.9 ± 3.3 g m−2 year−1, respectively. Between the late 1970s and early 1980s, accumulation rates were significantly higher than their averages at 7940 ± 3120, 220 ± 60, and 12.0 ± 2.8 g m−2 year−1 respectively. During this period, the watershed underwent extensive deforestation (12%) on steep slopes, coinciding with higher mean annual precipitations (more than double). Conversely, after 2009, when check dams were installed in the sub-watershed, the sediment load to the lake became negligible. The results of this research indicate that anthropogenic activities had a pronounced effect on MAR, OCAR, and PPAR, causing them to fluctuate from negligible amounts to values twice the averages over the last century, amplified by climatic factors. These results imply that implementing climate-smart watershed management strategies, such as constructing additional check dams and terraces, reinforcing restrictions on deforestation, and minimum tillage practices, can facilitate protection of lacustrine ecosystems under accelerating climate change conditions.Graphical

Assessment of Genetic Diversity in Garden Pea (Pisum sativum L.) and Identification of Promising Lines for Hybridization

The current study is being conducted to understand the degree of genetic divergence in order to discover more diversified parents for pea genetic advancement. The creation of high yielding varieties with stable productivity, resistance to diseases and unfavorable environmental conditions, various maturing types, a high rate of organic matter accumulation during the early stages of growth, a sufficiently high intensity of photosynthesis, increases in protein content, essential amino acids, and favorable ratios among them are the most important tasks for pea breeding. The present investigation was carried out at vegetable research farm and laboratory works in the department of Horticulture (Vegetable and Floriculture), BAU, Sabour, Bhagalpur, Bihar during the Rabi season 2020-21. The genotype Kashi Mukti, Kashi Uday, Kashi Nandini and VM-11 of cluster II exhibited superiority for earliness in first flower, days to 50% flowering and days to first picking and significantly better performance as measured by the lowest cluster mean and a substantial improvement in performance while the genotype Haze-02 of cluster IV exhibited superiority for inter-nodal length , plant height , seeds per pod and total sugars (%) based on maximum cluster mean with significantly better performance. It is anticipated that progenies from such diversified crossings will exhibit a broad range of genetic diversity and more potential for transgressive segregant isolation in the later generations. Therefore, a multiple crossing program using these genotypes may be used to retrieve transgressive segregants.

Personal Consumption and Single Persons: An Update

Abstract Kurt V. Krueger’s seminal study used 2005-2009 Consumer Expenditure Survey (CEX) data to examine various measures of personal consumption rates for single persons. Given changes in survey methods, the impacts of inflation over the following decade, changes in tastes and other factors affecting consumer demand, we re-estimate Krueger’s measures using 2015-2019 survey data. The estimation of estate accumulation rates has expanded as well. Single person expenditures and consumption rates using the 2015-2019 data differ from the earlier results. They also differ from those acquired by deflating 2019 earnings to 2009 values and using the 2005-2009 consumption rates. Experts may find it useful to employ single-person expenditure rates using more recent data.

Long-term effect of phenol, quinoline, and pyridine on nitrite accumulation in the nitrification process: performance, microbial community, metagenomics and molecular docking analysis

Phenol, quinoline, and pyridine, commonly found in industrial wastewater, disrupt the nitrification process, leading to nitrite accumulation. This study explores the potential mechanisms through which these biotoxic organic compounds affect nitrite accumulation, using metagenomic and molecular docking analyses. Despite increasing concentrations of these compounds from 40 to 160 mg/L, ammonia nitrogen removal was not hindered, and stable nitrite accumulation rates exceeding 90 % were maintained. Additionally, these compounds inhibited nitrite-oxidizing bacteria (NOB) and enriched ammonia-oxidizing bacteria (AOB) in situ. As the concentration of these compounds rose, protein (PN) and polysaccharide (PS) concentrations also increased, along with a higher PN/PS ratio. Metagenomic analysis further revealed an increase in hao relative abundance, while microbial community analysis showed increased Nitrosomonas abundance, which contributed to nitrite accumulation stability. Molecular docking indicated that these compounds have lower binding energy with hydroxylamine oxidoreductase (HAO) and nitrate reductase (NAR), theoretically supporting the observed sustained nitrite accumulation.

