8-OHdG Levels Research Articles

Association between Polymorphisms of Hemochromatosis (HFE), Blood Lead (Pb) Levels, and DNA Oxidative Damage in Battery Workers.

Occupational exposure to lead (Pb) continues to be a serious public health concern and may pose an elevated risk of genetic oxidative damage. In Brazil, car battery manufacturing and recycling factories represent a great source of Pb contamination, and there are no guidelines on how to properly protect workers from exposure or to dispose the process wastes. Previous studies have shown that Pb body burden is associated with genetic polymorphisms, which consequently may influence the toxicity of the metal. The aim of this study was to assess the impact of Pb exposure on DNA oxidative damage, as well as the modulation of hemochromatosis (HFE) polymorphisms on Pb body burden, and the toxicity of Pb, through the analysis of 8-hydroxy-2'-deoxyguanosine (8-OHdG), in subjects occupationally exposed to the metal. Male Pb-exposed workers (n = 236) from car battery manufacturing and recycling factories in Brazil participated in the study. Blood and plasma lead levels (BLL and PLL, respectively) were determined by ICP-MS and urinary 8-OHdG levels were measured by LC-MS/MS, and genotyping of HFE SNPs (rs1799945, C → G; and 1800562, G → A) was performed by TaqMan assays. Our data showed that carriers of at least one variant allele for HFE rs1799945 (CG + GG) tended to have higher PLL than those with the non-variant genotype (β = 0.34; p = 0.043); further, PLL was significantly correlated with the levels of urinary 8-OHdG (β = 0.19; p = 0.0060), while workers that carry the variant genotype for HFE rs1800562 (A-allele) showed a prominent increase in 8-OHdG, as a function of PLL (β = 0.78; p = 0.046). Taken together, our data suggest that HFE polymorphisms may modulate the Pb body burden and, consequently, the oxidative DNA damage induced by the metal.

Effect of Supplementation with Black Chokeberry (Aronia melanocarpa) Extract on Inflammatory Status and Selected Markers of Iron Metabolism in Young Football Players: A Randomized Double-Blind Trial.

The use of herbal medicinal products and supplements in amateur and professional sports has increased in the last decades. This is because most of these products and supplements contain bioactive compounds with a variety of biological properties that exert a physiological effect on the human body. The aim of this study was to analyze the effect of dietary supplementation with lyophilized black chokeberry extract on the levels of pro-inflammatory cytokines, hepcidin, and selected markers of iron metabolism in a group of young football players. This double-blind study included 22 male football players (mean = 19.96 ± 0.56), divided into two groups: supplemented and placebo. Before and after a 90-day period of training combined with supplementation (6 g of lyophilized black chokeberry extract), participants performed maximal multistage 20-m shuttle run tests at the beginning and at the end of the supplementation period, with blood sampled for analysis at different times before and after exercise. The levels of IL-6, IL-10, ferritin, myoglobin, hepcidin, 8-OHdG, albumin, and TAC were analyzed. The analysis of variance revealed a significant effect of 90-day supplementation with the lyophilized extract on changes in the IL-6 and IL-10 levels, and TAC induced by maximal aerobic effort. In conclusion, supplementation with lyophilized black chokeberry extract improves the performance and antioxidant status of serum in humans and induces protective changes in inflammatory markers.

Association between atmospheric pollutant levels and oxidative stress in pregnant women and newborns in Urumqi

