Sec Delay Research Articles

The influence of retrieval on retention.

Four experiments tested the hypothesis that successful retrieval of an item from memory affects retention only because the retrieval provides an additional presentation of the target item. Two methods of learning paired associates were compared. In the pure study trial (pure ST condition) method, both items of a pair were presented simultaneously for study. In the test trial/study trial (TTST condition) method, subjects attempted to retrieve the response term during a period in which only the stimulus term was present (and the response term of the pair was presented after a 5-sec delay). Final retention of target items was tested with cued-recall tests. In Experiment 1, there was a reliable advantage in final testing for nonsense-syllable/number pairs in the TTST condition over pairs in the pure ST condition. In Experiment 2, the same result was obtained with Eskimo/English word pairs. This benefit of the TTST condition was not apparently different for final retrieval after 5 min or after 24 h. Experiments 3 and 4 ruled out two artifactual explanations of the TTST advantage observed in the first two experiments. Because performing a memory retrieval (TTST condition) led to better performance than pure study (pure ST condition), the results reject the hypothesis that a successful retrieval is beneficial only to the extent that it provides another study experience.

A simple T-maze method for estimating working memory in mice effect of ethylcholine mustard aziridinium ion (AF64A)

Mice were housed in a cage containing a T maze. A watering place was located at the entrance of the maze. The right and left arms of the maze each had two exits, one of which led to the home cage where food was placed, while the other led to the watering place via a bypass. The exit leading to the home cage in either the right or left arm was alternately closed every 90 min. One-way swinging doors were inserted at the entrance arm and between each bypass and the watering place. The mice were given a cholinergic neurotoxin—ethylcholine mustard aziridinium ion (AF64A)—(8 nmol) or saline as a control into the left ventricle 2 weeks before they were housed in the apparatus. Those mice housed in this apparatus mastered the alternation task at a 5-sec delay on day 3 in the sham group and on day 4 in the AF64A group. When a longer delay (5–90 sec) was introduced for the mice that mastered the alternation task at 5-sec delay, the AF64A group made significantly more errors than did the sham group at 60- and 90-sec delays. These results show that the apparatus is useful in estimating working memory in mice with little effort.

Facilitation of Working Memory in Humans by a D2 Dopamine Receptor Agonist

Recent studies on the neurobiology of cognition have focused on the ability of the prefrontal cortex (PFC) to support processes of working memory, i.e, mnemonic processes by which information relevant for a correct response is temporarily maintained to be reevaluated or updated on a trial-by-trial basis. Of most recent interest is the role played by dopamine (DA) in spatial working memory processes of the principal sulcal region of the PFC. Although D1 DA receptors appear to modulate these mnemonic processes in monkeys, several lines of research suggest that D2 DA receptors could also be relevant to cognitive functions. Therefore, we assessed the effects of a specific D2 receptor agonist (bromocriptine) and placebo on visuospatial delayed response performance in human subjects. During delay periods of 0 or 8 sec, subjects were required to remember the spatial location of rapidly presented visual cues displayed in peripheral vision within a 360° circumference. The extent to which D2 receptor activation by bromocriptine facilitated working memory in the 8-sec delay condition relative to placebo performance was assessed. As a means of providing validation of bromocriptine's D2 receptor effect, maximum inhibition of prolactin (PRL) secretion, which is inhibited specifically by activation of D2 receptor sites, was determined. Additionally, tasks having no working memory component were administered to rule out nonspecific effects of bromocriptine on sensory, arousal, attentional, and motor factors. Results demonstrated a significant facilitatory effect of bromocriptine on spatial delayed response performance (i.e., 8-sec delay performance). Results could not be explained by nonspecific effects of bromocriptine. Thus, findings of this study suggest that spatial working memory is facilitated by D2 receptor activation. The role that DA may play in human cognitive processes is discussed within the larger theoretical framework of DA's general role in the facilitation of goal-directed behavior. In the case of cognition, DA may facilitate processes that serve to guide motivated behavior through complex environments.

