7th Generation Research Articles

Differential expression of the miR-17-92 cluster and miR-17 family in breast cancer according to tumor type; results from the Norwegian Women and Cancer (NOWAC) study

BackgroundMicroRNAs (miRNAs) are promising biomarkers due to their structural stability and distinct expression profile in various cancers. We wanted to explore the miRNA expression in benign breast tissue and breast cancer subgroups in the Norwegian Women and Cancer study.MethodsSpecimens and histopathological data from study participants in Northern Norway diagnosed with breast cancer, and benign tissue from breast reduction surgery were collected. Main molecular subtypes were based on surrogate markers; luminal A (ER+ and/or PR+, HER2− and Ki67 ≤ 30%), luminal B (ER+ and/or PR+, HER2− and Ki67 > 30% or ER+ and/or PR+ and HER2+), HER2 positive (ER− and PR− and HER2+) and triple-negative (ER−, PR− and HER2−). RNA was extracted from formalin-fixed paraffin-embedded (FFPE) tissue, and miRNAs were successfully analyzed in 102 cancers and 36 benign controls using the 7th generation miRCURY LNA microarray containing probes targeting all human miRNAs as annotated in miRBASE version 19.0. Validation with RT-qPCR was performed.ResultsOn average, 450 miRNAs were detected in each sample, and 304 miRNAs were significantly different between malignant and benign tissue. Subgroup analyses of cancer cases revealed 23 miRNAs significantly different between ER+ and ER− tumors, and 47 miRNAs different between tumors stratified according to grade. Significantly higher levels were found in high grade tumors for miR-17-5p (p = 0.006), miR-20a-5p (p = 0.007), miR-106b-5p (p = 0.007), miR-93-5p (p = 0.007) and miR-25-3p (p = 0.015) from the paralogous clusters miR-17-92 and miR-106b-25. Expression of miR-17-5p (p = 0.0029), miR-20a-5p (p = 0.0021), miR-92a-3p (p = 0.011) and miR-106b-5p (p = 0.021) was significantly higher in triple-negative tumors compared to the rest, and miR-17-5p and miR-20a-5p were significantly lower in luminal A tumors.ConclusionsmiRNA expression profiles were significantly different between malignant and benign tissue and between cancer subgroups according to ER− status, grade and molecular subtype. miRNAs in the miR-17-92 cluster and miR-17 family were overexpressed in high grade and triple-negative tumors associated with aggressive behavior. The expression and functional role of these miRNAs should be further studied in breast cancer to explore their potential as biomarkers in diagnostic pathology and clinical oncology.

Role of ovarian tumor stem-like cells sorted from human epithelial ovarian cancer SKOV3 cells in vasculogenic mimicry formation

To isolate tumor stem-like cells from human epithelial ovarian cancer SKOV3 cells and explore their role in the formation of vascularization mimicry (VM). SKOV3 cells were passaged to the 7th generation by suspension culture in serum-free medium, and the percentages of CD133- and CD117-positive cells in the 1st, 3rd, 5th and 7th generations were analyzed using flow cytometry. The proliferative activity of the cells sorted from the 7th generation SKOV3 cells was assessed with colony formation assay. A three-dimensional cell culture model was established to compare the ability of VM formation between the sorted cells and the parental SKOV3 cells. The expression levels of matrix metalloproteinases-2 (MMP-2) and MMP-9 in the two groups were detected using real-time PCR and Western blotting. Some SKOV3 cells formed typical cell spheres with suspension growth in serum-free medium and were passaged to the 7th generation. Flow cytometry revealed that the percentage of CD133-positive cells increased with cell passaging. The cloning efficiency of the sorted cells was significantly higher than that of the parental SKOV3 cells (50.33% vs 5.33%, P < 0.001). The VM formation ability of the sorted cells was stronger than that of the parental SKOV3 cells in the three-dimensional cell culture system. RT-PCR and Western blotting showed that the expression levels of MMP-2 and MMP-9 were significantly higher in the 7th passage cells than in the parental cells (P < 0.05). The sorted cells from SKOV3 cells cultured in serum-free medium exhibit biological properties of tumor stem cells with strong VM formation ability, suggesting their role in VM formation.

