Accumulation Of Polymers Research Articles

De Novo Assembly of the Polyhydroxybutyrate (PHB) Producer Azohydromonas lata Strain H1 Genome and Genomic Analysis of PHB Production Machinery

Polyhydroxybutyrate (PHB) is a biodegradable natural polymer produced by different prokaryotes as a valuable carbon and energy storage compound. Its biosynthesis pathway requires the sole expression of the phaCAB operon, although auxiliary genes play a role in controlling polymer accumulation, degradation, granule formation and stabilization. Due to its biodegradability, PHB is currently regarded as a promising alternative to synthetic plastics for industrial/biotechnological applications. Azohydromonas lata strain H1 has been reported to accumulate PHB by using simple, inexpensive carbon sources. Here, we present the first de novo genome assembly of the A. lata strain H1. The genome assembly is over 7.7 Mb in size, including a circular megaplasmid of approximately 456 Kbp. In addition to the phaCAB operon, single genes ascribable to PhaC and PhaA functions and auxiliary genes were also detected. A comparative genomic analysis of the available genomes of the genus Azohydromonas revealed the presence of phaCAB and auxiliary genes in all Azohydromonas species investigated, suggesting that the PHB production is a common feature of the genus. Based on sequence identity, we also suggest A. australica as the closest species to which the phaCAB operon of the strain H1, reported in 1998, is similar.

A Study on the Development of Real-Time Chamber Contamination Diagnosis Sensors.

Plasma processes are critical for achieving precise device fabrication in semiconductor manufacturing. However, polymer accumulation during processes like plasma etching can cause chamber contamination, adversely affecting plasma characteristics and process stability. This study focused on developing a real-time sensor system for diagnosing chamber contamination by quantitatively monitoring polymer accumulation. A quartz crystal sensor integrated with flexible printed circuit boards was designed to measure the frequency shifts corresponding to polymer thickness changes. An impedance probe was also employed to monitor variations in the plasma discharge characteristics. The sensor demonstrated high reliability with a measurement scatter of 2.5% despite repeated plasma exposure. The experimental results revealed that polymer accumulation significantly influenced the plasma impedance, and this correlation was validated through real-time monitoring and scanning electron microscopy (SEM). The study further showed that the sensor could detect the transition point of the plasma state changes under varying process gas conditions, enabling the early detection of potential process anomalies. These findings suggest that the developed sensor system can be crucial for diagnosing plasma and chamber conditions, providing valuable data for optimizing preventive maintenance schedules. This advancement offers a pathway for improving process reliability and extending the operational lifetime of semiconductor manufacturing equipment.

Enhanced biosynthesis of poly(3-hydroxybutyrate) in engineered strains of Pseudomonas putida via increased malonyl-CoA availability.

Malonyl-coenzyme A (CoA) is a key precursor for the biosynthesis of multiple value-added compounds by microbial cell factories, including polyketides, carboxylic acids, biofuels, and polyhydroxyalkanoates. Owing to its role as a metabolic hub, malonyl-CoA availability is limited by competition in several essential metabolic pathways. To address this limitation, we modified a genome-reduced Pseudomonas putida strain to increase acetyl-CoA carboxylation while limiting malonyl-CoA utilization. Genes involved in sugar catabolism and its regulation, the tricarboxylic acid (TCA) cycle, and fatty acid biosynthesis were knocked-out in specific combinations towards increasing the malonyl-CoA pool. An enzyme-coupled biosensor, based on the rppA gene, was employed to monitor malonyl-CoA levels invivo. RppA is a type III polyketide synthase that converts malonyl-CoA into flaviolin, a red-colored polyketide. We isolated strains displaying enhanced malonyl-CoA availability via a colorimetric screening method based on the RppA-dependent red pigmentation; direct flaviolin quantification identified four engineered strains had a significant increase in malonyl-CoA levels. We further modified these strains by adding a non-canonical pathway that uses malonyl-CoA as precursor for poly(3-hydroxybutyrate) biosynthesis. These manipulations led to increased polymer accumulation in the fully engineered strains, validating our general strategy to boost the output of malonyl-CoA-dependent pathways in P. putida.