Analysis of alpha-2 adrenergic agonist effects on the delayed nonmatch-to-sample performance of aged rhesus monkeys

The administration of alpha-2 adrenergic agonists to aged monkeys has been shown to ameliorate their cognitive deficits on the delayed response (DR) task, a test of spatial working memory (3,5). The present experiment tested whether the alpha-2 agonists, clonidine and guanfacine, would also improve working memory for object feature recognition, as tested by the delayed nonmatch-to-sample (DNMS) task. Five aged monkeys were trained on DNMS and were found to have mild performance deficits comparable to those reported previously for monkeys of similar age (32). However, during the subsequent two years of drug testing, the animals' baseline performance steadily improved, and conditions had to be made progressively more difficult to produce errors in performance. Clonidine and guanfacine significantly altered the DNMS performance of the aged monkeys, but drug-induced improvement was not as robust for DNMS as it was for DR. Clonidine produced a triphasic dose/response curve: Impairment was observed at both very low and high doses, while modest improvement was seen in the middle dose range (average maximal improvement of 21 ± 2.4%). Although improvement could occasionally be replicated for some doses, the clonidine dose/response curves were remarkably inconsistent in the middle dose range. Similarly, doses of guanfacine which had previously produced optimal improvement on the DR task, produced only small but significant improvement in DNMS performance (average improvement of 11 ± 3% for the 0.00011–0.000011 mg/kg dose range). By the end of the drug study, three monkeys were performing well (70–75% correct) with lists of 20 objects and over 400 sec delays, and one monkey performed better than 85% correct with lists of 40 objects and over 800 sec delays. Given the high level of performance of the aged monkeys on the DNMS task, it is unclear whether the weak improvement produced by alpha-2 agonists on the DNMS as compared to the DR task is due to ceiling effects, or rather results from alpha-2 adrenergic mechanisms being more important for spatial working memory than memory for object features. Future experiments utilizing a more difficult object working memory task will be necessary to resolve this issue.

Rupture process of the 1987 Superstition Hills earthquake from the inversion of strong-motion data

Abstract A pair of significant earthquakes occurred on conjugate faults in the western Imperial Valley involving the through-going Superstition Hills fault and the Elmore Ranch cross fault. The first event was located on the Elmore Ranch fault, Ms = 6.2, and the larger event on the Superstition Hills fault, Ms = 6.6. The latter event is seen as a doublet teleseismically with the amplitudes in the ratio of 1:2 and delayed by about 8 sec. This 8-sec delay is also seen in about a dozen strong-motion records. These strong-motion records are used in a constrained least-squares inversion scheme to determine the distribution of slip on a 2-D fault. Upon closer examination, the first of the doublets was found to be itself complex requiring two episodes of slip. Thus, the rupture model was allowed to have three separate subevents, treated as separate ruptures, with independent locations and start times. The best fits were obtained when all three events initiated at the northwestern end of the fault near the intersection of the cross-fault. Their respective delays are 2.1 and 8.6 sec relative to the first subevent, and their moments are 0.4, 0.9, and 3.5 × 1025 dyne-cm, which is about half of that seen teleseismically. This slip distribution suggests multi-rupturing of a single asperity with stress drops of 60, 200, and 15 bars, respectively. The first two subevents were confined to a small area around the epicenter while the third propagated 18 km southwestward, compatible with the teleseismic and afterslip observations.

Electronic determination of size and number in isolated unfixed adipocyte populations

Adipose tissue cellularity and metabolism are traditionally expressed in terms of mean cell size and number. The need for a simple method allowing rapid determination of cell size and number of freshly isolated, unfixed adipocyte preparations led us to compare estimates of cell size determined by the established method of optical sizing to a proposed method of electronic cell sizing and counting. In collagenase-isolated, unfixed adipocytes whose mean diameters ranged from approximately 40 to 65 microns (obtained from healthy rats weighing 100-360 g) the electronic method provided estimates of the mean cell diameter and size distribution that did not differ from the optical sizing technique. Estimates of mean cell diameter and cell number by the electronic method were rapid and reproducible (coefficients of variation 0.5 and 3.8%, respectively) and a less than 20 sec delay until sample analysis, after mixing of the adipocyte suspension, did not alter these estimates. Electronic determination of cell size and number, using freshly isolated, unfixed rat adipocyte populations (mean cell diameter less than or equal to 60 microns), is rapid and reliable. It will be particularly useful for studies of hormone binding and transport processes where it may be necessary to tightly control cell density.