BackgroundFrequent heavy air pollution occurred during the winter heating season of northern China. Particulate air pollution is a serious concern in Urumqi during heating season. Exposure to air pollution is known to increase adverse health outcomes, particularly oxidative damage. This study aimed to evaluate the impact of air pollution on oxidative damage around pregnant women and newborns in Urumqi.MethodsThis prospective observational study enrolled pregnant women in the Fifth Affiliated Hospital of Xinjiang Medical University between January 2019 and October 2019. Pregnant women and newborns were allocated into a heating season group (January - end of April 2019, October 2019) or non-heating season group (June 2019 - end of September) according to the specific delivery time. Venous blood, urine from the women and cord blood from their newborns were collected to measure the levels of PAHs and 8-deoxyguanosine (8-OHdG), a measure of oxidative stress.ResultsA total of 200 pregnant women and newborns were enrolled, with 100 pregnant women and newborns in the heating season group. Compared to the non-heating season group, the total contents of 8-OHdG in maternal urine, PAHs and 8-OHdG in maternal plasma and neonatal cord blood were higher in the heating season group (all P < 0.001). The average values for AQI, PM2.5, PM10, SO2, NO2, and CO were higher in the heating season group (all P < 0.001). Maternal and neonatal PAHs were correlated with 8-OHdG measurements in maternal urine (r = 0.288, P < 0.001 and r = 0.336, P < 0.001) and neonatal umbilical cord blood (r = 0.296, P < 0.001 and r = 0.252, P < 0.001). There was also a positive relationship between PAHs, 8-OHdG levels in pregnant women and their newborns and proximate air pollutant concentrations (all P < 0.05). Based on the results of multiple linear regression analysis, it was found that air pollutants(PM10, 03) had a great influence on the level of 8-OHdG in neonatal cord blood, and the contribution rate was high(R2 = 0.320). Based on the epidemiological questionnaire, a multiple linear regression model was established(R2 = 0.496). We found that 8-OHdG levels in neonatal umbilical cord blood were mainly affected by two aspects: (1) Biological samples collected during heating had higher levels of 8-OHdG in neonatal umbilical cord blood. (2) Study may suggest that in neonates, males are more sensitive to oxidative damage.ConclusionParticulate air pollution may increase PAHs exposure and oxidative DNA damage in pregnant women and newborns.

Removal of lithium from aqueous solutions by solid-phase extraction using sawdust loaded with magnetite nanoparticles and study of apoptosis, MDA and 8-OHdG caused by lithium toxicity in fish brain.

Lithium, which has a high industrial value, is an environmental pollutant of concern to those who work with lithium in industry as well as to the general public. Biological parameters such as MDA, 8-OHdG, apoptosis (caspase-3), and acetylcholinesterase (AChE) were studied to determine the toxic effects on the brain tissue of the model organism (Carassius auratus) exposed to high dose lithium. According to the results obtained, it was found that lithium exposure caused oxidative stress with an increase in MDA level over time and, accordingly, DNA damage and apoptosis occured in brain tissue. It was also found that a decrease in AChE activity was observed, and the high levels of MDA, 8-OHdG, and caspase-3 activity obtained in brain tissue supported this result. The solid phase extraction (SPE) method was used to effectively remove lithium, which has unfavorable effects on living organisms, from aqueous solutions. In this method, a sawdust loaded with magnetite nanoparticles (MNLS) was prepared as an adsorbent for solid phase extraction by a simple method, and it was characterized. Optimal conditions for the SPE process were defined and it was found that lithium could be removed from solution onto the MNLS surface with a high yield of about 96%. The results of the study are crucial for proposing a simple and applicable high performance method.

Telomere Length and Telomerase Activity of Granulosa Cells and Follicular Fluid in Women Undergoing In Vitro Fertilization

This study aimed to evaluate the interrelationship between telomere length, telomerase activity and oxidative DNA damage in patients undergoing in vitro fertilization (IVF). This single-center, observational clinical study comprised 102 unselected, consecutive patients with various infertility diagnoses. Granulosa cells (GCs) and follicular fluid (FF) were analyzed simultaneously for telomere functions and for the marker of oxidative DNA damage, 8-hydroxy-2-deoxyguanosine (8-OHdG). An Absolute Human Telomere Lengths Quantification qPCR Assay kit and Telomerase Activity Quantification qPCR Assay kit (Nucleotestbio, Budapest, Hungary), as well as an 8-OHdG ELISA kit (Abbexa Ltd., Cambridge, United Kingdom) were used for analyses. Similar telomere lengths were found in GCs and FF, however telomerase activity was markedly depressed, while 8-OHdG levels were markedly elevated in FF compared with those in GCs (p < 0.01). Telomere lengths were independent of telomerase activity both in GCs and FF. However, GC 8-OHdG was inversely related to telomerase activity in GCs and FF (p < 0.05). Importantly, 8-OHdG levels both in GCs and FF had significant negative impact on the number of the retrieved and MII oocytes (p < 0.01), whereas FF 8-OHdG was negatively related further to the number of fertilized oocytes and blastocysts (p < 0.01). In conclusion, we could not confirm the direct association of telomere function and reproductive potential. However, oxidative DNA damage, as mainly reflected by 8-OHdG, adversely affected early markers of IVF outcome and clinical pregnancies.

