1D Spectroscopy Research Articles

Synergistic photocatalysis for naphthalene (NAP) removal in seawater by S-scheme heterojunction Bi2S3/CeVO4: Mechanistic investigation and degradation pathways

The construction of S-scheme heterostructure is a prospective strategy to remove polycyclic aromatic hydrocarbons (PAHs) from simulated seawater. In this study, a novel Bi2S3/CeVO4 S-scheme heterojunction photocatalyst was prepared by solvent thermal method. The successful construction of S-scheme heterostructure is beneficial to the separation and migration of photogenerated carriers, thus achieving excellent redox capacity and efficient production of free radicals, which is conducive to promoting the photocatalyst to transform pollutants into H2O, CO2. In pure water, the optimized Bi2S3/CeVO4 heterojunction (Bi/Ce-30) achieved a high degradation efficiency (97.39 %) for naphthalene (NAP). In simulated seawater, the degradation efficiency of NAP still reaches 87.20 % (k = 0.01653 min−1), which is 7.58 folds higher than pure CeVO4. Furthermore, the effect (such as solution pH, pollutant concentration, etc.) on the photocatalytic performance of Bi2S3/CeVO4 heterojunction was investigated. The possible photocatalytic degradation processes of NAP were qualitatively analyzed using 3D fluorescence spectroscopy and HPLC-MS to reveal the major degradation pathways. In addition, toxicity assessment software was used to demonstrate the gradual decrease in toxicity of the intermediate products. Furthermore, we elucidated the possible photocatalytic mechanism of Bi2S3/CeVO4 S-scheme heterojunction for the degradation of NAP. This work provides new insights into the realization of S-scheme heterojunction for removing polycyclic aromatic hydrocarbons from natural seawater.

Probing the binding interactions between perfluoroalkyl carboxylic acids and adenosine A2A receptors by spectroscopic techniques, molecular simulations and quantum chemistry

The binding interactions of perfluorobutyric acid (PFBA), heptafluorobutyric anhydride (HFBA), and ethyl heptafluorobutyrate (NSC3936) with the adenosine A2A receptor (A2AR) were investigated using multiple-spectral (fluorescence, ultraviolet–visible (UV–Vis) absorption, three-dimensional (3D) fluorescence, and Fourier Transform infrared (FT-IR) spectroscopy) and computational simulation methods. Steady-state fluorescence and UV–visible spectroscopy indicated that PFBA, HFBA, and NSC3936 caused an intrinsic fluorescence quenching of A2AR by static quenching and non-radiative energy transfer. The results of 3D fluorescence and FT-IR spectroscopy showed that the presence of PFBA, HFBA, and NSC3936 induced changes in the secondary structure of A2AR. Combined with thermodynamic analysis, molecular docking and quantum chemistry investigations, our results suggest that the association of PFBA, HFBA, and NSC3936 with A2AR was driven by hydrophobic forces and the formation of hydrogen bonds (H-bonds). The binding constants for the three PFCAs studied range from 8.27 × 106 L·mol−1 to 1.80 × 107 L·mol−1 at 298 K. Molecular dynamics (MD) simulations indicated that A2AR formed stable complexes with PFBA, HFBA, and NSC3936, and validated the existence of electrostatic interactions in the HFBA-A2AR complex. Furthermore, the results from the MD simulations were consistent with the thermodynamic analysis results.

MUSE crowded field 3D spectroscopy in NGC 300

Aims. We perform a deep survey of planetary nebulae (PNe) in the spiral galaxy NGC 300 to construct its planetary nebula luminosity function (PNLF). We aim to derive the distance using the PNLF and to probe the characteristics of the most luminous PNe. Methods. We analysed 44 fields observed with MUSE at the VLT, covering a total area of ∼11 kpc2. We find [O III]λ5007 sources using the differential emission line filter (DELF) technique. We identified PNe through spectral classification with the aid of the BPT diagram. The PNLF distance was derived using the maximum likelihood estimation technique. For the more luminous PNe, we also measured their extinction using the Balmer decrement. We estimated the luminosity and effective temperature of the central stars of the luminous PNe based on estimates of the excitation class and the assumption of optically thick nebulae. Results. We identify 107 PNe and derive a most-likely distance modulus $ (m-M)_0 = 26.48^{+0.11}_{-0.26} $ ($ d = 1.98^{+0.10}_{-0.23} $ Mpc). We find that the PNe at the PNLF cutoff exhibit relatively low extinction, with some high-extinction cases caused by local dust lanes. We present the lower limit luminosities and effective temperatures of the central stars for some of the brighter PNe. We also identify a few Type I PNe that come from a young population with progenitor masses > 2.5 M⊙ but do not populate the PNLF cutoff. Conclusions. The spatial resolution and spectral information of MUSE allow precise PN classification and photometry. These capabilities also enable us to resolve possible contamination by diffuse gas and dust, improving the accuracy of the PNLF distance to NGC 300.

