Absolute Charge Research Articles

Simulation and commissioning of a Faraday cup for absolute charge measurements of very high-energy electrons in-air at PEER

The Pulsed Energetic Electrons for Research (PEER) beamline at the ANSTO Australian Synchrotron comprises a 100 MeV linac injector that is currently being developed for ultra-high dose-rate, very high-energy electron radiotherapy research. Previously, dosimetry studies discovered a lack of reliable charge measurement to the in-air end station, though no change in response was recorded in fast current transformer measurements, the only available diagnostic device for measuring charge. This work describes the process of simulating and then commissioning a purpose-built Faraday cup to ascertain absolute in-air charge measurements at PEER. By combining simulation data with experimental results, the PEER Faraday cup is shown to possess a primary electron capture efficiency of (99.22 ± 0.10)%, with a net capture efficiency due to secondary electron emission of (97.87 ± 0.24)%. These results show the PEER Faraday cup performs as intended and, when scaled against simulations, will be suitable for measuring absolute charge at PEER.

Absolute nuclear charge radius by Na-like spectral line separation in high-Z elements

Abstract We describe a novel technique to determine absolute nuclear radii of high-Z nuclides. Utilizing accurate theoretical atomic structure calculations together with precise measurements of extreme ultraviolet transitions in highly charged ions this method allows for precise determinations of absolute nuclear charge radii based upon the well-known nuclear radii of their neighboring elements. This method can work for elements without stable isotopes, and its accuracy may be competitive with current methods (electron scattering and muonic x-ray spectroscopy).

Addressing accuracy by prescribing precision: Bayesian error estimation of point defect energetics

With density functional theory (DFT), it is possible to calculate the formation energy of charged point defects and in turn to predict a range of experimentally relevant quantities, such as defect concentrations, charge transition levels, or recombination rates. While prior efforts have led to marked improvements in the accuracy of such calculations, comparatively modest effort has been directed at quantifying their uncertainties. However, in the broader DFT research space, the development of Bayesian Error Estimation Functionals (BEEF) has enabled uncertainty quantification (UQ) for other properties. In this paper, we investigate the utility of BEEF as a tool for UQ of defect formation energies. We build a pipeline for propagating BEEF energies through a formation-energy calculation and test it on intrinsic defects in several materials systems spanning a variety of chemistries, bandgaps, and crystal structures, comparing to prior published results where available. We also assess the impact of aligning to a deep-level transition rather than to the VBM (valence band maximum). We observe negligible dependence of the estimated uncertainty upon a supercell size, though the relationship may be obfuscated by the fact that finite-size corrections cannot be computed separately for each member of the BEEF ensemble. Additionally, we find an increase in estimated uncertainty with respect to the absolute charge of a defect and the relaxation around the defect site without deep-level alignment, but this trend is absent when the alignment is applied. While further investigation is warranted, our results suggest that BEEF could be a useful method for UQ in defect calculations.

(099) HOW MUCH IS ENOUGH FOR BOTOX(R) & HOW MUCH BOTOX(R) IS ENOUGH? A REVIEW OF BOTULINUM TOXIN INJECTIONS FOR THE TREATMENT OF HYPERTONIC PELVIC FLOOR