Fibrosis, biomarkers and liver biopsy in AAT deficiency and relation to liver Z protein polymer accumulation.

The course of adults with ZZ alpha-1-antitrypsin deficiency (AATD) liver disease is unpredictable. The utility of markers, including liver biopsy, is undefined. A prospective cohort, including protocol liver biopsies, was enrolled to address these questions. We enrolled 96 homozygous ZZ AATD adults prospectively at three US sites with standardized clinical evaluations, and protocol liver biopsies. Fibrosis was scored using Ishak (stages 0-6). Also, 51% of the 96 subjects had Ishak score >1 fibrosis (49% Ishak 0-1, 36% Ishak 2-3 and 15% ≥4). Elevated aspartate aminotransferase (AST) more than alanine aminotransferase (ALT), high body mass index (BMI), obesity, AST platelet ratio index and elevated serum Z alpha 1 antitrypsin (AAT) polymer levels were associated with increased fibrosis. Steatosis did not correlate to fibrosis. Increased fibrosis was associated with increased mutant Z polymer globular inclusions (p = .002) and increased diffuse cytoplasmic Z polymer on biopsy (p = .0029) in a direct relationship. Increased globule Z polymer was associated with increased serum AST (p = .007) and increased periportal inflammation on histopathology (p = .004), but there was no relationship of Z polymer hepatocellular accumulation with ALT, gamma glutamine transferase, inflammation in other parts of the lobule, necrosis or steatosis. Serum Z polymer levels were directly correlated to hepatic Z protein polymer content. Lung function, smoking and alcohol consumption patterns were not associated with fibrosis. In AATD high BMI, obesity and elevated AST are associated with increased fibrosis. Liver biopsy features are correlated to some serum tests. Serum Z AAT polymer levels could be a future biomarker to detect fibrosis early and is directly correlated to liver Z content.

Microbial production of an aromatic homopolyester

We report the development of a metabolically engineered bacterium for the fermentative production of polyesters containing aromatic side chains, serving as sustainable alternatives to petroleum-based plastics. A metabolic pathway was constructed in an Escherichia coli strain to produce poly[d-phenyllactate(PhLA)], followed by three strategies to enhance polymer production. First, polyhydroxyalkanoate (PHA) granule-associated proteins (phasins) were introduced to increase the polymer accumulation. Next, metabolic engineering was performed to redirect the metabolic flux toward PhLA. Furthermore, PHA synthase was engineered based on in silico simulation results to enhance the polymerization of PhLA. The final strain was capable of producing 12.3 g/l of poly(PhLA), marking it the first bio-based process for producing an aromatic homopolyester. Additional heterologous gene introductions led to the high level production of poly(3-hydroxybutyrate-co-11.7 mol% PhLA) copolymer (61.4 g/l). The strategies described here will be useful for the bio-based production of aromatic polyesters from renewable resources.

Advances in Microbial Biotechnology for Sustainable Alternatives to Petroleum-Based Plastics: A Comprehensive Review of Polyhydroxyalkanoate Production.

The industrial production of polyhydroxyalkanoates (PHAs) faces several limitations that hinder their competitiveness against traditional plastics, mainly due to high production costs and complex recovery processes. Innovations in microbial biotechnology offer promising solutions to overcome these challenges. The modification of the biosynthetic pathways is one of the main tactics; allowing for direct carbon flux toward PHA formation, increasing polymer accumulation and improving polymer properties. Additionally, techniques have been implemented to expand the range of renewable substrates used in PHA production. These feedstocks are inexpensive and plentiful but require costly and energy-intensive pretreatment. By removing the need for pretreatment and enabling the direct use of these raw materials, microbial biotechnology aims to reduce production costs. Furthermore, improving downstream processes to facilitate the separation of biomass from culture broth and the recovery of PHAs is critical. Genetic modifications that alter cell morphology and allow PHA secretion directly into the culture medium simplify the extraction and purification process, significantly reducing operating costs. These advances in microbial biotechnology not only enhance the efficient and sustainable production of PHAs, but also position these biopolymers as a viable and competitive alternative to petroleum-based plastics, contributing to a circular economy and reducing the dependence on fossil resources.