C-Terminal p53 Palindromic Tetrapeptide Restores Full Apoptotic Function to Mutant p53 Cancer Cells In Vitro and In Vivo.

We previously demonstrated that a synthetic monomer peptide derived from the C-terminus of p53 (aa 361−382) induced preferential apoptosis in mutant p53 malignant cells, but not normal cells. The major problem with the peptide was its short half-life (half-life < 10 min.) due to a random coil topology found in 3D proton NMR spectroscopy studies. To induce secondary/tertiary structures to produce more stability, we developed a peptide modelled after the tetrameric structure of p53 essential for activation of target genes. Starting with the above monomer peptide (aa 361−382), we added the nuclear localization sequence of p53 (aa 353−360) and the end of the C-terminal sequence (aa 383−393), resulting in a monomer spanning aa 353−393. Four monomers were linked by glycine to maximize flexibility and in a palindromic order that mimics p53 tetramer formation with four orthogonal alpha helices, which is required for p53 transactivation of target genes. This is now known as the 4 repeat-palindromic-p53 peptide or (4R-Pal-p53p). We explored two methods for testing the activity of the palindromic tetrapeptide: (1) exogenous peptide with a truncated antennapedia carrier (Ant) and (2) a doxycycline (Dox) inducer for endogenous expression. The exogenous peptide, 4R-Pal-p53p-Ant, contained a His tag at the N-terminal and a truncated 17aa Ant at the C-terminal. Exposure of human breast cancer MB-468 cells and human skin squamous cell cancer cells (both with mutant p53, 273 Arg->His) with purified peptide at 7 µM and 15 µM produced 52% and 75%, cell death, respectively. Comparatively, the monomeric p53 C-terminal peptide-Ant (aa 361−382, termed p53p-Ant), at 15 µM and 30 µM induced 15% and 24% cell death, respectively. Compared to the p53p-Ant, the exogenous 4R-pal-p53p-Ant was over five-fold more potent for inducing apoptosis at an equimolar concentration (15 µM). Endogenous 4R-Pal-p53p expression (without Ant), induced by Dox, resulted in 43% cell death in an engineered MB468 breast cancer stable cell line, while endogenous p53 C-terminal monomeric peptide expression produced no cell death due to rapid peptide degradation. The mechanism of apoptosis from 4R-Pal-p53p involved the extrinsic and intrinsic pathways (FAS, caspase-8, Bax, PUMA) for apoptosis, as well as increasing reactive oxygen species (ROS). All three death pathways were induced from transcriptional/translational activation of pro-apoptotic genes. Additionally, mRNA of p53 target genes (Bax and Fas) increased 14-fold and 18-fold, respectively, implying that the 4R-Pal-p53p restored full apoptotic potential to mutant p53. Monomeric p53p only increased Fas expression without a transcriptional or translational increase in Fas, and other genes and human marrow stem cell studies revealed no toxicity to normal stem cells for granulocytes, erythrocytes, monocytes, and macrophages (CFU-GEMM). Additionally, the peptide specifically targeted pre-malignant and malignant cells with mutant p53 and was not toxic to normal cells with basal levels of WT p53.

Quadrangulosides A–F: new pregnane glycosides from Caralluma quadrangula

The phytochemical investigation of Caralluma quadrangula aerial parts yielded six new pregnane glycosides, quadrangulosides A-F (1-6), in addition to nine known pregnane glycosides and three known flavone glycosides. Structures of isolated phyto-constituents were elucidated via spectroscopic 1D-, 2D-NMR and spectrometric ESI-MS spectra.

