Variabilities on DNA Level of Two Selected Potato Genotypes under Seawater Salinity

Abstract

Random Amplified Polymorphic DNA (RAPD) banding patterns of selected potato (Cara) and (Lady Rosetta), under seawater salinity stress, on agarose gel showed (9 and 10) distinct RAPD profiles generated by five of different 10-mers oligonucleotides primers. The recorded RAPD data of both potato genotypes showed highly polymorphism and low levels of similarity between and inside the treated samples and the untreated control sample. The RAPD analysis of the treated plants of Lady Rosetta showed 363 amplified bands of the treated and control plants whereas the treated plants distributed as ~50.41% unique polymorphic bands, ~29.75% of repeated polymorphic bands and ~6.89% of monomorphic bands as compared with the control plants which showed ~8% unique polymorphic bands and ~4.95% monomorphic bands. However, with Cara, the RAPD analysis showed 227 amplified bands of the treated and control plants whereas the treated plants distributed as ~48.02% of unique polymorphic bands and ~31.28% of repeated polymorphic bands and ~4.4% of monomorphic bands as compared with the control plants which showed ~13.22% unique polymorphic bands and ~3.08% monomorphic bands as compared with the treated plants. There is no doubt that, the gain or the loss of parts of the DNA may consider as speculation the reason for salinity tolerance in the presented work. On the other hand, the exposure to the salinity stress leads to induce changes on the DNA level.