Autotrophic poly-3-hydroxybutyrate accumulation in Cupriavidus necator for sustainable bioplastic production triggered by nutrient starvation

Cupriavidus necator is a facultative chemolithoautotrophic bacterium able to convert carbon dioxide into poly-3-hydroxybutyrate. This is highly promising as the conversion process allows the production of sustainable and biodegradable plastics. Poly-3-hydroxybutyrate accumulation is known to be induced by nutrient starvation, but information regarding the optimal stress conditions controlling the process is still heterogeneous and fragmentary. This study presents a comprehensive comparison of the effects of nutrient stress conditions, namely nitrogen, hydrogen, phosphorus, oxygen, and magnesium deprivation, on poly-3-hydroxybutyrate accumulation in C. necator DSM545. Nitrogen starvation exhibited the highest poly-3-hydroxybutyrate accumulation, achieving 54% of total cell dry weight after four days of nutrient stress, and a carbon conversion efficiency of 85%. The gas consumption patterns indicated flexible physiological mechanisms underlying polymer accumulation and depolymerization. These findings provide insights into strategies for efficient carbon conversion into bioplastics, and highlight the key role of C. necator for future industrial-scale applications.

A novel electric field-assisted material extrusion process for clean additive manufacturing

Material extrusion (MEX) is a type of additive manufacturing technique that is widely used in a variety of applications. However, the issue of reduced quality of printed parts caused by degraded polymer debris accumulation on the nozzle surface during the MEX process has received very limited attention. This work presents a novel electric field-assisted MEX (E-MEX) method to solve the quality issue by significantly reducing the accumulations of polymer debris on the nozzle. By applying a high-voltage power source between the nozzle and the build plate, an electrostatic force was generated to overcome the which showed great potential to reduce the accumulation of molten thermoplastic material on the nozzles. Both the E-MEX and conventional MEX methods were used to print test specimens for specific amounts of time to confirm the efficacy of the E-MEX method. 3D scanning was used to quantify the volumes of debris that had accumulated on the nozzle. Compared to the conventional MEX method, the E-MEX printing reduced debris accumulation by 47 % after about 5 h and over 80 % after about 15 h of the printing process. The changes in electric field intensity over orienting height were analyzed using numerical simulations. The relationship between electric field intensity and time spent by the nozzle during the printing process at a specific height was established. The polymer accumulation in the E-MEX process was found to be dependent on the electric field intensity and the continuous time spent by the nozzle in the weaker electric field enhanced polymer debris accumulations on the nozzle surfaces. The presented study integrates experimental observations with simulation results, revealing the mechanism of debris accumulation on the nozzle when the electric field decreased to a certain level during printing process. A numerical simulation model was developed to understand the traveling of accumulated polymer debris from the nozzle's outer surface to the printing layer under the influence of electrostatic force. The simulation results showed that the dragging of polymer debris towards the build plate occurs when the electric force acting in the tangential and normal direction is sufficient to overcome the viscous force and normal force in the polymer debris. The simulation results also showed some part of the debris remained in the crease area of the nozzle surface due to the viscous and surface tension forces prevailing over the electric force.

