4th Generation Research Articles

Integration of epitaxial LiNbO3 thin films with silicon technology

Development of bulk acoustic wave filters with ultra-wide pass bands and operating at high frequencies for 5th and 6th generation telecommunication applications and micro-scale actuators, energy harvesters and sensors requires lead-free piezoelectric thin films with high electromechanical coupling and compatible with Si technology. In this paper, the epitaxial growth of 36°Y-X and 30°X-Y LiNbO3 films by direct liquid injection chemical vapour deposition on Si substrates by using epitaxial SrTiO3 layers, grown by molecular beam epitaxy, has been demonstrated. The stability of the interfaces and chemical interactions between SrTiO3, LiNbO3 and Si were studied experimentally and by thermodynamical calculations. The experimental conditions for pure 36°Y-X orientation growth have been optimized. The piezoelectricity of epitaxial 36°Y-X LiNbO3/SrTiO3/Si films was confirmed by means of piezoelectric force microscopy measurements and the ferroelectric domain inversion was attained at 85 kV.cm−1 as expected for the nearly stoichiometric LiNbO3. According to the theoretical calculations, 36°Y-X LiNbO3 films on Si could offer an electromechanical coupling of 24.4% for thickness extension excitation of bulk acoustic waves and a comparable figure of merit of actuators and vibrational energy harvesters to that of standard PbZr1−x Ti x O3 films.

In vitro toxicological evaluation of aerosols generated by a 4th generation vaping device using nicotine salts in an air-liquid interface system

BackgroundElectronic cigarettes (EC) have gained popularity, especially among young people, with the introduction of fourth-generation devices based on e-liquids containing nicotine salts that promise a smoother vaping experience than freebase nicotine. However, the toxicological effects of nicotine salts are still largely unknown, and the chemical diversity of e-liquids limits the comparison between different studies to determine the contribution of each compound to the cytotoxicity of EC aerosols. Therefore, the aim of this study was to evaluate the toxicological profile of controlled composition e-liquid aerosols to accurately determine the effects of each ingredient based on exposure at the air-liquid interface.MethodsHuman lung epithelial cells (A549) were exposed to undiluted aerosols of controlled composition e-liquids containing various ratios of propylene glycol (PG)/vegetable glycerin (VG) solvents, freebase nicotine, organic acids, nicotine salts, and flavoured commercial e-liquids. Exposure of 20 puffs was performed at the air-liquid interface following a standard vaping regimen. Toxicological outcomes, including cytotoxicity, inflammation, and oxidative stress, were assessed 24 h after exposure.ResultsPG/VG aerosols elicited a strong cytotoxic response characterised by a 50% decrease in cell viability and a 200% increase in lactate dehydrogenase (LDH) production, but had no effects on inflammation and oxidative stress. These effects occurred only at a ratio of 70/30 PG/VG, suggesting that PG is the major contributor to aerosol cytotoxicity. Both freebase nicotine and organic acids had no greater effect on cell viability and LDH release than at a 70/30 PG/VG ratio, but significantly increased inflammation and oxidative stress. Interestingly, the protonated form of nicotine in salt showed a stronger proinflammatory effect than the freebase nicotine form, while benzoic acid-based nicotine salts also induced significant oxidative stress. Flavoured commercial e-liquids was found to be cytotoxic at a threshold dose of ≈ 330 µg/cm².ConclusionOur results showed that aerosols of e-liquids consisting only of PG/VG solvents can cause severe cytotoxicity depending on the concentration of PG, while nicotine salts elicit a stronger pro-inflammatory response than freebase nicotine. Overall, aerosols from fourth-generation devices can cause different toxicological effects, the nature of which depends on the chemical composition of the e-liquid.

