AB Group Research Articles

Effect of antibiotics as an adjuvant to subgingival instrumentation on systemic inflammation in patients with periodontitis - A randomized clinical trial.

The aim of the present study was to evaluate effect of subgingival instrumentation (SI) with or without antibiotics on systemic inflammation. Moreover, systemic parameters were compared between periodontally healthy (PH) individuals and periodontitis patients. Patients with generalized periodontitis: stage III and PH individuals were recruited. Forty eight periodontitis patients were randomly allocated to each treatment group; systemic antibiotics for seven days after completion of SI (AB group), or SI alone (SI group). Periodontal parameters, serum high-sensitivity C-reactive protein (hsCRP), and haematological parameters were assessed at baseline and at 8th week. Multivariate analysis was applied to analyze predictive effect of treatment allocated and improvement in periodontal parameters on change in systemic parameters. At baseline, hsCRP, total leukocyte count (TLC), neutrophil, and monocyte count were significantly higher in periodontitis patients. There was comparable reduction in neutrophil count in both treatment groups. At 8th week, change in periodontal parameters was similar in treatment groups, except for probing pocket depth (PPD). Improvement in both PPD and clinical attachment level (CAL) and CAL alone was predictive of change in TLC and lymphocyte count respectively. This study failed to demonstrate the significant benefit of systemic antibiotics as an adjuvant to SI on improvement in periodontal inflammation and systemic inflammatory parameters, despite significantly higher reduction in PPDs.

Importance of Interrelation between ABO Blood Group and Platelet Indices in Young Healthy Adults

Objective: To highlight the importance of the interrelation between the ABO blood group system and platelet indices in healthy young adults.
 Study Design: Cross-sectional study
 Place and Duration of Study: Pathology Department, PNS Shifa Hospital, Karachi Pakistan, from Jan to Jun 2020.
 Methodology: Comprised 399 blood donors who visited the transfusion department of the hospital without any comorbidities.All the donors were requested for their informed consent before their enrolment in the study. Blood groups were determined by a simple conventional tile method. Platelet indices (Platelet count, mean platelet volume and Platelet distribution width)were studied by auto-analyzer. Blood pressures were estimated by manual auscultator technique with a mercury sphygmomanometer.
 Results: Males and females were equal in number, with a mean BMI of 19.89(1.24%). The majority of the donors, 143(35.8%),had B-Blood Group, while 140(35.1%) donors had O-Blood group, 93(23.3%) had A-Blood group, and only 23(5.8%) had AB group. Rh positivity was noted in 378(94.7%) of the donors. Different blood groups were responsible for the changes in the platelet parameters, especially MPV and PDW, as there was a significant difference in MPV and PDW values (p-value<0.05).
 Conclusion: While determining the presence of a relationship between the ABO blood group system and platelet indices, it is observed that there is a high frequency of cardiovascular diseases among B and AB blood groups in young, healthy adults.Keywords: Blood groups, Body mass index, Mean platelet volume, Platelet count, Platelet distribution width.

Increased cytotoxic natural killer cells in the endometrium alone cannot be considered the immunological cause of recurrent miscarriage

To study the distribution and gene expression of endometrial immune cell populations, especially natural killer (NK) subsets, between assisted reproductive technology patients and healthy donors and explore a possible relationship of these results with patients' killer cell immunoglobulin-like receptor (KIR) genotypes and KIR-human antigen leukocyte-C (HLA-C) binding. Prospective observational cohort study. Clinic and university laboratories. Participants included 39 women with recurrent miscarriages who had undergone invitro fertilization cycles with donated oocytes and 21 healthy oocyte donors with proven fertility. Endometrial biopsy samples were collected from both patients and donors, and the KIR genotypes of the assisted reproductive technology patients were analyzed. Endometrial gene expression (cluster of differentiation [CD] antigens and anti-inflammatory and proinflammatory interleukins) and the number and percentage of regulatory T and NK cell populations in patients and donors were determined. Subsequently, the results obtained were categorized in the group of patients by KIR genotype. Killer cell immunoglobulin-like receptor-HLA-C binding was also examined in patients, considering their KIRs. A higher percentage of CD56dimCD16+ NK cells were observed in patients than those in healthy donors. Nevertheless, when categorizing patients by KIR genotype and comparing the KIR AA (35.9%), AB (43.6%), and BB (20.5%) groups, no statistically significant difference was observed in either endometrial gene expression or any of the immune cell populations analyzed. Finally, no differences in binding between KIR and HLA-C molecules were registered among these 3 sets of patients. The reported increase in the number of NK cells with a cytotoxic profile in the endometrium of women with a history of recurrent miscarriages cannot alone explain these events because no relationship is observed between such cellular increase and the KIR genotypes, which individually, and in combination with the different HLA-C alleles, have also been associated, by previous studies, with negative reproductive outcomes. 1405-MAD-025-JG.