Short-term memory for spatial location in goal-directed locomotion

A blind walking study was conducted to examine the nature of the representation that subjects use to guide goal-directed locomotion. Subjects walked blindfolded to targets 4,8, and 12 m away, either immediately after visual occlusion or following a 2-sec or 30-sec no-vision delay. On half of the trials, subjects were cued as to target prior to visual occlusion; on the other trials, the specific target was identified after visual occlusion, just prior to walking. Although consistency in target acquisition was influenced by both distance and delay, prior knowledge as to target had no impact on error. These results suggest that subjects remember/forget the general spatial layout of the task environment as opposed to a specific target location.

A study of transient processes in hypothetical accidents in a fast critical assembly with a moderating reflector

1. The algorithm for the integration of the reactor kinetics equations, based on Gear's numerical methods, provides an efficient means of calculating critical-assembly accidents, in which the assembly rapidly goes into prompt-neutron supercriticality and then slowly cools down. 2. The maximum temperature in the assembly zones, and the energy released during the burst, are not appreciably affected by the positive reactivity insertion rate. 3. Accident damage is significantly reduced (the assembly remains operational) only in cases of rapid scram-rod dumping (≤0.3 sec delay), provided the temperature coefficient of reactivity equals or exceeds the rated value.

Effects of [D-Ala 2] Met-enkephalinamide, a Met-enkephalin analog, on delayed response by squirrel monkeys

Squirrel monkeys were tested on an indirect spatial delayed response task after subcutaneous injections of either physiological saline, 100, or 500 microgram/kg [D-Ala 2] methionine enkephalinamide (DAME). During Experiment 1 monkeys completed two-choice delayed response problems with 0-, 6-, 12- and 18- sec delays; DAME significantly enhanced performance at 18-sec delays but not for the shorter delay. During Experiment 2, monkeys performed a nine-choice spatial delayed response task with 0-, 4- and 8-sec delays. In Experiment 2, low but not high doses of DAME impaired performance. Memory functions represented by a bias-free performance measure paralleled percentage correct in both experiments. Therefore, DAME effects, in enhancing and in impairing performance, were not attributable to changes in the relative number of systematic spatial errors (positional win-shift; lose-stay, position perseveration, and position preference).

Pigeons’ memory for event duration: Intertrial interval and delay effects

The effects of within-session variations in the intertriai interval (ITI) and delay on pigeons’ memory for event duration were studied in delayed symbolic matching-to-sample tasks. Pigeons were trained to peck one color following a long (8 sec) sample and another color following a short (2 sec) sample. In the first three experiments, the baseline conditions included a 10-sec delay (retention interval) and a 45-sec ITI. During testing, the delay was varied from 0 to 20 sec, and the ITI that preceded the trial was varied from 5 to 90 sec. When the ITI and delay were manipulated separately (Experiments 1 and 2), the pigeons displayed a choose-short tendency when the delay was longer than 10 sec or when the ITI was longer than 45 sec, and a choose-long tendency when either the delay or the ITI was shorter than these baseline values. These effects occurred whether the sample was food access or light. When the ITI and delay were manipulated together, the pigeons showed a large choose-long error tendency when the short delay was tested together with a short ITI, and no systematic error tendency when the short delay was tested together with a longer ITI. A very large choose-short error tendency emerged on trials with a long delay and a long ITI; a reduced choose-short tendency was present when the long delay was presented together with a short ITI. In Experiment 4, the baseline conditions were a 0-sec delay and a 45-sec ITI. In this case variations in the ITI had a smaller and unidirectional effect: the pigeons showed a choose-long error tendency when the ITI was decreased, but no effect of ITI increases. Two hypotheses were proposed and discussed: (1) that pigeons judge sample durations relative to a background time composed of the ITI and delay, and (2) that the delay and ITI effects might arise from a combination of subjective shortening and proactive effects of samples from previous trials.