Effects of training flights of combat jet pilots on parameters of airway function, diffusing capacity and systemic oxidative stress, and their association with flight parameters

BackgroundFighter aircraft pilots are regularly exposed to physiological challenges from high acceleration (Gz) forces, as well as increased breathing pressure and oxygen supply in the support systems. We studied whether effects on the lung and systemic oxidative stress were detectable after real training flights comprising of a wide variety of exposure conditions, and their combinations.MethodsThirty-five pilots of the German Air Force performed 145 flights with the Eurofighter Typhoon. Prior to and after flight lung diffusing capacity for carbon monoxide (DLCO) and nitric oxide (DLNO), alveolar volume (VA), and diffusing capacities per volume (KCO, KNO) were assessed. In addition, the fractional concentration of exhaled nitric oxide (FeNO) was determined, and urine samples for the analysis of molecular species related to 8-hydroxy-2’-deoxyguanosine (8-OHdG) were taken. For statistical analysis, mixed ANOVA models were used.ResultsDLNO, DLCO, KNO, KCO and VA were reduced (p < 0.001) after flights, mean ± SD changes being 2.9 ± 5.0, 3.2 ± 5.2, 1.5 ± 3.7, 1.9 ± 3.7 and 1.4 ± 3.1%, respectively, while FeNO decreased by 11.1% and the ratio of 8-OHdG to creatinine increased by 15.7 ± 37.8%. The reductions of DLNO (DLCO) were smaller (p < 0.001) than those of KNO (KCO). In repeated flights on different days, baseline values were restored. Amongst various flight parameters comprising Gz-forces and/or being indicative of positive pressure breathing and oxygenation support, the combination of long flight duration and high altitude appeared to be linked to greater changes in DLNO and DLCO.ConclusionsThe pattern of reductions in diffusing capacities suggests effects arising from atelectasis and increased diffusion barrier, without changes in capillary blood volume. The decrease in exhaled endogenous NO suggests bronchial mucosal irritation and/or local oxidative stress, and the increase in urinary oxidized guanosine species suggests systemic oxidative stress. Although changes were small and not clinically relevant, their presence demonstrated physiological effects of real training flights in a modern 4th generation fighter jet.

Enhanced passive optical network system-based VCSEL and PSK electrical modulator for 5th generation

Enhanced passive optical network system-based VCSEL and PSK electrical modulator for 5th generation

The Occurrence, Antibiotic Susceptibility Pattern and Multiple Drug Resistance Index Studies of E. coli from Fresh Meats in Marathwada Region of Maharashtra

Background: Foodborne diseases are a huge social burden in both developed and underdeveloped countries. Escherichia coli is a natural inhabitant of the human and warm-blooded animal digestive tracts. It is considered as an indicator organism for direct or indirect faecal contamination of raw meats. The presence of antibiotic-resistant microorganisms in meat accounts for a public health risk. Methods: In current study, all 39 characterized E. coli isolates recovered on screening 405 fresh meat samples collected from different butcher shops in the Udgir city, Marathwada region of Maharashtra were also tested for Congo red dye binding assay and haemolysis assay. Antimicrobial resistance profiling of recovered isolates was carried out using the disc diffusion method. Result: Overall occurrence of E. coli in fresh meats was noted to the tune of 9.63% and the corresponding group wise occurrence in chicken and chevon samples were 12% and 7.32%, respectively. All E. coli tested positive in the Congo red dye binding assay and haemolysis assay, suggesting virulent nature of isolated organisms. On screening for antimicrobial resistance, these isolates were found resistant to mainly â-lactams (94.87%), lincosamides (84.62%), 4th Generation cephalosporins (82.05% each), DHFR Inhibitors (64.10%), glycopeptides (51.28%). The multidrug resistant isolates showed resistance to a minimum of 4 and maximum of 12 antibiotics with MAR index ranging from 0.27 to 0.8 and 9 resistance patterns.

