In the present investigation, the bioaccumulation of toxic metals (Pb, Cd, Cr) concentration and histological evaluation in heart and kidney tissues of were estimated in Devario aequipinnatus, which were caught from the four sampling stations of river Tamiraparani, Tamilnadu, India. The results revealed that Pb, Cd and Cd concentrations in Devario aequipinnatus tissues were considerably lower than at river Tamiraparani sites. Pb concentration was greatest in the kidney (0.16 μg/g dry wt.), Cr content was virtually same in the kidney (0.14 μg/g dry wt.) and in the heart (0.13 μg/g dry wt.), and Cr content was mostly enriched in the kidney (0.14 μg/g dry wt.). However, the results indicated significant variations in the three examined metal levels between the species, as well as unique histological changes in D. aequipinnatus heart and kidney tissues. Despite this, toxic metal accumulation with histological analysis indicated the most substantial differences in the Tirunelveli area. Furthermore, it appears that the vast majority of them are extremely harmful rather than favorable for fish species. This study found that fish products can accumulate metals and carry them on to humans, mostly through food, resulting in chronic or acute illnesses.