The effects of surface treatments on electron beam melted Ti-6Al-4V disks on osteogenesis of human mesenchymal stromal cells

Additive manufactured (AM) Titanium-6Aluminum-4Vanadium (Ti64) scaffolds display unique mechanical and biological properties for implant devices. The elastic modulus can be tailored by adjusting the porosity, further facilitating bone ingrowth. Although Ti64 implants are biocompatible, the effects of AM surfaces without porous structures, and how the topography and surface chemistry of the respective surfaces affect the osteogenesis of bone marrow-derived mesenchymal stromal cells (BMSCs) has not yet been revealed. In this paper, we cultured BMSCs on solid electron beam melted Ti64 disks subjected to three surface treatments: chemical etching (HF), atomic-layer deposition of TiO2 (TiO2), and polished (POL), or left untreated (AB). The biocompatibility and osteogenic properties of these surfaces were investigated, and the results were compared to cells cultured in regular tissue-culture polystyrene culturing wells (TCPS). The surfaces were hydrophobic, except for the polished surface which was hydrophilic.All surface treatments are biocompatible and allow for osteogenic differentiation, as revealed by viability assays and gene expression analysis. Scanning electron microscopy shows that cells adhere differently depending on the surface properties, with more filopodia on the rougher surfaces, AB and TiO2 disks, and more lamellipodia on the smoother surfaces, HF and POL disks. All groups stimulated with beta glycerophosphate, ascorbic acid, and dexamethasone, have elevated expression of genes related to matrix formation, where the cells cultured on the disks treated with TiO2, HF and POL have the overall highest expression. The AB group appears to be less favorable in regards to matrix formation. Considering the matrix mineralization, the rougher surfaces, AB and TiO2, are able to induce matrix mineralization, with an elevated gene expression of vitamin D receptors and calcium deposition of unstimulated cells. Finally, imaging at day 21 revealed an even amount of cells and matrix, covering most of the partially melted particles. Our results suggests that surface topography is more important to osteogenesis than the wettability of the surface. Overall, the present study contributes to the understanding of using surface modifications to AM Ti64 implant materials and reveals how they affect bone growth.

HLA and red blood cell antigen genotyping in SARS-CoV-2 convalescent plasma donors.

Aim: More data is required regarding the association between HLA allele and red blood cell (RBC) antigen expression in regard to SARS-CoV-2 infection and COVID-19 susceptibility. Methods: ABO, RhD, 37 other RBC antigens and HLA-A, B, C, DRB1, DQB1 and DPB1 were determined using high throughput platforms in 90 Caucasian convalescent plasma donors. Results: The AB group was significantly increased (1.5×, p=0.018) and some HLA alleles were found to be significantly overrepresented (HLA-B*44:02, C*05:01, DPB1*04:01, DRB1*04:01 and DRB1*07:01) or underrepresented (A*01:01, B51:01 and DPB1*04:02) in convalescent individuals compared with the local bone marrow registry population. Conclusion: Our study of infection-susceptible but non-hospitalized Caucasian COVID-19 patients contributes to the global understanding of host genetic factors associated with SARS-CoV-2 infection and severity.