The effects of ketogenic diet on beta-hydroxybutyrate, arachidonic acid, and oxidative stress in pediatric epilepsy

The exact mechanism of a ketogenic diet (KD) as a suitable alternative therapeutic approach for drug-resistant epilepsy (DRE) in alleviating seizures is not yet fully understood. The present study aimed to evaluate the role of the KD in reducing oxidative stress (OS) by increasing the ketone body beta-hydroxybutyrate (BHB) and Arachidonic acid (ARA), an essential polyunsaturated fatty acid, as a possible mechanism in relieving seizure attacks in children with DRE. Forty children with refractory epilepsy were included in the present study. The serum levels of BHB, ARA, and OS markers, malondialdehyde (MDA), and 8-hydroxyl-deoxyguanosine (8-OHdG), were evaluated in children with DRE and compared before and after the three months of KD therapy. Thirty-four of 40 included children could complete the three-month KD therapy. Twenty-one (61.76%) patients had more than a 50% reduction in seizure frequency after the KD (responders). The remaining 13 children were considered non-responders to the diet. The serum levels of ARA and BHB significantly (p < 0.05) increased after the KD therapy. The serum levels of OS parameters MDA and 8-OHdG before the diet therapy were significantly (p < 0.05) higher than those after the administration. The serum levels of BHB and MDA after the KD therapy in the responders were respectively higher and lower than those in the non-responders (p < 0.001). Ketogenic diet might reduce brain OS by increasing BHB and ARA. The role of BHB in diminishing OS and seizure might be more remarkable than ARA.

Effect of gambogenic acid in attenuating diethylnitrosamine (DEN)-induced hepatocellular carcinoma in rat model

Liver cancer, specifically hepatocellular carcinoma has been a widespread problem among general population. This study aims to investigate the modulating mechanism of gambogenic acid, a phenolic xanthonoid, in diethylnitrosamine (DEN)-induced liver cancer in rats. Male Wistar albino rats were clustered into four groups (n = 6). Group I served as control treated with normal saline. Hepatocellular carcinogenesis was induced in rats by single intraperitoneal (i.p.) administration of DEN in saline (200 mg/kg b.w.) for groups II and III. Group III received oral administration of gambogenic acid (20 mg/kg b.w.) one hour post DEN administration, whereas group IV received oral administration of gambogenic acid (20 mg/kg b.w.) alone. Rats were sacrificed after 16 weeks to determine the levels of hepatic biomarkers, oxidative stress markers, hematological profile and histopathological changes. Gambogenic acid significantly ameliorated the expressions of oxidative stress markers TBARS, GSH (P < 0.05), enzymatic antioxidants GPx, CAT, SOD, GST (P < 0.05), apoptosis mediators (P < 0.05), and serum biomarkers for liver damage and tumor formation (P < 0.05) compared with DEN-induced model group. Hepatocellular levels of 8-OHdG were significantly diminished (P < 0.05) by gambogenic acid against the damage incurred by DEN. Liver histopathological derangements caused by DEN were reversed by gambogenic acid. The results clearly impacted the effect of gambogenic acid in attenuating DEN-induced hepatocellular carcinoma in rats mediated through NF-kβ pathway and hepatocellular oxidative damage.

Evaluating the Levels of Oxidative DNA Damage, Antioxidant Profile and Pro-inflammatory Cytokines in Lung Cancer Patients

Eight-hydroxyguanosine (8-OHdG) is considered as one of the principle forms of oxygen radicals that stimulated the oxidative stress and has been extensively utilized as a biomarker for oncogenesis. The primary goal of the present study was to investigate the alteration in the levels of 8-OHdG, antioxidant profile and proinflammatory cytokines levels in patients with lung carcinoma. Blood samples were collected from 40 cases with lung cancer (stage III) admitted before the treatment, for health examination at the Nanakaly Hospital in Erbil city and 45 healthy samples of controls with ages ranging between 38-69 years for both groups. Circulating concentration of 8-OHdG, tumor necrosis factor and interleukin-6 were evaluated by ELISA. Circulating levels of superoxide dismutase (SOD), peroxidase (POX) and ascorbic acid (vitamin C) levels were also analyzed by using ELISA. The current work proposes that (8-OHdG) can be used as a functional biological marker, considering oxidative stress among the patients with lung carcinoma. The obtained data also indicated a correlation between serum cytokine concentrations and the rate of survival in lung carcinoma patients.

