Abstract
Bacterial biopesticide formulations needed because the use of bacteria in the suspensions can reduce the ability to control disease in plants. Therefore, a bacterial suspension needs to be mobilized in the formula with a carrier (Carrier) to maintain the viability of bacteria. This research uses the formulation of Bacillus sp. made from rice flour, corn, tapioca and talc with the addition of urea, glucose and CMC. Formulation of Bacillus sp. flour based with the addition of urea, glucose and CMC were tested with Xanthomonas axonopodis pv. glycines and Colletotrichum sp. in vitro. The results showed that Bacillus sp. after formulation it is still able to maintain viabiitas and antagonistic power. Viability of Bacillus sp. the best is in the formulation of rice flour at 14 hsi at 4.94 X 1014 cfu / g while the inhibition zone of Bacillus sp. the best is the corn flour formulation of 13.1 mm and the inhibitory capacity of Bacillus sp. the best is in tapioca flour formulation at 42 hsi at 62.89%
Highlights
Formulasi yang dapat dilakukan yaitu dengan menggunakan bahan dasar media tepung, seperti tepung tapioka, tepung beras dan tepung jagung (Muis dkk.,2015)
The results showed that Bacillus sp. after formulation it is still able to maintain viabiitas and antagonistic power
3. Formulasi tepung tapioka + urea + glukosa + CMC dengan masa simpan 42 hsi menjadi perlakuan terbaik sehingga mendukung daya hambat Bacillus sp
Summary
Peremajaan Bacillus sp. (koleksi dari Dr Suhartiningsih Dwi Nurcahyati S.P, M.Si) dengan cara mengambil 1 ose isolat kemudian digoreskan pada media NA lalu diinkubasi selama 48 jam. (koleksi dari Dr Suhartiningsih Dwi Nurcahyati S.P, M.Si) dengan cara mengambil 1 ose isolat kemudian digoreskan pada media NA lalu diinkubasi selama 48 jam. Glycines (koleksi dari Dr Suhartiningsih Dwi Nurcahyanti S.P, M.Si) dengan cara mengambil 1 ose isolat kemudian digoreskan pada media NA lalu diinkubasi selama 48 jam. (koleksi Hurin Nabila) dengan cara mengambil 1 plong isolat kemudian ditanam pada media YPGA lalu diinkubasi selama 48 jam. Terhadap Xag dapat dilakukan dengan menggunakan metode dual planting yaitu dengan cara menumbuhkan isolat Bacillus sp. Setelah itu menuangkan suspensi Xag sebanyak 0,2 ml dalam agar air 0,6% sebanyak 4 ml lalu diinkubasi selama 24 jam untuk diamati zona hambatan (Saputra dkk., 2015). Dapat dilakukan dengan cara mengambil isolat Bacillus sp. Ditengah dan 4 titik Bacillus dipinggir cawan petri dengan jarak 2 cm dari tepi cawan petri sebagai perlakuan (Muharni dan Hary, 2011). Setelah itu menyiapkan suspensi Bacillus sp. dengan populasi 1013 cfu/ml dan mencampurkan ke dalam masingmasing media tepung
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