Study on the Mechanism of Improving Mice with Atherosclerosis Using Dendrocrepine

Abstract

Aim: To discuss Den on apoptosis and Nrf2/ARE in atherosclerotic vulnerable plaque of apolipoprotein in E (ApoE)-/- mice. Methods: Randomly dividing ApoE-/- mice as 5 groups including Normal, Model, Den-L (10 mg/kg), Den-M (20 mg/kg) and Den-H (40 mg/kg) groups. The atherosclerotic vulnerable plaque model was established by high-fat feeding and right common carotid artery catheterization (perivascular carotid collar placement, PCR), and Den was given by difference concentration Den, the pathological changes of right common carotid arery, apoptosis of vascular smooth muscle, Bax, Bcl-2 and Caspase-3 proteins expression using IHC and WB assay, Nrf2, ARE and MDA, 8-OHdG and TAC levels were detected. Results: Model group showed typical pathological changes of vulnerable plaque, the apoptosis cell number, Bax, Caspase-3 and MDA, 8-OHdG significantly increased, the Bcl-2, Nrf2, ARE and TAC levels significantly decreased (P < 0.001, respectively); Compared with model group, the plaque of Den groups were reduced and tended to be stable, the apoptosis cell number, Bax, Caspase3 and MDA, 8-OHdG levels significantly decreased, Bcl-2, Nrf2, ARE and TAC levels significantly increased (P<0.05, respectively). Conclusion: Den improves atherosclerotic vulnerable plaque of ApoE-/- mice, which is related to the inhibition of apoptosis and Nrf2/ARE pathway.