Ferrous-Iron-Activated Sulfite-Accelerated Short-Chain Fatty Acid Production from Waste-Activated Sludge Fermentation: Process Assessment and Underlying Mechanism

To break the bottlenecks of slow hydrolysis and low acid production efficiency of waste-activated sludge (WAS) in the traditional anaerobic fermentation process, this study investigated the employment of ferrous-iron (Fe(II))-activated sulfite to produce hydroxyl, sulfate, and other highly oxidizing radicals on WAS floc cracking and short-chain fatty acid (SCFAs) production during anaerobic fermentation. The effect of the dosage ratio of Fe(II)/S(IV) was also studied. Results showed that the combined pretreatment of Fe(II)-activated sulfite significantly promoted the exfoliation of extracellular polymers and the subsequent SCFAs production. The highest concentration of SCFAs reached 7326.5 mg COD/L under the optimal dosage of 1:2 for Fe(II)/S(IV), which was 1.1~2.1 times higher than that of other research groups. Meanwhile, the analysis by 3D fluorescence spectroscopy and EPR (electron paramagnetic resonance) showed that Fe(II)-activated sulfite had a synergistic effect on the rupture of sludge cells and the stripping of extracellular polymers, with SO4− and OH as the key radicals generated and being much stronger in the 1:1 and 1:2 groups. High-throughput sequencing showed that the Fe(II)-activated sulfite system significantly changed the functional microbial diversity. The anaerobic fermentation bacteria and sulfate-reducing bacteria were significantly enriched. The underlying mechanism of Fe(II)-activated sulfite oxidation and molecular ecological network of key microbiomes were unveiled.

Development of 2D and 3D front face fluorescence spectroscopy for monitoring ultrasound treatment in the removal of pesticides residues from fresh lettuces at the laboratory and pilot scales

Pesticide residues in vegetables are potentially toxic components to humans and can cause serious health problems. To remove pesticide residues from fresh agricultural products and improve consumer food safety, various pesticide removal methods have been investigated over the past decades. In this study, the effectiveness of laboratory and pilot scale ultrasonic cleaning on the removal of boscalid and pyraclostrobin residues from lettuce was examined. 2D fluorescence spectroscopy, 3D fluorescence spectroscopy represented by excitation-emission matrix (EEM), and parallel factor analysis (PARAFAC) were used to characterize and discriminate the fluorescence signatures of these pesticides in the cleaning water to determine the effectiveness of the ultrasonic cleaning method as a function of the level of pesticide removal. The 2D fluorescence results showed that the rate of removal of boscalid by ultrasonics at the laboratory scale increased with the cleaning time. The ultrasonic treatment showed a higher cleaning efficiency compared to only soaking in distilled water for 10 min. The same trends were observed at the pilot scale. The EEM also showed differences in the concentration of pesticides removed by ultrasonication between the different parts of the lettuce, the concentration was higher in the upper part than the lower part. This study showed that ultrasonication is an effective technique for the removal of pesticide residues on lettuce, and it also showed the significant potential of fluorescence spectroscopy coupled with PARAFAC for the discrimination and characterization of pesticides.

Performance of electrochemical treatment of refractory organic matter in printing and dyeing reverse osmosis concentrate

The printing and dyeing wastewater treatment process produces a reverse osmosis concentrate (ROC) that contains high organic matter content and requires proper treatment before discharge. Electrochemical oxidation was applied in this study to treat organic pollutants in the ROC. In addition, the concentration variation of phthalate esters (PAEs) in ROC was investigated. The results demonstrated that the electrochemical oxidation process could effectively reduce the organic content in ROC; the total organic carbon (TOC) removal efficiency reached 83.38% at pH 2.4 and a current density of 40 mA/cm2. Increasing the current density can markedly enhance the oxidation of the bulk organic matter (i.e., TOC, chemical oxygen demand (COD), UV254, and fluorescence). The effect of pH on the decomposition of organic materials was slightly less as than that of current density. The molecular weight (MW) distribution in the ROC indicated that the percentage of components larger than 5 kDa was approximately 10%, but decreased to 2.16% with persistent electrolysis. Further, 3D fluorescence spectroscopy suggests that the organic matter in ROC was mainly aromatic proteins (APⅡ) and soluble microbial products (SMP), which account for 25.78% and 51.39% of the total fluorescence, respectively. Notably, acidic conditions are more favorable for the breakdown of fluorescence, which is attributed to the higher oxidative capacity of·OH. The decomposition of PAEs was enhanced by lowering the pH, and degradation efficiency increased by 36.45% when the pH was lowered from 9.8 to 2.4. Qualitative analysis by gas chromatography-mass spectrometry (GC-MS) showed that the abundance of organic matter in the treated ROC was significantly decreased.