Paradox of Middleware: Innovation Enabler and Creativity Constraint

A critical determinant of the success of digital platforms is the availability of third-party complements. In order to foster the creation of such complements, platforms often rely on boundary resources: software tools and libraries that support and lower the costs of developing complementary innovations. However, while such boundary components are increasingly widespread, little is understood about how these components shape subsequent innovation outcomes and the overall platform generativity. Here, we focus on a particularly important form of boundary resource for digital platforms: Middleware components for software development. We theorize how middleware may shape both the novelty of products being created and their subsequent market value, and propose a number of mechanisms for these effects, for which we provide evidence. We test for these different mechanisms by looking at the development of console games during 6th and 7th generation of gaming consoles. We find that the introduction of licensed middleware such as third-party game and graphics engines lead to the creation of less novel, but more commercially successful products. We attribute this to the fact that middleware allows firms to reallocate resources from developing basic functionality (such as programming how the graphics will be rendered for the target platform), to other elements that make games more valuable and successful on the market (such as story or game mechanic innovation). Our results have implications for how we think about the impact of boundary resources such as middleware on digital platforms, and recombinant innovation more broadly.

ASSESSMENT OF SHEAR BOND STRENGTH OF RESTORATIVE MATERIALS IN VARIOUS DEPTH OF HARD DENTAL TISSUE PREPARATION IN FRONT TEETH

In modern dentistry, there are two different approaches to the restoration of anatomy and aesthetics of anterior teeth. The most common is the option of direct restoration with using composite resin materials. The second option is the restoration with using ceramic materials that is carried out through the cooperation of the dentists and the dental laboratory technicians, or, alternatively, with various CAD-CAM systems directly at the dental office. This experiment was carried out by using teeth (premolars, incisors) without significant loss of hard dental tissues. Teeth with abrasion (all except 1st degree) were excluded from the test group. Within the framework of the planned study, the selected extracted teeth were prepared (a certain volume of hard tissues was removed from the vestibular side (a subgroup of the teeth prepared within in the enamel layer, and a subgroup of teeth with the exposed dentine). A total of 40 teeth were prepared (20 teeth with the exposed dentine, 20 with the enamel preparation). The tests were carried out by using a universal testing machine AUTOGRAPH AGS-J. A sample with a tooth was placed at the top of the device then the second part of the sample containing the plate of the material (composite or ceramic), was covered with the second part of the device to be tested, and neatly, trying to avoid applying tension to the adhesive butt joints, and then fastened to the lower clamping device of the tension testing machine. We created the shear forces before the sample was destroyed, paying attention to the character of adhesion line oddments. The results of the experiment have demonstrated that the most optimal shear strength is found out in the complex of hard tooth tissues and ceramic materials IPS Empress Aesthetic and EX3, in the teeth prepared within the tooth enamel layer. When using composite resin, the values studied are lower by 11,4% and 18,1%, (application of adhesion systems of 5th and 7th generations, respectively). In the cases when dentin is exposed, the shear strength decreases by 19,5% when using ceramics modified by leucite glass, by 17,3% in the case of application of feldspar ceramics, and by 8,4% and 13,2% for composite resin restoration when using adhesive systems of 5th and 7Ith generations respectively.

INVESTIGATIIONT OF CONIC FLOW POINT IN ADHESIVE SYSTEMS BY USING HEPPLER CONSISTOMETER

When restoring wedge-shaped tooth defects, the adhesive system, and in particular, its microhardness provided by an inorganic filler plays a significant role in withstanding the masticatory load. The conic flow point is a measure for the microhardness of spatially-stitched polymer matrices and depends on the nature, structure, density of the polymer chains. The purpose of this work is to compare the properties of analogues: the Conic Flow Point of 5th DC Adhesive NF Adhesive Systems (Dental Central) and the 7th Single Bond (3M ESPE) by using Heppler consistometer under laboratory conditions.
 The study was carried out on adhesive plates manufactured directly in the laboratory before testing. The samples were divided into two groups depending on the adhesive system used: 10 samples of 5 generations (DC Adhesive NF, Dental Center) and 10 samples of 7 generations (Single bond Universal 3M ESPE). To obtain specimens of adhesive systems of 3.0 ± 0.2 mm high, a metallic mould covered with silicone was used. On the back side of the mould we put a sheet of lavsan film, turned the mould upside-down, then put the adhesive into the mould and covered with lavsan film. Then this sample was irradiated with a photopolymerizer for 40 seconds. Without removing the film, the sample was turned over and its other side was irradiated for 40 seconds. Then we peel off the film and take out finished sample from the mould. Prior to testing, samples were kept in distilled water at a temperature of (37 ± 2) ºC for 24 hours.
 According to relevant literature data and research data reported by 3M ESPE and Dental Central firms, no studies for assessing conic flow point by using Heppler consistometer for 5th and 7th generation adhesives were carried put under laboratory conditions. Out test results have demonstrated the regularity that the harder an adhesive is, the higher values of the conical flow point. That is, the adhesive can withstand a load that is positive for retorting cervical defects.
 Thus, the restoration of wedge-shaped defects requires a competent and differentiated approach to the choice of adhesive system in order to obtain positive results and to avert spreading of pathological process that ensured the treatment efficacy. The use of 5th generation AD Adhesive NF promotes the formation of a complete hybrid layer, firm bonding between composite material, dentin and enamel, provides effective sealing at the edge of filling margins with dental tissues and their resistance due to adhesive ductility: all this guarantees the durability of the restorations performed.