Optimize the ratio of immobilized gel materials to improve gel performance and oxygen mass transfer rate: Achieve rapid start-up and efficient and stable operation of partial nitration

A gel filler with diatomaceous earth, activated carbon, and CaCO3 compounded with polyvinyl alcohol was prepared and optimized. Rapid start-up and efficient and stable operation of partial nitrification (PN) was achieved by immobilization of conventional activated sludge. The results showed that the proper material proportion synergistically improved the performance and oxygen mass transfer rate of the gel. The continuous flow reactor was operated for 10d to successfully start PN, and the maximum ammonia oxidation rate of this filler was 58.30 mg·(L·h)−1 during the stable operation, and the nitrite accumulation rate was maintained above 90 %. However, due to the greater mass transfer resistance of unoptimized fillers, more dissolved oxygen waste and nitrogen loss were caused, resulting in delayed expression of biological activity. Based on the kinetic characterization of ammonia-oxidizing bacteria (AOB) within the filler and high-throughput sequencing, it was demonstrated that the rapid start-up and efficient and stable operation of the PN system was due to the highly oxygenophilic and community-dominant position of AOB within this filler. This study provides new options and insights at the level of immobilization technology for the realization and efficient and stable operation of PN.

Arctic Alaska deepwater organic carbon burial and environmental changes during the late Albian–early Campanian (103–82 Ma)

The middle Cretaceous greenhouse period experienced profound environmental change including episodes of enhanced global burial of organic carbon marked by carbon isotopic excursions (CIEs). However, the role and response of polar regions like the newly formed, partially enclosed Arctic Ocean Basin during middle Cretaceous carbon burial remains enigmatic. We present the first Arctic deepwater CIE record that characterizes conditions offshore of the Alaska margin north of 75°N paleolatitude. Organic carbon isotopes (δ13Corg) and 103–82 Ma ash zircon U-Pb dates from the distal Hue Shale record multiple Albian–Campanian CIEs during slow ∼3–15 m/Myr sediment accumulation rates. Average total organic carbon (TOC) increased substantially during large 2–3 ‰ CIEs of the ∼101 Ma Albian-Cenomanian boundary event (from 7 to 18 % TOC) and ∼94 Ma Cenomanian-Turonian boundary event (5 to 10 % TOC). Turonian TOC remained elevated (8–13 %) during high global sea levels and temperatures of the Cretaceous Thermal Maximum, followed by an increase from 7 to 11 % TOC during the ∼90 Ma late Turonian event 1.5 ‰ CIE. Average TOC subsequently decreased in the Coniacian–Campanian, but relative maxima occurred during subtle 0.5–1 ‰ CIEs interpreted as the ∼87 Ma late Coniacian event (increase from 4 to 7 % TOC), ∼85 Ma Horseshoe Bay event (3.5 to 4.5 % TOC), and ∼84 Ma Santonian-Campanian boundary event (3.5 to 5 % TOC). Increases in hydrogen index and productivity proxies (P, Ba, Nd) that accompanied each CIE episode with enhanced TOC suggest a strong link between marine productivity and organic carbon burial at short-term CIE timescales. However, long-term (>5–8 Myr) changes in trace metal redox (Mo, Fe, V) and salinity (B/Ga) proxies suggest shifts in prevailing environmental conditions at timescales longer than the CIEs. Late Albian–middle Turonian marine salinity occurred during euxinic (103–98 Ma) and suboxic (98–90 Ma) conditions with deposition interpreted to have occurred within and beneath an oxygen minimum zone, respectively. In contrast, late Turonian–early Campanian (90–82 Ma) freshening and restricted euxinic basin conditions may signal the start of widespread restriction known to characterize the Paleogene Arctic. Overall, these results highlight that middle Cretaceous Arctic deepwater remained a productive marine carbon sink coupled to the global carbon cycle despite evolving Arctic greenhouse conditions.

Assessing the Management of Nitrogen Fertilizer Levels for Yield Values, Photosynthetic Characteristics and Non-Structural Carbohydrates in Rice

