Variability in the Qualitative and Quantitative Composition and Content of Phenolic Compounds in the Fruit of Introduced American Cranberry (Vaccinium macrocarpon Aiton).

Ieva Gudžinskaitė,Kristina Zymonė,Jonas Viškelis,Rima Urbštaitė,Mindaugas Liaudanskas,Elicija Stackevičienė,Vaidotas Žvikas,Valdimaras Janulis

doi:10.3390/plants9101379

Abstract

The aim of this study was to determine the composition and content of phenolic compounds in ethanol extracts of eight different cultivars of American cranberry (Vaccinium macrocarpon Aiton) fruit using spectrophotometric and UPLC-ESI-MS/MS analysis and to evaluate the antioxidant activity in vitro of these extracts. The highest total amount of phenolic compounds evaluated via Folin–Ciocalteu spectrophotometry was detected in American cranberry fruit samples of the ‘Bain’ clone, and the highest total amount of flavonoids was found in samples of the ‘Drever’ and ‘Baiwfay’ cultivars. The highest total amount of the individual phenolic compounds (519.53 ± 25.12 mg/g DW) identified and quantitatively evaluated via chromatography was detected in samples of the ‘Searles’ cranberry cultivar. In the studied cranberry samples, the predominant phenolic compounds were hyperoside, quercetin, and procyanidin A2, while the amounts of other compounds were significantly lower. HCA and PCA revealed that ‘Woolman’, ‘Holliston’, ‘Pilgrim, and ‘Searles’ fruit samples had different quantitative content of phenolic compounds from other cranberry cultivars. Meanwhile, fruit of ‘Baiwfay’, ‘Drever’, ‘Bain’, and ‘Bergman’ were similar in their phytochemical profile.

Highlights

Over the last three decades, the use of botanical pharmaceutical preparations and dietary supplements has increased significantly and over 80% of the population worldwide relies on the effectiveness of botanical pharmaceuticals in primary healthcare [1]
The results of this study will provide new knowledge about the composition and content of phenolic compounds in fruit of American cranberries cultivated in Lithuanian climatic conditions and the antioxidant activity of their extracts in vitro, which will give a wide range of possibilities to employ these plants as a source of phenolic compounds
During pilot spectrophotometric evaluations, the highest total amount of phenolic compounds was found in American cranberry samples of the ‘Bain’ clone (18.06 ± 0.15 mg gallic acid equivalent (GAE)/g dry weight (DW), p < 0.05), and the highest total amount of flavonoids was detected in the ‘Drever’ and ‘Baiwfay’ cultivars

Summary

Introduction

Over the last three decades, the use of botanical pharmaceutical preparations and dietary supplements has increased significantly and over 80% of the population worldwide relies on the effectiveness of botanical pharmaceuticals in primary healthcare [1]. Organization, traditional medicine is an important and frequently underestimated part of healthcare. A document passed in 2013 regulates the strategy of the use of traditional medicine for the period of. The aim of this strategy is to help the member states use the potential positive input of traditional medicine in disease prevention and treatment and to stimulate safe and effective use of traditional medicine [2]. Plants that accumulate higher amounts of biologically active compounds have gained popularity. American cranberry (Vaccinium macrocarpon Aiton) is one such highly promising plant

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