Prediction of annual generations of the tomato leaf miner Tuta absoluta on tomato cultivations in Egypt

BackgroundTomato crop is the first vegetable crop in Egypt; it covers about 3% of Egypt’s total planted area. The tomato leaf miner Tuta absoluta is a polyphagous insect pest which feeds on several solanaceous plant species and preferentially on tomato causing high losses in productivity. This study aims to predict the annual generations and expected times for moth emergence in order to select the best time for the pest management.MethodsSpecimens of T. absoluta were collected from infested tomato field and reared under controlled temperatures to calculate the thermal requirements for development and completing the whole generation. The duration of different developmental stages of this insect and the life table parameters were estimated. The data were used to calculate the thermal constant and developmental zero for different developmental stages using the formula of Jasic (Bihemoslov 72:383-390, 1975) mathit{mathsf{y}}=mathit{mathsf{n}} left(mathit{mathsf{t}}-mathit{mathsf{x}}right) .ResultsEcological studies were performed to evaluate the adverse effects of climatic changes on insect populations based on accumulation of the thermal requirements for development. The developmental zero was 5.79 °C and the thermal constant was 171 DDs to complete the pupal stage development. Based on these values, the pupae development can be repeated 36 times during 2012 when the mean annual temperature was 22.8 °C. In Giza governorate, the average life cycle duration during 2012 was 52.44 days when the mean annual temperature was 22.8 °C, the number of annual generations was 8.05, and the timing of the first generation was 11.66 days in April 2012 while the 8th generation cycle was obtained after 28.2 days of January 2013. In Qena governorate, the mean annual temperature was increased to be 26.04 °C, so the annual generations increased to 9.52. The first generation was recorded by the end of April 2012 while the 9th generation was completed on 24.4 days of December 2012. In Merssa-Matrouh governorate, the mean annual temperature was 20.53 °C and the number of the annual generations was found to be 7 during 2012. The first generation occurred on the day 19.86 of April 2012 while the 7th generation cycle was detected after 30.98 days of January 2013. This means that there must be successive emergence of the adults but with variations during the different months and locations in correlation with variation of temperatures.ConclusionThe accumulated thermal requirements appeared to be a very critical factor affecting insect development to complete the annual generations of T. absoluta in Egypt. This helps in designing the effective program and the best time for the pest management.

Identification of selection signatures involved in performance traits in a paternal broiler line

BackgroundNatural and artificial selection leads to changes in certain regions of the genome resulting in selection signatures that can reveal genes associated with the selected traits. Selection signatures may be identified using different methodologies, of which some are based on detecting contiguous sequences of homozygous identical-by-descent haplotypes, called runs of homozygosity (ROH), or estimating fixation index (FST) of genomic windows that indicates genetic differentiation. This study aimed to identify selection signatures in a paternal broiler TT line at generations 7th and 16th of selection and to investigate the genes annotated in these regions as well as the biological pathways involved. For such purpose, ROH and FST-based analysis were performed using whole genome sequence of twenty-eight chickens from two different generations.ResultsROH analysis identified homozygous regions of short and moderate size. Analysis of ROH patterns revealed regions commonly shared among animals and changes in ROH abundance and size between the two generations. Results also suggest that whole genome sequencing (WGS) outperforms SNPchip data avoiding overestimation of ROH size and underestimation of ROH number; however, sequencing costs can limited the number of animals analyzed. FST-based analysis revealed genetic differentiation in several genomic windows. Annotation of the consensus regions of ROH and FST windows revealed new and previously identified genes associated with traits of economic interest, such as APOB, IGF1, IGFBP2, POMC, PPARG, and ZNF423. Over-representation analysis of the genes resulted in biological terms of skeletal muscle, matrilin proteins, adipose tissue, hyperglycemia, diabetes, Salmonella infections and tyrosine.ConclusionsIdentification of ROH and FST-based analyses revealed selection signatures in TT line and genes that have important role in traits of economic interest. Changes in the genome of the chickens were observed between the 7th and 16th generations showing that ancient and recent selection in TT line may have acted over genomic regions affecting diseases and performance traits.