Association between urinary levels of 8-hydroxy-2-deoxyguanosine and F2a-isoprostane in male football players and healthy non-athlete controls with dietary inflammatory and antioxidant indices.

The relationship between the inflammatory and antioxidant potential of an athlete's diet and their oxidative biomarkers is an important area of investigation. Therefore, this study aimed to assess the excretion of 8-hydroxy-2-deoxyguanosine (8-OHdG) and F2alpha-isoprostane (F2a-IP) in the urine of male football players and healthy non-athlete controls. This study also aimed to examine the associations among the dietary inflammatory index (DII), the dietary total antioxidant capacity (DTAC), and the dietary phytochemical index (PI) with 8-OHdG and F2a-IP. In this descriptive-analytical study, 45 male football players and 45 healthy non-athletes, who were individually matched based on age and body mass index (BMI), were recruited from Shiraz City, Iran. Fasted urine samples were analyzed for 8-OHdG and F2a-IP levels. Anthropometric measurements were performed, and body composition was assessed using a body composition analyzer. A valid food frequency questionnaire (FFQ) was used to calculate DII, DTAC, and PI scores. Data analysis was conducted using a generalized estimating equation (GEE) model. We found that 8-OHdG (β = -6.96), F2a-IP (β = -82.58), and DII (β = -2.06) were significantly lower, while DTAC (β = 2.37) and PI (β = 0.084) were significantly higher in the football player group compared with the non-athlete group (P < 0.001 for all variables). In all participants, dietary indices were significantly associated with oxidative biomarkers. DII was positively associated with 8-OHdG (β = 2.25; P < 0.001) and F2a-IP (β = 38.34; P < 0.001). Furthermore, negative associations between DTAC (β = -1.42; P < 0.001) and PI (β = -35.37; P < 0.001) with 8-OHdG were found. Moreover, DTAC (β = -17.34; P < 0.001) and PI (β = -428.11; P = 0.003) were negatively associated with F2a-IP. The results of this study highlighted the importance of a healthy diet in reducing oxidative stress among football athletes. The levels of urinary biomarkers for DNA and lipid oxidation were found to be lower in football players compared to non-athletes. This suggests that following an anti-inflammatory and antioxidant-rich diet may help reduce oxidative stress in these individuals.

The Impact of Chitosan on Oxidative Stress in Liver of Rats Loaded With Lead Acetate

This study was conducted to determine the impact of chitosan on lead (Pb)-induced hepatotoxicity. Thus, lead acetate was administered intraperitoneally (50 mg/kg for 5 days) and chitosan was given as 200 mg/kg via oral gavage for 28 days. When the trial was terminated, it was determined that aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities in serum as well as Pb, 8-hydroxy-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels and catalase (CAT) activity in liver tissue significantly increased, however reduced glutathione (GSH) and ceruloplasmin (Cp) levels in liver and high density lipoproteins (HDL) in serum had a significant decrease in the Pb group when compared to the control group. The administration of chitosan significantly prevented Pb-induced changes in serum liver enzyme activities, 8-OHdG and MDA levels. In addition, chitosan showed a statistically insignificant effect on reduced GSH, Cp, HDL levels and CAT activity. Accordingly, administration of chitosan can strengthen the antioxidant defence system of liver tissue and may decrease oxidative stress.