Abstract Introduction Botulinum toxin (BT) is a useful adjunct in the treatment of hypertonic pelvic floor muscles. By blocking the release of acetylcholine, a necessary neurotransmitter for muscle contraction, it facilitates muscle relaxation. These paralytic effects effectively disrupt painful muscle spasms and help patients make further progress in physical therapy when otherwise hindered by painful muscle spasms. Despite having robust data to support the off-label use of BT for the treatment of myofascial pain, there is limited data about its use for the treatment of pelvic floor myofascial pain. Additionally, there is no data about insurance reimbursement or the financial costs to patients undergoing pelvic floor muscle treatment with BT injections. Objective 1. Determine how many injections of BT patients receive for the treatment of hypertonic pelvic floor 2. Determine insurance reimbursement and financial costs to patients undergoing pelvic floor muscle treatment with BT injections. Methods Data was first collected by building a report in Epic to identify all patients seen in our department between 1/1/2022 to 10/31/2023 for a BT-related visit. Patient were further stratified based on CPT code 96372, indicating that they received pelvic floor BT injections. Fifty-one patients were identified. Charts were reviewed to determine how frequently patients returned for BT administration & to determine how much patients had to pay for BT treatment after filing with insurance. Results A total of 51 patients received pelvic floor muscle BT injections over the course of 61 patient encounters. Forty-three patients (84%) received BT once, 6 patients (12%) received BT twice, and 2 patients (4%) received BT 3 times. Of the 8 patients who required additional BT treatment, the average time between subsequent BT injections was 83 days. At our institution, the absolute charge for 100 units of BT was $1,068.16 without insurance. All patients treated had active insurance, and all 61 patient encounters were billed through insurance. At the time of this review, 20 patients encounters (33%) were still pending review with insurance, 41 patient encounters (67%) had been reviewed. Of these 41 patient encounters, 30 (73%) were accepted through insurance; 11 (27%) were denied. Nineteen administrations of BT were completely covered by insurance (no self-pay); 11 administrations of BT were partially covered through insurance. For patients that received partial or total coverage through their insurance company, the average self-pay cost for 100 units of BT was $66.77. Conclusions Most patients required one visit for pelvic floor muscle treatments with BT, and only occasionally required more than one visit. Prospective studies are needed to quantify and quality patient’s improvement in pain following each BT treatment. In the majority of cases, when BT was filed through insurance, it was at least partially approved. Without insurance, the charge for 100 units of BT was $1,068; when approved through insurance, the average self-pay cost for 100 units of BT was $66.77. Disclosure Any of the authors act as a consultant, employee or shareholder of an industry for: Patty Brisben Foundation.

MMC Array to Study X-Ray Transitions in Muonic Atoms

The QUARTET collaboration aims to significantly improve the precision of the absolute nuclear charge radii of light nuclei from Li to Ne by using an array of metallic magnetic calorimeters to perform high-precision X-ray spectroscopy of low-lying states in muonic atoms. A proof-of-principle measurement with lithium, beryllium and boron is planned for fall 2023 at the Paul Scherrer Institute. We discuss the performance achieved with the maXs-30 detector module to be used. To place the detector close to the target chamber where the muon beam will impact the material under study, we have developed a new dilution refrigerator sidearm. We further discuss the expected efficiency given the transparency of the X-ray windows and the quantum efficiency of the detector. The expected muonic X-ray rate combined with the high resolving power and detection efficiency of the detector suggest that QUARTET will be able to study the de-excitation of light muonic atoms at an unprecedented level, increasing the relative energy resolution by up to a factor of 20 compared to conventional detector techniques.

Orbital-angular-momentum-dependent speckles for spatial mode sorting and demultiplexing

Characterizing the orbital-angular-momentum (OAM) modes is critically important for OAM-encoded data transfer. However, traditional characterizing approaches rely on either complex and precise experimental configurations or complicated prior information processing. In these scenarios, the correlation features of OAM-dependent speckles from the scattering effect have received little attention. Here, we investigate the cross-correlation characteristics of the OAM speckles resulting from a scattering medium and propose an appealing alternative for spatial mode sorting and demultiplexing based on the OAM-dependent speckles. We demonstrate theoretically and experimentally that the cross-correlation operation between two different OAM-dependent speckles can uniformly derive an annulus pattern, whose dimension is determined by the absolute topological charge difference value between the two corresponding OAM modes. Based on this mechanism, the original coherent OAM modes can be easily sorted by implementing the cross-correlation operation between incoherently measured OAM-dependent speckles. To fully capitalize on the orthogonal feature of the OAM-dependent speckles, we also construct OAM mode demultiplexing experiments using a ground glass diffuser, where both 8-bit grayscale and 24-bit RGB OAM-encoded data demultiplexing are successfully demonstrated with superior error rates. Results show that the OAM-dependent speckles, previously treated as a nuisance for practical applications, can be surprisingly utilized as a competitive candidate for OAM mode sorting and demultiplexing, thus opening new directions in optical communication and information processing.

Position-sensitive non-destructive detection of charged-particle bunches in low-energy beamlines

We have developed and operated an electronic detection system for the non-destructive single-pass detection of bunches of charged particles in a beamline that allows for a measurement of their lateral position with respect to the central beamline axis on a shot-to-shot basis. It provides all features of our related development reported in Kiffer et al. (Rev Sci Instrum 90:113301, 2019), namely single-pass measurement of bunch length, kinetic energy and absolute charge, and is additionally designed to provide the lateral position of bunches with sub-mm accuracy. We show the setup, associated methods and provide characterizing measurements with bunches of highly charged ions in the keV regime of kinetic energy that demonstrate the capabilities and show a typical application.

