Abstract
Infection of BHK 21 13 cells with FV 3 inhibited host cell RNA synthesis within 1 hour and DNA synthesis between 2 and 4 hours after infection. Between 3 and 4 hours a new species of DNA was synthesized in the cell cytoplasm and by 5 hours reached a maximal rate equal to 6 times that of DNA synthesis in uninfected cells. This new DNA had a G + C content of 53% (ϱ = 1.712 g/ml). Virus-induced RNA synthesis began within 2 hours after infection and reached a maximal rate at 5–6 hours. This new RNA was found in the cytoplasm at the periphery of areas of DNA synthesis. Infectious virus was detected 6 to 7 hours after infection.
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