CURRENT STATE AND DEVELOPMENT TRENDS OF E-TRADE IN UKRAINE

The article analyzes the approaches of scientists to the definition of the essence of electronic commerce, the differences between the categories "electronic commerce" and "e-trade", considers modern approaches to trade based on digital marketing. The purpose of the article is to determinatе the essence and conditions of the development of e-trade since its inception, determination of the features of the current state and development trends, advantages and disadvantages of e-trade. To realize the set goal, the following tasks were set: clarify the categorical and conceptual apparatus in the field of e-commerce and trade; determine the current state and features of e-trade in the enterprise management system; determine the key trends in the development of e-trade in the field of modern marketing concepts; determine directions of e-trade development. Certain contradictions in the international trade business led to the unification of standards in 80s, and later in the 90s there were changes in the standard, which was already based on Internet technologies. It was proved that е-trade as part of e-commerce is a source of competitive advantage for the manufacturer, seller and market partners, as it provides the possibility of remote communication with market partners, reduction of costs for the organization of the sales process, operational costs, global coverage of markets and personalized marketing. For buyers, the key advantages of online shopping are convenience, speed, a wide range of products, ease of comparison and payment. Disadvantages of e-commerce are the high intensity of competition, the risk of personal data availability and the possibility of their illegal appropriation, online fraud. The introduction of electronic commerce has significantly changed the way trade enterprises work and the possibilities of interaction with consumers. An e-trade organization removes many of the barriers of traditional trade while embracing new business principles. The differences between traditional trade and e-trade are significant in communications, business flexibility, production and sales logistics, payment methods and sales parameters: e-commerce has advantages in almost all parameters. The development of e-commerce in Ukraine in the last 5 years is taking place at a rapid pace, and this trend has only intensified as a result of the pandemic, which has developed a habitual pattern of behavior to buy online. According to the structure, the lion's share of the e-commerce market in Ukraine is the sale of electronics, toys, goods for creativity, clothes, shoes and accessories. The main trends shaping the retail industry in the perspective of 2026 are the further growth of e-commerce volumes in all regions and across all product categories, the negative impact of inflation on consumer behavior and the efficiency of retail networks; digitalization, which is becoming the main area of investment for retailers; development of the 4th generation of q-commerce (electronic commerce with an emphasis on fast delivery).

Weight Performance of 4th Generation IPB-D3 Local Chickens Aged 1-3 Months and It`s Heritability Value

Bogor Agricultural University local chicken which named IPB-D3 is a prospective male line obtained from the chicken selection program IPB-D1. The establishment of IPB-D3 chickens aims to produce local chickens with fast growth weight. This research aims to evaluate the performance of local chickens from IPB-D3 Generation 4 (G4) and its heritability values. The observed samples include 10 males and 30 females of IPB-D3 chickens of Generation 3 (G3) aged 21-40 weeks as parent and 100 Day Old Chickens (DOC) IPB-D3 G4 up to 12 weeks old. The results obtained that IPB-D3 G4 chickens show an average body weight for males 1021.44 g and females around 648.33 g at the age of 12 weeks. The feed given to IPB-D3 G4 chickens is the same as the parents, namely it has nutrients below commercial feed because the feed given uses a mixture of local feed which are bran and corn which already used in Sinar Harapan Farm Sukabumi. Weight gain is the change in body weight that occurs over a specific period, and the weight gain of IPB-D3 G4 chickens is relatively fast, especially from the 2nd to the 3rd month, with a weight gain rate reaching 406.27 g. Growth rate from IPB-D3 G4 chickens experiences a slowdown at the age of 2 months and falls below their optimal growth rate. Another aspect to report is the mortality rateof IPB-D3 G4 chickens, which is quite high at the DOC and female age due to environmental conditions and disease attacks. However, the mortality rate decreases with the increasing age of the chickens, and no deaths are found in 12 weeks old male chickens. The heritability value of body weight in IPB-D3 G4 chickens is high at the age of 4 weeks and can be used as a basis for selection based on body weight.

Realizing mechanical property improvement and coloration in Si3N4 ceramics by rare earth-doping

Si3N4 ceramics, due to their exceptional comprehensive performance, have potential applications in electronic products under the 5th generation mobile communication technology. However, their development is hindered by a lack of color variety and inherent brittleness. To address these issues, we propose the use of an appropriate colorant-sintering additive to enhance densification, promote the growth of β grains and facilitate the formation of a chromogenic phase. This approach aims to simultaneously adjust the color and mechanical properties of Si3N4 ceramics. STEM-EDS results indicate the presence of Re-enriched hollow structures within the Si3N4 matrix. These hollow structures may serve as one of the color centers of silicon nitride. The combined effects of the stress field surrounding the hollow structure, elongated β-Si3N4 grains and intensive dislocations result in strong mechanical properties of Si3N4 ceramics, in which the flexural strength is 852.7 ± 55.8 MPa and the fracture toughness is 11.7 ± 1.0 MPa m1/2.