1146 SLEEP DEPRIVATION INDUCES AGEING-LIKE CHANGES IN ANTIGRAVITY MUSCLES OF YOUNG ADULT MALE WISTAR RATS

Abstract Introduction Poor muscle health is associated with a series of chronic and metabolic conditions that are prevalent in individuals who chronically experience poor-quality sleep. But there is no study deciphering the role of sleep deprivation on muscle ageing. Therefore, in the present study we have measured the ultrastructure, histopathology, and oxidative stressors in soleus muscle of wistar rat after sleep deprivation and recovery sleep. Material and Methods The experiments were conducted in 18 rats of three groups. Group I rats had normal sleep wake cycle, Group II rats were subjected to 24 h sleep deprivation (SD) by gentle handling method1 and Group III rats had recovery sleep after 24 h SD. At the end of the sleep, sleep deprivation and recovery period, soleus muscle tissue was collected for ultrastructural, histological and oxidative stress markers. Oxidative damage was assessed by lipid peroxidation, catalase activity, reduced glutathione and nuclear labelling of 8-OHdG. The study was conducted as per the guidelines of the Institutional Animal Ethics Committee (960/IAEC/16). Results The data demonstrated that SD leads to ultrastructural changes in soleus muscle which includes sarcolemmal and mitochondrial alterations. In case of histopathological and histomorphological changes there was signs of tissue degeneration, inflammatory infiltrate in type I fibres and muscle atrophy was observed in soleus muscles. There was significant increase in level of 8-OHdG (p=0.02) and malondialdehyde in 24h SD (p=0.02) than control and recovery sleep groups. Moreover, the catalase activity and reduced glutathione level was significantly decreased in 24h SD group (p≤0.02) than control and recovery sleep. Conclusion 24hr sleep deprivation leads to an ageing like state in the skeletal muscle, which was recovered after sleep rebound.

Glyphosate exposure and urinary oxidative stress biomarkers in the Agricultural Health Study.

Glyphosate is the most widely applied herbicide worldwide, and its use has been associated with increased risks of certain hematopoietic cancers in epidemiologic studies. Animal and in vitro experiments suggest that glyphosate may induce oxidative stress, a key characteristic of carcinogens; however, evidence in human populations remains scarce. We investigated associations between glyphosate exposure and urinary oxidative stress biomarkers in the Biomarkers of Exposure and Effect in Agriculture study, a molecular epidemiologic subcohort in the Agricultural Health Study. This analysis included 268 male farmers selected based on self-reported recent and lifetime occupational glyphosate use and 100 age- and geography-matched male nonfarmers. Concentrations of glyphosate and oxidative stress biomarkers (8-hydroxy-2'-deoxyguanosine [8-OHdG], 8-iso-prostaglandin-F2α, and malondialdehyde [MDA]) were quantified in first-morning-void urine. We performed multivariable linear regression to evaluate associations of urinary glyphosate and self-reported glyphosate use with each oxidative stress biomarker. Urinary glyphosate concentrations were positively associated with levels of 8-OHdG (highest vs lowest glyphosate quartile; geometric mean ratio = 1.15, 95% confidence interval = 1.03 to 1.28; Ptrend = .02) and MDA (geometric mean ratio = 1.20, 95% confidence interval = 1.03 to 1.40; Ptrend = .06) overall. Among farmers reporting recent glyphosate use (last 7 days), use in the previous day was also associated with statistically significantly increased 8-OHdG and MDA levels. Compared with nonfarmers, we observed elevated 8-iso-prostaglandin-F2α levels among farmers with recent, high past 12-month, or high lifetime glyphosate use. Our findings contribute to the weight of evidence supporting an association between glyphosate exposure and oxidative stress in humans and may inform evaluations of the carcinogenic potential of this herbicide.

Oxidative DNA Damage in Relation to the Severity of COVID-19 Infection in Duhok City, Kurdistan Region- Iraq