Metabolic Reprogramming of Human Macrophages by Sickle Cell Hemoglobin

BACKGROUND: Sickle cell disease is caused by a single nucleotide mutation in the β-globin gene, resulting in the substitution of valine for glutamic acid, polymerization of hemoglobin and sickling of red blood cells (RBCs) under low oxygen conditions causing chronic hemolysis, and organs damage. SCA is characterized by the presence of cell-free plasma hemoglobin. Organ damage is associated with chronic inflammation and pro-inflammatory macrophages phenotype. Typically, anti-inflammatory response is elicited by the phagocytosis of senescent RBCs and cell-free hemoglobin. We have demonstrated that treatment of human macrophages with sickle cell hemoglobin (HbS) leads to lysosome accumulation, autophagy activation, and pro-inflammatory phenotype. The propensity of mutant HbS to polymerize under low pH may promote lysosomal HbS polymer accumulation, hindering degradation, and inducing macrophage differentiation towards a pro-inflammatory state. The differentiation of macrophages into specific phenotypes is associated with significant metabolic reprogramming. HYPOTHESIS: We hypothesize that the uptake of HbS by human macrophages induces their metabolic reprogramming via mitochondrial remodeling. METHODS: Human THP-1 cells were differentiated into macrophages with PMA and treated with either purified HbS or normal hemoglobin (HbA). Mitochondria were detected with MitoTrackerTM Green FM dye, and mitochondrial ROS (mtROS) level was detected by Mito SOX Red Kit. Transmission electron microscopy imaging was performed at Talos 200X transmission electron microscope. EPView (Olympus) software was used for the structure analysis. Total RNA was isolated by Trizol and sequenced on an Illumina® NextSeq 500. Comparisons between treatments were conducted using Gene Set Enrichment Analysis (GSEA). RESULTS: Immunofluorescent staining demonstrated accumulation of mitochondria after treatment with HbS compared to untreated (ctrl) or HbA-treated macrophages. Electron microscopy revealed an increase in the number and size of mitochondria. In 25 random microphotographs of cytoplasm, we detected 51 mitochondria in control, 79 in HbA treated and, 96 in HbS treated macrophages. The mitochondria volume and cristae size were significantly increased in macrophages treated with HbS. There was no increase in mtROS production in HbS treatment compared to ctrl. As expected, HbA treatment reduced mtROS production. The mitochondrial gene set (242 genes) was the second most enriched GSEA set in HbS-treated compared to HbA-treated macrophages. The larger mitochondrial size may be associated with a reduction of fission or an increase in fusion. We did not find differences in the expression of mitochondrial fission and fusion genes between HbS and HbA-treated macrophages. The increase in the mitochondrial size and cristae size may also be associated with increased oxidative phosphorylation. We found significant enrichment of genes responsible for oxidative phosphorylation in the HbS-treated macrophages. We did not find significant differences in the expression of glycolysis genes. CONCLUSION: Treatment of human macrophages with HbS significantly increases the number and volume of mitochondria, size of the cristae, and oxidative phosphorylation without upregulation of mtROS production and reduction of glycolysis. This work was supported by NIH Research Grants SC1HL150685, P50HL118006, and R01HL125005. We thank Drs. Castle Raley and Keith Crandall, George Washington University SPH Genomics Core, for sequencing and guidance in data analysis; Dr. Christine Brantner, GW Nanofabrication and Imaging Center, for TEM imaging. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Modified fructan accumulation through overexpression of wheat fructan biosynthesis pathway fusion genes Ta1SST:Ta6SFT

BackgroundFructans are water-soluble carbohydrates that accumulate in wheat and are thought to contribute to a pool of stored carbon reserves used in grain filling and tolerance to abiotic stress.ResultsIn this study, transgenic wheat plants were engineered to overexpress a fusion of two fructan biosynthesis pathway genes, wheat sucrose: sucrose 1-fructosyltransferase (Ta1SST) and wheat sucrose: fructan 6-fructosyltransferase (Ta6SFT), regulated by a wheat ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (TaRbcS) gene promoter. We have shown that T4 generation transgene-homozygous single-copy events accumulated more fructan polymers in leaf, stem and grain when compared in the same tissues from transgene null lines. Under water-deficit (WD) conditions, transgenic wheat plants showed an increased accumulation of fructan polymers with a high degree of polymerisation (DP) when compared to non-transgenic plants. In wheat grain of a transgenic event, increased deposition of particular fructan polymers such as, DP4 was observed.ConclusionsThis study demonstrated that the tissue-regulated expression of a gene fusion between Ta1SST and Ta6SFT resulted in modified fructan accumulation in transgenic wheat plants and was influenced by water-deficit stress conditions.