Molindone: Effects in pigeons responding under conditional discrimination tasks

Pigeons were trained to respond under three conditional discrimination procedures; 1) a fixed-consecutive-number procedure with (FCN 8-SD) and without (FCN 8) an added external discriminative stimulus, 2) a delayed matching-to-sample (DMTS) procedure using 0-sec, 2-sec and 8-sec delay intervals, and 3) a repeated acquisition of behavioral chains (RA) procedure using a four-link response chain with three stimulus keys. The atypical neuroleleptic agent molindone decreased accuracy under the FCN 8 at doses that had no effect on accuracy under the FCN 8-SD. Under the DMTS procedure, molindone-induced decreases in accuracy were directly related to the delay interval, with the largest relative decrements obtained at the 8-sec delay and the smallest at the 0-sec delay. Under the RA procedure, molindone decreased accuracy at doses that had little or no effect on the number of correct responses emitted. Relative to control values, molindone-induced decreases in accuracy were smallest under the DMTS and FCN 8-SD procedures and largest under the FCN 8 and RA procedures. The differential effects obtained with molindone under each of these procedures illustrate the need to employ a variety of assays when determining the behavioral actions of neuroleptics. In addition, this battery of behavioral tests may provide a useful tool for assessing the different neurochemical actions of neuroleptic compounds.

Differential effects of neuroleptic drugs on the delayed matching-to-sample performance of pigeons

The effects of clozapine, thiothixene, sulpiride, chlorpromazine and loxapine were examined in pigeons responding under a delayed matching-to-sample (DMTS) procedure using 0-, 2- and 8-sec delay intervals. Chlorpromazine (3–100 mg/kg), thiothixene (0.03–1.7 mg/kg), clozapine (0.1–5.6 mg/kg) and loxapine (0.1–10 mg/kg) produced dose-related decreases in the percent of correct responses (accuracy). With the exception of chlorpromazine, the relative magnitude of the accuracy-decreasing effects were unrelated to the length of the delay interval and the nondrug levels of accuracy. In contrast to these accuracy-decreasing effects, sulpiride (3–300 mg/kg) failed to decrease accuracy across the range of doses evaluated. Chlorpromazine, loxapine and clozapine increased responses rates at low doses and then decreased responses rates as the dose was increased. Thiothixene and sulpiride only decreased response rates in a dose-dependent fashion. The order of potency for the rate-suppressing effects of these drugs was thiothixene > clozapine = loxapine > chlorpromazine > sulpiride. The results of the present investigation suggest that, despite similar dopamine antagonist properties, neuroleptics produce qualitatively different effects in pigeons responding under DMTS procedures.

Delayed matching of visual materials by a bottlenosed dolphin aided by auditory symbols

A bottlenosed dolphin (Tursiops truncatus) with good underwater and aerial visual acuity was tested in visual matching-to-sample (MTS) paradigms. Attempts to train visual identity MTS directly, using two simple two-dimensional patterns as sample stimuli and as alternatives (comparison stimuli), met with little success, in keeping with previously observed difficulties of this auditory-specialized species for learning complex tasks utilizing simple visual materials. Pairing of each visual sample with a unique sound, to produce a compound auditory-visual sample, while retaining the two visual alternatives, resulted in the dolphin’s learning both auditory-visual symbolic matching and visual-visual identity matching. At 0-sec delay, performance with the auditory element of the sample alone was equivalent (76%) to performance with the visual element alone; performance with the compound was distinctly better (95%–98% correct). Testing with longer delays using the visual element alone resulted in successful matching through to a maximum delay of 34 sec. These results provided the first demonstration of delayed MTS in a dolphin using visual materials, and complemented other data showing the ready capability of this species for delayedauditory MTS. It appeared that the dolphin solved the visual MTS task by forming auditory codes to represent the visual materials, and that these auditory codes were eventually replaced with purely visual codes.

