Effects Of Aberrations Research Articles

Axial resolution improvement of two-photon microscopy by multi-frame reconstruction and adaptive optics.

Two-photon microscopy (TPM) has been widely used in biological imaging owing to its intrinsic optical sectioning and deep penetration abilities. However, the conventional TPM suffers from poor axial resolution, which makes it difficult to recognize some three-dimensional fine features. We present multi-frame reconstruction two-photon microscopy (MR-TPM) using a liquid lens as a fast axial scanning engine. A sensorless adaptive optics (AO) approach is adopted to correct the aberrations caused by both the liquid lens and the optical system. By overcoming the effect of optical aberrations, inadequate sampling, and poor focusing capability of a conventional TPM, the axial resolution can be improved by a factor of 3 with a high signal-to-noise ratio. The proposed technology is compatible with the conventional TPM and requires no optical post-processing. We demonstrate the proposed method by imaging fluorescent beads, in vitro imaging of the neural circuit of mouse brain slice, and in vivo time-lapse imaging of the morphological changes of microglial cells in septic mice model. The results suggest that the axon of the neural circuit and the process of microglia along the axial direction, which cannot be resolved using conventional TPM, become distinguishable using the proposed AO MR-TPM.

Effects of aberrations on the point spread function and the two-point resolution with versatile apodization pupils

In this work, efficient amplitude-apodization pupils have been presented for tailoring the point spread function (PSF), which results in a decreased full-width at half-maximum (FWHM) and suppressed sidelobes. Comparison of PSF intensity profiles for both the unapodized and apodized cases have been reported. By analysing the resulting PSFs, the well-known effects induced by monochromatic aberrations such as defocussing effect, and the primary spherical aberration have been controlled for various degrees of apodization parameter $$\beta $$ . It is shown that the two-point resolution of the apodized optical system significantly increased in the presence of aberrations. The proposed pupil apodizer is very effective in enhancing the resolution of the optical systems applicable in imaging and focussing applications.

Dark field proton radiography

A pre- and post-collimation scheme has been applied to high energy proton radiography to establish a dark field condition, which defaults to a state of no transmission until a scatterer is placed at the object plane. This technique, dark field proton radiography, provides two additional capabilities to a standard proton radiography setup. First, protons with a high degree of angular dispersion are removed from the beam, reducing the effects of chromatic aberrations and decreasing noise. Second, protons below the same threshold are removed from the beam downstream of the objects, effectively making the transmission highly sensitive to small amounts of scatter at the object plane. Initial results indicate that the system is highly sensitive to the presence of thinner materials and improves sensitivity to subtle areal density variations in thick objects.

Effects of aberration on super-resolution ultrasound imaging using microbubbles

Visualizating vasculature beyond the diffraction limit can be achieved using ultrasound super-resolution (USR). Typically, ultrasound (US) scanners model the target medium as homogeneous, assuming a constant speed-of-sound for time-of-flight based calculations. However, variations in US propagation velocity caused by varying tissue layers affect beamforming operations. This aberration is likely to have considerable effect on USR accuracy when imaging at depth. Here we investigate the effect of aberration on USR localization accuracy. Wave propagation through different tissue media was modelled from a linear transducer using k-Wave, and the resulting pulse-echo data from a point scatterer located at 45mm depth was beamformed. Media included a control homogeneous propagation medium, and aberrating media typical of liver imaging. Aberration effects were estimated using RMS arrival-time fluctuations (ATF) and energy-level fluctuations (ELF) at the scatterer location. Signals were extracted and localized with existing localization techniques. Results indicated USR localisation accuracy decreased with increasing aberration. Axial localisation errors reached 836.6 mm for an ATF 30.9 ns. Furthermore, errors increased with decreasing frequency from 4 to 3 MHz. The scale of these errors relative to micro-vascular structures of interest suggests that aberration will have considerable impact on USR performance and requires attention to ensure its success.

Aberrant effect of genistein on placenta development expressed through alteration in transforming growth factor-β1 and alkaline phosphatase across the maternal serum, the placenta and the amniotic fluid.