The prevalence, presentation and outcome of colistin susceptible-only Acinetobacter Baumannii-associated pneumonia in intensive care unit: a multicenter observational study

Hospital-acquired pneumonia (HAP) and ventilator-associated pneumonia (VAP) caused by carbapenem-resistant Acinetobacter baumannii (CRAB) are both associated with significant morbidity and mortality in daily clinical practice, as well as in a critical care setting. It is unclear whether colistin susceptible-only Acinetobacter baumannii (CSO AB) is a unique phenotype separate from or a subset of CRAB-associated pneumonia. The aim of this study is to investigate the prevalence of CSO AB pneumonia and compare the presentation and outcome between CSO AB and CRAB-associated pneumonia in critically ill patients. This multicenter retrospective cohort study initially recruited 955 patients with CR-GNB pneumonia. After exclusion, 575 patients left who were ICU-admitted and had CRAB nosocomial pneumonia remained. Among them, 79 patients had CSO AB pneumonia, classified as the CSO AB group. The other 496 patients were classified as the CRAB group. We compared demographic characteristics, disease severity, and treatment outcomes between the two groups. The prevalence of CSO AB among all cases of CRAB pneumonia was 13.74% (79/575). The CSO AB and CRAB groups had similar demographic characteristics and disease severities at initial presentation. The in-hospital mortality rate was 45.6% and 46.4% for CSO AB and CRAB groups, respectively (p = 0.991). The CSO AB group had significantly better clinical outcomes at day 7 (65.8% vs 52.4%, p = 0.036) but longer length of ICU stay (27 days vs 19 days, p = 0.043) compared to the CRAB group. However, other treatment outcomes, including clinical outcomes at day 14 and 28, mortality, microbiological eradication, ventilator weaning, and newly onset dialysis, were similar. In conclusion, CSO AB accounted for 13.74% of all cases of CRAB pneumonia, and the clinical presentation and treatment outcomes of CSO AB and CRAB pneumonia were similar.

Differences in the Clinical Characteristics and 1-Year Mortality Rates of Patients with Dermatomyositis with anti-Jo-1 and anti-MDA5 Antibodies.

Patients with anti-Jo-1 antibodies (Abs) and anti-melanoma differentiation-associated protein 5 (MDA5) Abs are at a higher risk of interstitial lung disease (ILD) and have a mortality rate higher than that of patients with anti-Jo-1 Abs. This study investigated differences in the clinical characteristics and prognosis of patients with anti-Jo-1 Abs and anti-MDA5 Abs with dermatomyositis (DM). We retrospectively reviewed the medical records of 38 patients with DM from January 2000 to December 2021. The patients were divided into anti-Jo-1 Abs and anti-MDA5 Abs groups. The basic demographic data, clinical manifestations, and 1-year mortality rates of the groups were compared. Among the 38 patients, 30 were anti-Jo-1-Abs positive and 8 patients were anti-MDA5 Aba positive. The patients with anti-MDA5 Abs presented with more apparent cutaneous symptoms and aggressive pulmonary manifestations than did those with anti-Jo-1 Abs. The mortality rate in the anti-MDA5 Abs group (1.95/person-year (PY)) was much higher than that in anti-Jo-1 Abs group (0.094/PY), and most of the mortalities occurred within the first 1-3 months of follow-up. Distinct cutaneous and pulmonary manifestations were observed in the anti-Jo-1 Abs and anti-MDA5 Abs groups. The mortality rate in the anti-MDA5 Abs group was significantly higher than that in the anti-Jo-1 Abs group. Early recognition is crucial to ensuring higher chances of survival for patients with anti-MDA5 Abs.

The Value of Neoadjuvant Anthracycline-Based Regimens for HER2-Positive Breast Cancer: A Systematic Review and Meta-analysis Including 1366 Patients.

The standard recommendation for neoadjuvant therapy for human epidermal growth factor receptor-2 (HER2)-positive breast cancer patients is trastuzumab in combination with chemotherapy, but there is no current standard recommendation for appropriate chemotherapy regimens. This meta-analysis evaluated the efficacy and cardiac safety of the concurrent use of anti-HER2 targeted drugs and anthracycline-based neoadjuvant chemotherapy (NAC) for HER2-positive breast cancers. The pooled odds ratio (OR) rate for pathologic complete response (pCR), the pooled hazard ratio (HR) of overall survival (OS), and the left ventricular ejection fraction (LVEF) decline events were all calculated. Differences in efficacy, prognosis, and cardiac safety were compared between patients receiving an anthracycline-containing regimen (AB) and those treated with non-anthracycline-based (nAB) NAC. A total of 1366 patients in 4 prospective and 3 retrospective studies were included in the meta-analysis. The pooled OR for pCR rate was 0.73 with a 95% confidence interval (CI) of 0.43 to 1.24 (P = .246). Subgroup analysis of low tumor burden cases showed no improvement in pCR rate for patients in the AB group compared with nAB, with the pooled OR rate being 0.73 with a 95% CI of 0.37 to 1.44 (P= .357). The 3-year OS rate was 95.63% and 95.54% in the AB and nAB groups, respectively, with no statistical difference (P= .157). There was a significant increase in the rate of LVEF decline of 19.07% in the AB group compared with 13.33% for the nAB group, with an HR of 1.62 and a 95% CI of 1.11 to 2.36 (P = .013). The addition of anthracyclines did not improve pCR rates and survival after neoadjuvant and the increased cardiotoxicity of anthracyclines further limited their application. This study showed that it was feasible to use anti-HER2 drugs without anthracyclines in neoadjuvant therapy for HER2-positive breast cancer patients.