The interaction between the amount and frequencies of nitrogen application has always been a hot issue in improving crop yield and reducing environmental pollution. Photosynthesis and non-structural carbohydrates (NSCs) play an important role in the formation of rice yield. However, the research on photosynthetic characteristics and NSCs under nitrogen fertilizer management on rice yield is still insufficient. This work was a two-year field trial in China’s Hunan Province in 2020–2021. To analyze the photosynthetic characteristics and NSCs of the hybrid rice “Zhu Liangyou 819” (ZLY819), the experiment was set up with N application frequencies, specifically P1 (basal-tiller fertilizer at a ratio of 5:5), P2 (basal-tiller-spike fertilizer at a ratio of 4:3:3), and P3 (basal-tiller-spike-grain fertilizer at a ratio of 4:3:2:1). Additionally, three distinct amounts of N applications were utilized: N1 (90 kg ha−1), N2 (150 kg ha−1), and N3 (210 kg ha−1). The findings indicated that under the same N application amount, N2 increased the effective spike by 9.32–17.80% and the number of grains per spike by 12.21–13.28% compared with N1. Under the same N application frequency, P3 had the highest effective number of spikes and number of grains per spike, which were 320.83 × 104 ha−1 and 113.99–119.81, respectively. Under the same N application amount, the SPAD and photosynthetic rate (Pn) of N2 at the heading stage were increased by 5.61–5.68% and 11.73–13.81%, respectively, compared with that of N1; and at the maturity stage, the SPAD of N2 was increased by 14.79–17.21%. At the same N application frequency, SPAD and Pn were 5.40–6.78% and 4.70–12.85% higher in P3 compared to P1, respectively, at the heading stage. At maturity, SPAD showed 14.59–15.64% higher values in P3 compared to P1. The photosynthetically active radiations (PAR) and radiation use efficiency (RUE) of ZLY819 obtained the highest values under N2 or N3 as the differences between these both were nonsignificant. PAR and RUE tended to increase with the increase in the application frequency. NSC accumulation, output, and contribution rate to grains all exhibited a pattern of initial increase followed by a subsequent decline in response to escalating nitrogen application, i.e., it was highest under N2 treatment. A statistically significant positive correlation was observed between rice yield and effective number of spikes, number of grains per spike, SPAD, Pn RUE, output of NSCs, and contribution rate to grains. Appropriate amount and frequency of N application (P3N2) can significantly improve photosynthetic characteristics and NSCs of rice, thus increasing rice yield.

Feasibility and Effectiveness of Venoarterial Extracorporeal Membrane Oxygenation Plus Intra-Aortic Balloon Pump Assisted High-Risk Percutaneous Coronary Intervention in Complex Coronary Disease.

Mechanical circulatory support may facilitate high-risk percutaneous coronary intervention (PCI). This study aimed to assess the feasibility, safety and effectiveness of high-risk PCI under the support of venoarterial extracorporeal membrane oxygenation (VA-ECMO) combined with intra-aortic balloon pump (IABP). We enrolled patients who received VA-ECMO plus IABP-assisted PCI procedures at our center from April 2012 to June 2018. Major adverse cardiac events (MACEs) included all-cause death, myocardial infarction, and target vessel revascularization. A total of 10 patients were included, with a mean age of 71 years, EuroSCORE II of 19.9%, and SYNTAX score of 39.8. Procedural success was achieved in nine (90%) patients. The mean duration of ECMO support was 1.5 hours, and 2.6 stents were implanted per patient. Major complications included contrast-induced nephropathy needing hemodialysis in one (10%) patient, significant hemoglobin drop requiring blood transfusion in two (20%) patients, pulmonary infection in one (10%) patient, and local surgical incision infection in one (10%) patient. The accumulative mortality rates for the nine patients with procedural success were 0, 22.2%, and 44.4% at 1, 3, and 5 years follow-up, respectively. However, cardiac death occurred in only one (11.1%) patient. In addition, two patients received repeat PCI or coronary artery bypass grafting within two years following the index procedure. The overall incidence rates of MACEs were 11.1%, 44.4%, and 66.7% at 1, 3, and 5 years follow-up, respectively. VA-ECMO plus IABP-assisted high-risk PCI was feasible in patients with complex coronary disease, with a high procedural success rate and acceptable mid-term clinical outcomes.

HCC-1 Accelerates Atherosclerosis by Inducing Endothelial Cell and Macrophage Pyroptosis and Serves as an Early Diagnostic Biomarker.