Heparin-Immobilized Polyethersulfone for Hemocompatibility Enhancement of Dialysis Membrane: In Situ Synchrotron Imaging, Experimental, and Ex Vivo Studies.

The goal of the current study is to enhance the hemocompatibility of polyethersulfone (PES) membranes using heparin immobilization. Heparin was immobilized covalently and via electrostatic interaction with the positively charged PES surface (pseudo-zwitterionic (pZW) complex) to investigate the influence of each method on the membrane hemocompatibility. In situ synchrotron radiation micro-computed tomography (SR-µCT) imaging, available at the Canadian Light Source (CLS), was used to critically assess the fibrinogen adsorption to the newly synthesized membranes qualitatively and quantitatively using an innovative synchrotron-based X-ray tomography technique. The surface roughness of the synthesized membranes was tested using atomic force microscopy (AFM) analysis. The membrane hemocompatibility was examined through the ex vivo clinical interaction of the membranes with patients' blood to investigate the released inflammatory biomarkers (C5a, IL-1α, IL-1β, IL-6, vWF, and C5b-9). The presence and quantitative analysis of a stable hydration layer were assessed with DSC analysis. Surface modification resulted in reduced surface roughness of the heparin-PES membrane. Both types of heparin immobilization on the PES membrane surface resulted in a decrease in the absolute membrane surface charge from -60 mV (unmodified PES) to -13 mV for the pZW complex and -9.16 mV for the covalently attached heparin, respectively. The loss of human serum fibrinogen (FB) was investigated using UV analysis. The PES membrane modified with the heparin pseudo-ZW complex showed increased FB retention (90.5%), while the unmodified PES membrane and the heparin covalently attached PES membrane exhibited approximately the same level of FB retention (81.3% and 79.8%, respectively). A DSC analysis revealed an improvement in the content of the hydration layer (32% of non-freezable water) for the heparin-coated membranes compared to the unmodified PES membrane (2.84%). An SR-µCT analysis showed that the method of heparin immobilization significantly affects FB adsorption distribution across the membrane thickness. A quantitative analysis using SR-µCT showed that when heparin is attached covalently, FB tends to be deposited inside the membrane pores at the top (layer index 0-40) membrane regions, although its content peak distribution shifted to the membrane surface, whereas the unmodified PES membrane holds 90% of FB in the middle (layer index 40-60) of the membrane. The ex vivo hemocompatibility study indicates an improvement in reducing the von Willebrand factor (vWF) for the heparin pseudo-ZW PES membrane compared to the covalently attached heparin and the untreated PES.

Application of selected chemometric methods to describe and predict the properties of grafted ceramic membranes

Modification of a ceramic membrane surface for the purpose of hydrophobization can be achieved by the grafting technique involving the application of silane compounds and their derivatives. For the selection of the appropriate modifying compound, chosen chemometric methods can be implemented. In this study, the chemometric methods, i.e., hierarchical cluster analysis (HCA) and principal component analysis (PCA), were used for the evaluation of the properties of selected silane compounds applied as modifiers of ceramic membranes.The results of the calculations indicate that these methods are effective in predicting the properties of modifiers. Moreover, the PCA results provide additional information about the physicochemical properties of modifiers which influence grafted membrane properties. The data are essential when looking for (or synthesizing) new modifiers/linkers necessary for the preparation of ceramic membranes with the desirable properties It was found that physicochemical parameters such as molecular shape, atomic mass, total absolute charge, number of heavy atoms, polarizability, van der Waals volume, electronegativity as well as the number of H-bond acceptors are the most important. These parameters describe the observed properties of the grafting molecules and, simultaneously, the properties of the obtained modified ceramic membranes. It was also demonstrated that HCA and PCA can be used to interpret the previously obtained experimental results. For instance, HCA can limit the number of consecutive experiments aimed at estimating the stability of membranes in various organic solvents. Moreover, the applicability of multiple linear regression (MLR) analysis to predict the values of contact angle (CA) and water fluxes of modified membranes was also examined.