A study of A2B subgroup frequency among AB blood group in our tertiary care center

Background: The ABO blood group system invented by Karl Landstiner is popular blood grouping system and considered as the most vitalin blood transfusion safety. Approximately, 20% of individuals having A2 subgroup and 80% have A1 subgroup. Aims and Objectives: The objective of the present study is to find the prevalence of A1B and A2B subgroups in samples of patients in blood center of our tertiary care center. Materials and Methods: This observational study was carried out at blood center of tertiary care hospital. Blood group is separated into A, B, AB, and O-positive/negative. Based on serological reactivity, blood group was classified into A2B positive/A2B negative. Results: ABO blood grouping was analyzed for 5435 samples. Of these, 430 (7.91%) belonged to Group AB. Out of 430 AB group, 13 (3.1%) were having A2B-positive blood group and 417 (96.9%) were having A1B-positive blood group. Conclusion: Among AB blood group, frequency of A1B subgroup is higher than A2B. The study of this subgrouping can make blood transfusion more safe and can improve overall practice of transfusion.

Treatment of pancreatic cancer with irreversible electroporation and intratumoral CD40 antibody stimulates systemic immune responses that inhibit liver metastasis in an orthotopic model

BackgroundPancreatic cancer (PC) has a poor prognosis, and most patients present with either locally advanced or distant metastatic disease. Irreversible electroporation (IRE) is a non-thermal method of ablation used clinically...

Differential properties of Early vs. Late 18F‐Florbetaben imaging acquisition time frames

AbstractBackground18F‐Florbetaben (FBB) is a PET radioligand approved for determining binary amyloid status. According to the product label, FBB images may be acquired anytime 45‐130 minutes post injection. Protocols vary in the acquisition of FBB with respect to use of Early (50‐70 minutes‐post‐injection) or Late (90‐110 minutes‐post‐injection) frames. Identifying differences between Early and Late acquisitions of FBB data could aid in image interpretation and inform efforts towards harmonization across cohorts. We evaluated inter‐rater agreement of visual reads (VR) and differences in SUVR thresholds to characterize Early vs. Late frames.MethodFifty‐three subjects were studied including 23 patients meeting clinical criteria for amnestic mild cognitive impairment or mild Alzheimer’s disease and 30 cognitively normal controls. FBB images were acquired 50‐110 min post‐injection in 5‐min frames. Images were corrected for subject motion and separated into 50‐70 and 90‐110 min time windows. Three certified readers blind to cognitive status and time window provided a binary VR for each of the 106 data sets. Global standardized uptake value ratios (SUVRs) were calculated using a cerebellar gray matter reference. An ROC‐derived SUVR cut‐off value was determined for each window using a preliminary cohort (n=23) and evaluated in a larger cohort (n = 53).ResultInter‐reader agreement was similar in 50‐70 min and 90‐110 min scans, (Fleiss Kappa= 0.597 and 0.598). SUVRs from 50‐70 min and 90‐110 min scans were highly correlated (r = 0.99, p<0.0001). SUVRs were greater in visually positive than in visually negative scans for both time windows (Fig. 1; p<0.0001). SUVRs were still increasing at 50‐70 min but became but plateaued at 90 min. ROC‐derived SUVR cut‐off values (Fig. 1; SUVR50‐70=1.342, SUVR90‐110=1.524) largely agreed with the majority consensus VR (49/53; 92.5%) across both time frames.ConclusionEarly and Late frame data were highly correlated, and both provided robust quantitative differences between visually positive and negative Ab groups. However, imaging at 90‐110 min provides more stable SUVRs and therefore is preferred for measuring longitudinal change in amyloid burden.

