Injection Of 3H-TdR Research Articles

Kinetics of proliferation and migration of epithelial cells in the guinea pig colon

Autoradiography combined with tritiated thymidine was used to find some aspects of proliferation and migration kinetics of epithelial cells in the colonic crypts of guinea pig. The results led to the following conclusions: 1. 1. In the colonic crypt of guinea pig there exist two centres of intensive proliferation: the main one near the bottom of the crypt and the additional one near the top. 2. 2. The velocity of cell migration was estimated: ( a) by computing the first moments of labelled cells distribution at various time intervals after 3HTdR injection; ( b) by accumulation of proliferation rates. The results obtained with both methods were found to fit each other well. The cell velocity rose towards the mouth of the crypt where it was 0.7–0.8 cell-width/h.

Observations on the Paneth cell in human ileum

An autoradiographic study of the Paneth cell in human ileum is presented. The low labeling index, low mitotic frequency, and retention of label over a long testing period (18 and 32 days after 3HTdR injection) seen in these cells confirm the slow replacement of this cell type. The observed minimum duration of the G2 (premitotic) phase was 3 hr. Observations on the migration, secretion, and death of the Paneth cell are also reported, as well as estimates of the generation and replacement time of this cell.

Comparative effectiveness of intraperitoneal and intramuscular 3H-TDR injection routes in mice

Comparative effectiveness of intraperitoneal and intramuscular 3H-TDR injection routes in mice

Brief Note: Tritiated Thymidine as a Cytocidal Agent in Human Leukemia

Abstract 1. Two patients with acute leukemia had considerable decreases in leukemic cells in the peripheral blood as well as reduction in size of spleen and leukemic masses after 10 injections of 3H-TDR given over a 5-day period. Each injection was 0.25 µc./Gm. body weight. 2. The pertinent aspects of cytotoxic effects of 3H-TDR are reviewed. 3. The radiation doses delivered to the nucleus are estimated from autoradiographic data. 4. Evidence is presented for the observed effects being due to 3H-TDR.