Fluorescence-Detected Two-Quantum Photon Echoes via Cogwheel Phase Cycling.

Two-dimensional (2D) electronic spectroscopy can separate homogeneous and inhomogeneous broadening. While established methods usually probe a photon-echo signal, i.e., a third-order response, to access the homogeneous line width of singly excited states, the homogeneous line width of doubly excited states remained spectroscopically inaccessible. Here we demonstrate the acquisition of two-quantum (2Q) photon echoes using fluorescence-detected 2D spectroscopy. In these eighth-order signals, 2Q coherences are rephased with themselves, leading to line-narrowed 2Q-2Q 2D spectra. By using cogwheel phase cycling, adapted from nuclear magnetic resonance spectroscopy, we isolate the 2Q-2Q 2D spectra of a squaraine dimer and a squaraine polymer and verify the same selectivity of cogwheel phase cycling compared to traditional "nested" phase cycling. The observed difference, between the two systems, in the homogeneous line width of the biexciton can be rationalized as a signature of the interplay of exciton-exciton annihilation, exciton diffusion, and exciton delocalization.

MUSE crowded field 3D spectroscopy in NGC 300

Context. There are known differences between the physical properties of H II and diffuse ionized gas (DIG). However, most of the studied regions in the literature are relatively bright, with log10 L(Hα)[erg s−1] ≳ 37. Aims. We compiled an extremely faint sample of 390 H II regions with a median Hα luminosity of 34.7 in the flocculent spiral galaxy NGC 300, derived their physical properties in terms of metallicity, density, extinction, and kinematics, and performed a comparative analysis of the properties of the DIG. Methods. We used MUSE data of nine fields in NGC 300, covering a galactocentric distance of zero to ~450 arcsec (~4 projected kpc), including spiral arm and inter-arm regions. We binned the data in dendrogram leaves and extracted all strong nebular emission lines. We identified H II and DIG regions and compared their electron densities, metallicity, extinction, and kinematic properties. We also tested the effectiveness of unsupervised machine-learning algorithms in distinguishing between the H II and DIG regions. Results. The gas density in the H II and DIG regions is close to the low-density limit in all fields. The average velocity dispersion in the DIG is higher than in the H II regions, which can be explained by the DIG being 1.8 kK hotter than H II gas. The DIG manifests a lower ionization parameter than H II gas, and the DIG fractions vary between 15–77%, with strong evidence of a contribution by hot low-mass evolved stars and shocks to the DIG ionization. Most of the DIG is consistent with no extinction and an oxygen metallicity that is indistinguishable from that of the H II gas. We observe a flat metallicity profile in the central region of NGC 300, without a sign of a gradient. Conclusions. The differences between extremely faint H II and DIG regions follow the same trends and correlations as their much brighter cousins. Both types of objects are so heterogeneous, however, that the differences within each class are larger than the differences between the two classes.

Theory of rotationally resolved two-dimensional infrared spectroscopy including polarization dependence and rotational coherence dynamics

Two-dimensional infrared (2DIR) spectroscopy is widely used to study molecular dynamics but it is typically restricted to solid and liquid phase samples and modest spectral resolution. Only recently, its potential to study gas-phase dynamics is beginning to be realized. Moreover, the recently proposed technique of cavity-enhanced 2D spectroscopy using frequency combs and developments in multi-comb spectroscopy are expected to dramatically advance capabilities for acquisition of rotationally-resolved 2DIR spectra. This demonstrates the need for rigorous and quantitative treatment of rotationally-resolved, polarization-dependent third-order response of gas-phase samples. In this article, we provide a rigorous and quantitative description of rotationally-resolved 2DIR spectroscopy using density-matrix, time-dependent perturbation theory and angular momentum algebra techniques. We describe the band and branch structure of 2D spectra, decompose the molecular response into polarization-dependence classes, use this decomposition to derive and explain special polarization conditions and relate the liquid-phase polarization conditions to gas-phase ones. Furthermore, we discuss the rotational coherence dynamics during waiting time.