Lifetime low-level lead exposure produces deficits in delayed alternation in adult monkeys

Cynomolgus monkeys ( Macaca fascicularis) were dosed continuously from birth onward with 100, 50, or 0 μg/kg/day of lead. This resulted in blood lead concentrations of 25, 15, or 3 μg/dl respectively before withdrawal of infant formula at 200 days of age. Blood lead concentrations declined thereafter over the next 100–150 days to steady-state concentrations of 13, 11, or 3 μg/dl. At seven to eight years of age, monkeys were tested on a delayed alternation task. The task required the monkey to alternate responses between two pushbuttons; each alternation was rewarded with a small amount of apple juice. After each monkey learned the task, a delay was instituted between trials. The initial delay was 100 msec, and was increased in steps to 15 sec by the end of the experiment. Treated monkeys were impaired in their ability to learn the alternation task, but were not different from controls at short delay values (1 and 3 sec). At longer delay values (5 and 15 sec), treated monkeys again exhibited impairment. At the 15 sec delay value, some individuals in both treated groups exhibited marked perseveration, responding on the same button in some instances for hours at a time. Treated monkeys were also more variable in their performance across sessions than were controls. The data are interpreted as indicative of spatial learning and short-term memory deficits in the lead-exposed monkeys.

Evoked potential estimates of the time course of adaptation and recovery to counterphase gratings

Scalp-recorded evoked potentials (VEP) were sequentially sampled in humans during adaptation to and recovery from prolonged viewing of counterphase sinusoidal grating targets. The sum of the power at the first and second harmonics of the Fourier-transformed VEP components was found to decrease during adaptation and increase during recovery. Time constants ( T) for the adaptation and recovery processes as estimated from exponential functions ranged from 2.9 to 19 sec, varying nonmonotonically with the spatial frequency and contrast of the stimulus. The observed T values are shorter than those reported in psychophysical studies of adaptation but overlap estimates derived from single cell studies. An unexpected finding was the occurrence of a 3–6 sec delay in the appearance of the maximum VEP response after the onset of the adaptation stimulus. The delay occurred in all subjects and at all spatial frequencies when moderate to high adapting contrasts (e.g. > 0.2) were used. The data support a feature-selective, multi-channel lateral inhibitory model of spatial vision and suggest the presence of tonic inhibition between the channels.

Effects of scopolamine on performance of rats in a delayed-response radial maze task

The effects of the cholinergic blocker scopolamine on performance of rats in an 8-arm radial maze were studied. In Experiment 1, rats received injections of scopolamine-HBr (0.2 mg/kg, IP) or saline, 20 min before a trial. The drug impaired performance only when midtrial delays were introduced (10 sec, 5, 20, 60 or 120 min), during which the animals were removed from the apparatus, and to a similar extent at all delays. Injection of scopolamine directly after choice 4 in a 20 min delayed trial affected performance at a dose of 0.4 mg/kg, but not at 0.2 mg/kg. In Experiment 2, the rats were kept in the apparatus during the delay. There was a significant effect of scopolamine at 0.1 and at 0.2 mg/kg, initially irrespective of the length of the delay (10 sec, 2.5 or 5 min). After considerable training, administration of scopolamine (0.2 mg/kg) had no significant effect at the 10 sec delay but did impair performance after longer (5 or 10 min) midtrial intervals. These results suggest that scopolamine has differential effects, depending on dose and degree of training, and that an effect on memory storage may be one of them.

Effects of selection delays on radial maze performance: Acquisition and effects of scopolamine