Objective(s):The mechanism via which genistein, the major isoflavone content of soya, adversely influenced placenta and fetal development was evaluated in pregnant laboratory rats.Materials and Methods:There were control, 2 mg/kg and 4 mg/kg genistein groups of rats with five sub-groups based on gestation termination day. At the end of the experiment, animals were sacrificed by CO2 and cervical dislocation, while plasma and serum were processed and stored. The abdomen was opened and the amniotic fluid was siphoned from the uterine sacs, processed and stored. The embryonic implants were excised, the placenta was separated from the fetus and weighed separately. Placenta homogenate was prepared from the harvested placenta, while the rest were processed for histological studies. Transforming growth factor (TGf-β1) and alkaline phosphatase (ALP) were assayed for in all samples. A significant decrease in the placenta and fetal weights, and a significant decrease in serum and placenta homogenate ALP levels were recorded in genistein groups.Results:There was a reduction in the Trophoblast giant cells population (TGCs). TGCs zone depth, perimeter, and an increase in the placenta and amniotic fluid’s TGf-β1 in all genistein groups at GD-13 towards term, and GD-18 and GD-20, respectively. Maternal plasma TGf-β1 was increased in 2 mg group early in pregnancy while its level significantly decreased in both 2 mg and 4 mg genistein groups at mid-gestation towards GD-19. Conclusion:Genistein aberrant effect on fetal development was via its adverse effect on TGCs proliferation and TGf-β1 activities in the placenta tissue.

A brief discussion of the benefit and mechanism of vitamin D supplementation on coronavirus disease 2019.

Vitamin D exerts extraskeletal functions, including immunomodulatory activity, protection against respiratory tract infections and pleiotropic effects on the cardiovascular system. Since the outbreak of the coronavirus disease-2019 (COVID-19) pandemic, several articles have suggested the potential involvement of vitamin D in reducing the risk and severity of the disease. Epidemiological and observational studies support the hypothesis of a protective role of vitamin D but most studies are retrospective or based on small samples. However, the pandemic progression and the increased knowledge on the pathogenesis of COVID-19 have challenged the first evidence, suggesting also potential negative consequences derived by adequate vitamin D status. A cautious interpretation of the significance of low vitamin D25OH levels is advisable. The balance between over-activation of innate immunity and the exhaustibility of the adaptive immune response still needs to be clarified. In addition, the modulation of endothelial function, the down-regulation of renin, angiotensin-converting-enzyme (ACE) and angiotensin genes and the up-regulation of ACE2 expression is still an area of research. Speculative hypotheses and observational data have suggested a protective role of vitamin D in COVID-19. However, many unanswered questions remain, aberrant detrimental effects of adequate vitamin D25OH levels cannot be excluded and whether its adequacy may prevent the infection or improve clinical outcomes needs to be assessed by adequately sized and designed population-based studies and intervention trials.

Residual molecular and behavioral effects of the phenylpyrazole pesticide fipronil in larval zebrafish (Danio rerio) following a pulse embryonic exposure

Pesticides are typically applied to crops as acute applications, and residual effects of such intermittent exposures are not often characterized in developing fish. Fipronil is an agricultural pesticide that inhibits γ-amino-butyric acid (GABA) gated chloride channels. In this study, zebrafish (Danio rerio) embryos were exposed for 48 h (starting at ~3 h post fertilization, hpf) to various concentrations of fipronil (0.02 μg/L up to 4000 μg/L). Following this acute exposure, a subset of fish was transferred to clean water for a 7-day depuration phase. We hypothesized that a pulse exposure to fipronil during critical periods of central nervous system development would adversely affect fish later in life. After a 48 hour pulse exposure, survival was reduced in embryos exposed to 2 μg fipronil/L or greater. However, there was no further mortality during the depuration phase, nor were there changes in body length nor notochord length in larvae 9 dpf (days post-fertilization) compared to controls. Additional experiments were carried out at higher concentrations over 96 h (up to 4 dpf) to also elucidate developmental effects and teratogenicity of fipronil (43.7 μg/L up to 4370 μg/L). Fipronil at these higher concentrations significantly impacted the development of zebrafish, and the following morphometric and teratogenic effects were observed in 4 dpf fish; reduced body length, yolk sac and pericardial edema, reduced midbrain length, reduced optic and otic diameter, and truncation of the lower jaw. In depurated fish, we hypothesized that there would exist residual effects of exposure at the molecular level. Transcriptome profiling was therefore conducted on 9 dpf depurated larvae exposed initially for 48 h to one dose of either 0.2 μg/L, 200 μg/L or 2000 μg/L fipronil. The expression of gene networks associated with glycogen and omega-3-fatty acid metabolism were decreased in larvae exposed to each of the three concentrations of fipronil, suggesting metabolic disruption. Moreover, transcriptomics revealed that fipronil suppressed gene networks related to light-dark adaptation, photoperiod sensing, and circadian rhythm. Based on these data, we tested fish for altered behavioral responses in a Light-Dark preference test. Larvae exposed to >200 μg fipronil/L as embryos showed fewer number of visits (20–30% less) to the dark zone compared to controls. Larvae also spent a lower amount of time in the dark zone compared to controls, suggesting that fipronil strengthened dark avoidance behavior which is indicative of anxiety. This study demonstrates that a short pulse exposure to fipronil can affect transcriptome networks for metabolism, circadian rhythm, and response to light in fish after depuration, and these molecular responses are hypothesized to be related to aberrant behavioral effects observed in the light-dark preference test.