Effect of molybdenum on isothermal oxidation behavior of 4th generation nickel-based single crystal superalloys

The isothermal oxidation behavior of fourth-generation nickel-based single crystal superalloys with 2-4 wt% Mo content were studied at 900 °C and 1100 °C.At 1100 °C, the oxide layer can be divided into three layers, the outermost layer is Ni–Co oxide, the intermediate layer is spinel and heavy metal element oxide, and the innermost layer is Al2O3 layer. With the increase of Mo content, the oxide layer accumulates more growth stress, resulting in more severe spallation. At 900 °C, the oxide layer can be divided into three layers, the outermost layer is Ni–Co oxide, the intermediate layer is Cr–Al oxide, and the innermost layer has obvious selective oxidation of Al element. The volatilization of oxide promotes the diffusion of O into the matrix, thus promoting the oxidation of heavy metal elements in the matrix. With the increase of Mo content, this phenomenon is more obvious. The results show that the increase of Mo content of alloy promotes the oxidation of Mo, W and Re elements, which let the volatilization of the oxide and the spalling of the oxide layer more severe, thus decreasing the oxidation resistance of the alloy.

Study on the mechanism of γ' phase rafting in a 4th generation nickel-based single crystal superalloy during thermal exposure at high temperatures

Non-directional rafting of γ' phases in high generation nickel-based single crystal superalloys happens when the alloys are thermally exposed at high temperatures, but so far the reason remains unclear. The microstructures and internal stress fields of a 4th generation single crystal superalloy during thermal exposure at 1100 ℃ and 950 ℃ are investigated using transmission electron microscopy and finite element analysis. It is found that the non-directional rafting of γ' phases occurs after 1100 ℃/200 h and 950 ℃/500 h thermal exposures. The γ' phases are mainly rodlike shape in the space after thermal exposures and a small quantity of γ' rafts exist as well. The elastic strain energy gradient in γ matrix caused by the inhomogeneity of matrix channel width in the alloy is most likely the driving force for the non-directional rafting. Additionally, dislocations are observed only after the thermal exposure process at 1100 ℃. The effect of dislocations on non-directional rafting during thermal exposure is discussed in detail in the paper.

AN ORGANISED AND FOCUSED REVIEW OF A MICROSTRIP PATCH ANTENNA FOR ITS DEPLOYMENT IN BIOMEDICAL APPLICATIONS

Reviewing microstrip antennas for diverse applications is the goal of this paper. The designing of microstrip patch antennae is a new study subject established for utilise in 5th generation communications applications. An antenna is a group of interconnected devices that work together to transmit and receive radio waves as a single antenna. Antennas come in many sizes and shapes. An antenna design that is low profile, lightweight, and results-oriented is the microstrip patch. In the future, microstrip patch antenna may be used for various 6G communication systems applications. Furthermore, 6G communication applications can be developed for additional devices, such as autonomous cars, machine learning, artificial neural network algorithms, radar, internet of things (IoT), biomedical, and vehicle-to-vehicle (V2V) communication. In the past, 4G wireless applications employed the multiple input, numerous output (MIMO) pattern as a standard geometry. This study covers several types of antennas, their geometric structures, different methods for analyzing their features, and their dimensions. The component of the substrate substances, loss tangent, the thickness, return loss, bandwidth, voltage-standing-wave ratio (VSWR), gain, and orientation from the earlier publications will also be covered. Keywords – microstrip, patch, antenna, IoT, biomedical, V2V, MIMO, VSWR

Acute cardiovascular effects of 4th generation electronic cigarettes and combusted cigarettes: implications for harm reduction.

