Abstract
Aim: Green, accurate and rapid methods, namely LC-MS/MSand thin layer chromatography-densitometric methods, were developed for determination of amlodipine besylate and celecoxibin presence of its process-related impurities, 4-methylacetophenonein pure and formulated tablets. Results: LC-MS/MS was achieved on ZORBAX Eclipse Plus C18 column using methanol:aqueous solution of 5mM formic acid (95:5v/v). High sensitivity with low limit of detectionvalues 0.00028, 0.00027 and 0.0003 for amlodipine, celecoxib and 4-methylacetophenone, respectively were obtained. While, thin layer chromatography-densitometric was established using methanol:water:ammonia (70:25:1.5, by volume). Good linearity was obtained in the range of 0.1-10μg/band, 1-150μg/band and 0.01-2μg/band for amlodipine, celecoxib and 4-methylacetophenone, respectively. Conclusion: The proposed method validation was achieved according to ICH guidelines. Those methods possess advantages of being ecofriendly methods which permit their application in quality control laboratories.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call