HCC-1 (hemofiltrate CC chemokine-1), a CC-type chemokine, exerts function to change intracellular calcium concentration, induce leukocyte, and manipulate enzyme release especially in monocytes. It has been reported that HCC-1 can predict the persistent acute kidney injury or suppress hepatocellular carcinoma by modulating cell cycle and promoting apoptosis; however, the effect of HCC-1 on atherosclerosis is poorly understood. Here, we aimed to clarify the function and mechanism of HCC-1 in atherosclerosis and whether it could serve as a novel biomarker for the diagnosis of atherosclerosis. HCC-1 expression in serum, atherosclerotic plaques, and normal arterial tissue from patients with atherosclerosis and control group was assessed by ELISA, immunohistochemistry and confocal microscope, and bioinformatic analysis. The atherosclerotic model of HCC-1 overexpressing and control mice was generated by tail vein injection of adeno-associated virus serotype 9-HCC-1 on an ApoE-/- background. Cell adhesion, polarization, and pyroptosis were evaluated in vitro. The relationship between HCC-1 concentration in serum and atherosclerosis was analyzed in patients with atherosclerosis. HCC-1 expression was positively correlated with the occurrence and stable-unstable switch of atherosclerosis under bioinformatic analysis, which is further supported by the results of increased HCC-1 expression in atherosclerosis patients both in serum and atherosclerotic plaque. adeno-associated virus serotype 9-HCC-1 mice had higher levels of inflammatory factors, increased macrophage accumulation and pyroptotic rate in plaque, and decreased atherosclerotic plaque stability. In vitro, HCC-1 promoted monocyte adhesion and M1 polarization and induced inflammation and pyroptosis both in endothelial cells and macrophages. HCC-1 expression was increased in patients with atherosclerosis, and HCC-1 overexpression accelerated atherosclerotic burden via an enhancement in monocyte recruitment, M1 polarization, and pyroptosis both in endothelial cells and macrophages. Our findings suggested that HCC-1 may serve as an early biomarker for the diagnosis of atherosclerosis, with the capacity to reflect the degree of stenosis.

Reconstructing the Zama (Mexico) discovery source to sink story, Part I; detrital zircon U–Pb and (U‐Th)/He provenance analysis and implications for sediment source terranes

AbstractThe Zama discovery was identified off the coast of Tabasco, Mexico, in the Sureste Basin of the Gulf of Mexico and is hosted in a three‐way closure in the Upper Miocene. This study conducted a detailed detrital zircon (DZ) U–Pb and (U‐Th)/He provenance analyses on samples from sandstone reservoirs in the Zama‐3 and Zama‐2ST1 wells. A total of 22 sandstone samples (11 from each well) were collected for DZ U–Pb and (U‐Th)/He dating from different reservoir zones, testing the hypothesis that different zones were whether originally derived from varied sedimentary source terranes and associated transport pathways to the Zama mini‐basin depositional site. Additional objectives include determination of maximum depositional ages, reconstruction of paleofluvial systems, and exploring the temporal evolution of the drainage region and hinterland tectonics. The DZ U–Pb age spectra from both Zama wells have remarkably homogenous DZ signatures with very similar DZ age modes and modal percentages, displaying dominant Permian/Chiapas Batholith (ca. 35%–45%), Mesoproterozoic/Oaxaquian (ca. 20%–35%), Early Palaeozoic/Acatlán (ca. 8%–20%), and Cenozoic magmatic arc (ca. 15%–25%) age modes, as well as some subsidiary (<5%) early Proterozoic/Archean and Early Cretaceous DZ age components, linked to recycled lower Palaeozoic strata and the Guerrero Terrane and Alisitos arc, respectively. Despite differences in paleocurrent directions, deduced from image logs, there are no systematic differences in DZ spectra, indicating a consistent sediment provenance and no changes in source area. All Zama samples analysed in the study are characterized by abundant syn‐depositional Late Miocene DZ grains, clustering between 8.6 and 10.2 Ma, corroborating a Tortonian (Late Miocene) depositional age, and yield rapid sediment accumulation rates of ca. 200 m in <1.4 Ma (13 m/Myr). Doubled zircon U–Pb and (U‐Th)/He age pairs are indicative of recycling of early Mesozoic rift strata and Paleogene and younger Chiapas basement. These new DZ U–Pb and (U‐Th)/He data have a nearly invariant Tortonian sediment provenance that is similar to the modern Grijalva River flowing generally northward out of the Chiapas highlands. The paleo‐Grijalva drainage, providing sediment to the Late Miocene Zama mini‐basin, was likely drastically larger than the present catchment as it involved 10 Ma plutonic sources that were subsequently downfaulted along the Pacific coast in the latest Miocene. Importantly, DZ U–Pb age components are consistent with Oaxaquia, early Acatlán, and Guerrero/Alisitos signatures and point to sourcing from the Chortis block during its tectonic eastward translation. Such a scenario would allow for a substantially larger Miocene paleo‐drainage that would have encompassed both Chiapas and portions of Chortis. The Miocene tectonic translation of Chortis and erosion of a large and tectonically active hinterland would also reconcile the dramatically larger Middle to Late Miocene sediment supply, funnelling a large influx of sediment into the southern Gulf of Mexico. Rapid Late Miocene sediment accumulation in the salt‐defined Zama mini‐basin must have involved sustained sediment flux via the paleo‐Grijalva drainage and was likely facilitated and focused by continued salt deflation due to sediment loading, as described in Part II. Thus, our work provides new scientific insights into one of the largest hydrocarbon discoveries in Mexico in recent years.