All - Silicon Carbide Power Modules Based High Performance Inverter for Traction Applications

The Silicon (Si) IGBT was an enormous positive disruption to the area of Power Electronics in the 1980s. It has been the workhorse of the industry ever since. It is currently available in its 7th generation. The next revolutionary technology is power semiconductor devices made with wide bandgap materials like Silicon Carbide (SiC). SiC devices have higher critical breakdown strength, wider bandgap, thinner drift layer and better heat conductivity when compared to Sidevices. A traction inverter using SiC devices will have low value of conductivity loss, lower values of switching and recovery losses, increased range in terms of switching frequency and temperature with better heat dissipation. The converter will be smaller and lighter due to less cooling requirements. A study was done on various options available. Efficiency was compared using an improved model of SiC MOSFET.

PERKEMBANGAN KESENIAN JARAN JENGGO ASWO KALOKO JOYO GENERASI KE-6 SAMPAI GENERASI KE-7 DESA SOLOKURO KECAMATAN SOLOKURO KABUPATEN LAMONGAN

Jaran Jenggo art is a circumcision dress art procession using a horse or jaran accompanied by jedor music. Jaran Jenggo art began to develop from the 6th generation to the 7th generation which has been implemented because of various aspects of modern thought, education and economics Making Jaran Jenggo Art Aswo Kaloko Joyo makes art form innovation so that it is not monotonous. It is this change that motivates. Jaran Jenggo Aswo Kaloko Joyo developed the form. The aim of the study was to find out the development. Jaran Jenggo Aswo Art Kaloko Joyo from the 6th generation to the 7th day. This study uses qualitative methods through the Sociology of Arts approach to encode the development of society towards artists in the creation of films. Jaran Jenggo to make it look more attractive. Social change, making Jaran Jenggo Art finally began to rise with innovative forms, accompaniment, costumes and make-up, even to the stage that consisted of the farewell stage which began in the 6th Generation to become a new form and developed until the 7th generation ofization. Jaran Jenggo Art Aswo Kaloko Joyo has found a country that is mature enough in the mood, with the development that creates Jaran Jenggo still has to improve the quality and quality of the art they have.

Paradox of Middleware: Innovation Enabler and Creativity Constraint

A critical determinant of the success of digital platforms is the availability of third-party complements. In order to foster the creation of such complements, platforms often rely on boundary resources: software tools and libraries that support and lower the costs of developing complementary innovations. However, while such boundary components are increasingly widespread, little is understood about how these components shape subsequent innovation outcomes and the overall platform generativity. Here, we focus on a particularly important form of boundary resource for digital platforms: Middleware components for software development. We theorize how middleware may shape both the novelty of products being created and their subsequent market value, and propose a number of mechanisms for these effects, for which we provide evidence. We test for these different mechanisms by looking at the development of console games during 6th and 7th generation of gaming consoles. We find that the introduction of licensed middleware such as third-party game and graphics engines lead to the creation of less novel, but more commercially successful products. We attribute this to the fact that middleware allows firms to reallocate resources from developing basic functionality (such as programming how the graphics will be rendered for the target platform), to other elements that make games more valuable and successful on the market (such as story or game mechanic innovation). Our results have implications for how we think about the impact of boundary resources such as middleware on digital platforms, and recombinant innovation more broadly.