A role of color vision in emmetropization in C57BL/6J mice

Spectral composition affects emmetropization in both humans and animal models. Because color vision interacts the effects of chromatic defocus, we developed a method to bypass the effects of longitudinal chromatic aberration by placing a spectral filter behind the optics of the eye, using genetic tools. Newborn C57BL/6J (B6) mice were reared in quasi-monochromatic red (585–660 nm) or blue (410–510 nm) light beginning before eye-opening. Refractive states and ocular dimensions were compared at 4, 6, 8, and 10 weeks with mice reared in normal white light. Cre recombinase-dependent Ai9 reporter mice were crossed with Chx10-Cre to obtain Chx10-Cre;Ai9 mice, expressing red fluorescent protein in retinal Cre-positive cells. Ai9 offsprings, with and without Cre, were reared under a normal visual environment. Refraction and axial components were measured as described above. Expression levels of M and S opsin were quantified by western blotting at 10 weeks. Compared with those reared in white light, B6 mice reared in red light developed relative hyperopia, principally characterized by flattening of corneal curvature. Emmetropization was not affected by blue light, possibly because the reduction in vitreous chamber depth compensated for the increase in corneal curvature. Compared with Cre-negative littermates, the refraction and axial dimensions of Chx10-Cre;Ai9 mice were not significantly different at the follow-up timepoints. M opsin levels were higher in Chx10-Cre;Ai9 mice at 10 weeks while S opsin levels were not different. Red light induced a hyperopic shift in mouse refractive development. Emmetropization was not impacted in mice with perturbed color vision caused by intrinsic red-fluorescent protein, suggesting that color vision may not be necessary in mouse emmetropization when other mechanisms are present.

The Effect of Visual Aberration on Burn Dressing Replacement Pain in Children Aged 6-12 Years: A Clinical Trial Study

The Effect of Visual Aberration on Burn Dressing Replacement Pain in Children Aged 6-12 Years: A Clinical Trial Study

Generalization of the k coefficient method in relativity to an arbitrary angle between the velocity of an observer (source) and the direction of the light ray from (to) a faraway source (observer) at rest

The k coefficient method proposed by Bondi is extended to the general case where the angle α between the velocity of a signal from a distant source at rest and the velocity of the observer does not coincide with 0 or π, as considered by Bondi, but takes an arbitrary value in the interval 0 ⩽ α ⩽ π, and to the opposite case where the source is moving and the observer is at rest, while the angle α between the source velocity and the direction of the signal to the observer takes any value between 0 and π. Functions k*(β, α) and k+(β, α) of the angle and relative velocity are introduced for the ratio ω / ω′ of proper frequencies of the source and observer. Their explicit expressions are obtained without using Lorentz transformations, from the condition that the coherence of a bunch of rays is preserved in passing from the source frame to the observer frame. Owing to the analyticity of these functions in α, the ratio of frequencies in the cases mentioned is given by the formulas ω / ω′ = k*(β, α) and ω / ω′ = k+(β, π – α) ≡ 1 / k*(β, α), which coincide with those for the Doppler effect, in which the angle α, the velocity β, and one of the frequencies are measured in the rest frame. A ray emitted by the source at an angle α to the observer’s velocity in the source frame is directed at an angle α′ to the same velocity in the observer frame. Owing to light aberration, the angles α and α′ are functionally related through k*(β, α) = k+(β, β′). The functions α′(α, β) and α (α′,β) are expressed as antiderivatives of k*(β, α) and k*(β, π – α′). The analyticity of the functions k*(β, z) and k+(β, z) in z ≡ α in the interval 0 ⩽ z ⩽ π is extended to the entire plane of complex z, where k* has poles at i ln cos α1 (see ()), and k+ has zeros at the same points shifted by π. The spatiotemporal asymmetry of the Doppler and light aberration effects is explained by the closeness of these singularities to the real axis.

