Abstract
More than ever, rapid and precise methods for detection of bacterial and viral pathogens are requested by our crowded society. In this study, we have detected label-free NASBA RNA amplicons of human pathogens using two sensing systems: binary (split) peroxidase-like deoxyribozyme (Dz) and a cascade of RNA-cleaving and peroxidase-like Dz. We accomplished detection of <i>Escherichia coli</i> and <i>Streptococcus pneumoniae</i>, as well as the DNA virus from Herpes group, HSV1. The sensitivity of the system was as low as 10 bacterial or 10 virus infected cells. The method requires regular boiling-water bath, 1.5 h of total time and 30 min hands-on time for split technology and around 3 h total time with 1 h 30 min hands-on time for cascade sensor with obvious benefits and binary sensor usability. The total cost of reagents and disposables is no more than 1 $ per test. This study demonstrates that both methods can be used for selective and costefficient detection of human pathogens with naked eye. The study lays a foundation for point-of-care diagnostics of infectious diseases with instrument-free visual output signal.
Published Version
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