Abstract
Vitamin D enhances production of soluble ST2, inhibiting the action of IL-33
Highlights
In our cohort of patients with severe asthma, the majority receiving very high doses of inhaled or systemic corticosteroids, a blood eosinophil count of greater than 0.45 3 109 cells/L can correctly predict sputum eosinophilia in 9 of 10 cases
As demonstrated both here and in the article from the Severe Asthma Research Program group,[4] this cutoff would include many patients who do not have contemporaneous sputum eosinophilia and potentially would not be in the group of patients who could benefit from treatment
The consequence of this in a trial of a biologic agent is that the magnitude of benefit could be underestimated or even missed, as happened previously when mepolizumab was trialed in unselected patients.[9]
Summary
Ultrahigh-purity 1,25(OH)D3 and 25(OH)D3 (Enzo Life Sciences, Exeter, United Kingdom) were placed in aliquots dissolved in dimethyl sulfoxide (Sigma-Aldrich, Gillingham, United Kingdom). Recombinant proteins were obtained as follows: IL-33, IFN-g, IL-4, human sST2-Fc chimera, and isotype were all from R&D System (Abingdon, United Kingdom)
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call