Electrokinetic ion enrichment in asymmetric charged nanochannels

Artificial bionic nanochannels have attracted wide attention and successfully used in various fields. In this work, a novel nanochannel with asymmetric surface charge is proposed to investigate the ion enrichment effect. The results show that the proposed nanochannel has excellent ion enrichment performance and the obtained ion enrichment ratio is up to 1500 when the ion concentration is 0.01 mM, which is much higher than precedent researches typically ranging from tens to hundreds. Besides, we found that the forward voltage bias will produce ions enrichment and the reverse voltage bias will produce ions depletion. The ion enrichment ratio is higher at the larger voltage bias, absolute surface charge density and smaller nanochannel height. In addition, the ion enrichment performance is more sensitive to the change of charged wall length and not sensitive to the change of uncharged wall length. The research report offers important information and instructions for the design and optimum on ion enrichment performance.

Muonic x-ray spectroscopy on implanted targets

Muonic x-ray spectroscopy uses muons to obtain information about the structure of the atom and the nucleus. In muonic atoms, the energy levels of atomic orbitals are significantly more sensitive to the finite size correction. By probing these orbitals using x-ray spectroscopy, the nuclear size correction can be extracted, providing valuable input for laser spectroscopy in the form of absolute charge radii with a relative precision better than 10−3. Continuing on developments that allowed measurements on target quantities of about 5 μg, we showed the feasibility of using implanted targets. In the future, this will allow the measurement of absolute charge radii of long-lived radioactive isotopes that are not available in sufficient enrichment or large quantities. In this contribution, we shall report on the target preparation, involving high-fluence implantation, and on the preliminary results of the muX experimental campaign.

Mechanism and Kinetics of Prothioconazole Photodegradation in Aqueous Solution.

This study investigated the effects of light source, pH value, and NO3- concentration on the photodegradation of prothioconazole in aqueous solution. The half-life (t1/2) of prothioconazole was 173.29, 21.66, and 11.18 min under xenon, ultraviolet, and high-pressure mercury lamps, respectively. At pH values of 4.0, 7.0, and 9.0 under a xenon lamp light source, the t1/2 values were 693.15, 231.05, and 99.02 min, respectively. Inorganic substance NO3- clearly promoted the photodegradation of prothioconazole, with t1/2 values of 115.53, 77.02, and 69.32 min at NO3- concentrations of 1.0, 2.0, and 5.0 mg L-1, respectively. The photodegradation products were identified as C14H15Cl2N3O, C14H16ClN3OS, C14H15Cl2N3O2S, and C14H13Cl2N3 based on calculations and the Waters compound library. Furthermore, density functional theory (DFT) calculations showed that the C-S, C-Cl, C-N, and C-O bonds of prothioconazole were the reaction sites with high absolute charge values and greater bond lengths. Finally, the photodegradation pathway of prothioconazole was concluded, and the variation in energy of the photodegradation process was attributed to the decrease in activation energy caused by light excitation. This work provides new insight into the structural modification and photochemical stability improvement of prothioconazole, which plays an important role in decreasing safety risk during application that will reduce the exposure risk in field environment.

Controlled supramolecular interactions for targeted release of Amiodarone drug through Graphyne to treat cardiovascular diseases: An in silico study

In the current study, the drug loading ability of graphyne (GY) for the amiodarone (AMD) drug is investigated for the first time. The efficacy of GY as a carrier for amiodarone (a cardiovascular drug) is evaluated by calculating its electronic, energetic, optimized, and excited state properties with help of the density functional theory (DFT). The AMD drug interacted with the GY molecule with an adsorption energy of about −0.19 eV (gas-phase) and −1.92 eV (aqueous phase), suggesting that the AMD@GY complex is stable in water-phase. The HOMO (highest-occupied molecular-orbital) of the AMD@GY complex is concentrated on the AMD drug while the LUMO (lowest-unoccupied molecular-orbital) is centralized on GY with absolute charge separation, indicating charge transfer will occur between AMD and GY. The charge-transfer process is further studied with the aid of charge-decomposition analysis (CDA). The non-covalent interaction analysis (NCI) exposed that non-covalent forces exist between the GY carrier and AMD drug. These non-covalent forces between AMD drug and GY carrier play a significant role in drug unloading at the targeted or diseased site. Likewise, the calculations at excited-state, charge-state (+1 and −1) influence on GY and AMD@GY complex structures, and photo-induced electron transfer analysis (PET) are also studied for the graphyne-based drug-delivery system. According to PET and electron-hole analysis, fluorescence-quenching will occur upon interaction. Overall, it is concluded that graphyne can be exploited as a drug carrier for amiodarone drug delivery. Researchers will be fascinated to look at alternative 2D nanomaterials for drug delivery applications as a result of this theoretical work.