Quantitative CT detects changes in airway dimensions and air-trapping after bronchial thermoplasty for severe asthma

ObjectivesBronchial thermoplasty (BT) can be considered in the treatment of severe asthma to reduce airway smooth muscle mass and bronchoconstriction. We hypothesized that BT may thus have long-term effects on airway dimensions and air-trapping detectable by quantitative computed tomography (QCT). MethodsPaired in- and expiratory CT and inspiratory CT were acquired in 17 patients with severe asthma before and up to two years after bronchial thermoplasty and in 11 additional conservatively treated patients with serve asthma, respectively. A fully automatic software calculated the airways metrics for wall thickness (WT), wall percentage (WP), lumen area (LA) and total diameter (TD). Furthermore, lung air-trapping was quantified by determining the quotient of mean lung attenuation in expiration vs. inspiration (E/I MLA) and relative volume change in the Hounsfield interval −950 to −856 in expiration to inspiration (RVC856-950) in a generation- and lobe-based approach, respectively. ResultsBT reduced WT for the combined analysis of the 2nd–7th airway generation significantly by 0.06 mm (p = 0.026) and WP by 2.05% (p < 0.001), whereas LA and TD did not change significantly (p = 0.147, p = 0.706). No significant changes were found in the control group. Furthermore, E/I MLA and RVC856-950 decreased significantly after BT by 12.65% and 1.77% (p < 0.001), respectively. ConclusionBT significantly reduced airway narrowing and air-trapping in patients with severe asthma. This can be interpreted as direct therapeutic effects caused by a reduction in airway-smooth muscle mass and changes in innervation. A reduction in air-trapping indicates an influence on more peripheral airways not directly treated by the BT procedure.

Comparison the bond strength of metal orthodontic brackets bases using three types of adhesive generations

Sixty stainless steel brackets were divided into two groups, group I used coarsemesh base type (Dentaurum); group II used casted mesh base type (Orthoorganizer).Each group was subdivided into three sub groups (Ten brackets for each), all thebrackets were bonded to sixty sound freshly extracted human upper first premolarsteeth using Microfilled composite material cured with light emitted diode (LED) lightcure unit. The first sub group was bonded with 5th generation bonding system; thesecond group was bonded with 6th generation bonding system, while the third subgroup bonded with 7th generation bonding system.The results of this study revealed that the 7th generation shows higher shear bondstrength than other two groups. The brackets with casted mesh type base demonstratehigher shear bond strength than brackets with coarse mesh type base.

Effect of miR-21 down-regulated by H 2O 2 on osteogenic differentiation of MC3T3-E1 cells

To explore the effect and mechanism of miR-21 down-regulated which was induced by H 2O 2 on osteogenic differentiation of MC3T3-E1 cells. MC3T3-E1 cells were cultured and passaged, and the 7th generation cells were harvested to use in experiment. The MC3T3-E1 cells were treated with different concentrations (0, 40, 80, 160, and 320 μmol/L) of H 2O 2. The expression of miR-21 was detected by real-time quantitative PCR (RT-PCR) and the cell viability was determined by MTS. Then the appropriate concentration of H 2O 2 was obtained. To analyze the effect of H 2O 2 on osteogenic differentiation of MC3T3-E1 cells, the MC3T3-E1 cells were divided into blank control group (group A), H 2O 2 group (group B), osteogenic induction group (group C), and H 2O 2+osteogenic induction group (group D). The expression of miR-21 and the osteogenesis related genes expressions of Runx2, osteopontin (OPN), and collagen type Ⅰ alpha 1 (Col1a1) were detected by RT-PCR. The expression of phosphatase and tensin homolog (PTEN) was detected by Western blot. The extracellular calcium deposition was detected by alizarin red staining. To analyze the effect on osteogenic differentiation of MC3T3-E1 cells after the transfection of miR-21 inhibitor and siRNA-PTEN, the MC3T3-E1 cells were divided into H 2O 2 group (group A1), H 2O 2+osteogenic induction group (group B1), H 2O 2+osteogenic induction+miR-21 inhibitor group (group C1), and H 2O 2+osteogenic induction+miR-21 inhibitor negative control group (group D1); and H 2O 2 group (group A2), H 2O 2+osteogenic induction group (group B2), H 2O 2+osteogenic induction+siRNA-PTEN negative control group (group C2), and H 2O 2+osteogenic induction+siRNA-PTEN group (group D2). The osteogenesis related genes were detected by RT-PCR and the extracellular calcium deposition was detected by alizarin red staining. The results of MTS and RT-PCR showed that the appropriate concentration of H 2O 2 was 160 μmol/L. The expression of miR-21 was significantly lower in group B than in group A at 1 and 2 weeks ( P<0.05). The expression of miR-21 was significantly lower in group D than in group C at 1 and 2 weeks ( P<0.05). The expression of PTEN protein was significantly lower in group C than in groups A and D ( P<0.05). The mRNA expressions of Runx2, OPN, and Col1a1 were significantly lower in group D than in group C at 1 and 2 weeks ( P<0.05). The extracellular calcium deposition in group D was obviously less than that in group C. The expression of PTEN protein was significantly higher in group C1 than in group D1 ( P<0.05). The mRNA expressions of Runx2 and OPN were significantly lower in group C1 than in groups B1 and D1 at 1 and 2 weeks ( P<0.05). The mRNA expression of Col1a1 was significantly lower in group C1 than in groups B1 and D1 at 2 weeks ( P<0.05). The extracellular calcium deposition in group C1 was obviously less than those in groups B1 and D1. The mRNA expressions of OPN and Col1a1 were significantly higher in group D2 than in groups B2 and C2 at 1 week ( P<0.05). The extracellular calcium deposition in group D2 was obviously more than those in groups B2 and C2. H 2O 2 inhibits the osteogenic differentiation of MC3T3-E1 cells, which may be induced by down-regulating the expression of miR-21.