Folic Acid-Conjugated Brush Polymers Show Enhanced Blood-Brain Barrier Crossing in Static and Dynamic In Vitro Models Toward Brain Cancer Targeting Therapy.

Over the past decades, evidence has consistently shown that treatment of central nervous system (CNS)-related disorders, including Alzheimer's disease, Parkinson's disease, stroke, multiple sclerosis, and brain cancer, is limited due to the presence of the blood-brain barrier (BBB). To assist with the development of new therapeutics, it is crucial to engineer a drug delivery system that can cross the BBB efficiently and reach target cells within the brain. In this study, we present a potentially efficient strategy for targeted brain delivery through utilization of folic acid (FA)-conjugated brush polymers, that specifically target the reduced folate carrier (RFC, SLC19A1) expressed on brain endothelial cells. Here, azide (N3)-decorated brush polymers were prepared in a straightforward manner coupling a heterotelechelic α-NH2, ω-N3-poly(2-ethyl-2-oxazoline) (NH2-PEtOx-N3) to N-acylated poly(amino ester) (NPAE)-based brushes. Strain-promoted azide-alkyne cycloaddition (SPAAC) 'click chemistry' with DBCO-folic acid (FA) yielded FA-brush polymers. Interestingly, while azide functionalization of the brush polymers dramatically reduced their association to brain microvascular endothelial cells (hCMEC/D3), the introduction of FA to azide led to a substantial accumulation of the brush polymers in hCMEC/D3 cells. The ability of the polymeric brush polymers to traverse the BBB was quantitatively assessed using different in vitro BBB models including static Transwell and microfluidic platforms. FA-brush polymers showed efficient transport across hCMEC/D3 cells in a manner dependent on FA composition, whereas nonfunctionalized brush polymers exhibited limited trafficking under the same conditions. Further, cellular uptake inhibition studies suggested that the interaction and transport pathway of FA-brush polymers across BBB relies on the RFC-mediated pathways. The potential application of the developed FA-brush polymers in brain cancer delivery was also investigated in a microfluidic model of BBB-glioblastoma. Brush polymers with more FA units successfully presented an enhanced accumulation into U-87 MG glioma cells following its BBB crossing, compared to controls. These results demonstrate that FA-modified brush polymers hold a great potential for more efficient delivery of future brain therapeutics.

Microplastic accumulation and ecological impacts on benthic invertebrates: Insights from a microcosm experiment

Microplastic (MP) pollution poses a global concern, especially for benthic invertebrates. This one-month study investigated the accumulation of small MP polymers (polypropylene and polyester resin, 3–500 μm, 250 μg L−1) in benthic invertebrates and on one alga species. Results revealed species-specific preferences for MP size and type, driven by ingestion, adhesion, or avoidance behaviours. Polyester resin accumulated in Mytilus galloprovincialis, Chamelea gallina, Hexaplex trunculus, and Paranemonia cinerea, while polypropylene accumulated on Ulva rigida. Over time, MP accumulation decreased in count but not size, averaging 6.2 ± 5.0 particles per individual after a month. MP were mainly found inside of the organisms, especially in the gut, gills, and gonads and externally adherent MP ranged from 11 to 35 % of the total. Biochemical energy assessments after two weeks of MP exposure indicated energy gains for water column species but energy loss for sediment-associated species, highlighting the susceptibility of infaunal benthic communities to MP contamination.

Amino-Acid-Derived Anionic Polyacrylamides with Tailored Hydrophobicity-Physicochemical Properties and Cellular Interactions.