Differential properties of Early vs. Late 18F‐Florbetaben imaging acquisition time frames

AbstractBackground18F‐Florbetaben (FBB) is a PET radioligand approved for determining binary amyloid status. According to the product label, FBB images may be acquired anytime 45‐130 minutes post injection. Protocols vary in the acquisition of FBB with respect to use of Early (50‐70 minutes‐post‐injection) or Late (90‐110 minutes‐post‐injection) frames. Identifying differences between Early and Late acquisitions of FBB data could aid in image interpretation and inform efforts towards harmonization across cohorts. We evaluated inter‐rater agreement of visual reads (VR) and differences in SUVR thresholds to characterize Early vs. Late frames.MethodFifty‐three subjects were studied including 23 patients meeting clinical criteria for amnestic mild cognitive impairment or mild Alzheimer’s disease and 30 cognitively normal controls. FBB images were acquired 50‐110 min post‐injection in 5‐min frames. Images were corrected for subject motion and separated into 50‐70 and 90‐110 min time windows. Three certified readers blind to cognitive status and time window provided a binary VR for each of the 106 data sets. Global standardized uptake value ratios (SUVRs) were calculated using a cerebellar gray matter reference. An ROC‐derived SUVR cut‐off value was determined for each window using a preliminary cohort (n=23) and evaluated in a larger cohort (n = 53).ResultInter‐reader agreement was similar in 50‐70 min and 90‐110 min scans, (Fleiss Kappa= 0.597 and 0.598). SUVRs from 50‐70 min and 90‐110 min scans were highly correlated (r = 0.99, p<0.0001). SUVRs were greater in visually positive than in visually negative scans for both time windows (Fig. 1; p<0.0001). SUVRs were still increasing at 50‐70 min but became but plateaued at 90 min. ROC‐derived SUVR cut‐off values (Fig. 1; SUVR50‐70=1.342, SUVR90‐110=1.524) largely agreed with the majority consensus VR (49/53; 92.5%) across both time frames.ConclusionEarly and Late frame data were highly correlated, and both provided robust quantitative differences between visually positive and negative Ab groups. However, imaging at 90‐110 min provides more stable SUVRs and therefore is preferred for measuring longitudinal change in amyloid burden.

Changes in the frequency of ABO and Rh blood group phenotypes in Polish donors from Lower Silesia

Abstract The aim of this study was to explore temporal and spatial variability of the frequency of the ABO and Rhesus blood group phenotypes in Polish donors from Lower Silesia between 1946 and 1990. Data on 158,134 individuals (113,714 men and 44,420 women) were obtained from two databases containing information about blood donors from Lower Silesia. The rate and direction of changes in the frequency of the blood groups were evaluated with regression analysis and Pearson’s correlation coefficient. Significant differences in the frequencies were found for the analysed period. The largest differences were observed for A and O blood groups. A significant increase in the frequency of the A group and a decrease in the O group were noted. The incidence of the AB group in both sexes did not change significantly. However, an insignificant decreasing trend in the frequency of the AB group in men was observed. An opposite and significant trend for this blood type was found in women. For the B group, the increase was significant in both sexes but it was greater in men. A significant increase in the frequency of the Rh(+) factor was noted. These results suggest that there were minor differences in the frequencies of ABO and Rh blood groups between the counties. This study demonstrated microevolutionary changes, thereby contributing new information to the field. These findings can be useful for the management of blood banks and the interpretation of microevolutionary changes.

Do Blood Phenotypes of Feline AB Blood Group System Affect the SARS-CoV-2 Antibody Serostatus in Cats?

