Higher Order Thinking Skills (HOTS) has become the focus of learning mathematics at all levels, especially for prospective math student-teachers because their abilities are not limited to themselves only. However, when they become teachers in the future, they have a tough task of making students to have HOTS. The study aimed to analyze HOTS of students studying mathematics in Matrix subject. The research method used was qualitative descriptive that analyzed and described students’ midterm exam answers based on indicators of HOTS to analyze (C4), evaluate (C5), and create (C6). The research subjects were Mathematics students of teacher training education at Lambung Mangkurat University that were taking Matrix subjects in 2018/2019 even semester involved in the Matrix lecture process. The result of the study was concluded that the thinking skills of students in the Matrix subjects in faculty of teacher training education, Lambung Mangkurat University, were in sufficient category. The results of the study should be a reference for the lecture process where students are familiarized with giving HOTS-oriented questions during exams and in lecture practice to help in developing them.