The health consequences associated with using electronic cigarettes (ECs) are of great public interest because of their potential role in smoking cessation. In 110 participants, including 41 nonusers, 34 people who exclusively use ECs (EC group), and 35 people who smoke tobacco cigarettes (TCs) including 12 dual users (collectively called the TC-D group), the heart rate (HR), blood pressure (BP), and heart rate variability (HRV) were compared at baseline. People in the EC or the TC-D groups were also compared after using a 4th generation EC with or without nicotine, a TC with or without nicotine (TC-D group only), and a straw-control. Baseline HR, BP, and HRV parameters were not different among the EC, the TC-D, and nonuser groups. In people who exclusively use ECs, acute nicotine-EC use increased HR and BP, and produced changes in HRV patterns suggestive of increased cardiac sympathetic influence. In people in the TC-D group, BP increased similarly after acutely smoking a nicotine-TC or a nicotine-EC. However, the increase in HR was significantly greater after smoking a TC compared with the nicotine-EC despite similar acute increases in plasma nicotine. Overall, all exposures containing nicotine significantly increased HR and BP in both cohorts when compared with non-nicotine exposures. Since acute EC use 1) produces an abnormal HRV pattern associated with increased cardiac sympathetic tone in people who chronically use ECs, and 2) similar hemodynamic increases compared with acute TC smoking in people who chronically smoke TCs including dual users, the role of ECs as part of a harm reduction strategy is questioned.NEW & NOTEWORTHY We found that nicotine, not the non-nicotine constituents in tobacco cigarette (TC) or electronic cigarette (EC) emissions, may be the instigator of the acute, potentially adverse, changes in hemodynamics and heart rate variability (HRV) that were recorded several minutes after tobacco product use. Furthermore, acute EC use produced an abnormal HRV pattern associated with increased cardiac risk in people who chronically smoke ECs and produced similar hemodynamic increases compared with acute TC use in people who chronically smoke TCs, including people who are dual users.

Interrogating the standardisation of surveillance in 5G amid US–China competition

ABSTRACT In this article we show that the 5G competition between the United States and Western Europe versus China is not reflected in the standardisation of lawful interception (LI) technologies in the world’s leading telecommunications standardisation body, the 3rd Generation Partnership Project (3GPP). Guided by the concept of infrastructure as a site and tool of political contestation, we develop a new approach to the study of Internet governance and standard-setting processes that leverages web scraping and computer-assisted document set discovery software tools. We bring these methods into conversation with theoretical approaches from material media studies, science and technology studies, and international relations. The 3GPP is the main telecommunications standardisation body and the only consortium developing standards to fulfil the ITU’s criteria for the 5th generation of telecommunications technology (5G). The 3GPP therefore is a strategic venue to observe and interpret the politics of standardisation processes. As such, the LI-related work conducted in 3GPP exemplifies public and private actors’ capacity to influence global surveillance standards and export their surveillance technologies. While European and United States governments engage in the standardisation of surveillance technologies, the Chinese government does not do this in the 3GPP. This fuels distrust in 5G technologies. We argue that further integration of China in standardisation could function as a trust-building measure.

Assessing the Vulnerabilities of Bioterrorism: Challenges and Prospects

This study investigates the vulnerabilities and challenges exacerbated by bioterrorism empirically. Terrorists, organizations, and individuals deliberately use biological weapons to kill humans, animals, and plants. According to experts, the threats are multifaceted and have intensified in the modern world, such as 5th generation warfare and health crises. The study is exploratory, employing a qualitative approach to explore the literature, policy documents, and reports released by international and national organizations. In addition, the study integrates the most recent papers published during COVID-19 to investigate the difficulties and potential of bioterrorism. The findings provide a thorough understanding of the expected dangers of bioterrorism, such as threats of theft and attack, threats to utilize biological weapons, which are cost-effective, by terrorist groups, anarchic proliferation across the globe, biological agent manipulation, and so on. During the pandemic COVID-19, several countries, including Pakistan, experienced these risks. Given all of the looming concerns stated above, precautionary actions should be implemented to avoid these threats in the event of a new international bioterror strike in the future.

Cyber Physical System For Autometed Weather Station And Agriculture Node In Smart Farming

The Cyber-Physical System is a key system in the implementation of the 4th generation industrial revolution. This system combines automation systems, electronics, internet networks and machine learning. The implementation of physical cyber systems in agriculture is one of the long-awaited applications because it is the backbone of the implementation of sustainable agribusiness systems. With this system precision agriculture and pervasive computing in agriculture can help stakeholders in agribusiness to enjoy various benefits optimally. One example of its application is a smart farming system that is conditioned to provide measurable and maximum yields without having to sacrifice soil nutrients because it is well monitored according to weather conditions. In previous studies, several LoRa communication-based systems for monitoring local weather in a place and measurements of soil nutrient conditions have been carried out and displayed through the internet network. Monitoring and control systems on other agricultural models based on hydroponics and aquaponics have also been carried out for some types of crops. The results provide greater potential for wider application and in actual conditions in the agricultural industry. In this study, the integration of physical cyber systems with lora communication-based agricultural monitoring and control nodes will be carried out more broadly by considering the local conditions under which the system is tested. In this case, the research will be carried out at the Research and Recreation Park, Telkom University and agrifarming industry partners as a model of actual application. The research findings show that the application of advanced sensor technology has improved the accuracy of weather measurements by up to 95%. Quantitative data collected from the new weather station showed a significant improvement in weather monitoring. Qualitatively, positive responses from stakeholders such as disaster management authorities and farmers were also noted. In conclusion, the development of this modern weather station supports the community's need for reliable weather information and is a step forward in addressing the challenges of climate change.