Вплив аберацій та ефектів розфокусування лазерного проектора на ефективність зменшення спеклових шумів

The impact of aberrations and the effect of defocusing on the efficiency of reduction of speckle noise in the system of a laser projector containing a moving diffractive optical element (DOE) is investigated. The effects of aberration and defocusing in the mechanisms of reducing speckle noise in laser projection displays were studied using the Fresnel approximation and the model of thin lenses. The results of the analysis based on the number of diffraction orders that have passed through the optical system are presented. The analysis showed that aberrations should not affect the contrast of the speckles, and the displacement of the screen relative to the image plane (defocus) only leads to re-scaling of the autocorrelation function of the scanning beam, which is equivalent to refocusing the lens to a new screen position. The use of the speckle-noise reduction method when scanning a laser display using Barker code should not require an increase in the resolution of the projection system screen compared to normal values. The resolution is determined exclusively by the requirements to image quality. It is shown that small aberrations change only the phase of the wave-front of the diffraction orders and therefore do not change the number of diffraction orders that illuminate a given area of the screen, and therefore do not change the contrast of the speckles. But larger aberrations cause a reversal of the direction of propagation of the rays that have passed through the lens. The contrast of speckles in the paraxial region of the screen is almost insensitive to aberration, but the contrast of speckles in peripheral areas changes abruptly if the aberrations change the number of diffraction orders that illuminate the area. In the optical circuit with DOE located in front of the optical modulator, the contrast of the speckles is not sensitive to the distance between the optical modulator and DOE, if the optical modulator is illuminated by all diffraction orders. Fig.: 4. Refs: 10 titles.

Propagation characteristics of a spatially chirped Gaussian laser beam propagating through multiple lenses

Variation of curvature of laser beam changes characteristics of spatially chirped pulsed Gaussian (SCPG) laser beam primarily due to varying spectral divergence. Parametric dependence of different pulse parameters, e.g. angular chirp, pulse front tilt, pulse width, beam radius, radius of curvature etc., is presented for a SCPG laser beam propagating through single and pair of reflective or refractive optical system. Effect of chromatic aberration on characteristic of SCPG laser beam have also been presented under different propagation conditions. Different expressions for various parameters of pulsed Gaussian beam are extended using recursive relation for a sequence of multiple chromatic lenses generally used in large scale high energy high power laser system. Discussion is supported with few examples of lens system.

Tilt-to-length noise coupled by wavefront errors in the interfering beams for the space measurement of gravitational waves.

The space-based gravitational wave detection programs, like the Laser Interferometer Space Antenna (LISA) or the Taiji program, aim to detect gravitational waves in space with a triangular constellation of three spacecraft. The unavoidable jitters of the spacecraft and the pointing will couple with the misalignment of the interfering beams into the longitudinal path length readout. This effect is called tilt-to-length (TTL) coupling, which is one of the keys to achieving the required measuring accuracy of 1pm/Hz. In terms of two phase definitions (the LISA Pathfinder (LPF) signal and the Average Phase (AP) signal), we implement the comprehensive theoretical analysis concerning the effect of aberrations on TTL coupling noise. In addition, we analytically derive that the proper lateral shift of the interfering beams relative to the detector can partly cancel out the TTL noise coupled with aberrations, especially coma and trefoil aberrations for the AP signal. Based on the above results, the meaningful guidance can be provided for the design and construction of the optical system in LISA or Taiji.