High-resolution 2D spectroscopy shows molecules in a new light

High-resolution 2D spectroscopy shows molecules in a new light

Neumann's principle based eigenvector approach for deriving non-vanishing tensor elements for nonlinear optics.

Physical properties are commonly represented by tensors, such as optical susceptibilities. The conventional approach of deriving non-vanishing tensor elements of symmetric systems relies on the intuitive consideration of positive/negative signflipping after symmetry operations, which could be tedious and prone to miscalculation. Here, we present a matrix-based approach that gives a physical picture centered on Neumann's principle. The principle states that symmetries in geometric systems are adopted by their physical properties. We mathematically apply the principle to the tensor expressions and show a procedure with clear physical intuition to derive non-vanishing tensor elements based on eigensystems. The validity of the approach is demonstrated by examples of commonly known second and third-order nonlinear susceptibilities of chiral/achiral surfaces, together with complicated scenarios involving symmetries such as D6 and Oh symmetries. We then further applied this method to higher-rank tensors that are useful for 2D and high-order spectroscopy. We also extended our approach to derive nonlinear tensor elements with magnetization, which is critical for measuring spin polarization on surfaces for quantum information technologies. A Mathematica code based on this generalized approach is included that can be applied to any symmetry and higher order nonlinear processes.

2D spectroscopies from condensed phase dynamics: Accessing third-order response properties from equilibrium multi-time correlation functions

The third-order response lies at the heart of simulating and interpreting nonlinear spectroscopies ranging from two-dimensional infrared (2D-IR) to 2D electronic (2D-ES), and 2D sum frequency generation (2D-SFG). The extra time and frequency dimensions in these spectroscopic techniques provide access to rich information on the electronic and vibrational states present, the coupling between them, and the resulting rates at which they exchange energy that are obscured in linear spectroscopy, particularly for condensed phase systems that usually contain many overlapping features. While the exact quantum expression for the third-order response is well established, it is incompatible with the methods that are practical for calculating the atomistic dynamics of large condensed phase systems. These methods, which include both classical mechanics and quantum dynamics methods that retain quantum statistical properties while obeying the symmetries of classical dynamics, such as LSC-IVR, centroid molecular dynamics, and Ring Polymer Molecular Dynamics (RPMD), naturally provide short-time approximations to the multi-time symmetrized Kubo transformed correlation function. Here, we show how the third-order response can be formulated in terms of equilibrium symmetrized Kubo transformed correlation functions. We demonstrate the utility and accuracy of our approach by showing how it can be used to obtain the third-order response of a series of model systems using both classical dynamics and RPMD. In particular, we show that this approach captures features such as anharmonically induced vertical splittings and peak shifts while providing a physically transparent framework for understanding multidimensional spectroscopies.

Enantiodetection of Chiral Molecules via Two-Dimensional Spectroscopy.

Enantiodetection of chiral molecules is important to chemical reaction control and biological function designs. Traditional optical methods of enantiodetection rely on the weak magnetic-dipole or electric-quadrupole interactions, and in turn suffer from the weak signal and low sensitivity. We propose a new optical enantiodetection method to determine the enantiomeric excess via two-dimensional (2D) spectroscopy of the chiral mixture driven by three electromagnetic fields. The quantities of left- and right-handed chiral molecules are reflected by the intensities of different peaks on the 2D spectrum, separated by the chirality-dependent frequency shifts resulting from the relative strong electric-dipole interactions between the chiral molecules and the driving fields. Thus, the enantiomeric excess can be determined via the intensity ratio of the peaks for the two enantiomers.

Mapping Simulated Two-Dimensional Spectra to Molecular Models Using Machine Learning.

Two-dimensional (2D) spectroscopy encodes molecular properties and dynamics into expansive spectral data sets. Translating these data into meaningful chemical insights is challenging because of the many ways chemical properties can influence the spectra. To address the task of extracting chemical information from 2D spectroscopy, we study the capacity of simple feedforward neural networks (NNs) to map simulated 2D electronic spectra to underlying physical Hamiltonians. We examined hundreds of simulated 2D spectra corresponding to monomers and dimers with varied Franck-Condon active vibrations and monomer-monomer electronic couplings. We find the NNs are able to correctly characterize most Hamiltonian parameters in this study with an accuracy above 90%. Our results demonstrate that NNs can aid in interpreting 2D spectra, leading from spectroscopic features to underlying effective Hamiltonians.