High Prevalence of Multidrug-Resistant Bacteria in the Trachea of Intensive Care Units Admitted Patients: Evidence from a Bangladeshi Hospital.

Recent research has shown that antibiotic-resistant microorganisms are becoming more prevalent in intensive care units (ICUs) at an exponential rate. Patients in the ICU can get infected by pathogens due to invasive operation procedures and critical health conditions. This study primarily emphasized tracheal samples from ICU patients due to their reliance on ventilators, increasing their susceptibility to Ventilator-Associated Pneumonia (VAP). Moreover, the rise of multidrug-resistant (MDR) pathogens makes treatment strategies more challenging for these patients. In this study, we tested 200 tracheal specimens to determine the prevalence of microorganisms and analyzed the antibiotic susceptibility of these isolates against regular antibiotics, including 4th generation drugs. Among the 273 isolates, 81% were gram-negative bacteria, 10% were gram-positive bacteria, and 9% were fungi. The most prevalent gram-negative bacteria were Acinetobacter spp. (34%), Klebsiella spp. (22%), Pseudomonas spp. (14%), and Escherichia coli (9.2%). The most prevalent gram-positive bacteria were Staphylococcus aureus (5.9%), and the fungi were Candida spp. (7.3%). Among the most prevalent bacteria, except Staphylococcus aureus isolates, around 90% were resistant to multiple drugs, whereas 60% of Acinetobacter spp. and Pseudomonas spp. were extensively drug resistant. Sensitivity analysis against the gram-negative and gram-positive drug panel using a one-way ANOVA test followed by Tukey's post hoc test showed that in the in vitro assay, colistin was the most effective antibiotic against all gram-negative bacteria. In contrast, linezolid, vancomycin, and fusidic acid were most effective against all gram-positive bacteria. Regular monitoring of nosocomial infections and safe management of highly resistant bacteria can help prevent future pandemics.

Gut microbiota variation across generations regarding the diet and life stage in Harmonia axyridis (Coleoptera: Coccinellidae).

We attempt to determine the effect of the dietary switch from a native to non-native prey on the gut microbiota in the predaceous ladybird Harmonia axyridis larvae and adults and examine how the dietary effect may vary across generations. We fed H. axyridis with different diets, native aphid Megoura japonica (Matsumura) versus non-native mealybug Phenacoccus solenopsis (Tinsley), for 5 generations and sequenced microbes in the gut of the 3rd instar larvae and adults of the 1st, 3rd, and 5th generations. In addition, we identified microbes in M. japonica and P. solenopsis. The 2 prey species differed in microbial community as measured by abundances of prevalent microbial genera and diversity. In H. axyridis, abundances of some prevalent microbial genera differed between the 2 diets in the 1st and 3rd generations, but the difference disappeared in the 5th generation; this tendency is more obvious in adults than in larvae. Overall, gut microbial assemblages became gradually cohesive over generations. Microbial diversity differed between diets in the 1st and 3rd generations but became similar in the 5th generation. Major prevalent gut microbial genera are predicted to be associated with metabolic functions of H. axyridis and associated genera are more abundant for consuming the mealybug than the aphid. Our findings from this study suggest that the gut microbiota in H. axyridis is flexible in response to the dietary switch, but tends toward homogeneity in microbial composition over generations.