Cats are susceptible to coronavirus infections, including infection by human severe acute respiratory syndrome coronavirus (SARS-CoV). In human ABO system blood groups, alloantibodies can play a direct role in resistance to infectious diseases. Individuals with the AB blood type were over-represented in the SARS-CoV-2 infection group. Blood type AB individuals lack both anti-A and anti-B antibodies, and therefore lack the protective effect against SARS-CoV-2 infection given by these antibodies. Starting from this knowledge, this pilot preliminary study evaluated a possible association between feline blood phenotypes A, B, and AB and serostatus for SARS-CoV-2 antibodies in cats. We also investigated selected risk or protective factors associated with seropositivity for this coronavirus. A feline population of 215 cats was analysed for AB group system blood phenotypes and antibodies against the nucleocapsid (N-protein) SARS-CoV-2 antigen using a double antigen ELISA. SARS-CoV-2 seropositive samples were confirmed using a surrogate virus neutralization test (sVNT). Origin (stray colony/shelter/owned cat), breed (DSH/non DSH), gender (male/female), reproductive status (neutered/intact), age class (kitten/young adult/mature adult/senior), retroviruses status (seropositive/seronegative), and blood phenotype (A, B, and AB) were evaluated as protective or risk factors for SARS-CoV-2 seropositivity. Seropositivity for antibodies against the SARS-CoV-2 N-protein was recorded in eight cats, but only four of these tested positive with sVNT. Of these four SARS-CoV-2 seropositive cats, three were blood phenotype A and one was phenotype AB. Young adult age (1-6 years), FeLV seropositivity and blood type AB were significantly associated with SARS-CoV-2 seropositivity according to a univariate analysis, but only blood type AB (p = 0.0344, OR = 15.4, 95%CI: 1.22-194.39) and FeLV seropositivity (p = 0.0444, OR = 13.2, 95%CI: 1.06-163.63) were significant associated risk factors according to a logistic regression. Blood phenotype AB might be associated with seropositivity for SARS-CoV-2 antibodies. This could be due, as in people, to the protective effect of naturally occurring alloantibodies to blood type antigens which are lacking in type AB cats. The results of this pilot study should be considered very preliminary, and we suggest the need for further research to assess this potential relationship.

Effect of moderate beer consumption (with and without ethanol) on osteoporosis in early postmenopausal women: Results of a pilot parallel clinical trial.

Osteoporosis is a chronic progressive bone disease characterized by low bone mineral density (BMD) and micro-architectural deterioration of bone tissue, leading to an increase in bone fragility and the risk of fractures. A well-known risk factor for bone loss is postmenopausal status. Beer may have a protective effect against osteoporosis associated with its content of silicon, polyphenols, iso-α-acids and ethanol, and its moderate consumption may therefore help to reduce bone loss in postmenopausal women. Accordingly, a 2-year controlled clinical intervention study was conducted to evaluate if a moderate daily intake of beer with (AB) or without alcohol (NAB) could have beneficial effects on bone tissue. A total of 31 postmenopausal women were assigned to three study groups: 15 were administered AB (330 mL/day) and six, NAB (660 mL/day), whereas, the 10 in the control group refrained from consuming alcohol, NAB, and hop-related products. At baseline and subsequent assessment visits, samples of plasma and urine were taken to analyze biochemical parameters, and data on medical history, diet, and exercise were collected. BMD and the trabecular bone score (TBS) were determined by dual-energy X-ray absorptiometry. Markers of bone formation (bone alkaline phosphatase [BAP] and N-propeptide of type I collagen [PINP]) and bone resorption (N-telopeptide of type I collagen [NTX] and C-telopeptide of type I collagen [CTX]) were determined annually. Bone formation markers had increased in the AB and NAB groups compared to the control after the 2-year intervention. However, the evolution of BMD and TBS did not differ among the three groups throughout the study period. Therefore, according to the findings of this pilot study, moderate beer intake does not seem to have a protective effect against bone loss in early post-menopausal women.

Construction of low intestinal bacteria model and its effect on laying performance and immune function of laying hens

The objective of this study was to establish a low-bacteria intestinal model in chickens, and then to investigate the characteristics involving in immune function and intestinal environment of this model. A total of 180 twenty-one-week-old Hy-line gray layers were randomly allocated into 2 treatment groups. Hens were fed with a basic diet (Control), or an antibiotic combination diet (ABS) for 5 weeks. Results showed that the total bacteria in the ileal chyme were significantly dropped after ABS treatment. Compared with the Control group, the genus-level bacteria such as Romboutsia, Enterococcus, and Aeriscardovia were reduced in the ileal chyme of the ABS group (P < 0.05). In addition, the relative abundance of Lactobacillus_delbrueckii, Lactobacillus_aviarius, Lactobacillus_gasseri, and Lactobacillus_agilis in the ileal chyme were also descended (P < 0.05). However, Lactobacillus_coleohominis, Lactobacillus_salivarius, and Lolium_perenne were elevated in the ABS group (P < 0.05). Beyond that, ABS treatment decreased the levels of interleukin-10 (IL-10) and β-defensin 1 in the serum, as well as the number of goblet cells in the ileal villi (P < 0.05). Additionally, the genes mRNA levels of the ileum such as Mucin2, Toll-like receptors 4 (TLR4), Myeloid differentiation factor 88 (MYD88), NF-κB, IL-1β, Interferon-gama (IFN-γ), IL-4 and the ratio of IFN-γ to IL-4 were also down-regulated in the ABS group (P < 0.05). In addition, there were no significant changes about egg production rate and egg quality in the ABS group. In conclusion, dietary supplemental antibiotic combination for 5 weeks could establish a low intestinal bacteria model of hens. The establishment of a low intestinal bacteria model did not affect the egg-laying performance, while caused immune suppression in laying hens.