The effects of post-selection confinement (delays) on both the acquisition of performance and the response to the muscarinic blocker, scopolamine, were examined in an automated version of the eight arm radial maze. Long-Evans rats, exposed to post-selection delays of 0.5 sec (n = 4) or 100 sec (n = 4) during daily training trials did not differ in either the number of trials to acquire an accurate baseline of performace or in the amount of time required to obtain all eight food pellets. However, the pattern (delta-arm scores) of within-session arm selections demonstrated by the two groups of rats differed. Rats exposed to the 0.5 sec delay typically selected arms adjacent to arms from which they exited while rats exposed to the 100-sec delay were more likely to enter arms 2-removed from the exit arm. When scopolamine (0.03 to 1.0 mg/kg) was administered prior to testing, rats in the 100-sec delay group showed a greater reduction of accuracy and a larger increase in selection latency than rats in the 0.5-sec delay group. The differential effect of delay value on delta-arm scores was also eliminated in a dosage dependent manner with scopolamine. Schopolamine methylbromide (0.3 mg/kg) was found to have little effect on performance. In summary, the results indicate that the post-selection delay procedure is a sensitive and selective test for chemical-induced dysfunctioning of spatial memory in rats.

Reversal of a trimethyltin-induced learning deficit by desglycinamide-8-arginine vasopressin

Trimethyltin (TMT) is an organometal neurotoxin which produces lesions primarily in the limbic system. Selectivity seems to depend upon the dose, but the hippocampus and related entorhinal cortical structures, of importance for learning and memory, are most often described as target sites. We have previously demonstrated that subjects treated with a moderate dose of TMT prior to acquisition sessions, are unable to learn a forward autoshaping task with a 6 sec delay of reinforcement, but are capable of acquiring the same task when no delay of reinforcement is used. These data suggested that the performance deficit is one of learning (i.e. consolidation) rather than of memory (i.e. storage), retrieval, or sensorimotor impairment. To more rigorously test this hypothesis, we determined if performance of a task already learned would be impaired by the neurotoxin. Adult male Long Evans rats were given 10 acquisition sessions of 24 trials, following which TMT (6.0 mg/kg, p.o.) was administered. One month later, these rats performed the lever-touching behavior as well as controls, despite the fact that the same dose of TMT interfered with learning if given one month prior to acquisition sessions, thus confirming our hypothesis. In a second experiment we determined if the peptide analog of vasopressin, desglycinamide-8-arginine vasopressin (DGAVP), could reverse a learning deficit in a population of non-learners. Rats were treated with TMT or water vehicle one month prior to autoshaping. TMT significantly retarded acquisition. After 10 sessions of 12 trials each, non-learners (i.e. rats treated with TMT that failed to associate the lever with delivery of a reinforcer) were administered saline or DGAVP (7.5 ug/kg, s.c.) 1 hr before sessions 11–13; treatment was discontinued prior to sessions 14 and 15. Peptide treated subjects showed evidence of acquisition and exhibited higher levels of lever-directed behavior than saline treated nonlearners. Performance was maintained after DGAVP treatment was discontinued, indicating that the learning-enhancing action of DGAVP was not transient or state-dependent.

Rats’ auditory working memory tested by continuous non-matching-to-sample performance

Rats were trained in a continuous non-matching-to-sample task in order to study the retention of auditory information over a brief delay interval. A 4-sec delay period (retention interval) was imposed between trials. The subjects acquired the task with approximately 1 month of training. Subsequent changes in length of tone presentations and length of the delay period showed that response accuracy decreased with prolongation of the delay period, but did not vary as a function of tone duration. This suggests that the decrement of response accuracy with increases in delay period was due to memory decay, not to prolongation of the interval between response opportunities. Continuous non-matching-to-sample may be a useful paradigm for studying working memory, and some potential applications of the procedure are discussed.

Fast fracture of rubber-toughened thermoplastics used for the shells of motorcycle helmets

The effects of outdoor ageing and of accelerated UV exposure on the grade of acrylonitrile butadiene styrene copolymer (ABS) used by UK manufacturers of motorcycle helmets were compared. Although the surface layer becomes embrittled the helmets still survive an impact test after conditioning at -20° C because the cracks are arrested before they penetrate the full thickness of the shell. A 4 mm thick rubber-toughened polycarbonate, used in helmets from continental suppliers, shows a transition from “tough” to “brittle” crack propagation behaviour at -15° C. For 4 mm thick ABS this transition had not occurred at the lowest test temperature of -20° C, but if 6 mm sheet is tested the transition is at -15° C. Temperature increases in the 40 sec delay between conditioning a helmet at -20° C and impact testing are shown to be significant.