Camptothecin bioprocessing from Aspergillus terreus, an endophyte of Catharanthus roseus: antiproliferative activity, topoisomerase inhibition and cell cycle analysis

Attenuation of camptothecin (CPT) productivity by fungi with preservation and subculturing is the challenge that halts fungi to be an industrial platform of CPT production. Thus, screening for novel endophytic fungal isolates with metabolic stability for CPT production was the objective. Catharanthus roseus is one of the medicinal plants with diverse bioactive metabolites that could have a plethora of novel endophytes with unique metabolites. Among the endophytes of C. roseus, Aspergillus terreus EFBL-NV OR131583.1 had the most CPT producing potency (90.2 μg/l), the chemical identity of the putative CPT was verified by HPLC, FT-IR, NMR and LC–MS/MS. The putative A. terreus CPT had the same molecular mass (349 m/z), and molecular fragmentation patterns of the authentic one, as revealed from the MS/MS analyses. The purified CPT had a strong activity against MCF7 (5.27 μM) and UO-31 (2.2 μM), with a potential inhibition to Topo II (IC50 value 0.52 nM) than Topo 1 (IC50 value 6.9 nM). The CPT displayed a high wound healing activity to UO-31 cells, stopping their metastasis, matrix formation and cell immigration. The purified CPT had a potential inducing activity to the cellular apoptosis of UO-31 by ~ 17 folds, as well as, arresting their cellular division at the S-phase, compared to the control cells. Upon Plackett–Burman design, the yield of CPT by A. terreus was increased by ~ 2.6 folds, compared to control. The yield of CPT by A. terreus was sequentially suppressed with the fungal storage and subculturing, losing ~ 50% of their CPT productivity by 3rd month and 5th generation. However, the productivity of the attenuated A. terreus culture was completely restored by adding 1% surface sterilized leaves of C. roseus, and the CPT yield was increased over-the-first culture by ~ 3.2 folds (315.2 μg/l). The restoring of CPT productivity of A. terreus in response to indigenous microbiome of C. roseus, ensures the A. terreus-microbiome interactions, releasing a chemical signal that triggers the CPT productivity of A. terreus. This is the first reports exploring the potency of A. terreus, endophyte of C. roseus” to be a platform for industrial production of CPT, with an affordable sustainability with addition of C. roseus microbiome.

Federated learning for green and sustainable 6G IIoT applications

The 6th generation mobile network (6G) is expected to be launched in the early 2030s. The architecture of 6G will be the convergence of space, air, ground, and undersea networks. The power and intelligence of 6G based on advanced AI techniques will realize the concept of the Industrial Internet of Things (IIoT). In this study, we conduct a comprehensive survey of 6G IIoT applications based on Federated Learning (FL), starting from introducing recent advances in FL and IIoT systems to discussing how to integrate them. In particular, we highlight the potential of FL for supporting a range of IIoT systems such as smart medical, intelligent transportation, smart cities, unmanned aerial vehicles, and smart industry. The important discussions to drive FL into IIoT applications are emphasized. Finally, we present challenges and open issues for future research to realize green and suitable FL-based IIoT applications. Index TermsFederated Learning, Artificial Intelligence, Industrial Internet of Things, Smart IoT Applications

One‐Bit Distributed Sparse Spectrum Sensing Based on the DQA‐ZA‐LMS and DQA‐RZA‐LMS Algorithms Over Adaptive Networks

In this paper, we proposed the distributed quantization and sparsity aware zero attracting least mean square (DQA‐ZA‐LMS) and its reweighted version (DQA‐RZA‐LMS) algorithms that can perform sparse spectrum sensing with the lowest power possible. The usage of the quantization aware diffusion adaptive networks has recently been proposed and they can be used in many possible mobile communicative applications. The sparsity aware feature of the proposed algorithm can help the network to track and estimate sparse random vectors that are shown to be the case with the spectrum of the new generation wireless communication systems such as 4G, 5G, 6G, and beyond. The spectrum sensing is considered in this paper to be performed by small cell eNode Bs (SC‐eNBs) for the 4th generation long term evolution (LTE) and the next generation eNB (ng‐eNB) networks for the 5th and 6th generation mobile communication systems that are scattered in an area collecting distributed quantized data from the environment and working collaboratively to estimate the sparse spectrum vectors. Our findings show that in comparison with the nonquantized version of the distributed ZA‐LMS (DZA‐LMS) and distributed regularized ZA‐LMS (DRZA‐LMS) algorithms, our proposed schemes perform considerably well using the quantized data and also reduce power consumption.