ANALYSIS OF BIOACTIVE COMPOUNDS AND MORPHOLOGICAL TRAITS IN INDONESIAN RODENT TUBER MUTANT CLONES OF PEKALONGAN ACCESSION USING GC-MS

Rodent tuber (Typhonium flagelliforme Lodd.) is one of the considerable potential Indonesian medicinal plants. There are many origins of rodent tuber plants in Indonesia such as Pekalongan. Increasing genetic diversity of rodent tuber’s Pekalongan accession had been done by using gamma irradiation. Its produced 23 clones of the 7th generation of mutant plants. The focus of this study was on the analysis of biomass and bioactive compounds from those clones using GC-MS. The aim of this study was to determine new bioactive compounds which affect as anticancer in the 7th mutant generation of rodent tuber plant Pekalongan accession. The result showed that 8 mutant clones of rodent tuber had higher biomass than the control. 10 bioactive compounds were detected in the leaves from 7 mutant clones (20-1-1-2-2-3, 20-1-2-2-1-3, 20-1-2-1-2-2, 20-1-2-4-5, 20-1-1-1-3, 20-4-2-1-1-1, 20-1-2-1-2-6) and 7 bioactive compounds in the tubers from 9 mutant clones (20-1-1-2-2-3, 20-1-2-2-1-3, 20-1-2-1-2-2, 20-1-2-4-5, 20-1-1-1-3, 20-4-2-1-1-1, 20-1-2-1-2-6, 20-1-2-2-4-3, 20-1-2-2-3). The bioactive compounds in the mutant clones was 4 times greater than the control. Phytol isomer and eicosane were found as new bioactive compounds in the leaves. 5 new bioactive compounds were found in the tubers for example Hexadecanoic acid ethyl ester, octadecadienoic acid, squalene, beta, eicosane and octacosane. Gamma irradiation is an effective technique to increase the huge numbers of bioactive compounds which can be applied as anticancer, antitumor and antimicobial herbal medicine.

Molecular dynamics simulations of single siloxane dendrimers: Molecular structure and intramolecular mobility of terminal groups.

Molecular dynamics simulations of two types of isolated siloxane dendrimers of various generations (from the 2nd to the 8th) have been performed for temperatures ranging from 150 K to 600 K. The first type of dendrimer molecules has short spacers consisting of a single oxygen atom. In the dendrimers of the second type, spacers are longer and comprised of two oxygen atoms separated by a single silicon atom. A comparative analysis of molecular macroscopic parameters such as the gyration radius and the shape factor as well as atom distributions within dendrimer interior has been performed for varying generation number, temperature, and spacer length. It has been found that the short-spacer dendrimers of the 7th and 8th generations have a stressed central part with elongated bonds and deformed valence angles. Investigation of the time evolution of radial displacements of the terminal Si atoms has shown that a fraction of the Si groups have a reduced mobility. Therefore, rather long time trajectories (of the order of tens of nanoseconds) are required to study dendrimer intramolecular dynamics.

Demonetization and cashless economy

Since the midnight of November 8’Th 2016 suddenly people of India faced a major currency crunch by finding Rs 500 & 1000 notes irrelevant for payment purposes. Since then the issue of India making less on cash dependent came into being. For a common man the term ‘cashless’ may sound as if India is going to become more rich i.e. by using less cash they can get more. But, certainly it is not so --Neither it is for the cash abundant people to use less-cash nor a move towards barter economy rather to transform India into relatively better economy. Now the Global economy has moved from the state of Star war to Digital war in which China has taken a lead over America by having biggest On-Line payments system and fastest computers. We are marching on 3G & 4 G their common people are running on 7th generation services. So the crux of the issue is how to make Indians to switch over to use more of electronic mode of payments even without changing the other economic parameters. We generally correlate level of education & adoption of latest technology, income status & and adoption of technology. But in this present although very short study we found that with respect to mode of electronic payment mechanism formal sector is significantly weak from demand side while informal sector is from both demand as well as supply side.