Coronavirus disease 2019 (COVID-19) has rapidly spread across the globe since its outbreak in Wuhan, China, in 2019. Clinical evidence suggests higher oxidative stress in COVID-19 patients, and this worsening redox status which may contribute to disease progression. The present study aimed to investigate the oxidative Deoxyribonucleic acid damage in patients with mild and severe COVID-19 infection and to evaluate its relationship to the disease progression and severity. A case-control study was conducted from September 2021 to January 2022 in Duhok city, Kurdistan Region-Iraq. 180 individuals have participated. Among 88 COVID-19 cases, 92 healthy volunteers as the control group, with ages ranging (18-45) years. Patients were divided into two groups according to the severity of infection (mild cases, severe cases). Serum level of 8-OHdG and malondialdehyde (MDA) were assessed as oxidative stress biomarkers. Serum levels of 8-OHdG were considerably higher in patients with COVID-19 infection in comparison to the control group, (p&lt;0.01). The further statistical analysis has revealed a significantly higher 8-OHdG in blood in female cases with severe COVID-19 infection compared cases with a mild infection, (p&lt;0.01). Serum MDA levels in severe cases were higher, statistically significant when compared with the control group (p=0.007). Severe cases had higher level of MDA than in mild case, in male cases (p&lt;0.05) in female cases (p&lt;0.0001). The current data suggest that patients who were infected severely with COVID-19 are under huge oxidative stress attack. Analysis of data shows that severe cases of COVID-19 infection had significantly greater level of serum 8-OHdG than in healthy control subjects.

Neutralization of iron oxide magnetic nanoparticle aquatoxicity on Oncorhynchus mykiss via supplementation with ulexite

Nowadays, the unique features of nanoparticles (NPs) have encouraged new applications in different areas including biology, medicine, agriculture, and electronics. Their quick joining into daily life not only enhances the uses of NPs in a wide range of modern technologies but also their release into the aquatic environment causes inevitable environmental concerns. On the other hand boron exhibits key physiological effects on biological systems. This research was designed for evaluating the toxicity of magnetite nanoparticles (Fe3O4-MNPs) on aquatic organisms and obtaining data for the information gap in this area. In this study, Rainbow trout (Oncorhynchus mykiss) was considered as an aquatic indicator, and trials were designed as Ulexite (a boron mineral, UX) treatment against exposure to Fe3O4-MNPs. Synthesized and characterized Fe3O4-MNPs were exposed to rainbow trouts in wide spectrum concentrations (0.005–0.08 mL/L) to analyze its lethal dose (LC50) and cytoprotective properties by UX treatment were assessed against Fe3O4-MNPs applications for 96 h. For the initial toxicity analysis, hematological parameters (blood cell counts) were examined in experimental groups and micronucleus (MN) assay was performed to monitor nuclear abnormalities after exposure to NPs. Biochemical analyzes in both blood and liver samples were utilized to assess antioxidant/oxidative stress and inflammatory parameters. Also, 8-hydroxy-2'-deoxyguanosine (8-OHdG) assay was used to investigate oxidative DNA lesions and Caspase-3 analysis was performed on both blood and liver tissues to monitor apoptotic cell death occurrence. When antioxidant enzymes in blood and liver tissue were examined, time-dependent decreases in activity were determined in SOD, CAT, GPx, and GSH enzymes, while increased levels of MDA and MPO parameters were observed in respect to Fe3O4-MNPs exposure. It was found that TNF-α, Il-6 levels were enhanced against Fe3O4-MNPs treatment, but Nrf-2 levels were decreased at the 46th and 96th h. In the 96th application results, all parameters were statistically significant (p < 0.05) in blood and liver tissue, except for the IL-6 results. It was determined that the frequency of MN, the level of 8-OHdG and caspase-3 activity increased in respect to Fe3O4-MNPs exposure over time. Treatment with UX alleviated Fe3O4-MNPs-induced hematotoxic and hepatotoxic alterations as well as oxidative and genetic damages. Our findings offer strong evidence for the use of UX as promising, safe and natural protective agents against environmental toxicity of magnetite nanoparticles.

Heat Exposure and Oxidative DNA Damage Among Bakery Workers in Duhok Province, Iraq

Background and rationales: There are not much researches on the effects of heat exposure, specifically on oxidative DNA damage, among bakery employees. Physical labor-intensive baking exposes bakers to high temperatures, especially in traditional bakeries with uncovered ovens. An electronic heat measuring device index and serum 8-hydroxy-2-deoxy guanosine(8-OHdG) will be utilized in this study to quantify oxidative DNA damage and heat stress, respectively. The impact of heat exposure on oxidative DNA damage in the Duhok populace has not yet been studied in the literature. This study aimed to measure the impact of heat exposure on serum levels of oxidative DNA damage in bakers who work in high-temperature bakeries. Method: A case control study was carried out among 141 participants, 62 individuals exposed to heat (bakery workers) and 79 individuals unexposed to heat exposure as control group. 8-hydroxy 2-deoxy guanosine (8-OHdG) has been analyzed using Enzyme-Linked Immunosorbent Assay (ELISA). Results: The mean ±SD of 8-OHdG in bakery workers (13.78 ±5.89 ng/ml) were higher than those in healthy control individuals (1.55 ±0.75 ng/ml) with a statistically significantly differences (p&lt;0.0001). Conclusion: High mean levels of 8-OHdG was found in exposed individuals (bakery workers) in comparison with control individuals unexposed to heat, suggesting that high-heat exposures have the risk of causing genetic effects and DNA damage.