Polyanions can internalize into cells via endocytosis without any cell disruption and are therefore interesting materials for biomedical applications. In this study, amino-acid-derived polyanions with different alkyl side-chains are synthesized via postpolymerization modification of poly(pentafluorophenyl acrylate), which is synthesized via reversible addition-fragmentation chain-transfer (RAFT) polymerization, to obtain polyanions with tailored hydrophobicity and alkyl branching. The success of the reaction is verified by size-exclusion chromatography, NMR spectroscopy, and infrared spectroscopy. The hydrophobicity, surface charge, and pH dependence are investigated in detail by titrations, high-performance liquid chromatography, and partition coefficient measurements. Remarkably, the determined pK a-values for all synthesized polyanions are very similar to those of poly(acrylic acid) (pK a = 4.5), despite detectable differences in hydrophobicity. Interactions between amino-acid-derived polyanions with L929 fibroblasts reveal very slow cell association as well as accumulation of polymers in the cell membrane. Notably, the more hydrophobic amino-acid-derived polyanions show higher cell association. Our results emphasize the importance of macromolecular engineering toward ideal charge and hydrophobicity for polymer association with cell membranes and internalization. This study further highlights the potential of amino-acid-derived polymers and the diversity they provide for tailoring properties toward drug delivery applications.

Demonstrating scale-up of a novel water treatment process using super-bridging agents

Fiber-based materials have emerged as a promising option to increase the efficiency of water treatment plants while reducing their environmental impacts, notably by reducing the use of unsustainable chemicals and the size of the settling tank. Cellulose fiber-based super-bridging agents are sustainable, reusable, and versatile materials that considerably improve floc separation in conventional settling tanks or via alternative screening separation methods. In this study, the effectiveness of fiber-based materials for wastewater treatment was evaluated at lab-scale (0.25 L) and at pilot-scale (20 L) for two separation methods, namely settling and screening. For the fiber-based method, the performance of floc separation during settling was slightly affected by an 80x upscaling factor. A small decrease in turbidity removal from 93 and 86 % was observed for the jar and pilot tests, respectively. By contrast, the turbidity removal of the conventional treatment, i.e., no fibers with a settling separation, was largely affected by the upscaling with turbidity removals of 84 and 49 % for jar and pilot tests, respectively. Therefore, results are suggesting that fiber-based super-bridging agents could be implemented in full-scale water treatment plants. Moreover, the tested fibers increase the robustness of treatment by providing better floc removal than conventional treatment under several challenging conditions such as low settling time and screening with coarse screen mesh size. Furthermore, at both lab-scale and pilot-scale, the use of fiber-based materials reduced the demand for coagulant and flocculant, potentially lowering the operational costs of water treatment plants and reducing the accumulation of metal-based coagulants and synthetic polymers in sludge. Acute toxicity tests using the model organism Daphnia magna show that the cellulose fibers introduce insignificant toxicity at the optimized fiber concentration. Although dedicated mechanistic studies are required at various scales to understand in detail the influence of fibers on water treatment (coagulation/flocculation time, floc formation, floc size distribution velocity gradient, etc.), the efficacy and scalability of the fiber-based approach, along with its minimal environmental impact, position it as a viable and sustainable option for existing and future wastewater treatment plants.

Adsorption characteristics and mechanisms of water-soluble polymers (PVP and PEG) on kaolin and montmorillonite minerals

The excessive use and accumulation of water-soluble polymers (WSPs, known as “liquid plastics”) in the environment can pose potential risks to both ecosystems and human health, but the environmental fate of WSPs remains unclear. Here, the adsorption behavior of WSPs with different molecular weight on kaolinite (Kaol) and montmorillonite (Mt) were examined. The results showed that the adsorption of PEG and PVP on minerals were controlled by hydrogen bond and van der Waals force. The Fourier transform infrared (FTIR) spectra and two-dimensional correlation spectroscopy (2D-COS) analysis revealed that there were interactions between the Al-O and Si-O groups of the minerals and the polar O- or N-containing functional groups as well as the alkyl groups of PEG and PVP. The adsorption characteristics of WSPs were closely related to their molecular weight and the pore size of minerals. Due to the relatively large mesopore size of Kaol, both PEG and PVP were absorbed into inner spaces, for which the adsorption capacity increased with molecular weight of the polymers. For Mt, all types of PEG could enter its micropores, while PVP with larger molecular weights appeared to be confined externally, leading to a decrease in the adsorption capacity of PVP with increasing molecular weight. The findings of this study provide a theoretical basis for scientific evaluation of environmental processes of WSPs.