Breeding of carnations (Dianthus caryophyllus L.) for long vase life.

Carnation (Dianthus caryophyllus L.) is one of the main floricultural crops in Japan and worldwide. The vase life of cut ornamental flowers, including carnations, is important in determining their quality and consumers’ preference. To improve the vase life of carnation flowers, my group started a breeding research program in 1992 using conventional cross-breeding techniques. We repeatedly crossed and selected promising offspring with long vase life for seven generations, from 1992 to 2008. In 2005, we developed two cultivars, ‘Miracle Rouge’ and ‘Miracle Symphony’, with genetically determined long vase lives of 17.7 to 20.7 days (3.2 to 3.6 times that of ‘White Sim’) under standard conditions (23°C, 70% RH, 12-h photoperiod). Line 532-6 showed an ultra-long vase life averaging 27.8 to 32.7 days (4.6 to 5.4 times that of ‘White Sim’). We evaluated changes in ethylene sensitivity with flower senescence simply and accurately using a time-lapse video recorder. In 2010, we selected line 806-46b with both ultra-long vase life (27.1 days, 4.4 times that of ‘White Sim’) and ethylene resistance. Analyses using six cultivars and 123 selected lines from the 1st to the 7th generations revealed that the long vase life was strongly associated with a decrease in ethylene production.

Оценка проходимости синтетических кондуитов из пористого политетрафторэтилена при реконструкции магистральных вен (экспериментальное исследование)

Цель - изучение проходимости синтетических кондуитов из пористого политетрафторэтилена при реконструкции магистральных вен и обоснование их применения в клинической практике. Методика. Исследование выполнено на 70 кроликах половозрелого возраста мужского пола породы «Белый новозеландский» массой 3,0-3,5 кг. 40 кроликам выполнено линейное протезирование инфраренального отдела задней полой вены. 30 кроликам выполнено линейное протезирование инфраренального отдела брюшной аорты (группа сравнения). Оперативные вмешательства были выполнены в асептических условиях под внутримышечным наркозом. Для протезирования брюшной аорты и задней полой вены использовали политетрафторэтиленовые протезы (7 поколение, 2010) производства ЗАО «НПК «Экофлон» (Россия) с внутренним диаметром 4,0 мм и длиной 20,0 мм. Все анастомозы формировали атравматическим шовным материалом 7/0 или 8/0 с использованием микроинструментов. На протяжении всего срока эксперимента антикоагулянтной и антиагрегантной терапии не проводилось. В течение всего срока наблюдения контроль проходимости протезов осуществляли ультразвуковой допплерографией скоростных показателей кровотока дистальнее и проксимальнее протеза на сроках 3, 10, 30, 90, 180, 270 сут. На контрольных сроках наблюдения проходимость протеза оценивали прямым инвазивным измерением давления крови, а также интраоперационной ультразвуковой допплерографией линейной скорости кровотока дистальнее и проксимальнее протеза. После вывода животного из эксперимента протез иссекали с окружающими тканями для дальнейшего изучения. При сравнении показателей давления крови и линейной скорости кровотока дистальнее и проксимальнее протеза на разных сроках использовали критерий Манна-Уитни. Критический уровень значимости при проверке статистических гипотез принимался &lt;0,05. Результаты. Статистически значимой разницы линейных скоростей кровотоков и давления крови проксимальнее и дистальнее протеза на всех сроках наблюдения не выявлено. Проходимость протезов из политетрафторэтилена в венозной позиции не отличается от таковой в артериальной позиции. Тромбозов и гемодинамически значимых стенозов синтетических кондуитов из пористого политетрафторэтилена, как в венозной, так и в артериальной позиции не отмечено на всех сроках. Заключение. Проходимость синтетических кондуитов из пористого политетрафторэтилена в венозной позиции сравнима и не отличается от таковой в артериальной позиции. Полученные данные позволяют получить экспериментальное обоснование возможности использования синтетических кондуитов из пористого политетрафторэтилена при реконструкции магистральных вен в клинической практике. Aim. To study patency of synthetic, porous polytetrafluoroethylene (PTFE) conduits in reconstruction of great veins and to justify their use in clinical practice. Methods. The study was conducted on 70 New Zealand male rabbits weighing 3.0-3.5 kg. Infrarenal linear prosthetic reconstruction of posterior vena cava was performed in 40 rabbits. Infrarenal linear prosthetic reconstruction of abdominal aorta was performed in 30 rabbits (control group). All surgical procedures were conducted in aseptic conditions under intramuscular anesthesia. Porous PTFE conduits with 4 mm internal diameter and 20 mm length (7th generation, 2010; ZAO NPK Ecoflon, Russia) were used for prostheses of aorta and posterior vena cava. All anastomoses were made of atraumatic 7/0-8/0 ligature using microsurgical instruments. No anticoagulant therapy was used throughout the experimental period. During the study, conduit patency was controlled by ultrasound Doppler monitoring of blood flow velocity distal and proximal to the conduit and direct, invasive BP measurements during the surgery and at 3, 10, 30, 90, 180, and 270 days of surgery. At the end of experiment, the conduit was removed from the animal together with adjacent structures. The Mann-Whitney U-test was used for comparison of BP and blood flow velocity distal and proximal to the conduit. Differences were considered significant at p ?0.05. Results. Significant differences between values of linear blood flow velocity and BP distal and proximal to the conduit were absent in the entire follow up period. The patency of porous PTFE conduits was similar in both arterial and venous positions. No conduit thrombosis or hemodynamically significant stenosis were observed in arterial or venous positions in the entire follow up period. Conclusion. The patency of PTFE conduits in the venous position is similar to and not different from the arterial position. The obtained experimental data support the use of synthetic PTFE conduits for reconstruction of great veins in clinical practice. Conclusion. The polytetrafluoroethylene conduits patency of in venous position is similar and does not differ from arterial position. Obtained experimental data gives the opportunity for synthetic polytetrafluoroethylene conduits appliance for main vein reconstruction in clinical practice.