Regularized 3D spectroscopy with CubeFit: Method and application to the Galactic Center circumnuclear disk

Context. The Galactic Center black hole and the nuclear star cluster are surrounded by a clumpy ring of gas and dust, the circumnuclear disk (CND), that rotates about them at a standoff distance of ≃1.5 pc. The mass and density of individual clumps in the CND are disputed. Aims. We seek to use H2 to characterize the clump size distribution and to investigate the morphology and dynamics of the interface between the ionized interior layer of the CND and the molecular reservoir lying farther out (corresponding to the inner rim of the CND, illuminated in ultraviolet light by the central star cluster). Methods. We have observed two fields of approximately 20″ × 20″ in the CND at near-infrared wavelengths with the OSIRIS spectro-imager at the Keck Observatory. These two fields, located at the approaching and receding nodes of the CND, best display this interface. Our data cover two H2 lines as well as the Brγ line (tracing H II). We have developed the tool CubeFit, an original method for extracting maps of continuous physical parameters (such as the velocity field and velocity dispersion) from integral-field spectroscopy data, using regularization to largely preserve spatial resolution in regions of low signal-to-noise ratio. Results. This original method enables us to isolate compact, bright features in the interstellar medium of the CND. Several clumps in the southwestern field assume the appearance of filaments, many of which are parallel to one another. We conclude that these clumps cannot be self-gravitating.

Measuring Protein Conformation at Aqueous Interfaces with 2D Infrared Spectroscopy of Emulsions.

Determining the secondary and tertiary structures of proteins at aqueous interfaces is crucial for understanding their function, but measuring these structures selectively at the interface is challenging. Here we demonstrate that two-dimensional infrared (2D-IR) spectroscopy of protein stabilized emulsions offers a new route to measuring interfacial protein structure with high levels of detail. We prepared hexadecane/water oil-in-water emulsions stabilized by model LK peptides that are known to fold into either α-helix or β-sheet conformations at hydrophobic interfaces and measured 2D-IR spectra in a transmission geometry. We saw clear spectral signatures of the peptides folding at the interface, with no detectable residue from remaining bulk peptides. Using 2D spectroscopy gives us access to correlation and dynamics data, which enables structural assignment in cases where linear spectroscopy fails. Using the emulsions allows one to study interfacial spectra with standard transmission geometry spectrometers, bringing the richness of 2D-IR to the interface with no additional optical complexity.

The Distribution of DOM in the Wanggang River Flowing into the East China Sea.

Dissolved organic matter (DOM) is a central component in the biogeochemical cycles of marine and terrestrial carbon pools, and its structural features greatly impact the function and behavior of ecosystems. In this study, the Wanggang River, which is a seagoing river that passes through Yancheng City, was selected as the research object. Three-dimensional (3D) fluorescence spectral data and UV–visible spectral data were used for component identification and source analysis of DOM based on the PARAFAC model. The results showed that the DOM content of the Wanggang River during the dry season was significantly higher than during the wet season; the DOM content increased gradually from the upper to lower reaches; the proportion of terrigenous components was higher during the wet season than during the dry. UV–Vis spectral data a280 and a355 indicated that the relative concentrations of protein-like components in the DOM of the Wanggang River were higher than those of humic-like components, and the ratio of aromatic substances in the DOM of the Wanggang River water was higher during the wet season. The DOM in the Wanggang River was dominated by protein-like components (>60%), and the protein-like components were dominated by tryptophan proteins (>40%). This study showed that the temporal and spatial distributions of DOM in rivers can be accurately determined using 3D fluorescence spectroscopy combined with the PARAFAC model. This provides useful insight into the biogeochemical process of DOM in rivers of coastal areas.

Beating signals in CdSe quantum dots measured by low-temperature 2D spectroscopy.

Advances in ultrafast spectroscopy can provide access to dynamics involving nontrivial quantum correlations and their evolutions. In coherent 2D spectroscopy, the oscillatory time dependence of a signal is a signature of such quantum dynamics. Here, we study such beating signals in electronic coherent 2D spectroscopy of CdSe quantum dots (CdSe QDs) at 77K. The beating signals are analyzed in terms of their positive and negative Fourier components. We conclude that the beatings originate from coherent LO-phonons of CdSe QDs. No evidence for the QD size dependence of the LO-phonon frequency was identified.