Year-end Sale % OFF 
Abstract
BackgroundKnowledge on the etiology of LRTIs is essential for improvement of the clinical diagnosis and accurate treatment. Molecular detection methods were applied to identify a broad range of bacterial and viral pathogens in a large set of bronchial alveolar lavage (BAL) fluid samples. The patterns of detected pathogens were correlated to the clinical symptoms.MethodsBAL fluid samples and clinical data were collected from 573 hospitalized children between 1 month and 14 years of age with LRTIs, enrolled from January to December 2018. Pathogens were detected using standardized clinical diagnostics, with a sensitive, high-throughput GeXP-based multiplex PCR and with multiplex qPCR. Data were analyzed to describe the correlation between the severity of respiratory tract disease and the pathogens identified.ResultsThe pathogen detection rate with GeXP-based PCR and multiplex qPCR was significantly higher than by clinical routine diagnostics (76.09% VS 36.13%,χ2 = 8.191, P = 0.004). The most frequently detected pathogens in the BAL fluid were human adenovirus (HADV)(21.82%), Mycoplasma pneumoniae (20.24%), human rhinovirus (13.96%), Streptococcus pneumoniae (8.90%) and Haemophilus influenzae (8.90%). In 16.4% of the cases co-detection with two or three different pathogens was found. Viral detection rates declined with age, while atypical pathogen detection rates increased with age. Oxygen supply in the HADV and Influenza H1N1 infected patients was more frequent (49.43%) than in patients infected with other pathogens.ConclusionBroad range detection of viral and bacterial pathogens using molecular methods is a promising and implementable approach to improve clinical diagnosis and accurate treatment of LRTI in children.
Highlights
Knowledge on the etiology of Lower respiratory tract infections (LRTIs) is essential for improvement of the clinical diagnosis and accurate treatment
The etiology is complex as a whole range of different pathogens, mainly viruses and bacteria, in different combinations, can cause LRTI
The collection of bronchial alveolar lavage (BAL) fluid was done within the Department of Respiratory Diseases in Shenzhen Children’s Hospital for pathogen identification using standardized clinical diagnostic assays, as described here below, to facilitate the clinical decision for treatment
Summary
Knowledge on the etiology of LRTIs is essential for improvement of the clinical diagnosis and accurate treatment. Lower respiratory tract infections (LRTI) are the leading cause of morbidity and mortality in children aged < 5 years worldwide [1]. The etiology is complex as a whole range of different pathogens, mainly viruses and bacteria, in different combinations, can cause LRTI. Accurate detection of respiratory tract pathogens is crucial for the clinical diagnosis and for the epidemiology of LRTIs [3]. Bronchoalveolar lavage (BAL) has been considered the gold standard for detection of the causative agents of lower respiratory tract infections. Large studies detecting both viral and bacterial pathogens are needed to better understand the cause-effect relation, the course of disease and the level of severity to improve treatment and accelerate the recovery from LRTI
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