Investigation on Circulating Neonatal Nav1.5 (nNav1.5) and Its Antibodies in the Blood and Serum of 4T1 Orthotopic Mice Model and Breast Cancer Patients from Hospital Universiti Sains Malaysia (HUSM)

Investigation on Circulating Neonatal Nav1.5 (nNav1.5) and Its Antibodies in the Blood and Serum of 4T1 Orthotopic Mice Model and Breast Cancer Patients from Hospital Universiti Sains Malaysia (HUSM)

ODP476 Does Levothyroxine Intake at Breakfast Matter for Patients With Hypothyroidism? A Meta- Analysis of Randomized Control Trials

Abstract The American Thyroid Association recommends taking levothyroxine consistently, "either 60 minutes before breakfast or at bedtime." However, clinicians typically opt to recommend taking levothyroxine on an empty stomach, rather than offering the option of bedtime or with breakfast. Many patients find it cumbersome to take levothyroxine in the morning without food. A meta-analysis was performed to evaluate the effects of taking levothyroxine (LT4) before breakfast (BB) vs. with breakfast (AB) on thyroid function tests in our included four clinical trials. Four randomized controlled trials involving 243 patients were retrieved from PubMed, according to established inclusion criteria. The Standard Mean Difference (SMD) of thyroid-stimulating hormone (TSH), free T4 (FT4), and free or total T3 (F/TT3) with their 95% confidence intervals between AB vs. BB were calculated. Random effect or fixed-effect model was used based on heterogeneity significance. Then a subgroup analysis of liquid levothyroxine and tablet levothyroxine was performed. Sensitivity analysis and publication bias detection were performed. All statistical analysis was performed using RevMan software (v5.4; Cochrane library) and R Core Team (2016, Vienna, Austria), and all p-values were two-tailed, and the significance level was 0. 05. No statistically significant differences of TSH (Mean 1.45-3.74 vs. 1.26-3. 00, SMD 0.25; 95% CI: -0. 06, 0.56; p=0.11, I2=65%), FT4 (Mean 1. 03-1.35 vs. 1. 02-1.24, SMD 0.14; 95% CI: -0.33,0.62; p=0.55, I2=78%), and F/TT3 (SMD -0. 06; 95% CI: -0.28,0.15; p=0.57, I2=0%) between AB vs. BB were found. The subgroup analysis showed that no statistically significant difference of TSH between AB vs. BB when patient took liquid form of levothyroxine (1.45-2.59 vs. 1.26–3. 00; SMD=0. 03; 95% CI: -0.21, 0.27; p=0.81, I2=0%). Although TSH was significantly higher with AB vs. BB when patient took tablet form of levothyroxine (2.89-3.74 vs. 1.54 -1.9; SMD=0.64; 95% CI: 0.24, 0.103; p=0. 001, I2=49%), TSH was within normal limits in both groups. This meta-analysis demonstrates that TSH of patients taking levothyroxine with breakfast is non-inferior to those taking it before breakfast. Although AB group taking levothyroxine by tablet has a significantly higher TSH level than the BB group, both groups have well-controlled levels. Limitations of this study include limited sample size and number of studies. It also was unable to differentiate patients with hypothyroidism from benign or malignant diseases. We also were not able to assess adherence to the prescribed regimen. Patient satisfaction and adherence with treatment may be improved by letting patients know to take LT4 consistently at a time of their choice. Presentation: No date and time listed

Supplementation of Paraformic Acid as a Substitute for Antibiotics in the Diet Improves Growth Performance and Liver Health in Broiler Chickens.