Abstract 5091: TIMP-1 in tumor-associated fibroblasts drives tumor progression in lung adenocarcinoma through CD63 interaction

Abstract Tumor associated fibroblasts (TAFs) are important regulators of tumor growth and resistance to therapies. We have recently shown that lung TAFs in vitro respond to the antifibrotic drug nintedanib in adenocarcinoma (ADC) but not squamous cell carcinoma (SCC). We also showed that the tumor-promoting effects of TAFs are driven by different mechanisms in ADC and SCC, which remain to be elucidated. Tissue inhibitor of metalloproteinases 1 (TIMP-1) has been associated with poor prognosis in lung cancer, its expression is downregulated by nintedanib, and our preliminary results reveal that its putative receptor, CD63, is overexpressed in ADC patients compared to SCC, supporting a selective crosstalk between TAFs and cancer cells in ADC through TIMP-1 and CD63. The aim of this study was to test this hypothesis using in vitro preclinical models. Primary fibroblasts were obtained with the patient informed consent, and using protocols approved by the Ethics Committee of the Hospital Clinic. ADC-TAFs and SCC-TAFs were stimulated with TGF-β1 in the presence or absence of nintedanib, and the TIMP-1 content in their conditioned medium was determined by ELISA. TIMP-1 was knocked-down in ADC-TAFs by siRNA, and the corresponding conditioned medium was used to stimulate growth and invasion of the high-CD63 ADC cell line, H1437. Likewise, CD63 expression in H1437 cells was reduced by siRNA. Our in vitro results showed that TIMP-1 secretion induced by TGF-β1 is significantly larger in ADC-TAFs compared to SCC-TAFs. Likewise, nintedanib elicited a higher downregulation of secreted TIMP-1 in ADC-TAFs compared to SCC-TAFs. Of note, TIMP-1 from ADC-TAFs was necessary to induce growth and invasion of H1437 cells. Likewise, knocking-down CD63 in H1437 ADC cells was sufficient to reduce the growth and invasion elicited by the conditioned medium of TGF-β1 activated ADC-TAFs. Collectively, our results unveil a novel stroma-carcinoma crosstalk driven by TIMP-1 and CD63 selectively in lung ADC, and support that such heterotypic crosstalk may underlie the aberrant tumor-promoting effects of ADC-TAFs that are selectively downregulated by nintedanib. Citation Format: Paula Duch, Marta Gabasa, Rafael Ikemori, Marselina Arshakyan, Frank Hillberg, Noemí Reguart, Derek Radisky, Jordi Alcaraz. TIMP-1 in tumor-associated fibroblasts drives tumor progression in lung adenocarcinoma through CD63 interaction [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 5091.

Analysis and modulation of aberration in an extreme ultraviolet lithography projector via rigorous simulation and a back propagation neural network.

Lens aberration is a critical factor affecting lithography, one that deteriorates the image fidelity and contrast. As the perfect lens does not exist, the aberration control is important for real optical systems, especially for extreme ultraviolet lithography (EUVL). By choosing the process variation band (PVB) and pattern shift (PS) as the lithographic performance indicators, the inverse analysis model for aberration control is proposed in this paper. First, the effects of aberration with 36 Zernike terms on lithography performance are forward analyzed. Using the definitive screening design (DSD) and with the help of statistical analysis methods of analysis of variance and F test, the combined Zernike terms leading to prominent PVB and PS are identified. After giving a brief introduction of backpropagation neural network (BPNN), the aberration control model based on DSD and BPNN is then established. Finally, several examples are analyzed to demonstrate the effectiveness and robustness of the aberration control model. Predicted results show that the optimum distribution of Zernike coefficients given by the aberration model can generate minimum impact on imaging quality, and this impact is very close to that of zero aberration. The results demonstrate that the BPNN-based aberration model has the potential to be an efficient guiding method for controlling the aberration of EUVL in the optical design stage.

Enhancing the Intermediate Vision of Monofocal Intraocular Lenses Using a Higher Order Aspheric Optic.

To describe and evaluate a new monofocal intraocular lens (IOL) designed to improve intermediate vision using a unique refractive technology. The new monofocal lens is based on a higher order aspheric optic and is designed to improve intermediate vision. Simulated visual acuity from far to -2.00 diopters (D) was calculated using optical bench data. The effect of corneal higher order aberrations (HOAs) on simulated visual acuity, pupil size, and decentration was assessed using realistic computer eye models. The susceptibility to photic phenomena was evaluated by measuring preclinically the intensity of the light distribution in the retinal plane. The new lens design was compared to a standard aspheric monofocal IOL that shares the same platform, material, and primary spherical aberration as the new design. Simulated defocus curves showed increased simulated visual acuity in the intermediate range compared to a standard aspheric monofocal IOL with comparable distance vision, independently of the pupil size and corneal HOAs. At -1.50 D, the new IOL design provided a gain of approximately 0.1 logMAR, whereas at distance, the difference was less than 0.05 logMAR. The tolerance to decentration was also similar in both designs. Finally, experimental results indicate that the susceptibility to photic phenomena with the new lens design was similar to that of a standard aspheric monofocal IOL. Preclinical data showed that the new lens design improves intermediate vision while maintaining comparable distance image quality and keeping the same photic phenomena profile as a standard aspheric monofocal IOL. [J Refract Surg. 2020;36(8):520-527.].

