Abstract
Colorless apoferritin ( M≅450 kDa) crystals were grown in the presence of red colored holoferritin dimers ( M≅900 kDa) within agarose gel by counterdiffusion with Cd 2+ as precipitant. In agreement with our previous quantitative measurements showing preferential trapping of dimers (segregation coefficient K=4), the apoferritin crystals became darkly colored while the gel solution around them was nearly colorless. This segregation coefficient and the absence of convective mixing resulted in a large colorless area surrounding the crystals which allowed us to demonstrate the existence of the impurity depletion zone by direct visual observation.
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