Simple SummaryParaformic acid (PFA) is a hyperpolymer formed by polymerization of formic acid monomers, and PFA has a lower pungent smell and corrosiveness to the gastrointestinal tract compared with formic acid. The liver is a crucial metabolic organ, playing a momentous role in nutrients biosynthesis and metabolism, defense against bacterial invasion, and endotoxin clearance. However, in the condition of intensive poultry husbandry, the broiler’s liver is susceptible to inflammatory damage. Our results showed that PFA supplemented to the diet improved growth performance, inhibited inflammatory response, and benefited liver protection, which provided a direction for the use of PFA in broiler production.The current study aimed to explore the effects of supplementing paraformic acid (PFA) into broilers’ diet on growth performance, inflammatory responses, and liver protection. A total of 567 healthy one-day-old broilers were used in a 42-d study, and they were randomized into three groups. Broilers were fed a basal diet (CON group) or the basal diet supplemented with either 50 mg/kg aureomycin (AB group) or 1000 mg/kg PFA (PFA group). The results showed that the PFA and AB groups had a higher feed conversion rate than the CON group from day 21 to 42 (p < 0.05). Dietary PFA or aureomycin supplementation decreased serum levels of interleukin (IL)-1β, IL-6, IL-10, alanine transaminase, diamine oxidase, and D-lactate, and significantly increased serum concentrations of immunoglobulin (Ig) A, IgM, and complement C4 (p < 0.05). Moreover, dietary PFA or aureomycin supplementation decreased hepatic levels of caspase-1, NOD-like receptor family pyrin domain containing 3 (NLRP3), tumor necrosis factor-alpha, IL-6, and IL-18, as well as NF-κB mRNA expression (p < 0.05). Above all, PFA supplementation into the broilers’ diet improved growth performance, inhibited inflammatory responses, and benefited liver protection. The protective effects of PFA on the liver might be related to inhibition of caspase-1-induced pyroptosis via inactivating the NF-κB/NLRP3 inflammasome axis in broiler chickens.

Effects of dietary Bopu powder supplementation on intestinal development and microbiota in broiler chickens.

This study aimed to investigate the effect of dietary supplementation with Bopu powder on intestinal development and bacterial community composition in broiler chickens. A total of 486 1-day-old arbor acres broilers were fed a basal diet (CON group), a basal diet supplemented with 50 mg/kg aureomycin (AB group), or a basal diet supplemented with 40 mg/kg Bopu powder (BP group). The results showed that the BP group had significantly lower serum tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and diamine oxidase concentrations and had significantly higher serum IL-10 concentrations than CON group (p < 0.05). Groups AB and BP had a significantly higher weight per unit length of the small intestine and villus height than the CON group (p < 0.05), and BP group had a significantly higher ratio of villus height to crypt depth than groups CON and AB (p < 0.05). Compared to the CON group, dietary Bopu powder or aureomycin supplementation significantly increased transforming growth factor-α concentration and mRNA expressions of zonula occludens-1 (ZO-1) and occludin, and decreased intestinal mucosal concentrations of TNF-α, IL-6, IL-10, caspase-3, and caspase-8 and mRNA expressions of nuclear factor-kappa-B and Bax/Bcl-2 ratio in the intestinal mucosa (p < 0.05). Meanwhile, BP group had significantly higher ZO-1, secretory immunoglobulin A, interferon-γ concentrations, and mRNA expressions of glucose transporter type-2 and sirtuin-1, and significantly lower IL-1β concentration than groups CON and AB in intestinal mucosa (p < 0.05). Dietary Bopu powder supplementation significantly increased the concentration of trefoil factor family member and mRNA expressions of superoxide dismutase-1 and bcl-2 associated X, and significantly reduced casepase-9 concentration and myeloid differentiation primary response-88 expression in the intestinal mucosa of broiler chickens relative to CON group (p < 0.05). Moreover, results of high-throughput sequencing showed that broilers in the BP group had microbial community structure distinct from that in CON group, and the addition of Bopu powder increased the abundances of Faecalibacterium and Colidextribacter (p < 0.05). Therefore, our study suggests a synergic response of intestinal development and microbiota to the Bopu powder, and provides a theoretical basis as a potential substitute for antibiotics.