Effects of FeII, tannic acid, and pH on the physicochemical stability of oil body emulsions

This study examined the effects of solution pH (3.0, 5.0, and 7.0), FeII, and tannic acid (TA) on oil body (OB) emulsions and monitored changes in emulsion particle size, zeta potential, appearance, microstructure, and oxidation stability over 14 days of storage at 37 °C. The chelation efficiency, zeta potential, and fourier-transform infrared spectroscopy results showed that the ability of TA to chelate FeII and the absolute charge of TA-Fe complexes increased with the pH. In addition, the distribution of these complexes in the emulsions was influenced by their electrostatic and hydrogen bonding interactions with the OB. At pH 3.0 and 5.0, the TA-Fe and OB emulsion particles attracted each other, resulting in droplet flocculation. At pH 7.0, the TA-Fe and OB emulsion particles carried the same (negative) charge, and had little effect on physical stability of OB emulsions. TA generally inhibited the oxidation of OB emulsions but promoted the concentration of the primary and secondary oxidation products (61.48 mmol/kg and 50.5 μmol/kg, respectively) of FeII-containing emulsions at pH 3.0. The obtained results provide new insights into the efficacy of TA as a natural antioxidant for emulsified foods and thus pave the way to increasing their quality and shelf life.

Muonic atom spectroscopy with microgram target material

Muonic atom spectroscopy–the measurement of the x rays emitted during the formation process of a muonic atom–has a long standing history in probing the shape and size of nuclei. In fact, almost all stable elements have been subject to muonic atom spectroscopy measurements and the absolute charge radii extracted from these measurements typically offer the highest accuracy available. However, so far only targets of at least a few hundred milligram could be used as it required to stop a muon beam directly in the target to form the muonic atom. We have developed a new method relying on repeated transfer reactions taking place inside a 100 bar hydrogen gas cell with an admixture of 0.25% deuterium that allows us to drastically reduce the amount of target material needed while still offering an adequate efficiency. Detailed simulations of the transfer reactions match the measured data, suggesting good understanding of the processes taking place inside the gas mixture. As a proof of principle we demonstrate the method with a measurement of the 2p-1s muonic x rays from a 5 upmu hbox {g} gold target.

A deep learning method for the trajectory reconstruction of cosmic rays with the DAMPE mission

A deep learning method for the particle trajectory reconstruction with the DAMPE experiment is presented. The developed algorithms constitute the first fully machine-learned track reconstruction pipeline for space astroparticle missions. Significant performance improvements over the standard hand-engineered algorithms are demonstrated. Thanks to the better accuracy, the developed algorithms facilitate the identification of the particle absolute charge with the tracker in the entire energy range, opening a door to the measurements of cosmic-ray proton and helium spectra at extreme energies, towards the PeV scale, hardly achievable with the standard track reconstruction methods. In addition, the developed approach demonstrates an unprecedented accuracy in the particle direction reconstruction with the calorimeter at high deposited energies, above several hundred GeV for hadronic showers and above a few tens of GeV for electromagnetic showers.

Absolute charge measurements for ps-long bunches using a wall current monitor in linac-based free electron lasers

Wall current monitors (WCMs) are devices used to measure the bunch charge or current profile of a charged particle beam. They are commonly perceived as absolute charge measurement devices, which is not really true, since the pickup response at DC is zero. A charge value has to be determined from the variation of the output signal. For very short bunches of picosecond length, this becomes more and more affected by ferrite losses and the general wide band frequency behaviour of the WCM geometry. In this paper, a new method is proposed to determine the absolute bunch charge of a few picosecond-long electron bunch by analysing and processing the WCM waveforms. We present a detailed characterization of the WCM in operation at SwissFEL by means of an equivalent circuit model, numerical methods and test bench measurements. The results thus obtained are compared with charge measurements obtained from other monitors operating along the SwissFEL linear accelerator. The extensive characterization of the WCM allowed in combination with the waveform processing technique to derive, for the first time, the absolute charge of a single ps-long electron bunch.