Surface chemical reactions of etch stop prevention in plasma-enhanced atomic layer etching of silicon nitride

Molecular dynamics (MD) simulations were performed to examine the influence of oxygen (O) ion irradiation at the end of each cycle of plasma-enhanced atomic layer etching (PE-ALE) of silicon nitride (SiN). The main concern during the SiN PE-ALE process is that it can lead to an etch stop due to excessive hydrofluorocarbon (HFC) polymer accumulation, which hinders ion impact in the desorption step. Such an etch stop can be avoided by incorporating an oxygen (O2) plasma irradiation step after the desorption step of a conventional two-step PE-ALE process of SiN. In this study, the differences between the two- and three-step PE-ALE processes of SiN with and without O2 plasma irradiation are discussed. In the simulations of this study, low-energy CH2F radicals are deposited on a SiN surface in the adsorption step, and the modified surface is then irradiated with energetic Ar+ ions and removed in the desorption step. In the O2 plasma irradiation step, the resulting surface is irradiated with low-energy O species, and excess carbon (C) atoms are removed. It has been found that the O irradiation step effectively prevents the accumulation of C atoms by promoting the formation of volatile CO molecules.

Assessment of polyhydroxyalkanoates and polysaccharides production in native phototrophic consortia under nitrogen and phosphorous-starved conditions

Growing demand for sustainable and eco-friendly alternatives to petroleum-based polymers has increased the interest in the microalgae-based production of polymers, specifically polyhydroxyalkanoates and polysaccharides. While most studies in microbial polymer production have primarily focused on axenic or genetically engineered cultures of cyanobacteria and eukaryotic algae, little is known about the potential of mixed phototrophic consortia. This study aimed to obtain and evaluate mixed photosynthetic consortia of different origins (natural and residual) as a novel approach for polyhydroxyalkanoates and polysaccharides accumulation. Activated sludge and freshwater samples were collected and inoculated in lab-scale photobioreactors to generate mixed photosynthetic consortia. After a preliminary screening for polymer-accumulating strains under nutrient-unbalanced conditions, the selected strains were subjected to a biphasic strategy (biomass accumulation and nutrient stress) to evaluate their polyhydroxyalkanoates and polysaccharide accumulation. First, cultures were subjected to a nutrient-rich phase to increase the biomass content and then deprived of nutrients (known as the polymer accumulation phase) to evaluate polyhydroxyalkanoates and polysaccharide yield. Findings in this study revealed that the highest polysaccharide yield for activated sludge biomass and freshwater consortia was 460 ± 16 and 320 ± 24 mg glucose g dried biomass−1, respectively. In contrast, the highest polyhydroxyalkanoates accumulation levels for both cultures were calculated at 5 mg polyhydroxyalkanoates g dried biomass−1. The efficacy of nutrient stress as a selective pressure strategy to develop mostly polysaccharides-accumulating consortia was demonstrated.

Exploring the Role of Green Microbes in Sustainable Bioproduction of Biodegradable Polymers.

Research efforts have shifted to creating biodegradable polymers to offset the harmful environmental impacts associated with the accumulation of non-degradable synthetic polymers in the environment. This review presents a comprehensive examination of the role of green microbes in fostering sustainable bioproduction of these environment-friendly polymers. Green microbes, primarily algae and cyanobacteria, have emerged as promising bio-factories due to their ability to capture carbon dioxide and utilize solar energy efficiently. It further discusses the metabolic pathways harnessed for the synthesis of biopolymers such as polyhydroxyalkanoates (PHAs) and the potential for genetic engineering to augment their production yields. Additionally, the techno-economic feasibility of using green microbes, challenges associated with the up-scaling of biopolymer production, and potential solutions are elaborated upon. With the twin goals of environmental protection and economic viability, green microbes pave the way for a sustainable polymer industry.