Deposition of glass fibers in a physically realistic replica of the human respiratory tract

Regional deposition of glass fibers was investigated in a physically realistic, human respiratory tract replica. The replica begins with the oral cavity and includes the airways up to the 7th generation of the tracheobronchial tree. Uniform diameter glass fibers were classified by length using a dielectrophoretic classifier and introduced into the replica at three steady-state flow rates (15, 30, and 50 LPM). A novel automatic image processing method was utilized to speed up the sample analysis and make it more reproducible. Fractional deposition was high in the oral cavity and the upper respiratory airways. Deposition density was higher in the first few generations of the tracheobronchial tree. Deposition efficiencies were compared with published data and good agreement was obtained. Our data confirmed that the deposition efficiency increased with increasing Stokes number indicating that impaction was the main deposition mechanism. The experimental data were used to propose new empirical models predicting fiber deposition in the tracheobronchial tree.

Airway morphology and inspiratory flow features in the early stages of Chronic Obstructive Pulmonary Disease

BackgroundChronic Obstructive Pulmonary Disease (COPD) is among the leading causes of death worldwide. Inhaled pollutants are the prime risk factor, but the pathogenesis and progression of the diseased is poorly understood. Most studies on the disease onset and trajectory have focused on genetic and molecular biomarkers. Here we investigate the role of the airway anatomy and the consequent respiratory fluid mechanics on the development of COPD. MethodsWe segmented CT scans from a five-year longitudinal study in three groups of smokers (18 subjects each) having: (i) minimal/mild obstruction at baseline with declining lung function at year five; (ii) minimal/mild obstruction at baseline with stable function, and (iii) normal and stable lung function over the five year period. We reconstructed the bronchial trees up to the 7th generation, and for one subject in each group we performed MRI velocimetry in 3D printed models. FindingsThe subjects with airflow obstruction at baseline have smaller airway diameters, smaller child-to-parent diameter ratios, larger length-to-diameter ratios, and smaller fractal dimensions. The differences are more significant for subjects that develop severe decline in pulmonary function. The secondary flows that characterize lateral dispersion along the airways are found to be less intense in the subjects with airflow obstruction. InterpretationThese results indicate that morphology of the conducting airways and inspiratory flow features are correlated with the status and progression of COPD already at an early stage of the disease. This suggests that imaging-based biomarkers may allow a pre-symptomatic diagnosis of disease progression.