Effects of lipids on inflammation and DNA damage in diabetes mellitus patients

We aimed to see the effect of lipids on 8-hydroxy-2'-deoxyguanosine (8-OHdG), a marker of DNA damage, and Interleukin-6 (IL-6), an inflammatory cytokine, in Diabetes Mellitus (DM) patients. Serum IL-6, and 8-OHdG levels were determined using kits based on the ELISA principle. The groups were different in terms of total cholesterol, LDL cholesterol and triglyceride. Group 2 (dyslipidemia positive DM positive) was significantly higher than the other groups. There was no statistical difference between group 1 (dyslipidemia negative DM positive) and the control group (p&lt;0.001). 8-OHdG levels differ between groups. The level of 8-OHdG was significantly higher in group 2 compared to the other groups (p&lt;0.001). IL-6 was different between groups. Group 2, IL-6 level was found to be significantly higher than the other groups (p&lt;0.001). A positive correlation was found between serum 8-OHdG level and total cholesterol (r=0.417 p&lt;0.001), LDL cholesterol (r=0.491 p&lt;0.001) and triglyceride (r=0.441 p&lt;0.001). A positive correlation was found between IL-6 and total cholesterol (r=0.522 p&lt;0.001), LDL cholesterol (r=0.646 p&lt;0.001) and triglyceride (r=0.495 p&lt;0.001). IL-6 and 8-OHdG levels are high in dyslipidemic DM patients with a high lipid profile. IL-6 and 8-OHdG levels are elevated in dyslipidemic DM patients with a high lipid profile. IL-6 and 8-OHdG can be used as biomarkers to evaluate dyslipidemic complications seen in DM patients in the early diagnosis.

Study on the Mechanism of Improving Mice with Atherosclerosis Using Dendrocrepine

Aim: To discuss Den on apoptosis and Nrf2/ARE in atherosclerotic vulnerable plaque of apolipoprotein in E (ApoE)-/- mice. Methods: Randomly dividing ApoE-/- mice as 5 groups including Normal, Model, Den-L (10 mg/kg), Den-M (20 mg/kg) and Den-H (40 mg/kg) groups. The atherosclerotic vulnerable plaque model was established by high-fat feeding and right common carotid artery catheterization (perivascular carotid collar placement, PCR), and Den was given by difference concentration Den, the pathological changes of right common carotid arery, apoptosis of vascular smooth muscle, Bax, Bcl-2 and Caspase-3 proteins expression using IHC and WB assay, Nrf2, ARE and MDA, 8-OHdG and TAC levels were detected. Results: Model group showed typical pathological changes of vulnerable plaque, the apoptosis cell number, Bax, Caspase-3 and MDA, 8-OHdG significantly increased, the Bcl-2, Nrf2, ARE and TAC levels significantly decreased (P &lt; 0.001, respectively); Compared with model group, the plaque of Den groups were reduced and tended to be stable, the apoptosis cell number, Bax, Caspase3 and MDA, 8-OHdG levels significantly decreased, Bcl-2, Nrf2, ARE and TAC levels significantly increased (P&lt;0.05, respectively). Conclusion: Den improves atherosclerotic vulnerable plaque of ApoE-/- mice, which is related to the inhibition of apoptosis and Nrf2/ARE pathway.

Protective Effects of Fuziline on Dobutamine-Induced Heart Damage in Mice.

