131I, commonly used in nuclear medicine, can be incorporated into the human body in a variety of chemical and physical forms. This study describes a sensitive method for the determination of 131I concentration and its application to the estimation of the amount of airborne 131I inhaled by staff workers of a Nuclear Medicine Department. Our method uses passive sampling with charcoal canisters followed by liquid scintillation counting, a very sensitive technique for the determination of low concentrations of airborne 131I. We have studied the effects caused on the measured 131I concentration in air by both, the doses administered to the patients and the distance of sampling canisters to the patient. We have found a direct relationship between the amount of 131I activity administered and the airborne 131I activity concentration. We have estimated the contribution of inhaled 131I to the effective dose received by staff, finding differences between physicians and non-physician personnel. However, the measured exposure was quite low in both cases, thus suggesting that there is no need to introduce additional safety measures to the current ones.