Novel eco-friendly polylactic acid nanocomposite integrated membrane system for sustainable wastewater treatment: Performance evaluation and antifouling analysis

Water contamination caused by heavy metals, nutrients, and organic pollutants of varying particle sizes originating from domestic and industrial processes poses a significant global challenge. There is a growing concern, particularly regarding the presence of heavy metals in freshwater sources, as they can be toxic even at low concentrations, posing risks to human health and the environment. Currently, membrane technologies are recognized as effective and practical for treating domestic and industrial wastewater. However, these technologies are hindered by fouling issues. Furthermore, the utilization of conventional membranes leads to the accumulation of non-recyclable synthetic polymers, commonly used in their production, resulting in adverse environmental consequences. In light of our previously published studies on environmentally friendly, biodegradable polylactic acid (PLA) nanocomposite mixed matrix membranes (MMMs), we selected two top-performing PLA-based ultrafiltration nanocomposite membranes: one negatively charged (PLA-M−) and one positively charged (PLA-M+). We integrated these membranes into systems with varying arrangements to control fouling and eliminate heavy metals, organic pollutants, and nutrients from raw municipal wastewater collected by the local wastewater treatment plant in Abu Dhabi (UAE). The performance of two integrated systems (i.e., PLA-M+/PLA-M− and PLA-M−/PLA-M+) was compared in terms of permeate flux, contaminant removal efficiencies, and fouling mitigation. The PLA-M+/PLA-M− system achieved removal efficiencies of 79.6 %, 92.6 %, 88.7 %, 85.2 %, 98.9 %, 94 %, 83.3 %, and 98.3 % for chemical oxygen demand (COD), nitrate (NO3−-N), phosphate (PO43−-P), ammonium (NH4+-N), iron (Fe), zinc (Zn), nickel (Ni), and copper (Cu), respectively. On the other hand, the PLA-M−/PLA-M+ system recorded removal efficiencies of 85.8 %, 95.9 %, 100 %, 81.9 %, 99.3 %, 91.9 %, 72.9 %, and 98.9 % for COD, NO3−-N, PO43−-P, NH4+-N, Fe, Zn, Ni, and Cu, respectively. Notably, the PLA-M−/PLA-M+ system demonstrated superior antifouling resistance, making it the preferred integrated system. These findings demonstrate the potential of eco-friendly PLA nanocomposite UF-MMMs as a promising alternative to petroleum-based polymeric membranes for efficient and sustainable wastewater treatment.

Isolation, Characterisation and Evaluation of Plastic Degrading Properties of Soil Biofilms Collected from Chennai District

Environmental pollution due to accumulation of synthetic polymers namely plastics is a growing concern which threatens the terrestrial marine flora and fauna. Traditional methods of plastic disposal include incineration and disposal into landfills or water bodies. Incineration of polyethylene, polystyrene leads to emission of a large amount of carbon monoxide which is toxic if inhaled and also a potent greenhouse gas. Degradation of plastic by microorganisms is an efficient and eco-friendly method employed for rapid rate of disintegration. The biofilm present in the contaminated soil survives by adapting to harsh environment by secreting hydrolysing enzymes which are potent in degradation of the accumulated plastics. The present study deals with the isolation, characterisation and evaluation of plastic degrading properties of microorganisms isolated from various soil samples collected from Chennai district. Soil samples were collected aseptically from various locations & isolated by standard plate count method. The isolated organisms were identified by staining methods and characterized by phylogenetic analysis. The organism Pseudomonas aeruginosa and Streptomyces fulvissimus were further subjected to plastic degradation testing. The present study demonstrates the ability of Pseudomonas aeruginosa and Streptomyces fulvissimus to degrade polyethylene sheets.