Fuziline is one of the many antioxidants currently being tested to treat cardiac damage. In our study, histopathological and biochemical effects of fuziline were investigated in mice with dobutamine-induced heart damage in vitro. Thirty-two adult male BALB/c mice, average weight of 18-20 g, were randomly divided into four groups - Group 1 (sham, n=8), Group 2 (control, dobutamine, n=8), Group 3 (treatment 1, dobutamine + fuziline, n=8), and Group 4 (treatment 2, fuziline, n=8). Biochemical parameters and total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) values were measured. Interleukin 1 beta (IL-1β), NLR family, pyrin domain containing protein 3 (NLRP3), 8-hydroxy-deoxyguanosine (8-OHDG), gasdermin D (GSDMD), and galectin 3 (GAL-3) levels were analyzed by enzyme-linked immunosorbent assay method, and histopathological examination of heart tissues was performed. When dobutamine + fuziline and fuziline groups were compared, troponin-I (P<0.05), NLRP3 (P<0.001), GSDMD (P<0.001), 8-OHDG (P<0.001), IL-1β (P<0.001), and GAL-3 (P<0.05) were found to be statistically significant. TOS level was the highest in the dobutamine group (P<0.001) and TAS level was the highest in the fuziline group (P<0.001). OSI level was statistically significant between the groups (P<0.001). In histopathological examination, focal necrosis areas were smaller in the dobutamine + fuziline group than in the dobutamine group, and cardiac myocytes were better preserved. Fuziline markedly reduced cardiac damage and pyroptosis in mice with dobutamine-induced heart damage by lowering the levels of GSDMD, 8-OHDG, IL-1β, and GAL-3. It also prevented necrosis of cardiac myocytes in histopathological evaluation.

Immune system modulation by low-dose ionizing radiation-induced adaptive response.

Hormesis or low-dose ionizing radiation is known to induce various biological responses, a subcategory of which is the adaptive response, which has been reported to protect against higher radiation doses via multiple mechanisms. This study investigated the role of the cell-mediated immunological component of low-dose ionizing radiation-induced adaptive response. Herein, male albino rats were exposed to whole-body gamma radiation, using a Cs137 source with low-dose ionizing radiation doses of 0.25 and 0.5Gray (Gy); 14 days later, another irradiation session at a dose level of 5Gy was carried on. Four days post-irradiation at 5Gy, rats were sacrificed. The low-dose ionizing radiation-induced immuno-radiological response has been assessed through the T-cell receptor (TCR) gene expression quantification. Also, the serum levels of each of interleukins-2 and -10 (IL-2, IL-10), transforming growth factor-beta (TGF-β), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) were quantified. Results indicated that priming low irradiation doses resulted in significant decrements in TCR gene expression and the serum levels of IL-2, TGF-β, and 8-OHdG with an increment in IL-10 expression compared to the irradiated group, which did not receive low priming doses. The observed low-dose ionizing radiation-induced radio-adaptive response significantly protected against high irradiation dose injuries, through immune suppression, representing a promising pre-clinical protocol that would be applied to minimize radiotherapy side effects on normal but not against the tumor cells.

Severe COVID-19 classified by SCRI is associated with higher levels of AOPP and 8- OHDG.

SARS-CoV-2 has become one of the most important and challenging medical research topics in recent years. The presence of endothelial dysfunction, immune thrombosis, and oxidative stress contributes to complications and requires more extended hospitalisation of patients. In this article, we focused on analysing the impact of oxidative stress on the severity of COVID-19 infection. The study group consisted of 72 patients with laboratory-confirmed SARS-CoV enrolled. The patients were divided into moderate and severe diseases according to the SCRI (Simple Covid Risk Index, including lymphocyte/D-dimer ratio). Using the ELISA kit, we determined the level of AOPP and 8-OHdG. Patients with severe COVID-19 had higher levels of both AOPP (P<0.05) and 8-OHdG (P<0.05) compared to patients with moderate disease. Albumin levels were significantly lower (P<0.001), although fibrinogen (P<0.01), D-dimer (P<0.001), and TF (P<0.05) levels were higher in severe patients than in moderate course. AOPP/Alb was also higher among severe patients (P<0.05). Our data suggest a potential role for AOPP and 8-OHdG in predicting the outcome of SARS-CoV-2 patients. Elevated AOPP levels were associated with increased Dimer-D, TF, and vWF activity levels.