Lossless Monochromation for Electron Microscopy with Pulsed Photoemission Sources and Radio-Frequency Cavities

Resonant radiofrequency cavities enable exquisite time-energy control of electron beams when synchronized with laser driven photoemission. We present a lossless monochromator design that exploits this fine control in the one-electron-per-pulse regime. The theoretically achievable maximum beam current on target is orders of magnitude greater than state-of-the-art monochromators for the same spatial and energy resolution. This improvement is the result of monochromating in the time domain, unconstrained by the transverse brightness of the electron source. We show analytically and confirm numerically that cavity parameters chosen to minimize energy spread perform the additional function of undoing the appreciable effect of chromatic aberration in the upstream optics. We argue that our design has significant applications in both ultra-fast and non-time-resolved microscopy, provided photoelectron sources of sufficiently small size and laser sources of sufficiently high repetition rate. Our design achieves in simulations more than two orders of magnitude reduction in beam energy spread, down to single digit meV. Overcoming the minimum probe-size limit that chromatic aberration imposes, our design clears a path for high-current, high-resolution electron beam applications at primary energies from single to hundreds of keV.

Analytical expression of aperture efficiency affected by Seidel aberrations.

The effect of aberrations on the aperture efficiency has not been discussed analytically, though aberrations determine the performance of a wide field-of-view system. Expansion of a wavefront error and a feed pattern into a series of the Zernike polynomials enables us to calculate the aperture efficiency. We explicitly show the aperture efficiency affected by the Seidel aberrations and derive the conditions for reducing the effects of the spherical aberration and coma. In particular, the condition for coma can reduce a pointing error. We performed Physical Optics simulations and found that, if the Strehl ratio is higher than 0.8, the derived expression provides the aperture efficiencies with a precision of <2%.

Wavefront coding with Jacobi-Fourier phase masks for retinal imaging.

Wavefront coding is a technique that combines optical phase elements and digital signal processing in order to increase the effective depth of focus of optical systems. The success of wavefront coding lies in the design of a suitable phase mask placed at the system's aperture. This element allows for image formation invariant under the effects of different second-order optical aberrations. In optical systems limited by temporally or spatially varying high-order aberrations, the use of wavefront coding has not been fully demonstrated. Here we propose the choice of Jacobi-Fourier shaped phase masks to produce sharp and clear retinal images of living eyes. To demonstrate the potential use of the technique, we analyze the performance of the Jacobi-Fourier phase masks through experimental simulations to alleviate aberrations for different eye aberrations. We will show that the best mask choice is robust to noise while keeping acceptable resolution and reducing image artefacts.

Testing the effect of ocular aberrations in the perceived transverse chromatic aberration.

We have measured the ocular transverse chromatic aberration (TCA) in 11 subjects using 2D-two-color Vernier alignment, for two pupil diameters, in a polychromatic adaptive optics (AO) system. TCA measurements were performed for two pupil diameters: for a small pupil (2-mm), referred to as 'optical TCA' (oTCA), and for a large pupil (6-mm), referred to 'perceived TCA' (pTCA). Also, the TCA was measured through both natural aberrations (HOAs) and AO-corrected aberrations. Computer simulations of pTCA incorporated longitudinal chromatic aberration (LCA), the patient's HOAs measured with Hartmann-Shack, and the Stiles-Crawford effect (SCE), measured objectively by laser ray tracing. The oTCA and the simulated pTCA (no aberrations) were shifted nasally 1.20 arcmin and 1.40 arcmin respectively. The experimental pTCA (-0.27 arcmin horizontally and -0.62 vertically) was well predicted (81%) by simulations when both the individual HOAs and SCE were considered. Both HOAs and SCE interact with oTCA, reducing it in magnitude and changing its orientation. The results indicate that estimations of polychromatic image quality should incorporate patient's specific data of HOAs, LCA, TCA & SCE.