Study of EPR-based nanodielectrics under operational conditions for DC cable insulation

A model DC material based on ethylene propylene rubber (EPR) including the pure EPR and the EPR-based nanodielectrics incorporated with two different nanoclays, Kaoline and Talc, under operational conditions was investigated. The operational conditions include a 20 kV/mm electric field at 25 °C, a 20 kV/mm electric field at 50 °C with a thermal gradient, and a 40 kV/mm electric field at 50 °C with a thermal gradient and polarity reversal. Space charge distribution, surface potential, and electrical conductivity were measured to characterize the model DC material and interpret the discrete charge dynamics in the bulk and at the interface of the three samples. The experimental results revealed that the electrical conductivity of Talc-filled nanodielectric has the least dependency on electric field and temperature, which reduces the conductivity gradient across the dielectric. Moreover, the successful suppression of space charge and the lower dielectric time constant in the Talc-filled nanodielectric result in a tuning electric field in the bulk not only under normal operating conditions but also more importantly under polarity reversal conditions. The maximum of absolute charge density decreases from 10.6 C/m3 for EPR to 2.9 C/m3 for the Talc-filled nanodielectric under 40 kV/mm with polarity reversal and at 50 °C with the thermal gradient. The maximum of local electric field enhancement for the mentioned condition reduces significantly from 97 kV/mm, 142% enhancement, for EPR to 45 kV/mm, only 12.5% enhancement, for the Talc-filled nanodielectric.

Rapid Opto-electrochemical Differentiation of Marine Phytoplankton.

The use of electro-generated oxidants in seawater facilitates the discrimination of different plankton groups via monitoring the decay in real time of their chlorophyll-a (chl-a) fluorescence signals following potentiostatic initiation of electrolysis in their vicinity (Yang M.Chem. Sci.2019, 10( (34), ), 7988-7993). In this paper, we explore the sensitivity of phytoplankton to different chemical species produced at various potentials in seawater. At low potentials, the oxidation of ca. millimolar bromide naturally present in seawater to hypobromous acid 'switches-off' the chl-a signal of individual Chlamydomonas concordia cells (green algae) located on the electrode surface within tens of seconds of the potential onset. At higher oxidative potentials, the oxidation of chloride and water produces oxidants (Cl2, OH, H2O2, etc.) that are also lethal to the plankton. To deconvolute the contributions to the response from the chemical identity of the oxidant and the amount of charge delivered to 'titrate' the individual living plankton using the loss of fluorescence as the 'end point', we introduce a ramped galvanostatic method. This approach enabled the controlled injection of charge applied to a bespoke electrochemical cell in which the plankton are immobilized on an electrode surface for rapid and sensitive measurement. It is shown that the number of moles (charge) of oxidants required to react leading to chl-a switch-off is independent of the chemical identity of the electro-generated oxidant(s) among hypobromous acid, chlorine, or water-derived oxidants. Comparative experiments between C. concordia and Emiliania huxleyi (where the latter are encapsulated by extracellular plates of calcium carbonate) show that significantly different amounts of absolute charge (moles of electro-generated oxidants) are required in each case to 'switch-off' the chl-a signal. The method provides the basis for a tool that could distinguish between different plankton cells within ca. 2 min including the setup time.

Effect of enzymatic hydrolysis conditions on structure of soy protein isolate/gum arabic complex and stability of oil-in-water emulsion.

The emulsifying, antioxidant and foaming properties of soy protein isolate hydrolysates (SPH) can be improved by the addition of gum arabic (GA). We investigated the effects of different hydrolysis conditions on the complexation of SPH and GA, and the effects of the complex on the properties of emulsions. Fluorescence spectroscopy showed that the addition of GA had a stronger effect on bromelain and pepsin hydrolysates than trypsin hydrolysate, and therefore had a higher binding constant (KA ) and a larger number of binding sites (n). The addition of GA could also improve protein solubility and emulsifying ability. The emulsions prepared with complexes, especially the complex of GA and SPH obtained by pepsin hydrolysis for 3h, had a high absolute charge value, uniform particle size distribution, stable morphology, and good storage stability. After storage, the emulsification index (CI) of the emulsion only increased to 23.08%; its 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity was 24.37 ± 1.22% and its 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS+ ) free radical scavenging activity was largely retained. During long-term storage, pepsin-treated protein (especially protein treated for 3h) and GA can form a stable emulsion with antioxidant properties. This work provides new ideas for the development of natural and safe emulsifiers that have antioxidant properties and can be stored long-term and used in the food industry. © 2022 Society of Chemical Industry.